Life Sciences Commons^™

Science Fair: Is It Worth The Work? A Qualitative Study On Deaf Students' Perceptions And Experiences Regarding Science Fair In Primary And Secondary School, Vivian Lee Smith

Dissertations

Science fairs have a long history in American education. They play an important role for establishing inquiry-based experiences in a science classroom. Students may be more motivated to learn science content when they are allowed to choose their own science fair topics. The purpose of this study was to examine Deaf college students’ perceptions and experiences regarding science fair participation during primary and/or secondary school and determine the influence of science fair involvement on the development of language skills, writing skills, and higher order thinking skills as well as its impact on choice of a STEM major.

This study examined …

Characterizing The Persistence Of Sewage Enterococci In Mississippi Coastal Waters, Kimberly Anne-Marie Lewis

Master's Theses

Enterococci are microbiological indicators of marine recreation water quality.
Their reliability as fecal indicators is questioned as they are shown to persist in the environment. Multiple laboratory studies on their persistence have been done but few under natural environmental conditions. The purpose of this study was to investigate how long sewage enterococci and enterococcal DNA persist in beach water and to determine whether there is a difference in the genetic diversity and hardiness of sewage vs. environmental isolates. To study persistence, sewage was diluted with beach water, placed in microcosms, and deployed at a beach site in Longbeach and Pass …

Comparative Analysis Of Antiviral Responses In Mouse Embryonic Stem Cells And In Mesenchymal Stem Cells, Jundi Wang

Master's Theses

Embryonic stem cells (ESCs) are cells that have unlimited capacity for selfrenewal and differentiation. These properties make ESCs a great cell source for application in regenerative medicine. When used for cell therapy, ESC-derived cells could be placed in a wounded area that is likely to be exposed to various pathogens. However, it is not well-understood whether ESCs and ESC-derived cells have active antiviral responses against infectious agents from the environment. To answer this important question, I comparatively analyzed the antiviral responses of ESCs and mesenchymal stem cells (MSCs, C3H10Tl/2 cell line) to infectious agents. Using the expression of type I …

Role Of Msa In Immune Evasion, Persistence, And Protease Regulation In The Human Pathogenic Strains Of Staphylococcus Aureus, Maria Deepa Basco

Dissertations

Opportunistic pathogens like Staphylococcus aureus on entering the host can stay colonized at the foci of infection or evade the immune system to disseminate to other sites. In this study we investigated the regulatory influence of the modulator of sarA (msa) on immune evasion and host persistence, employing the hospital-acquired strain S. aureus UAMS-1 and community-acquired strain S. aureus USA300 LAC. In the murine sepsis model, mutation of the msa gene in LAC showed no change in dissemination of infection; however, in UAMS-1 a decrease in microbial load was observed in the lungs. Differential regulation by the msa gene was …

An Insight Into The Microbial Diversity And Expression Of Cysteine Protease Inhibitors (Cystatin) In Rickettsia Parkeri Infected Amblyomma Maculatum, Khemraj Budachetri

Master's Theses

Amblyomma maculatum (Gulf Coast tick) is an emerging tick species of public health significance in United States. It is a competent vector of Rickettsia parkeri, an etiological agent of a human rickettsiosis. In this study, we investigated the spotted fever group of rickettsial diversity in A. maculatum based on rickettsial ompA gene PCR. Our results showed A. maculatum harbors R. parkeri, R. amblyommii, and R. endosymbiont of A. maculatum. While only R. parkeri was detected in female salivary glands which suggest its ability to traffic from midgut to salivary glands via hemocoel. The presence of R. parkeri was further …

Molecular Detection Of Tick-Borne Pathogens Associated With Ixodid Tick Species Infesting Migratory Songbirds And Ruminants, Nabanita Mukherjee

Master's Theses

Ticks are hematophagous arthropods and capable of inoculating various infectious agents to their vertebrate hosts. Ticks attached to birds and ruminants are capable of carrying tick-transmitted microorganisms. In our study, the prevalence of tick-borne pathogens was examined in ticks infesting migratory songbirds and Pakistani ruminants. During spring migration seasons, ticks were removed from northward-migrating songbirds in Louisiana and identified as Amblyomma Jongirostre, Amblyomma nodosum, Amb/yomma ca/caratum, Amblyomma maculatum, and Haemaphysalis species, all are considered as Neotropical ticks. Ticks removed from ruminants were identified as Hya/omma anatolicum, Hyalomma dromedarii, Rhipicephalus microplus, and Rhipicephalus sanguineus. A total of 112 and 72 bacterial …

Development Of A Genetic Monitoring Program For Stock Enhancement Of Mississippi Spotted Seatrout (Cynoscion Nebulosus), Carly Renee Somerset

Master's Theses

The spotted seatrout is a coastal sciaenid fish that is intensively exploited in the northcentral Gulf of Mexico. A stock enhancement research program was initiated in 2004 to develop methods to culture juveniles and release them in natural habitats to increase recruitment in the fishery. The goal of this study was to initiate a genetic monitoring program for stock enhancement activities. Assays for 15 microsatellite loci were optimized. Simulations indicated that this panel of molecular markers was sufficient to identify recaptured hatchery fish with high confidence assuming genetic data on broodfish spawned to produce released fish were available. Stock structure …

Rna Interference-Mediated Depletion Of N-Ethylmaleimide Sensitive Fusion Protein And Synaptosomal Associated Protein Of 25 Kda Results In The Inhibition Of Blood Feeding Of The Gulf Coast Tick, Amblyomma Maculatum, Rebecca Browning, Shahid Karim

Faculty Publications

The signalling pathways in tick salivary glands that control ‘sialo‐secretome’ secretion at the tick−host interface remain elusive; however, this complex process is essential for successful feeding and manipulation of the host haemostatic response. Exocytosis of the sialo‐secretome in the salivary glands requires a core of soluble N‐ethylmaleimide‐sensitive fusion (NSF) attachment proteins (SNAPs) and receptor proteins (SNAREs). SNAREs have been identified as the key components in regulating the sialo‐secretome in the salivary gland cells. In this study, we utilized RNA interference to investigate the functional role of two Amblyomma maculatum SNARE complex proteins, AmNSF and AmSNAP‐25, in the tick salivary glands …

Interventions For Improving Nutrition And Physical Activity Behaviors In Adult African American Populations: A Systematic Review, January 2000 Through December 2011, Jennifer Lemacks, Brittny A. Wells, Jasminka Z. Ilich, Penny A. Ralston

Faculty Publications

Introduction The incidence of preventable chronic diseases is disproportionally high among African Americans and could be reduced through diet and physical activity interventions. Our objective was to systematically review the literature on clinical outcomes of diet and physical activity interventions conducted among adult African American populations in the United States. Methods We used the Preferred Reporting Items for Systematic Review and Meta Analysis construct in our review. We searched Medline (PubMed and Ovid), Cochrane, and DARE databases and restricted our search to articles published in English from January 2000 through December 2011. We included studies of educational interventions with clinically …

Mouse Embryonic Stem Cells Are Deficient In Type I Interferon Expression In Response To Viral Infections And Double-Stranded Rna, Ruoxing Wang, Jundi Wang, Amber M. Paul, Dhiraj Acharya, Fengwei Bai, Faqing Huang, Yan-Lin Guo

Faculty Publications

Embryonic stem cells (ESCs) are considered to be a promising cell source for regenerative medicine because of their unlimited capacity for self-renewal and differentiation. However, little is known about the innate immunity in ESCs and ESC-derived cells. We investigated the responses of mESCs to three types of live viruses; La Crosse virus (LACV), West Nile virus (WNV), and Sendai virus (SeV). Our results demonstrated mESCs were susceptible to viral infection, but they were unable to express type I interferons (IFNα and IFNβ,IFNα/β which differ from fibroblasts (10T1/2 cells) that robustly express IFNα/β upon viral infections. The failure of mESCs to …

Habitat Characterization, Habitat Use And Associated Growth Of Juvenile Spotted Seatrout (Cynoscion Nebulosus) In A Mississippi Bay System: Implications For Stock Enhancement Practices, Joseph Read Hendon

Dissertations

The spotted seatrout (Cynoscion nebulosus) is the most targeted recreational fishery species in Mississippi coastal waters. Consequently, the Seatrout Population Enhancement Cooperative stock enhancement program was initiated to investigate methods for supplementing this heavily exploited population. Given ecological data needs for both wild and hatchery-reared (HR) juveniles of the species, habitat mapping, directed sampling and a caging study were conducted. Submerged aquatic vegetation (SAV), oyster shell and non-vegetated substrates were mapped at water depths < 2 m in Point aux Chenes Bay, Mississippi, using a sidescan sonar, which provided more accurate estimations of habitat coverage, particularly for SAV, compared to point-based sampling. Applying habitat strata delineated from sidescan mapping, late-juvenile spotted seatrout [125 - 275 mm total length (TL)] were sampled in SAV, marsh-edge and non-vegetated habitats as part of a seasonal gill net survey conducted in 2011 and 2012. Spotted seatrout ranging from 138 to 485 mm TL were collected using a smallmesh gill net, and 65% of collected individuals fell within the targeted late-juvenile size range. Overall mean catch-per-unit-effort of C. nebulosus did not differ for abundance or biomass between years, but both metrics were significantly higher in SAV habitats. Employing cage enclosures that …

Community Metrics And Trophic Dynamics In Tidal Creeks In An Anthropogenically Fragmented, Coastal Landscape, Michael Robert Lowe

Dissertations

Salt marsh landscapes are among the most anthropogenically altered ecosystems in the world. Urbanization (i.e., accumulation of impervious cover and man made structrues) of the coastal landscape can disrupt the delivery of numerous ecosystem services. Among the many services provided by salt marsh habitats, they serves as the primary habitats for distinct macroinfauna (i.e., benthic and epibenthic macrofauna) and nekton (i.e., fish and decapod crustaceans) assemblages. In this dissertation, I used a number of metrics to test the overarching hypothesis that coastal urbanization has negative consequences for salt marsh faunal assemblages. Chapter I uses a landscape ecology approach to show …

Evaluation Of Human Fecal Pollution In Mississippi Coastal And Creek Waters Using Library Independent Markers, Christopher John Flood

Dissertations

The objective of this study was to determine whether statistically valid correlations could be elucidated between standard indicator bacteria (enterococci and fecal coliforms) from coastal creek and marine samples and the presence of four library independent molecular markers that are human or sewage specific. Eight hundred and nineteen samples were collected between August 2007 and July 2010 to determine enterococcal and fecal coliform counts and the presence of genetic markers for sewage indicator organisms Methanobrevibacter smithii, human specific Bacteroides sp., Bacteroides thetaiotaomicron, and Fecalibacterium sp. During the course of this study environmental parameters were measured and statistically analyzed to determine …

Molecular Characterization And Functional Significance Of The Vti Family Of Snare Proteins In Tick Salivary Glands, Ashley M. Villarreal, Steven W. Adamson, Rebecca E. Browning, Khemraj Budachetri, Muhammad Sohail Sajid, Shahid Karim

Faculty Publications

Exocytosis involves membrane fusion between secretory vesicles and the plasma membrane. The Soluble N-ethylmaleimide-sensitive factor attachment proteins (SNAPs) and their receptor proteins (SNAREs) interact to fuse vesicles with the membrane and trigger the release of their sialosecretome out of the tick salivary gland cells. In this study, we examined the functional significance of the Vti family of SNARE proteins of blood-feeding Amblyomma maculatum and Amblyomma americanum. Vti1A and Vti1B have been implicated in multiple functional roles in vesicle transport. QRT-PCR studies demonstrated that the highest transcriptional expression of vti1a and vti1b genes occurs in unfed salivary glands, suggesting that …

Socio-Ecology Of The Marsh Rice Rat (Oryzomys Palustris) And The Spatio-Temporal Distribution Of Bayou Virus In Coastal Texas, Tyla S. Holsomback, Christopher J. Van Nice, Rachel N. Clark, Alisa A. Abuzeineh, Jorge Salazar-Bravo

Faculty Publications

Along the southeastern coast of the United States of America (USA), the marsh rice rat (Oryzomys palustris) is the primary host for the hantavirus genotype Bayou. According to the socio-ecological model for a territorial, polygamous species, females should be distributed across space and time by habitat resources and predation risks, whereas males should space themselves according to the degree of female aggregation and reproductive synchrony. To investigate how females affect the male-male transmission paradigm of Bayou virus, rodents were captured, marked, released, and re-captured in two macrohabitat types across a 30-month period. Microhabitat cover variables were quantified around …

The Trap-Neuter-Return Program As A Means For Collecting, Analyzing, And Comparing Data In Animal Care Facilities In Florida, Louisiana, And Mississippi, Stephanie T. Doty

Honors Theses

The TNR program has been met with both scrutiny and approval as a result of conflicting data from studies that examine its usefulness as a means of controlling the free-roaming cat population explosion (Levy & Crawford, 2004). Though the differing opinions will be discussed, it is not the intention of this paper to provide evidence in support of one or the other; instead, the purpose is to examine the various clinics’ implementation of the TNR program and its usefulness in providing data. In addition, an analysis of the data was done to determine what they may suggest about the free-roaming …

Attempt To Synthesize The (S) Enantiomer Of A Glutathione Analog To Be An Inhibitor Of Glutathione Reductase, Sidney L. Mcclendon

Honors Theses

Malaria is a disease of the blood that is caused by a parasite and affects over forty percent of the world population today. This small parasite can be treated in a very short period of time; however, there are many countries that do not have access to advanced medicine. This means that the people infected with this disease will usually die from complications arising from the illness. Malaria is prevalent in tropical and subtropical countries close to the equator where more than half of the world’s population resides. Dominance of malaria in these areas is because of the low economic …

An Examination Of The Social Functions Of Mouth Behaviors In Bottlenose Dolphins (Tursiops Truncatus), Kirsten E. Lapuyade

Honors Theses

This project examines the social functions of mouthing behaviors in bottlenose dolphins (Tursiops truncatus). The Roatan Institute for Marine Sciences (RIMS) houses dolphins which are captive but in a natural habitat. The twenty-six dolphins are used in human interactions, but for the rest of their time they are allowed to roam naturally. These dolphins make it possible to examine natural social behavior in a captive setting. The following study examines video data of these dolphins obtained by Dr. Stan Kuczaj in 2009. Behavior of the dolphins in these videos was recorded, the primary focus being mouthing behaviors.

There …

Determination Of 14c-Acetate Incorporation To Fungal Mass Conversion Factors Of Selected Saprophytic Fungal Organisms, Jakayla M. Harrell

Honors Theses

A growing body of evidence has emerged over the last several decades on the usefulness of the fungal sterol, ergosterol, in quantifying fungal biomass within decaying plant litter and the technique for measuring instantaneous growth rates of fungi via rates of [¹⁴C]-acetate incorporation into ergosterol. Although popular, an underlying problem with these methods is the paucity of studies that have determined conversion factors that relate the precursor molecules to meaningful measurements of fungal biomass and growth rates, respectively. This research was conducted to establish conversion factors relating rates of ¹⁴C-acetate incorporation to rates of fungal mass synthesis …

Characterization Of The Function Of A Mold Specific Gene, Ms95, In The Pathogenic Dimorphic Fungus, Histoplasma Capsulatum, Danielle J. Williamson

Honors Theses

Histoplasma capsulatum is a dimorphic fungus that causes the respiratory disease histoplasmosis. At 25°C, the fungus grows as a multicellular mold in soils contaminated by bird and bat excreta. Once the soil is disturbed, spores are released and inhaled into the lungs. The fungus shifts to a unicellular, pathogenic yeast within the lungs at 37°C. Our laboratory’s main objective is to characterize the genes that are involved in the mold-to-yeast dimorphism. This study focuses on the mold-specific gene, MS95. According to The Broad Institute Histoplasma capsulatum GenBank Blastx search, MS95 has several homologs, including the well-studied Saccharomyces cerevisiae. …

Intergenic Regions And Repeating Gene Sequences In The Dinoflagellate Karenia Brevis, Colleen A. Gregg

Honors Theses

Karenia brevis is a species of dinoflagellates that is responsible for “red tides” (more formally known as harmful algal blooms) found in the Gulf of Mexico. The blooms have adverse effects on humans and marine animals. Karenia brevis produces toxins that, in humans, can cause upper and lower respiratory problems as well as nausea and vomiting. For marine mammals, birds, fish, and other marine organisms, exposure to brevetoxins can result in morbidity and even death.

Even with the technology and resources we have today, scientist do not fully understand the mechanisms behind toxin production and bloom initiation. Because cellular processes …

Comparative Effects Of Upper Or Lower Body Ergometry To Facilitate Recovery From High-Intensity Combined Arm And Leg Exercise, Benjamin Chad Lyman

Honors Theses

The purpose of this study was to compare the effects of upper body versus lower body ergometry on blood lactate concentration ([La]) disappearance. Ten individuals (age: 20.6 ± 1.3 yrs, height: 1.72 ± 0.08 m, weight: 66.77 ± 10.42 kg) completed preliminary testing sessions, 3-5 days apart, to determine the power output corresponding with the subject’s onset of blood lactate (OBLA), which for the purposes of this study is considered the subject’s lactate threshold (LT), for leg ergometry (LT_L) and arm ergometry (LT_A). Participants then returned to the laboratory on three separate occasions to complete the …

Exploring The Interactions Between Interleukin-8 (Il8) And Amyloid-Β (Aβ) Peptide In Traumatic Brain Injury, Olivia G. Gibson

Honors Theses

Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by cognitive dysfunction and memory loss, and pathologically characterized by amyloid-beta (Aβ) plaques and inflammation. Recent research has shown that these Aβ plaques are also found in traumatic

brain injury (TBI) patients. This discovery has led to a proposed pathway leading from traumatic brain injuries to dementia, more specifically AD. After a TBI, human granulin-A (GRN) and interleukin-8 (IL8) are released and the number of elastases in the brain increases in response to the inflammation response. IL8 is a cytokine that is released in acute inflammation responses, and the levels of IL8 …

Plasmodium Prevalence In Northern Cardinals Over An Eight Month Period, Kaylee M. Gentry

Honors Theses

This study implemented microscopic assays on field collected data to assess the prevalence of avian malaria in a non-migratory species, Cardinalis cardinalis. Following capture, blood smears were collected from birds at the University of Southern Mississippi’s Lake Thoreau Environmental Education and Research Center over an eight month period from September 2012 to March 2013. These smears were then stained using a Hema 3 stain set and microscopically assessed for the presence of Plasmodium relictum (avian malaria). Stained blood smear samples from a migrant species, Junco hyemalis, were used for comparisons of malarial infection. Additionally, corticosterone (CORT) assays were …

Targeted Delivery Of Ribonucleotide Reductase Sirna In Cancer Cells Via Gold Nanoplexes, John Caleb Grenn

Honors Theses

Cancer treatment has much room for improvement, as therapies today cannot differentiate well between what cells are part of a tumor and which are healthy. Our research involves targeting cancer cells by their overexpressed folic acid receptors, and delivering small interfering RNAs (siRNAs) to silence genes crucial to cell survival by the RNA interference gene knockdown pathway. By using Gaussia luciferase siRNA (siGLuc) as a model for cell delivery, we have been able to test our ability to deliver siRNA via a gold nanoparticle delivery system, also developed in our lab. We also synthesized ribonucleotide reductase siRNA (siRRM2), which will …

Cloning And Expression Of The Cbbo (0910) Gene From Halothiobacillus Neapolitanus And Its Potential To Code For Rubisco Activase, Emily F.E. Bustin

Honors Theses

Ribulose-1,5-bisphosphate carboxylase/oxygenase or RubisCO is an enzyme found in autotrophic organisms that functions to fix CO₂ and therefore plays an important role in the global carbon cycle. In order for RubisCO to increase its slow enzymatic rate, it must be exposed to high amounts of its substrate, CO₂. Carboxysomes, which sequester the RubisCO and its substrate, provide this function. It was previously believed that all the components necessary for carboxysome function were encoded by genes within the traditional cso operon. Recently, however, a gene in an operon located downstream of the cso operon was found to encode …

Arthropod Density In A Fragmented Urban Landscape Along The Northern Coast Of The Gulf Of Mexico, Anna E. Williams

Honors Theses

Ecologists once focused their research on “pristine” habitats that were considered untouched by human activity. As urbanization rapidly increases, the concept of pristine habitats becomes obsolete. Urban habitats must be studied in order to understand the ecology of our increasingly developed society. Rapid urbanization greatly affects coastal habitats. Popular real estate, strip malls, casinos, and resorts all fragment urban landscapes. Much of the northern coast of the Gulf of Mexico is a fragmented urban landscape caused by rapid development. That same coastal landscape is ecologically important and includes habitats important to many different organisms, among them intercontinental migratory songbirds that …

Characterization Of Whole Pine Tree Substrates For Adventitious Rooting Of Cuttings And Initial Growth Of Seedlings, Anthony Lynn Witcher

Dissertations

Processed whole pine (Pinus taeda L.) trees have been extensively evaluated to supplement peatmoss and pine bark usage in container substrates for greenhouse and nursery crop production. The suitability of whole pine tree (WPT) substrates for crop propagation has not been investigated. Demonstrating the versatility of WPT substrates is essential to expanding their commercial availability and use.

The objective of this work was to evaluate WPT substrates for stem cutting and seed propagation of ornamental crops, and to identify factors affecting root development. Stem cutting and seedling root development was evaluated in WPT and traditional (peatmoss and pine bark) …

Mississippi's First Annual Farm To School Week: Evaluation Of School Participation, Kelsey Jean Lingsch

Master's Theses

Mississippi has recently established the first week of October as statewide Farm to School (F2S) Week, providing Mississippi the opportunity to improve the health status of children while improving the local economy. The study aimed to measure Mississippi school districts' participation during F2S Week 2012 and identify perceived barriers and future interest of participation among child nutrition directors (CND). An electronic survey was sent to the 156 CNDs in the state of Mississippi participating in the National School Lunch Program. Data were analyzed in SPSS v.20 using descriptive and correlational statistics. Among the 75 CNDs who responded to the survey, …

A Bird In The Binoculars: Understanding Birdwatchers' Potential To Contribute To Sustainable Development And Biological Conservation, Sheri Lynn Glowinski

Dissertations

The three studies comprising this research drew from local economic development and environmental sociology to understand aspects of human dynamics that influence the conservation of birds. Using survey data collected from birdwatchers visiting coastal Alabama, the first study examined the local economic impact of birdwatching tourism and the factors that participants deemed important with respect to their recreation. Birdwatchers spent $103,305 during the four month study period. The total annual economic impact of this recreation on the area was estimated at $937,470, producing a multiplier of 1.48 and the equivalent of 20.3 full time jobs. Infrastructural variables (parking, bathrooms, site …