Life Sciences Commons^™

Out-Of-Pocket Healthcare Expenses Are Associated With Food Insecurity Among South Dakotan Families Who Have Children With Disabilities, Halie E. Kruid

Health and Nutritional Sciences Graduate Students Plan B Capstone Projects

Background
In 2023, 17.9 percent (6.5 million) of households with children experienced household food insecurity, i.e., an inability to acquire enough food to meet household members’ needs. Many socioeconomic factors can influence food security status, such as environmental conditions, social factors, and financial costs. Families with children with disabilities (CWD) may be at higher risk for food insecurity due to additional financial stressors related to these children’s care.
Purpose
The purpose of this study was to determine the prevalence of food insecurity, as well as the medical and financial factors associated with an increased food insecurity prevalence, among South Dakota …

Physiological And Genetic Studies Of Hydrotropism In The Maize Primary Root, Muyu Gu

Electronic Theses and Dissertations

No abstract provided.

Evaluating Dietary Consequences On Body Mass And Thermal Heat Flux Of North American Bison (Bison Bison) Yearling Heifers, Kendall Marie Wald

Electronic Theses and Dissertations

No abstract provided.

Evaluating Effects Of Copper Sources In Nursery Pig Diets And Phytase Inclusion And Diet Interactions On Water Usage In Growing And Finishing Pigs, Daniel Rosales

Electronic Theses and Dissertations

In modern swine production, nutritional strategies that optimize growth performance, resource utilization, and carcass quality are essential. Three independent trials were conducted to evaluate how dietary modifications involving copper (Cu) sources, phytase inclusion, and amino acid reformulation affect nursery and grow-finish pigs. The first trial assessed the effects of three different Cu sources on nursery pig growth performance. A total of 1,145 pigs (5.9 ± 1.3 kg BW) were assigned to one of four dietary treatments: control, tri-basic Cu chloride source #1 (TBCC1), Cu chelate (cCu), or tri-basic Cu chloride source #2 (TBCC2), each providing 150 ppm of Cu. Over …

Deciphering Genetic Contributions To Antibiotic Persistence: Insights From Multi-Gene Knockout Studies, Elise K. Schweer

Electronic Theses and Dissertations

Antibiotic resistance is a critical global health challenge, complicating efforts to treat bacterial infections effectively. Antibiotic persistence plays a significant role in driving resistance. Persisters are a subpopulation of non-dividing bacterial cells that often evade lethal antibiotic treatment. Once antibiotics are removed, these cells "resurrect," resuming growth and may lead to relapsing infections. Notably, the progeny of persister cells exhibit a heightened tendency to acquire mutations or incorporate antibiotic resistance genes, further compounding the challenge of antibiotic resistance. In our recent study, we knocked out seven hypothetical genes and observed a significant reduction in persister levels. Compared to the wild …

Optimizing Heifer Development On Dormant Rangelands By Integrating Precision Livestock Technologies With The Range Supplementation Model And The Evaluation Of Short-Term Rotations Of Precision Technology On Plant Communities, Hadley Ann Dotts

Electronic Theses and Dissertations

Heifer development on dormant rangelands offers a cost-effective alternative to feedlot or drylot systems. However, traditional bunk supplementation methods often result in uneven intake, inefficient feed use, and ecological degradation around static feeding sites. This thesis evaluated the integration of precision livestock technologies and a dynamic Range Supplementation Model to improve individual animal performance, weight uniformity, and rangeland sustainability in extensive heifer development systems in the Northern Great Plains. The first study involved a seven-month trial (November 2023–May 2024) with 50 yearling Angus heifers grazing dormant native rangelands at the SDSU Cottonwood Field Station. Heifers were stratified into two treatments: …

Biotransformation Of Camelina Meal Via Pretreatment And Fermentation For Functional And Nutritional Upgrading, Ram Kumar Bhattarai

Electronic Theses and Dissertations

Camelina sativa, an oilseed crop of the Brassicaceae family, has seen increased cultivation in North America in response to the rising demand for biofuels over the past two decades. With this growth, camelina meal, a byproduct of oil extraction, has emerged as a promising yet underutilized resource, particularly for applications in animal nutrition. This study aimed to evaluate the potential of camelina meal as a substrate for submerged fungal fermentation and to examine how this bioconversion affects its physicochemical and nutritional properties. The influence of mild pretreatments (unwashed, washed only, and washed/dried and ground) on fermentation outcomes was also assessed. …

Target Cell-Associated Cd47 Suppresses Nadph Oxidase Activity In Macrophages During Antibody Dependent Cellular Phagocytosis, Seuly Akter

Electronic Theses and Dissertations

CD47-SIRPα signaling is a well-known immune checkpoint that suppresses macrophage-mediated antibody-dependent cellular phagocytosis (ADCP). In this study, we explored how CD47 influences reactive oxygen species (ROS) production driven by Fcγ receptor (FcγR) signaling. Our results show that macrophages produce significantly more ROS when encountering IgG coated target cells lacking CD47.Polarization with IFNγ further amplified this effect. ThusCD47-SIRPα interactions limit phagocytosis and suppress oxidative ROS production by macrophages. We found that CD47 blockade and IFNγ stimulation enhanced ADCP through distinct mechanisms. CD47 blockade removed the inhibitory "don't eat me" signal supporting ADCP and ADROS. Similarly, IFNγ enhances ADCP by upregulating FcγRI …

Testing The Effect Of Source And Environment On Native Seed Germination To Inform Seed-Based Restoration Of Grasslands, Brandon Clark

Electronic Theses and Dissertations

Grasslands are one of the most endangered ecosystems on the planet, and they provide a variety of important ecosystem services that people rely on. Losses of grasslands can be mitigated through restoration. Seed-based restoration is a regularly used practice due to its practicality and cost-effectiveness. Planting non-local seed that may not be suitable for its destination environment can have consequences in plant establishment and subsequent restoration success. This experiment tested the response (final germination and germination rate) of seed from 11 perennial tallgrass species sourced from 3 different locations to different germination temperature conditions. Seeds from three sources of each …

The Neutral Detergent Fiber And The Acid Detergent Fiber Of The Grass Samples Between Small/Frequent And Large/Infrequent Rainfall Treatments In The C3 And C4 Plant Communities., Zigeng Chen, A. Joshua Leffler

NRM Departmental Data Sets

Data description:
Month: Time of data collection from 2020 to 2022.
Plant community: C3 and C4 plant community.
Treatment: small/frequent (S/F) and large/infrequent (L/I) rainfall treatment.
NDF (g/g DM): The dry matter content of neutral detergent fiber in the grass samples.
ADF(g/g DM): The dry matter content of acid detergent fiber in the grass samples. Cellulose (g/g DM): The dry matter content of cellulose in the grass samples.
Lignin (g/g DM): The dry matter content of lignin in the grass samples.

File type: .csv
File size: 16 KB

Evaluating And Mapping Habitat Suitability And Effects Of Woodland Treatment On Breeding Pinyon Jays In Colorado, Emily Diane Macklin

Electronic Theses and Dissertations

The pinyon jay (Gymnorhinus cyanocephalus) is a highly social, colonially nesting corvid endemic to the interior western United States whose populations have declined by ~72% since 1966, prompting the species’ current review for listing under the Endangered Species Act. Pinyon jays inhabit pinyon pine (Pinus subsect. Cembroides) and juniper (Juniperus spp.) woodlands and other dry conifer woodlands, portions of which are treated through tree thinning and removal for fuels reduction, ecological restoration, and habitat improvement for wildlife. As a result there is pressing need to better understand how woodland treatment and other potential drivers impact pinyon jay habitat suitability and …

Effect Of Vibration Isolation During Transportation On Beef Sirloin Subprimal And Steak Quality, Kylie Nevada Krueger

Electronic Theses and Dissertations

The objective of this study was to determine the influence of vibration during transportation on beef sirloin water holding capacity, color, and tenderness. USDA Choice center-cut sirloin top butts (n = 45 cases) were collected at a commercial processing facility and randomly allocated to one of three treatments 1) regular pallet driven 241 km (Reg241), 2) regular pallet driven 644 km (Reg644), and 3) vibration isolation pallet driven 644 km (Iso644). All subprimals were transported at -2.2 °C (d0) and stored for 10 days to mimic industry practices for product aging. Following storage (d11), one subprimal per case was evaluated …

Improving Reduced Tillage Vegetable Systems For The Northern Great Plains: How Does Early Season Soil Tarping (Solarization And Occultation) Reduce Weeds, Affect Soil Health, And Protect Yield?, Hannah Voye

Electronic Theses and Dissertations

Soil tarping (solarization and occultation) is a no-till weed management practice gaining popularity in the United States for small scale vegetable growers. While many studies have been conducted using variations of this tool in the Northeast United States, minimal research has been done in the windier, sunnier, Midwest climate. Our research explored the effectiveness of early spring solarization and occultation to manage weeds and produce viable onion yield in Eastern South Dakota. Occultation tarps placed in early spring included double sided black and white silage tarps with tarps placed black side up or white side up, each placed 6-, 4-, …

Prospective Use Of Detection Dogs As A Screening Tool For Mycoplasma Ovipneumoniae In Bighorn Sheep (Ovis Canadensis), Michele C. Lovara

Electronic Theses and Dissertations

Mycoplasma ovipneumoniae is a bacterium that can spill over from domestic sheep (Ovis aries) to bighorn sheep (Ovis canadensis), with effects in bighorn sheep ranging from low to no lamb recruitment to whole-herd all-age die-offs from resulting pneumonia. Fast and reliable pathogen or disease detection is essential for managing outbreaks in bighorn sheep and emergences in affected populations. Detection dogs are increasingly being enlisted to close gaps and further complement various disease, pathogen and virus research and response efforts. This thesis explores the use of conservation detection dogs as a potential tool for M. ovipneumoniae surveillance in bighorn sheep through …

Remote Sensing - Based Mapping And Analysis Of Winter Cover Crop Adoption For Sustainable Agriculture, Minnehaha County, Belinda Buechler

Electronic Theses and Dissertations

Winter cover crops planted by farmers such as cereal rye, crimson clover, radishes, hairy vetch, and winter wheat are used to conserve and protect the soil during winter. These crops offer numerous benefits such as improving soil health, reducing erosion, fixing nitrogen, increasing carbon sequestration, and weed suppression. Over time, winter cover cropping has been recognized as a sustainable agricultural practice and has gained attention from Federal and State conservation programs, farmers, and non-governmental organizations. Due to their vital benefits, agencies such as the United States Department of Agriculture (USDA), Natural Resources Conversation Services (NRCS) have partnered with cost-share programs …

Foliar Corn Diseases In South Dakota: Visual Disease Surveys And The Genetic Studies Of Tar Spot And Bacterial Leaf Streak, Ciera Kotaska

Electronic Theses and Dissertations

Corn (Zea mays L.) is one of the most important crops in South Dakota and the United States. Each year, a variety of diseases impact the production of corn in South Dakota, though very little documentation about these diseases exists in the state. This survey documents the foliar diseases of corn and the distribution of each to create a baseline of knowledge. An additional genetic study was conducted to determine the organisms associated with tar spot of corn and bacterial leaf streak (BLS), two emerging diseases in South Dakota. A variety of studies have been conducted on tar spot, caused …

Phytogenic Feed Additives Pre- And Post-Calving And Effects Of Substituting Ddgs With Soybean Meal On Protein Metabolism In Beef Cattle, Grace H. Jardon

Electronic Theses and Dissertations

The objective of these studies was to evaluate nutritional strategies to improve beef cattle productivity through targeted supplementation and protein source manipulation. The first study investigated the effects of a phytogenic supplement (PHYT; a blend of turmeric, capsicum, and black pepper oleoresin) on colostrum yield and composition, calf passive immunity, and cow–calf performance. Twenty-three Angus-based cows were assigned to either a control group or received 250 or 500 mg/head/day of PHYT from 30 days pre-calving to 60 days post-calving. Supplementation with PHYT linearly increased colostrum fat and calf serum IgA concentrations. Milk fat, calf serum IgG, and colostrum IgM tended …

Effect Of Six-Day Germination On Chemical Composition, Functional, And Nutritional Properties Of Pea Varieties, Hojjat Abdollahikhamene

Electronic Theses and Dissertations

Pulses, particularly peas, have long been a crucial source of protein in vegetarian and cost-effective diets worldwide. They have gained popularity in Western diets as more people turn to plant-based proteins as alternatives to animal products. Peas are known for their high protein content; however, their use in food products has been limited due to factors like poor solubility and digestibility. Germination improves the nutritional profile of peas by enhancing protein content and digestibility. The objective of this study was to determine the effects of six days of germination on the nutritional composition and functional properties of different varieties of …

Evaluation Of Strip-Tillage And Banded Phosphorus And Potassium Fertilizer For Corn Production In Eastern South Dakota, Charles N. Tesch

Electronic Theses and Dissertations

In South Dakota, the adoption of no-till and other conservation tillage practices has grown in recent years. However, conventional tillage is still a common practice in the eastern third of the state, negatively affecting soil health and the environment. Strip-tillage has gained interest in this region as a way to transition to conservation tillage while retaining some benefits of conventional tillage. Strip-tillage also provides the ability to subsurface band nutrients, particularly phosphorus (P) and potassium (K) fertilizers within the tillage strip, potentially improving nutrient accessibility and recovery while reducing environmental risks. This study aimed to investigate (i) the effects of …

Investigating The Ruminal Metagenome Of Grass And Forage-Fed Bison To Uncover Metabolic Activities That Impact The Efficiency Of Plant Fiber Utilization, Anlly Fresno Rueda

Electronic Theses and Dissertations

This dissertation investigates the ruminal microbiome of American plains bison (Bison bison) to uncover the metabolic activities that enable efficient utilization of plant fiber, with a particular focus on bacterial composition and functional capabilities in response to forage quality. Using both 16S rRNA gene sequencing and metagenomic approaches, this research provides a comprehensive view of microbial diversity and metabolic potential in the rumen of bison, offering insights into their ecological resilience and efficiency in nutrient extraction. Chapter 2 focuses on the metabolic potential of bacterial communities in the rumen of bison fed a grass-based diet. Using high-throughput sequencing and metagenomic …

Identification Of Gut Bacterial Symbionts From Atlantic Salmon That Are Involved In Metabolizing Feed Ingredients Derived From Soybean Meal, Emily Fowler

Electronic Theses and Dissertations

In the aquaculture industry, plant-based protein sources have emerged as an economically and environmentally sustainable replacement for traditional animal protein sources. Yet, due to reduced digestibility, the presence of anti-nutritional factors, as well as an increased risk of inflammation of the digestive system, the inclusion of plant-based protein ingredients must be limited, especially in the diets of carnivorous fishes. To gain further insight on how to adapt the fish digestive tract to more effectively utilize or tolerate plant-based protein ingredients, slurry from cultured Atlantic Salmon (Salmo salar) was used as a proxy in an in vitro model system to identify …

Synchronization Protocols Utilizing Progesterone Implants Associated With Estrus Expression, Vaginal Microbiome, And Immune Response In Beef Cattle, Riley James Puck

Electronic Theses and Dissertations

This thesis aims to provide a deep understanding of presynchronization strategies and the varying factors affecting their efficacy. Beginning with a literature review of the estrous cycle and the endocrinology driving it. Building on this foundation, the physiological basis for estrous synchronization is described, followed by the advancement of pre-synchronization protocols designed to increase estrus and conception. Further, we will focus on the influence of estrus expression on insemination time and conception rates. Finally, how the immune system interacts with the conception process. In addition, the review goes beyond exogenous endocrine manipulation and dives into the emerging role of the …

One Rung At A Time: Climbing The Ladder Toward Aquatic Connectivity In Eastern South Dakota, Evan Spencer

Electronic Theses and Dissertations

Roads and streams are synonymous with their connection to movement, the acquisition of resources, and as pathways to new opportunities. However, the factors that define good roads and ecologically connected streams are rarely without conflict. Road infrastructure at stream-road crossings has the potential to fragment stream networks. Tube culverts, where streams pass under the road through metal pipes, are particularly concerning for stream connectivity. Undersized, aging, or inappropriately installed culverts can develop vertical drops at the outflow due to high velocities and stream bed scouring. The outlet drop can function as a barrier, preventing the upstream movement of fish. Due …

Determining The Susceptibility Status Of Red Sunflower Seed Weevil, Smicronyx Fulvus (Coleoptera: Curculionidae) To Pyrethroid Insecticides, Aaron Lee Hargens

Electronic Theses and Dissertations

Although sunflowers are native to North America, the early breeding efforts to improve sunflowers occurred outside of the continent. These hybrids were reintroduced to North America around 1875. In the early 1900’s, efforts to increase oil content were achieved by selective breeding of the reintroduced hybrids. Although this resulted in the development of improved sunflowers, many of the traits that conferred resistance to native insect pests were lost. One insect pest, the red sunflower seed weevil was noted as having caused sunflower production to rapidly decline and eventually end in Illinois and Missouri. Unfortunately, red sunflower seed weevils are still …

Quantification Of Auxin-Cytokinin Response During Lateral Root And Nodule Development In Medicago, Aniket Singh

Electronic Theses and Dissertations

Lateral roots (LRs) and nodules are post-embryonic root organs in legumes formed through the activity of de novo meristem. LRs, which are present in all vascular plants, are developed primarily via intrinsic developmental cues, whereas root nodules, found only in a small group of plants known as legumes, are formed in response to legume–rhizobium symbiosis. LRs arise from the pericycle layer of the root, whereas nodule initiation can begin either in the pericycle (indeterminate nodules with persistent meristem, as in Medicago) or in the cortex (determinate nodules with transient meristem, as in soybean), pointing toward a conserved developmental origin, especially …

Fungal Biotransformation Of Thin Stillage And Soybean Hulls And Bacterial Production Of Lysine In Ethanol Industry Co-Products, Bhawana Khadka

Electronic Theses and Dissertations

Thin stillage (TS) and whole stillage (WS) are major by-products of the corn ethanol industry. These by-products are generated in large quantities and contain nutrients that remain after yeast fermentation. Current industrial utilization of these by-products involves a series of downstream processing steps such as centrifugation and evaporation, which are energy-intensive and often result in nutrient losses. Traditionally, these by-products are dried to produce distillers dried grains with solubles. In this study, microbial fermentation was investigated as an alternative bioprocessing tool to expand the utilization of these by-products. The first objective was to explore the upcycling of TS, both alone …

Extended Days On Feed: Influences On Growth Performance, Efficiency, And Carcass Characteristics Of Steers And Heifers Of Different Proportions Of Angus And Limousin Genetics, Grace Henley Olinger

Electronic Theses and Dissertations

Recently, cattle feeders have extended days on feed (DOF) resulting in hot carcass weights (HCW) exceeding the limits of existing serial slaughter datasets. This study's goal was to determine the impact extended DOF exerts on growth performance, feed efficiency, and carcass characteristics of steers and heifers with varying proportions of Angus (AN) and Limousin (LM) genetics. In 2022, commercial AN cows from two MT ranches were artificially inseminated to 9 sires from 3 breeds (AN, LM, and Limflex (LF)) with parentage confirmed by DNA. In 2023, 216 calves (sired by 72 AN, 73 LF, and 71 LM; 105 steers and …

Characterization Of Major Foliar Fungal Species Affecting Cucurbits In South Dakota, Janani Perera Waduwarage Dona

Electronic Theses and Dissertations

Cucurbit crops comprise the largest area in vegetable production in South Dakota (USDA 2022). Information on the prevalence, distribution, and fungicide sensitivity of their fungal pathogens are unknown. This study characterized the major foliar fungal species affecting cucurbits in South Dakota through a 2-year, state-wide survey. Between 2023 and 2024, 789 leaves were recovered from pumpkin, cucumber, watermelon, squash, and bottle gourd. Alternaria spp. (70.72% of leaves) Fusarium spp. (6.84%), Podosphaera xanthii (4.18%), both Alternaria spp. and Fusarium spp. (3.42%), and both Alternaria spp. and Podosphaera xanthii (1.90%), Stagonosporopsis spp. (1.27%) and other genera of fungi (4.94%) were identified using …

Spatiotemporal Habitat Use, Sex-Specific Movement, And Site Fidelity Of Walleye In Lake Sharpe, Sd, Carter S. Dollen

Electronic Theses and Dissertations

Walleye Sander vitreus are a popular sportfish among anglers in South Dakota with large economic impacts to local communities. A popular destination for Walleye anglers is Lake Sharpe, a 25,000-ha reservoir on the Missouri River, in central South Dakota. Gill net catches in Lake Sharpe show high annual variation, prompting concern that gill net catch rates may be poorly related to population abundance. A better understanding of Walleye movement and distribution could lead to increased precision in annual abundance estimates and improved active management that increases angler success. The goals of this study were: 1) determine spatiotemporal movement patterns of …

Quantifying Avian And Vegetation Communities On Agricultural Conservation Easement Wetlands In Eastern South Dakota, Katherine Millman

Electronic Theses and Dissertations

The Prairie Pothole Region of eastern South Dakota contains numerous small depressional wetlands, colloquially known as potholes, that provide important ecosystem services, including water storage, water quality improvement, and wildlife habitat. The Agricultural Conservation Easement Program (ACEP) was established by the U.S. Department of Agriculture to protect and restore wetlands and grasslands on private land through easements such as Wetland Reserve Easements. Although ACEP requires management practices intended to maintain or improve habitat quality, few studies have evaluated their effectiveness at supporting wildlife communities and vegetation integrity. To address this gap, we evaluated wetlands at 30 ACEP sites and 20 …