Life Sciences Commons^™

Environmental Perceptions And Objective Walking Trail Audits Inform A Community-Based Participatory Research Walking Intervention, Jamie Zoellner, Jennie L. Hill, Karen Zynda, Alicia D. Sample, M. Kathleen Yadrick

Faculty Publications

Background: Given the documented physical activity disparities that exist among low-income minority communities and the increased focused on socio-ecological approaches to address physical inactivity, efforts aimed at understanding the built environment to support physical activity are needed. This community-based participatory research (CBPR) project investigates walking trails perceptions in a high minority southern community and objectively examines walking trails. The primary aim is to explore if perceived and objective audit variables predict meeting recommendations for walking and physical activity, MET/minutes/week of physical activity, and frequency of trail use. Methods: A proportional sampling plan was used to survey community residents in this …

Determination Of The Spawning Season Of Bigmouth Sleeper In Puerto Rico By Examination Of Gonad Maturation And Reproductive Hormone Cycles, Nathan J. Harris, J. Wesley Neal, Todd D. Sink, Peter W. Perschbacher

Gulf and Caribbean Research

Bigmouth sleepers, Gobiomorus dormitor, are diadromous fish that have potential for hatchery production as both food and sport fish and for conservation purposes. Understanding of bigmouth sleeper maturation and seasonal hormone cycling are necessary in order to realize hatchery production. Therefore, seasonal trends in gonadosomatic index (GSI) and plasma vitellogenin, estradiol, progesterone and total testosterone concentrations were examined in wild and captive populations in Puerto Rico during the presumed spawning season. The spawning season for wild river populations of bigmouth sleepers was protracted over several months, but peaks in male testosterone (6.5 ng/mL) and female vitellogenin (11.3 ng/mL), estradiol …

Effects Of A Severe Cold Event On The Subtropical, Estuarine-Dependent Common Snook, Centropomus Undecimalis, Aaron J. Adams, Jessica E. Hill, Benjamin N. Kurth, Andrew B. Barbour

Gulf and Caribbean Research

The effects of infrequent disturbance events on marine fishes are often difficult to determine, due largely to lack of sufficient pre- and post-disturbance event data. In January 2010, subtropical southwestern Florida (USA) experienced extreme cold for 13 days, which caused extensive mortality of many fish species. The effect of this severe cold event on common snook (Centropomus undecimalis), an economically important gamefish, was assessed using three years (2007-2009) of pre-event and one year (2010) of post-event data from a tag-recapture program conducted over 28 km of Gulf of Mexico barrier islands of Florida. All metrics pointed to a …

Early Growth Of Three Kingfish (Menticirrhus) Species Found In Coastal Waters Of The Northern Gulf Of Mexico, E. John Anderson, Bruce H. Comyns, Harriet Perry, Chet F. Rakocinski

Gulf and Caribbean Research

Southern kingfish (Menticirrhus americanus), gulf kingfish (M. littoralis), and northern kingfish (M. saxatilis) are members of the drum family (Sciaenidae) that are widespread in coastal habitats of the western Atlantic, including in the Gulf of Mexico (GOM). Despite their economic and ecological importance, little is known about growth of young kingfish. Young kingfish were collected from four different Mississippi shoreline habitats in 2005 and 2006; two associated with barrier islands and two along the mainland. Barrier island habitats included surf zones on the south shore and grass beds on the north shore. Mainland habitats …

Flexible Feeding Strategies Of Juvenile Gray Triggerfish (Balistes Capriscus) And Planehead Filefish (Stephanolepis Hispidus) Within Sargassum Habitat, Sarah E. Ballard, Chet F. Rakocinski

Gulf and Caribbean Research

Sargassum-associated juvenile gray triggerfish and planehead filefish exhibited flexible feeding strategies in terms of their use of epifauna or pelagic zooplankton. Four diet samples represented instances of co-occurrence and segregated occurrence. Co-occurring gray triggerfish had the most specialized diets consisting mainly of pelagic copepods and hyperiid amphipods. Conversely, segregated triggerfish as well as both co-occurring and segregated filefish had broader diets mainly consisting of Sargassum epifauna, such as bryozoans, portunid crabs, and hippolytid shrimp. Still, co-occurring planehead filefish also consumed somewhat more zooplankton than segregated planehead filefish. Ontogenetic diet transitions were not as distinct for gray triggerfish as for …

A New Baseline For Diadema Antillarum, Echinometra Viridis, E. Lucunter, And Eucidaris Tribuloides Populations Within The Cayos Cochinos Mpa, Honduras, Paul A.X. Bologna, Lucy Webb-Wilson, Patrick Connelly, James E. Saunders

Gulf and Caribbean Research

We investigated the density of 4 urchin species from 5 shallow reefs in the Cayos Cochinos Marine Protected Area in Honduras. Individual species density varied among reefs with total urchin density ranging from 3.2–7.9 individuals/m². Echinometra viridis (Agassiz, 1863) was the numerically dominant species (2.29/m²) followed by E. lucunter (Linnaeus, 1758) (1.76/m²) with Eucidaris tribuloides (Lamarck 1816) representing the fewest individuals (0.42/m²). Our results indicated that density of Diadema antillarum (Philippi, 1845) differed significantly among reefs (0.4-1.3/m², mean = 0.63/m²), but are three times greater than data collected …

Using The Fishes Of Texas Project Databases And Recent Collections To Detect Range Expansions By Four Fish Species On The Lower Coastal Plain Of Texas, F. Douglas Martin, Adam E. Cohen, Dean A. Hendrickson

Gulf and Caribbean Research

The Fishes of Texas project online database is a large, freely available quality controlled fish occurrence database of museum vouchered specimens. We used data from it, the same project’s separate database of occurrences extracted from published literature and our own recent survey data to examine range stability for four fish species inhabiting the Texas Lower Coastal Plain: Fundulus chrysotus, Fundulus jenkinsi, Heterandria formosa and Poecilia formosa. A weakness of our data is that they consist of presences only and species absences can only rarely be inferred. To help adjust for this we used common widespread species as …

Discrimination Of Juvenile Red Snapper Otolith Chemical Signatures From Gulf Of Mexico Nursery Regions, Michelle Zapp Sluis, Beverly K. Barnett, William F. Patterson Iii, Alan M. Shiller

Faculty Publications

Age-0 red snapper Lutjanus campechanus from the 2005-2007 year-classes were sampled in six regions across the Gulf of Mexico (Gulf) to develop nursery signatures from otolith element : Ca ratios (Ba:Ca, Mg:Ca, Mn:Ca, Sr:Ca, and Li:Ca) and stable isotope delta values (delta C-13 and delta O-18). Element : Ca ratios were analyzed with sector field inductively coupled plasma mass spectrometry on dissolved right sagittae; isotope ratio mass spectrometry was employed to analyze pulverized left otoliths for delta C-13 and delta O-18. Otolith chemical signatures were significantly different among regions in each year. Year-class-specific quadratic discriminant function analysis (QDFA) distinguished nursery …

Mutation At The Human D1s80 Minisatellite Locus, Kuppareddi Balamurugan

Faculty Publications

Little is known about the general biology of minisatellites. The purpose of this study is to examine repeat mutations from the D1S80 minisatellite locus by sequence analysis to elucidate the mutational process at this locus. This is a highly polymorphic minisatellite locus, located in the subtelomeric region of chromosome 1. We have analyzed 90,000 human germline transmission events and found seven (7) mutations at this locus. The D1S80 alleles of the parentage trio, the child, mother, and the alleged father were sequenced and the origin of the mutation was determined. Using American Association of Blood Banks (AABB) guidelines, we found …

A Western Range Extension For Caprella Scaura (Amphipoda: Caprellidae) In The Aransas Bay Ecosystem, Texas, Nicholas L. Ahrens, Faye P. Grubbs

Gulf and Caribbean Research

During March 2009, the skeleton shrimp Caprella scaura and Paracaprella tenuis (Amphipoda: Caprellidae) were collected from several locations throughout the Aransas Bay, Texas ecosystem from Texas Parks and Wildlife fishery—independent trawl and oyster dredge samples. This is a western range expansion for C. scaura; P. tenuis has been reported from this area before. Both species were exclusively associated with a bryozoan, Bugula neritina. Densities of both species ranged between 0.1–3.4 individuals per gram of attached bryozoans. A reproductive population is likely established since several sizes, including adult males and gravid females, were observed. No caprellids were observed after …

Evidence Of Spawning Capable Tarpon (Megalops Atlanticus) Off The Louisiana Coast, William Stein Iii, Nancy J. Brown-Peterson, James S. Franks, Martin T. O'Connell

Gulf and Caribbean Research

Despite the fact that the tarpon (Megalops atlanticus) is a popular sport fish in the Gulf of Mexico (GOM; Ault et al. 2008), little is known of its spawning behavior. Spawning M. atlanticus have never been documented and fertilized eggs have not been observed in situ (Ault et al. 2008). While it has been suggested that adult M. atlanticus move to deep water off the southwest coast of Florida and into the Yucatan Channel to spawn, the actual locations of spawning grounds remain unknown (Smith 1980, Crabtree et al. 1995, Crabtree et al. 1997). Distribution patterns of larvae …

Transcriptomic Profiles Of Peripheral White Blood Cells In Type Ii Diabetes And Racial Differences In Expression Profiles, Jinghe Mao, Junmei Ai, Xinchun Zhou, Ming Shenwu, Manuel Ong Jr., Marketta Blue, Jasmine T. Washington, Xiaonan Wang, Youping Deng

Faculty Publications

Background: Along with obesity, physical inactivity, and family history of metabolic disorders, African American ethnicity is a risk factor for type 2 diabetes (T2D) in the United States. However, little is known about the differences in gene expression and transcriptomic profiles of blood in T2D between African Americans (AA) and Caucasians (CAU), and microarray analysis of peripheral white blood cells (WBCs) from these two ethnic groups will facilitate our understanding of the underlying molecular mechanism in T2D and identify genetic biomarkers responsible for the disparities.

Results: A whole human genome oligomicroarray of peripheral WBCs was performed on 144 …

A Deep Insight Into The Sialotranscriptome Of The Gulf Coast Tick, Amblyomma Maculatum, Shahid Karim, Parul Singh, José M.C. Ribeiro

Faculty Publications

Background

Saliva of blood sucking arthropods contains compounds that antagonize their hosts' hemostasis, which include platelet aggregation, vasoconstriction and blood clotting; saliva of these organisms also has anti-inflammatory and immunomodullatory properties. Perhaps because hosts mount an active immune response against these compounds, the diversity of these compounds is large even among related blood sucking species. Because of these properties, saliva helps blood feeding as well as help the establishment of pathogens that can be transmitted during blood feeding.

Methodology/Principal Findings

We have obtained 1,626,969 reads by pyrosequencing a salivary gland cDNA library from adult females Amblyomma maculatum ticks at different …

State Space Model With Hidden Variables For Reconstruction Of Gene Regulatory Networks, Xi Wu, Peng Li, Nan Wang, Ping Gong, Edward J. Perkins, Youping Deng, Chaoyang Zhang

Faculty Publications

Background: State Space Model (SSM) is a relatively new approach to inferring gene regulatory networks. It requires less computational time than Dynamic Bayesian Networks (DBN). There are two types of variables in the linear SSM, observed variables and hidden variables. SSM uses an iterative method, namely Expectation-Maximization, to infer regulatory relationships from microarray datasets. The hidden variables cannot be directly observed from experiments. How to determine the number of hidden variables has a significant impact on the accuracy of network inference. In this study, we used SSM to infer Gene regulatory networks (GRNs) from synthetic time series datasets, investigated …

Influence Of Detritus Levels And Organic Pollution On Interspecific Resource Competition, Oviposition Behavior, And Larval Survival Of Two Tire-Inhabiting Mosquito Species (Diptera: Culicidae), David Wayne Allgood

Master's Theses

Mosquitoes (Diptera: Culicidae) are vectors of disease in the adult stage, but understanding the factors affecting distributions of the immature stages is important to the understanding and control of adult populations. Discarded automobile tires comprise important larval mosquito habitats. The Asian tiger mosquito (Aedes albopictus) and the southern house mosquito (Culex quinquefasciatus) are two medically important species commonly found in tires, but factors affecting their larval distributions in tires have not been studied, nor have their interspecific interactions. I investigated the effects of chemicals associated with organic pollution on oviposition preferences and larval survival of both …

Tick Infestations And Their Consequences For Migratory Songbirds During Spring Stopover, Johnny Michael Sellers Jr.

Master's Theses

Migratory birds face a number of challenges during their seasonal movement from tropical/sub-tropical Central and South America to more temperate North America. Maintaining health during migration is of particular concern. This study seeks to understand how haematophageous ectoparasites, such as ticks (Ixodida), impact host body condition as they feed on passerines during migration. We hypothesized that foraging location would impact tick acquisition by migrants and that tick burdens during migration would negatively impact body condition. We surveyed 2,064 birds during spring 2009 and 2010 and found that 2.4% of the surveyed birds were infested with one or more …

Thermal Tolerance Of Age-0 Gulf Of Mexico Striped Bass (Morone Saxatilis): Ontogenetic And Genetic Effects, Corey Robert Anderson

Master's Theses

Striped bass, Morone saxatilis, were historically abundant in the Gulf of Mexico region but were largely extirpated from most Gulf rivers by the early 1960s. Since 1967 hatchery stocking has supported populations in Mississippi rivers and to date there is no evidence of natural reproduction. Intolerance of striped bass to high water temperature is a potential factor negatively impacting Gulf striped bass survival and reproductive fitness, and may be a limiting factor to reintroduction of the species in Mississippi. This work contributed to restoration efforts by establishing molecular tools necessary for genetic monitoring of striped bass restoration, and evaluating …

Measuring And Comparing Quantum Yield In Two Species Of Marine Diatoms Subjected To Constant And Fluctuating Light Conditions, Matthew Leon Stone

Master's Theses

A small-scale study was conducted to determine the effects of light fluctuations on the photosynthetic efficiency of marine phytoplankton. Two species, Phaeodactylum tricornutum and Chaetoceros gracile were grown in specialized photobioreactors on a 12-hour:12-hour light:dark cycle. The cultures were diluted 50% daily to attain a specific growth rate of 0.70 d-1. To simulate vertical mixing in high turbidity habitats under various wind conditions, dense cultures were subjected to fluctuating light treatments with frequencies ranging from 0.10 Hz to 2.00 Hz. Parallel experiments subjected the cultures to static light conditions with equal total daily light doses as those of the cultures …

Quantifying Rates Of Autocompaction In The Pearl River Marsh, Louisiana, Jeremiah Nicholas Prouhet

Master's Theses

Annual rates of shallow autocompaction (< 1 m) were calculated in the upper ~one meter of sediment from six cores of a transect in the Pearl River Marsh (PRM), Louisiana, in order to determine the most significant sedimentary property controlling this process. Compaction-free wedge cores were sub-sampled at one centimeter intervals to collect the following data: age control, using 137Cs and 210Pb, and sedimentary variables, specifically, particulate organic carbon, bulk density, median grain size, porosity, and water content. An upper and an underlying sedimentary unit (named the 137Cs and 210Pb units) were defined between radiometric datum levels, and their sedimentary properties were statistically tested for significant differences using the non-parametric Mann-Whitney test. Rates of autocompaction were calculated from the model of Williams (2003), and both rates of autocompaction and sedimentation were tested for significant differences between the upper and lower sedimentary units.

The upper unit in the six cores generally had lower bulk density, higher water content, higher porosity, and higher particulate organic carbon than the underlying unit, but with some interesting exceptions. Additionally, annual autocompaction and sedimentation rates were greatest in the overlying unit at stations PR02 (0.39 ± 0.05 cm/y) and PR05 (0.42 ± 0.03 cm/y) and greatest in the underlying unit at stations PR02 (0.22 ± 0.08 cm/y) and PR03 (0.18 ± 0.06 cm/y). Based on Spearman correlations, rates of autocompaction were most strongly correlated to and influenced by rates of sedimentation rather …

Phytoplankton Abundance And Species Composition In Relation To Environmental Paramenters In Coastal Mississippi Waters, Luz Karime Molina

Master's Theses

Phytoplankton pigments from Coastal Mississippi waters were measured to determine the spatial and temporal distributions and composition of phytoplankton communities. Concentration of phytoplankton pigments were analyzed using High Performance Liquid Chromatography (HPLC) and the compositional changes in phytoplankton communities were determined with CHEMTAX 1.95. Surface water was collected for two years (September 2007-November 2009) at three sampling sites on a monthly basis. The stations were located at the Bay of St. Louis (station 1), the Mississippi Sound (station 4) and the Mississippi Bight (station 8), following a salinity gradient. A time series of the observations documented the variability of different …

The Use Of Computed Tomography To Measure Biogenic Structures In Recently Hypoxic And Normoxic Sediments On The Louisiana Continental Shelf, Valerie Ann Hartman

Master's Theses

Over the past 60 years, seasonal hypoxia in the northern Gulf of Mexico has occurred with increased severity and over a greater area. To determine if biogenic structures in the northern Gulf of Mexico vary in response to hypoxic stress, the seafloor on the continental shelf of Louisiana was analyzed during the spring and fall of 2009 at four provinces of similar sediment type that differ in recent history of bottom water oxygen concentration. Subcores were analyzed by computed tomography (CT) to determine the number, diameter, length, volume, surface area, and depth of biogenic burrow structures in sediments where biogenic …

Movement Ecology Of An Intercontinental Migratory Bird During Spring Stopover, Emily Beth Cohen

Dissertations

Movement ecology is a component of nearly all aspects of animal behavior and an animal’s decision to move is likely influenced by a complex combination of exogenous and endogenous factors. Therefore, an examination of the causes and consequences of organismal movement provides a conceptual framework for understanding complex behavioral strategies. My dissertation research is focused on the movement ecology of an intercontinental migratory songbird during spring migration. I adopted experimental approaches to study the factors influencing how a songbird migrant, red-eyed vireos (Vireo olivaceus), makes decisions in unfamiliar landscapes from the initiation of spring stopover.

I simulated the …

The Effect Of Six Weeks Of Oral Echinacea Supplementation On Erythropoiesis, Nitric Oxide Production, And Exercise Performance, Tyler David Martin

Dissertations

The purpose of the present study was to investigate the effect of six weeks of oral Echinacea supplementation on serum erythropoietin (EPO) and erythropoietic status, plasma nitric oxide [NO], submaximal endurance exercise performance, and maximal oxygen uptake (V˙O_2max). Twenty-four males (mean ± SE): age = 25.2 ± 1.4 yr, height = 178.1 ± 1.4 cm, mass = 78.1 ± 1.6 kg, percent body fat = 12.7 ± 0.9 %, V˙O_max = 52.9 ± 0.9 mL·kg^-1·min^-1 were randomly grouped using a matched-pair, double-blind design and self-administered 8,000 mg·d^-1(5 × 400 mg × 4 …

The Nuclear Pore Complex Mediates Binding Of The Mig1 Repressor To Target Promoters, Nayan J. Sarma, Thomas D. Buford, Terry Haley, Kellie Barbara-Haley, George M. Santangelo, Kristine A. Willis

Faculty Publications

All eukaryotic cells alter their transcriptional program in response to the sugar glucose. In Saccharomyces cerevisiae, the best-studied downstream effector of this response is the glucose-regulated repressor Mig1. We show here that nuclear pore complexes also contribute to glucose-regulated gene expression. NPCs participate in glucose-responsive repression by physically interacting with Mig1 and mediating its function independently of nucleocytoplasmic transport. Surprisingly, despite its abundant presence in the nucleus of glucose-grown nup120Δ or nup133Δ cells, Mig1 has lost its ability to interact with target promoters. The glucose repression defect in the absence of these nuclear pore components therefore appears …

Functional Dissection Of The Glucose Signaling Pathways That Regulate The Yeast Glucose Transporter Gene (Hxt) Repressor Rgt1, David J. Jouandot Ii, Adhiraj Roy, Jeong-Ho Kim

Faculty Publications

The yeast Rgt1 repressor is a bifunctional protein that acts as a transcriptional repressor and activator. Under glucose-limited conditions, Rgt1 induces transcriptional repression by forming a repressive complex with its corepressors Mth1 and Std1. Here, we show that Rgt1 is converted from a transcriptional repressor into an activator under high glucose conditions and this occurs through two independent but consecutive events mediated by two glucose signaling pathways: (1) disruption of the repressive complex by the Rgt2/Snf3 pathway; (2) phosphorylation of Rgt1 by the cAMP-dependent protein kinase (cAMP-PKA) pathway. Rgt1 is phosphorylated by PKA at four serine residues within its amino-terminal …

Refnetbuilder: A Platform For Construction Of Integrated Reference Gene Regulatory Networks From Expressed Sequence Tags, Ying Li, Ping Gong, Edward J. Perkins, Chaoyang Zhang, Nan Wang

Faculty Publications

Background: Gene Regulatory Networks (GRNs) provide integrated views of gene interactions that control biological processes. Many public databases contain biological interactions extracted from experimentally validated literature reports, but most furnish only information for a few genetic model organisms. In order to provide a bioinformatic tool for researchers who work with non-model organisms, we developed RefNetBuilder, a new platform that allows construction of putative reference pathways or GRNs from expressed sequence tags (ESTs).

Results: RefNetBuilder was designed to have the flexibility to extract and archive pathway or GRN information from public databases such as the Kyoto Encyclopedia of Genes …

Bioactive Properties And Clinical Safety Of A Novel Milk Protein Peptide, Richard B. Kreider, Mike Iosia, Matt Cooke, Geoffrey M. Hudson, Chris Rasmussen, Helen Chen, Olof Mollstedt, Men-Hwei Tsai

Faculty Publications

Background: Milk protein fractions and peptides have been shown to have bioactive properties. This preliminary study examined the potential mechanisms of action and clinical safety of novel milk protein peptide ( MP).

Findings: A novel MP mixture inhibits the tyrosine kinase activity of epidermal growth factor receptor ( EGFR), vascular endothelial growth factor receptor 2 (VEGFR2), and insulin receptor (IR) with IC(50) of 9.85 mu M, 7.7 mu M, and 6.18 mu M respectively. In vitro, this multi-kinase inhibitor causes apoptosis in HT-29 colon cancer cells, and in a C. elegans worm study, showed a weak but significant …

Ornamentation, Behavior, And Maternal Effects In The Female Northern Cardinal, Caitlin Winters

Master's Theses

This study seeks to understand the relationship between ornamentation, maternal effects, and behavior in the female Northern Cardinal (Cardinalis cardinalis). Female birds possess ornaments that indicate a number of important known aspects of quality and are usually costly to maintain. However, the extent to which female specific traits, such as maternal effects, are indicated is less clear. It is predicted by the Good Parent Hypothesis that this information should be displayed through intraspecific signal communication. Specifically, androgens and carotenoids are of interest in this study because both are linked to ornamentation, and are also important egg components that …

Feeding Selectivity And Habitat Usage Of Esox Americanus, Scott Richard Clark

Master's Theses

Patterns of piscine prey selection and habitat usage of Esox americanus were assessed through a series of field and experimental studies. Although few detailed studies exist on the foraging behavior and habitat usage of E. americanus, throughout its range it may be an important trophic component in maintaining fish assemblage structure in many lower order streams. Across 17 sampled streams, the presence of E. americanus among reaches within streams was found to be strongly correlated with increasing levels of habitat complexity. Reaches occupied by E. americanus featured increased amounts of in-stream physical structure, increased variability in stream width and …

Mycorrhizal Colonization Of Native Salt Marsh Plants On Mississippi's Gulf Coast And The Effects Of Commercial Mycorrhizal Inoculants On Nursery-Grown Plants, Kathryn Rondot Mcbride

Master's Theses

Salt marshes are important economically and ecologically to the Gulf Coast and other coasts worldwide. Due to human activities, many coastal salt marshes have been degraded or destroyed. Restoration efforts, through the replacement or addition of naturally occurring salt marsh plants, are taking place worldwide. Most restoration plants are raised in nurseries and are not ready for transfer to restoration sites for eight or nine months. Once the plants are at the restoration site many die due to transplant stress. Arbuscular Mycorrhizal Fungi (AMF) may be able to shorten the time the restoration plants need to stay in the nursery …