Life Sciences Commons^™

Oyster Drill (Stramonita Haemastoma Complex) Habitat Preferences In A Shallow Coastal Estuary, Coen Mcgarrah, Cynthia Lupton, Eric Sparks, Jennifer L. Debose

Gulf and Caribbean Research

Southern oyster drill snails (Stramonita haemastoma complex) are nuisance organisms within the Gulf of Mexico. This is due to their damaging impact on recovering and farmed oyster (Crassostrea virginica) populations, which are one of the drills’ main food sources. Oyster reef recovery and increased aquaculture production are priorities for Mississippi and the wider northern Gulf coast. The Grand Bay National Estuarine Research Reserve (NERR) hosts multiple oyster reef restoration sites within its boundaries, yet no comprehensive survey of oyster drill abundance has been conducted in this area to date. Additionally, knowledge of oyster drill preferences for sediment …

First Photo-Identification Resighting Of A Male Sperm Whale (Physeter Macrocephalus) Between Guadeloupe, Lesser Antilles And Andøya, Northern Norway, Rocío Prieto González, Lisa Steiner, Zoë Morange, Tiu Similä, Marten Brill, Cédric Millon, Laurent Bouveret

Gulf and Caribbean Research

No abstract provided.

Pollutant Load Reduction In Sarasota Bay, Florida (Usa): Target Development, Implementation, And Outcomes, David Tomasko, Mike Wessel, Jon Perry, Chris Anastasiou, Ryan Gandy

Gulf and Caribbean Research

Between 2006 and 2012, seagrass coverage across Sarasota Bay expanded by 1,108 ha, a 28% increase. During the subsequent years of 2012−2020, 830 ha of seagrass were lost, a 16% decline. In response to these findings, resource management efforts focused on determining the likely reason(s) why those 2 periods had such contrasting trends, to come up with strategies to reverse these losses. Based on results from the seagrass data, the 7—y period of 2006−2012 was designated a “reference period” and water quality data were compared against the subsequent 7—y designated “degraded period” of 2013−2019. Annual average chlorophyll-a (chl-a …

Non-Native Seagrass, Halophila Stipulacea, Reaches Northern Caribbean, Elizabeth R. Whitman, Elizabeth W. Stoner, Stephanie K. Archer

Gulf and Caribbean Research

Halophila stipulacea, a seagrass native to the western Indian Ocean, has become an established invader throughout the western Atlantic, where it often outcompetes native seagrass species across diverse environmental conditions. Here, we report its presence in the northern Bahamas, ca. 2300 km northwest of the first Caribbean record in Grenada and 304 km northeast of the recent observation in Florida, USA. This range expansion represents the northernmost Caribbean record to date and highlights the species’ capacity for long-distance dispersal and continued spread within the region. Our observation underscores the need for coordinated monitoring efforts to track the trajectory of …

Notes On The Ichthyofauna Associated With Artisanal Fishing In A Severely Impacted Estuary In The Southwestern Gulf Of Mexico During The Rainy Season, Rodrigo Vivanco-Cadena, César Meiners-Mandujano, Roberto Martín Cruz-Castán

Gulf and Caribbean Research

No abstract provided.

Manatee Dock Sign Distribution And Its Influence On Publicly-Sourced Sighting Reports In Alabama, Daniela Payán Arias, Sophia C. Corde, Ruth H. Carmichael

Gulf and Caribbean Research

No abstract provided.

A Marine Science Career In The Eastern Caribbean: Witnessing Four Decades Of Change, Hazel A. Oxenford

Gulf and Caribbean Research

Reflecting back on a long and rewarding career from 1980—2023 at the University of the West Indies, based on the small island of Barbados, this paper tracks the many and often remarkable changes in the marine environment, in attitudes and policies, and available opportunities that have influenced my journey as a marine scientist in an ever—changing but resilient Caribbean. From free—access fisheries to degraded stocks, from coral to algal dominated reefs, from flyingfish to Almaco Jacks, from sandy beaches and clear water to a Sargassum crisis — these are just some of the fundamental changes in the marine environment that …

Fungal Biodiversity, Microbial Communities, And Ecosystem Function In Non-Perennial Streams, Charles T. Bond

Dissertations

A global majority of steams are non-perennial, drying at some point in time and space. Despite the vital roles that fungi and other microbes play in stream ecosystems, most research on stream microbiomes comes from perennial (continuously flowing) streams. The present series of studies addresses this research gap by examining microbial community composition and function in non-perennial streams, with an emphasis on fungi. Chapter I reviews existing scientific literature on the ecological processes that shape microbiome structure and function in non-perennial streams and outlines the varieties of fungi that may live there. Chapter II uses rDNA metabarcoding to survey fungal …

Seagrass Carbon Standing Stock In The Northern Gulf Of Mexico: The Role Of Seagrass Beds In Mississippi As Potential Blue Carbon Sinks, Devan Hildebrand

Master's Theses

Organic carbon stored in marine ecosystems is known as blue carbon. Seagrass beds have the ability to contribute to global blue carbon standing stocks through the uptake and sequestration of carbon from the water column. Through coastal management and conservation best practices, blue carbon habitats have the potential to help mitigate the effects of climate change. Although widely studied, knowledge gaps exist on the sediment carbon standing stock of seagrasses. I aim to better understand how persistent seagrass meadows surrounding barrier islands in the northcentral Gulf of Mexico contribute to long-term blue carbon standing stocks. Subaqueous sediment cores were collected …

Life History And Distribution Of Two Species Of Cownose Ray, Rhinoptera Bonasus And Rhinoptera Brasiliensis, In Mississippi Coastal Waters, Anna Millender

Master's Theses

Historically, the American Cownose Ray (Rhinoptera bonasus) was considered the only rhinopterid residing in the northern Gulf of Mexico (GOM); however, recent studies have confirmed the presence of the Brazilian Cownose Ray (Rhinoptera brasiliensis) throughout this region. The current study investigated the life history and distribution of both species within Mississippi waters, an area that previously represented a data gap for both species. From April 2016 – July 2025, 193 R. bonasus and 94 R. brasiliensis were analyzed to describe the reproductive biology. Reproductive timing, mating, gestation length, and parturition were similar among species. Although more …

Isolation Of The 2019 Bonnet Carré Spillway Effects On The Habitat Suitability Of Brown Shrimp (Farfantepenaeus Aztecus) And Blue Crab (Callinectes Sapidus) Using A Modeling Approach, Mallory Dyson

Master's Theses

In 2019, an unprecedented flood year, the Bonnet Carré Spillway (BCS) was opened on two separate occasions (February 27^th to April 11^th, and May 10^th through July 27^th) diverting a volume 6 times the volume of Lake Pontchartrain into the surrounding coastal waters of Louisiana, Mississippi, and Alabama. The goal of this research was to determine the spatio-temporal influence of these freshwater releases on two economically and ecologically important species (Farfantepenaeus aztecus and Callinectes sapidus) with variable responses to salinity and temperature depending on the age class. The Habitat Suitability Index (HSI) …

Near-Infrared-Spectroscopy-Derived Oxygen Desaturation Rates During Post-Exercise Circulatory Occlusion As Predictors Of Cardiorespiratory Fitness Markers, Carstell Lee

Honors Theses

Cardiorespiratory fitness, measured as maximal oxygen consumption (VO₂max) is a critical predictor of all-cause mortality and overall cardiovascular health. Metabolic carts are the current gold standard for evaluation of VO₂max, but are inaccessible to most facilities and individuals, and are expensive ($20,000+). Near-Infrared spectroscopy (NIRS) has emerged as an accessible and affordable ($5,000-$10,000) alternative for evaluating skeletal muscle oxygenation kinetics and mitochondrial oxidative capacity. However, there is limited research in examining whether NIRS-derived oxygen desaturation rates can be a predictor of cardiorespiratory fitness markers (such as VO₂max and peak power output [PPO]), and whether these measurements show a superior predictive …

Analyzing The Impact Of Diet On Dusky Gopher Frog (Lithobates Sevosus) Development In Head Start Programs, Mckalyn Nelson

Honors Theses

Species are constantly impacted by human activity, directly or indirectly. One critically endangered species under investigation is the dusky gopher frog (Lithobates sevosus), which is an endemic species of frog found in the southeastern United States. Historically found from Louisiana to Alabama, a drought in 2002 narrowed the dusky gopher frog range even further. Head start programs have been shown to be effective in increasing population numbers; however, there is not much published data on the effectiveness of captive rearing diets for these programs. The dusky gopher frog head start program at the University of Southern Mississippi’s Lake …

Expression, Purification And Characterization Of Full Length Tdp-43, Danya Abouzaid

Honors Theses

TAR DNA-binding protein 43 (TDP-43) is a 43 kDa RNA-binding protein linked to several neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), and Alzheimer’s disease. 5-7,28,30,33 Studying full-length TDP-43 has been difficult because of its intrinsic instability and tendency to aggregate.19,25,28,33,36 In this study, we focused on overcoming the difficulties to express full-length TDP-43 in E. coli recombinantly. We focused on expressing full-length TDP-43 as a fusion construct with a maltose-binding protein (MBP) to improve solubility and stability. Our strategy was to purify TDP-43-MBP fusion and make biomolecular condensates with RNA before cleaving the tag with thrombin protease. …

Investigating The Cardiovascular And Autonomic Responses To Energy Drink Consumption: The Role Of Energy And Calorie Content, Sophia Schmidt

Honors Theses

Energy drinks (EDs) are among the most consumed functional beverages, often marketed to enhance focus, alertness, and physical performance. Despite their popularity, the combined cardiovascular and autonomic effects of caffeine and sugar during exercise remain poorly understood. The purpose of this study was to examine the acute cardiovascular, metabolic, and autonomic responses to sugar-sweetened (ED-S), non-nutritive sweetened (ED-NNS), and placebo beverages during submaximal cycling in healthy young males.

Nine male participants (age 22.2 ± 2.4 yrs; BMI 26.2 ± 2.9 kg/m²) completed three experimental visits in a randomized, repeated-measures design. Each visit involved ingestion of one drink (ED-S, ED-NNS, or …

Neural Rrp6 Depletion Drives Circrna Accumulation And Motor Impairment In Drosophila Melanogaster Neurons, Joseph Serio

Honors Theses

Circular RNAs (circRNAs) are a class of ubiquitous eukaryotic RNAs created by back splicing. These noncoding RNAs can modulate gene expression and are implicated in aging and neurodegenerative diseases. To limit the contribution of circRNAs to aging and pathobiology, it is essential to understand how turnover of these molecules is regulated, as it is not clear which enzymes are chiefly responsible for degrading circRNAs in neurons. This study evaluates the roles of ribonucleases potentially involved in neural circRNA turnover using the model fly Drosophila melanogaster. A mifepristone-activated GAL4/UAS GeneSwitch expressed RNAi transgenes and inactivated the ribonucleases. Flies were evaluated on …

Exploring Fisheries Species Distribution Shifts In Response To Freshwater Inflow Through The Bonnet Carré Spillway Using Species Distribution Models, David T. May

Master's Theses

The Bonnet Carre Spillway (BCS) is a flood control structure on the Mississippi River (MR) ~52 km upstream of New Orleans. The BCS protects downstream communities from MR flooding by diverting floodwaters into Lake Pontchartrain when the MR exceeds 5.18m flood stage. Diverted waters flow east into the Mississippi Sound (MSS), introducing turbid, nutrient rich freshwater to the estuary before draining into the Gulf of Mexico. BCS openings have become much more frequent in the last decade, though little work has been done to explore the impact of the BCS on nektonic fisheries species. Here, a series of seasonal Maximum …

Assessment Of A Muscadine Grape (Vitis Rotundifolia) Mapping Population For Tolerance To Pierce’S Disease Pathogen Xylella Fastidiosa And Resistance To Black Rot Disease Pathogen Guignardia Bidwellii, Anushka Tennakoon

Master's Theses

Pierce’s disease (PD) and black rot disease cause severe economic losses for grape production under humid and warm conditions. Identifying resistance genes is crucial for controlling these disease by developing resistant cultivars. Muscadine grape (Vitis rotundifolia) cultivar ‘Pam’ has been identified to possess a high level of resistance to diseases. To elucidate the genetic basis of the resistance to the causal pathogens Xylella fastidiosa and Guignardia bidwellii in this cultivar, parents and 141 F1 individuals developed from open pollinated ‘Pam’ were evaluated for two seasons under natural disease pressure. The mapping population was genotyped using genotyping-by-sequencing (GBS) and …

Applying Transcriptomics For Technology Development And Conservation In North American Marine Organisms, Cory Von Eiff

Dissertations

RNA biology is a rapidly expanding field, with many avenues of discovery among marine organisms. In this study, we analyze both mollusks and sawfish. First, we perform an evaluation of sRNA biogenesis pathways throughout Mollusca, with emphasis on the easter oyster, Crassostrea virginica. Understanding these molecules prescribes RNAi-based gene silencing approaches, benefiting genetic investigation and biotechnology. Similar to other animal groups, mollusks have conserved microRNAs (miRNAs), with some shared with ecdysozoans and deuterostomes; however, there is no evidence of an endogenous small interfering RNA (siRNA) pathway. These results suggest that long double-stranded RNA (dsRNA)-based RNAi is not appropriate for gene …

Cationic Conjugated Polymers With Enhanced Doped-State Planarity For N-Type Organic Thermoelectrics, Jiu-Long Li, Xin-Yu Deng, Jupeng Chen, Peng-Xiang Fu, Shuang-Yan Tian, Yunfei Wang, Xiaodan Gu, Ting Lei

Faculty Publications

To date, extensive efforts have been devoted to designing new conjugated polymers with long alkyl or ethylene glycol sidechains. However, these sidechains are insulators, limiting further performance enhancement in doped conjugated polymers. Moreover, the most widely used chlorinated solvents are toxic, limiting the practical applications of many conjugated polymers. Here, we report a water/alcohol processable n-type conjugated polymer P(Py2FT), featuring side chain-free cationic backbones. P(Py2FT) exhibits a high n-type electrical conductivity of up to 28.1 S cm⁻¹ and a high thermoelectric power factor of up to 28.7 μW m⁻¹ K⁻², comparable to some conventional …

Impact Of Temperature And Microbial Infection On The Honey Bee Hemocytes, Michael E. Oeth

Master's Theses

Introduction

Honey bees (Apis mellifera) rely on hemocytes for immunity, wound healing, and hemolymph homeostasis. While 32°C reflects optimal hive conditions, suboptimal temperatures (22°C) and stressors like pathogens (Varroa destructor, bacteria) or pesticides impair hemocyte function. Using clodronate liposomes (CLD) to deplete hemocytes, we assessed how immune suppression interacts with these stressors.

Methods

Newly emerged (5-day-old) and older (15-day-old) bees were assigned to: CLD, control liposomes (LP), PBS, injection wound, or no treatment. Cohorts were exposed to 32°C (optimal) or 22°C (stressful for non-immunosuppressed bees). Fifteen-day-old bees were challenged with Escherichia coli or Staphylococcus aureus. Survival, hemolymph volume, and hemocyte …

Population Estimates, Demography, And Habitat Assessment Of The Pearl River Map Turtle (Graptemys Pearlensis) In The Pearl River Drainage Of Mississippi, Noah Devros

Master's Theses

The Pearl River map turtle (Graptemys pearlensis) is a highly riverine Emydid endemic to the Pearl River drainage of central and southern Mississippi and southern Louisiana. First described in 2010, G. pearlensis was listed as federally Threatened in 2024. . Due to its relative novelty as a species, no rigorous site-specific population estimates of G. pearlensis in Mississippi have been conducted. The primary goal of this study was to implement a replicable method of assessing the population of G. pearlensis throughout the Pearl River drainage system of Mississippi. A secondary goal was to describe habitat associated with G. …

Movement Ecology And Trophic Dynamics In The Northern Gulf Of Mexico: Implications For Fisheries Management, Calvin Chee

Master's Theses

The northern Gulf of Mexico (nGOM) is a diverse and productive region for living marine resources. The region supports lucrative commercial and recreational fisheries ranging from low-trophic-level species, such as penaeid shrimps and forage fishes, to high-trophic-level predators (e.g. Yellowfin Tuna). This thesis contributes to the region’s ecological knowledge, with the primary aim of enhancing management efforts and providing direct management advice to two important fisheries. In Chapter 1, we develop a traditional per-recruit model for Southern Flounder, as well as a spatiotemporally integrated per-recruit model that incorporates the spatial distribution of fishing effort and the stock’s movement ecology. Fish …

Supplementation Of Magnesium Glycinate: Testing The Acute Effects Of Magnesium Glycinate On The Stress Levels Of College Students, Meagan Bubeck

Honors Theses

he usage of supplements and vitamins to increase health has become a common practice in the United States. However, the true effects are not always well-known and can differ across brands, population, and other factors. In college, many students are functioning in high stress conditions, and some may result to going on anti-anxiety or depression medications. To see if Magnesium supplementation could be a potential first step in improving the stress levels present in the student population, the effects of Mg were studied in a population of 10 participants. Participant data was studied in both an Mg and placebo condition …

Sliding Window Method For Simulating Action Potentials In Axons, Hayden Reed

Honors Theses

ABSTRACT Hodgkin and Huxley’s nonlinear partial differential equations model the excitation and propagation of action potentials in neurons, and there have been numerous attempts at finding the best numerical solution method. This thesis proposes a novel approach to solving these equations: the Sliding Window method, in which a fixed sub-interval is found through capturing the signal’s head and tail. The system is then solved on the sub-interval instead of the entire interval. Using the Sliding Window technique also involves implementing the backward and forward Euler methods and the finite difference method. It will be demonstrated that, in utilizing the Sliding …

Transcriptional Analysis Of Inflammatory Responses Of Mouse Trophoblast Stem Cells To Interferon Gamma, Arnaz Maryam Tariq

Honors Theses

The blastocyst faces significant immunological challenges during implantation, including heightened inflammation, which can contribute to implantation failure. Trophoblast stem cells (TSCs) and their differentiated forms (TSC-TBs), derived from the trophectoderm (TE) of the blastocyst, play a crucial role in immune modulation. Previous studies suggest that interferon-stimulated genes (ISGs) derived from TSC and TSC-TB provide antiviral protection to embryonic stem cells (ESCs). These findings show indication of a potential antiviral role for placental progenitors in shielding the blastocyst. Additionally, TSCs and TSC-TBs exhibit selectively attenuated immune responses to the proinflammatory cytokine interferon-gamma (IFN-γ) compared to mouse embryonic fibroblasts (MEFs). The attenuated …

The Effect Of Implementing A Development Program Using Mental Imagery On The Time To Master Psychomotor Skills, Randal Agee

Dissertations

Psychomotor skills development programs that use mental imagery have been demonstrated to help individuals acquire psychomotor skills (Anu et al., 2020; Seymore-Walsh et al., 2020; Toth et al., 2020). The commercial trucking industry is presently experiencing a global shortage of qualified truck drivers (American Trucking Association, 2019; Staats et al., 2017). One reason individuals are avoiding or leaving careers as commercial truck drivers is the time spent away from home (Staats et al., 2017). A couple logistical strategies have been developed to improve the situation (Mittal et al., 2018; Prime Inc, 2024); however, for the solutions to be sustainable, the …

Unraveling The Antiviral Mechanisms Of Bat Cells: Processing Of Genomic Rnas By Dicer In Bat Cells Limits Sars-Cov-2 Replication, Iyanuoluwani Owolabi

Dissertations

Bats are reservoirs for numerous viruses that cause serious diseases in other animals and humans. Several mechanisms are proposed to contribute to the tolerance of bats to these pathogens. This study investigates the response of bat cells to double-stranded RNA generated by SARS-CoV-2 replication. Here, we found the involvement of Dicer in the processing of viral genomic RNAs during SARS-CoV-2 infection. Examining RNA sequencing of infected cells, small-interfering RNA (siRNA)-like fragments were found derived from viral RNAs. Depletion of Dicer showed a reduction in these RNAs and an increase in viral loads suggesting unlike other mammals, bats may use Dicer …

Determining The Influence Of A Low-Head Dam On Macroinvertebrate Communities In The Bouie River, Natalie Santiago

Honors Theses

The Bouie River is a tributary of the Leaf River and belongs to the Pascagoula River watershed, the latter of which has no flow impediments in the form of sills or dams. The Bouie River has been heavily altered in the lower 10 river kilometers (rkm) from aggregate mining (creating a series of deep gravel pits) and the presence of an earthen and concrete low-head dam (sill) located 6 rkm from the mouth of the river. However, despite these alterations, the immediate stretch of river below the low-head dam is a known spawning habitat for diadromous fish species, including the …

Sibship Analysis Of Fall And Spring Spawn Gulf Sturgeon Eggs In The Choctawhatchee River, Florida, Lyric Mccullouch

Honors Theses

The Gulf sturgeon (Acipenser oxyrinchus desotoi) is an anadromous species that inhabits rivers along the northern Gulf of Mexico. Facing challenges from overfishing and habitat alteration, the Gulf sturgeon is listed as threatened under the Endangered Species Act. This study investigated the genetic distinctiveness of fall and spring spawning groups of Gulf sturgeon in the Choctawhatchee River, a critical habitat for the species. In this study, I used microsatellite loci to analyze Gulf sturgeon eggs previously collected in the fall and spring of 2019 and 2020. The goals of this study were to verify the genetic identity of …