Life Sciences Commons^™

Serological Survey And Pathogen Exposure Of Adult Female White-Tailed Deer In The Western Dakotas, Katherine L. Moratz, Bailey S. Gullikson, Eric S. Michel, Daniel M. Grove, Jonathan A. Jenks, William F. Jensen

Natural Resource Management Faculty Publications

Establishing baseline values for pathogen exposure and nutritional indices is necessary to monitor population health. However, little is known about white-tailed deer (Odocoileus virginianus) pathogen exposure and nutritional condition in the Northern Great Plains. Our objective was to assess pathogen exposure and establish nutritional indices for female white-tailed deer in Dunn and Grant counties, North Dakota and Perkins County, South Dakota. During 2014, we collected blood serum from 150 adult female white-tailed deer. Pathogens with the highest antibody prevalence included West Nile Virus (WNV; 85%), epizootic hemorrhagic disease (48%), and malignant catarrhal fever (32%). Serum values for creatine kinase, globulin, …

Looking To The Future: Key Points For Sustainable Management Of Northern Great Plains Grasslands, Lora B. Perkins, Marissa Ahlering, Diane L. Larson

Natural Resource Management Faculty Publications

The grasslands of the northern Great Plains (NGP) region of North America are considered endangered ecosystems and priority conservation areas yet have great ecological and economic importance. Grasslands in the NGP are no longer self-regulating adaptive systems. The challenges to these grasslands are widespread and serious (e.g. climate change, invasive species, fragmentation, altered disturbance regimes, and anthropogenic chemical loads). Because the challenges facing the region are dynamic, complex, and persistent, a paradigm shift in how we approach restoration and management of the grasslands in the NGP is imperative. The goal of this article is to highlight four key points for …

Identifying And Characterizing Salt-Tolerant Alfalfa (Medicago Sativa Subsp Falcata Germplasm, Katelin E. Frerichs, Lan Xu

Natural Resource Management Faculty Publications

Soil salinity limits plant growth and crop production. More than 20% of cultivated land worldwide is affected by salinity. The situation is becoming more severe due to shifts in precipitation and evaporation patterns and improper irrigation. There is an urgent need to develop salt-tolerant, economically valuable plants to minimize the loss of and to sustain agricultural production. Alfalfa is one of the most extensively cultivated forage crops. Some yellow-flowered alfalfa (Medicago sativa subsp. falcata) have exhibited morphological and physiological drought tolerance. Since soil salinity is associated with physiological drought, it is reasonable to expect that these drought tolerant falcata populations …

Isolation Of Potential Photosynthetic N2-Fixing Microbes From Topsoil Of Native Grasslands In South Dakota, Nanfang Wang, Shengni Tian, Liping Gu, Lan Xu, Yeyan Qiu, Trevor Van Den Top, Jose L. Gonzalez-Hernandez, Michael B. Hildreth, Shaoshan Li, Runbao Zhou

Natural Resource Management Faculty Publications

Nitrogen fertilizer is one of the most limiting factors and costly inputs in agriculture production. Current fossil fuel-dependent ammonia production is both energy intensive and environmentally damaging. An economically practical and environmentally friendly solution for the production of ammonia is urgently needed. Solar-powered N2-fixing cyanobacteria provide a unique opportunity and promise for applications in agriculture compared to all other N2-fixing bacteria that cannot use solar energy. Isolation of nitrogen-fixing microbes from the topsoil of native grasslands may have the potential to use them in crop fields as living ammonia factories. This may be a mechanism to free farmers from heavy …

Impacts Of Strategic Grazing And Fire On Soil Seed Bank Heterogeneity In Mixed-Grass Prairie, Kassidy Weathers, Lan Xu, Patricia Johnson

Natural Resource Management Faculty Publications

Native plant communities in the Northern Great Plains evolved under periodic fire and substantial grazing pressure from native herbivores, two main drivers maintaining the heterogeneity of grassland ecosystems. However, contemporary management practices focus on maximizing livestock production through fire suppression and uniform grazing strategies, resulting in decreased vegetation heterogeneity, species richness, wildlife habitat, and biodiversity. Objectives of this study were to evaluate and compare the effectiveness of patch-burn grazing (PBG) and winter-patch grazing (WPG) managements on soil seed bank heterogeneity in terms of species 1) richness, 2) composition, 3) abundance, and 4) diversity. Two soil cores (10-cm dia × 10-cm …

Weed Establishment And Persistence After Water Pipeline Installation And Reclamation In The Mixed Grass Prairie Of Western North Dakota, Erin K. Espeland, Lora B. Perkins

Natural Resource Management Faculty Publications

Weeds in reclamations interfere with success by: 1) competing with desirable species seeded during revegetation; 2) preventing recolonization of reclamations by native species; and 3) reducing the integrity of landscapes by expanding from reclamations into adjacent, intact areas. In the Bakken oilfield of western North Dakota, dispersed reclamation activity and increased traffic may provide many opportunities for weeds to spread. To determine the potential for disturbance and reclamation to increase resident weed populations and introduce new weed species, we tracked twenty-one weed (non-native/ruderal/invasive) species over a four-year period after the installation of a 1.8 km livestock water pipeline and subsequent …

Primers For Castilleja And Their Utility Across Orobanchaceae: I. Chloroplast Primers, Maribeth Latvis, Sebastian M. E. Mortimer, Diego F. Morales-Briones, Samuel Torpey, Simon Uribe-Convers, Sarah J. Jacobs, Sarah Mathews, David C. Tank

Natural Resource Management Faculty Publications

Premise of the study: Chloroplast primers were developed from genomic data for the taxonomically challenging genus Castilleja. We further tested the broader utility of these primers across Orobanchaceae, identifying a core set of chloroplast primers amplifying across the clade.
Methods and Results: Using a combination of three low-coverage Castilleja genomes and sequence data from 12 Castilleja plastomes, 76 primer combinations were specifically designed and tested for Castilleja. The primers targeted the most variable portions of the plastome and were validated for their applicability across the clade. Of these, 38 primer combinations were subsequently evaluated in silico and then validated across …

Development Of Highly Variable Microsatellite Markers For The Tetraploid Silene Stellata (Caryophyllaceae), Juannan Zhou, Michele R. Dudash, Charles B. Fenster, Elizabeth A. Zimmer

Natural Resource Management Faculty Publications

Premise of the study:We designed and tested microsatellite markers for the North American native species Silene stellata (Caryophyllaceae) to investigate its population genetic structure and identify selection on floral design through male reproductive success.

Methods and Results: A total of 153 candidate microsatellite loci were isolated based on next-generation sequencing. We identified 18 polymorphic microsatellite loci in three populations of S. stellata, with di- or trinucleotide repeats. Genotyping results showed the number of alleles per locus ranged from six to 45 and expected heterozygosity ranged from 0.511 to 0.951. Five of these loci were successfully amplified in S. …

Climate Change Impacts On Freshwater Wetland Hydrology And Vegetation Cover Cycling Along A Regional Aridity Gradient, Philip A. Fay, Glenn R. Guntenspergen, Jennifer H. Olker, W. Carter Johnson

Natural Resource Management Faculty Publications

Global mean temperature may increase up to 6°C by the end of this century and together with precipitation change may steepen regional aridity gradients. The hydrology, productivity, and ecosystem services from freshwater wetlands depend on their future water balance. We simulated the hydrology and vegetation dynamics of wetland complexes in the North American Prairie Pothole Region with the WETLANDSCAPE model. Simulations for 63 precipitation × temperature combinations spanning 6°C warming and −20% to +20% annual precipitation change at 19 locations along a mid-continental aridity gradient showed that aridity explained up to 99% of the variation in wetland stage and hydroperiod …

Consequences Of Hatch Phenology On Stages Of Fish Recruitment, David M. Bogner, Mark A. Kaemingk, Melissa R. Wuellner

Natural Resource Management Faculty Publications

Little is known about how hatch phenology (e.g., the start, peak, and duration of hatching) could influence subsequent recruitment of freshwater fishes into a population. We used two commonly sympatric fish species that exhibit different hatching phenologies to examine recruitment across multiple life stages. Nine yellow perch (Perca flavescens) and bluegill (Lepomis macrochirus) annual cohorts were sampled from 2004 through 2013 across larval, age-0, age-1, and age-2 life stages in a Nebraska (U.S.A.) Sandhill lake. Yellow perch hatched earlier in the season and displayed a more truncated hatch duration compared to bluegill. The timing of hatch influenced recruitment dynamics for …

Rapid Surface Water Volume Estimations In Beaver Ponds, Daniel J. Karran, Cherie J. Westbrook, Joseph M. Wheaton, Carol A. Johnston, Angela Bedard-Haughn

Natural Resource Management Faculty Publications

Beaver ponds are surface water features that are transient through space and time. Such qualities complicate the inclusion of beaver ponds in local and regional water balances, and in hydrological models, as reliable estimates of surface water storage are difficult to acquire without time and labour intensive topographic surveys. A simpler approach to overcome this challenge is needed, given the abundance of the beaver ponds in North America, Eurasia and southern South America. We investigated whether simple morphometric characteristics derived from readily available aerial imagery or quickly measured field attributes of beaver ponds can be used to approximate surface water …

Impacts Of Mowing Treatments On Smooth Bromegrass (Bromus Inermis) Belowground Bud Bank, L. Xu, J. Young, A. Boe, J. R. Hendrickson, N. H. Troelstrup Jr.

Natural Resource Management Faculty Publications

Introduced in the 1880s for improving forage production and controlling soil erosion, smooth bromegrass (Bromus inermis Leyss) has invaded and is threating numerous native prairie ecosystems and wildlife habitats in the Northern Great Plains. Land managers of the mixed-grass prairie ecosystems currently spend significant resources attempting to control invasive species and restore native grasslands with various management strategies including grazing, prescribed burning, herbicide application and seeding native species. Unfortunately, many studies have showed that such management efforts have minimal short-term effects. Without sustained effort, persistence and resurgence of smooth bromegrass is inevitable.

Research Update: Bud Bank Ecology For Understanding Perennial Grass Persistence, Lan Xu

Natural Resource Management Faculty Publications

Grassland ecosystems often demonstrate very remarkable resiliency to severe natural and anthropogenic disturbances. Such resiliency following disturbances comes from either seed banks (germinable seeds in the soil) or bud banks (meristems or buds, such as bulbs, bulbils, and buds on rhizomes, corms, and tubers, that generate vegetative tissues). Although seeds are important for dispersal, initial colonization, and maintenance of genetic diversity; few grass seeds persist in the soil more than five years, plus seed production often is unreliable under grazing. Recent studies have demonstrated that >99% of aboveground stems in undisturbed tallgrass prairie were recruited from the bud bank while <1% were recruited from the seed bank. Even under grazed or disturbed sites in tallgrass prairie, belowground buds make a significantly larger contribution (80%) to plant recruitment than do seeds (20%).

Greater Bud Outgrowth Of Bromus Inermis Than Pascopyrum Smithii Under Multiple Environmental Conditions, Jacqueline P. Ott, Jack L. Butler, Yuping Rong, Lan Xu

Natural Resource Management Faculty Publications

Tiller recruitment of perennial grasses in mixed-grass prairie primarily occurs from belowground buds. Environmental conditions, such as temperature, soil moisture and grazing can affect bud outgrowth of both invasive and native perennial grasses. Differential bud outgrowth responses of native and invasive species to climate change and grazing could alter competitive interactions that have implications for future land management. The aims of this work were to (i) compare how spring temperature altered bud outgrowth of native Pascopyrum smithii (Rydb.) Á. Löve (western wheatgrass) and introduced Bromus inermis Leyss.(smooth brome), (ii) compare how watering frequency altered bud outgrowth of these two species …

Invasive Grasses Consistently Create Similar Plant-Soil Feedback Types In Soils Collected From Geographically Distant Locations, Lora B. Perkins, Gary Hatfield, Erin K. Espeland

Natural Resource Management Faculty Publications

Aims Plants of similar life forms and closely related species have been observed to create similar types of plant–soil feedbacks (PSFs). However, investigations of the consistency of PSFs within species have not yielded clear results. For example, it has been reported that species create different types of PSFs in their native and introduced ranges. The aim of this project is to examine if four species create similar PSF types from soils collected from widely distributed areas within their introduced range. The soil for this project was collected from three areas in western North America. With this design, we aim to …

Assessing Temporal Genetic Variation In A Cougar Population: Influence Of Harvest And Neighboring Populations, Rebeca L. Juarez, Michael K. Schwartz, Kristine L. Pilgrim, Daniel J. Thompson, Stephanie A. Tucker, Joshua B. Smith, Jonathan A. Jenks

Natural Resource Management Faculty Publications

The geography of the Black Hills region of South Dakota and Wyoming may limit connectivity for many species. For species with large energetic demands and large home ranges or species at low densities this can create viability concerns. Carnivores in this region, such as cougars (Puma concolor), have the additive effect of natural and human-induced mortality; this may act to decrease long-term viability. In this study we set out to explore genetic diversity among cougar populations in the Black Hills and surrounding areas. Specifically, our objectives were to first compare genetic variation and effective number of breeders of …

Use Of Ecological Sites In Managing Wildlife And Livestock: An Example With Prairie Dogs, John R. Hendrickson, Patricia S. Johnson, Mark A. Liebig, Kevin K. Sedivec, Gary A. Halvorson

Natural Resource Management Faculty Publications

On the Ground

• The perception of prairie dogs among Native Americans living on the Standing Rock Sioux Reservation is mixed. Some Native Americans focus on the loss of forage productivity, whereas others are interested in the cultural and ecological aspects of prairie dogs.
• The use of ecological sites may provide a mechanism for developing a management framework that would consider both livestock and prairie dogs.
• The three ecological sites we surveyed had large differences in off-colony standing crop, but in 2 of the 3 years we surveyed, there were no differences between standing crop on-colony.
• This suggests that management of …

Effects Of Short-Term Cattle Exclusion On Plant Community Composition: Prairie Dog And Ecological Site Influences, Aaron Field, Kevin Sedivec, John Hendrickson, Patricia Johnson, Benjamin Geaumont, Lan Xu, Roger N. Gates, Ryan Limb

Natural Resource Management Faculty Publications

On the Ground

• Maintaining cattle and prairie dogs on rangelands is important ecologically, economically, and culturally. However, competition between these species, both actual and perceived, has led to conflict.
• We explored the effects of short-term (2-year) cattle exclusion on plant communities both on and off prairie dog towns and among three common ecological sites.
• Plant communities were different between on-town and off-town plots and among ecological sites but were similar between cattle-excluded and nonexcluded plots.
• Plant community composition did not differ between rangeland targeted for moderate forage utilization and that in which cattle had been excluded for 2 years.

Effect Of Grazing Prairie Dog—Colonized Rangeland On Cattle Nutrition And Performance: A Progress Report, Kenneth C. Olson, Christopher Schauer, Chanda Engel, Janna J. Kincheloe, Jameson R. Brennan, Ben L. Hauptman

Natural Resource Management Faculty Publications

On the Ground

• One objective of the ongoing Renewal on Standing Rock Reservation project is to evaluate the response of grazing steers to the level of prairie dog colonization on Northern Mixed Grass Prairie.
• We fenced four pastures to create an increasing gradient of a proportion of the pasture area colonized by prairie dogs. Pastures are stocked with yearling steers during each growing season.
• Comparing steer performance, Global Positioning System (GPS) locations of grazing, diet samples, and ingestive behavior at each proportion of the prairie dog colony per pasture allows prediction of the optimal proportion of colonization, which enables selection …

Seed Source May Determine Field-Specific Germination And Emergence: The Source By Planting Environment Interaction, Erin K. Espeland, Lora B. Perkins, Matthew E. Horning, Richard C. Johnson

Natural Resource Management Faculty Publications

Farm environmental conditions and management practices can result in within-cultivar differences in seed quality and lead to transgenerational plasticity (farm-specific effects on offspring, or TGP) that affect germination and emergence in transplant fields. We used three perennial bunchgrasses, [green needlegrass (Nassella viridula) ‘Lodorm’, slender wheatgrass (Elymus trachycaulus) ‘Pryor’, and bluebunch wheatgrass (Pseudoregneria spicata) prevariety registered germplasm P-7] to determine if seeds exhibited TGP. We also determined if TGP was affected by the interaction between production farms and planting environments (farm × environment interaction, or context-dependent TGP), using four laboratory temperature regimes to test germination response and four field environments to …

Winter Habitat Quality But Not Long- Distance Dispersal Influences Apparent Reproductive Success In A Migratory Bird, Clark S. Rushing, Peter P. Marra, Michele R. Dudash

Natural Resource Management Faculty Publications

Long- distance breeding and natal dispersal play central roles in many ecological and evolutionary processes, including gene flow, population dynamics, range expansion, and individual responses to fluctuating biotic and abiotic conditions. However, the relative contribution of long- distance dispersal to these processes depends on the ability of dispersing individuals to successfully reproduce in their new environment. Unfortunately, due to the difficulties associated with tracking dispersal in the field, relatively little is known about its reproductive consequences. Furthermore, because reproductive success is influenced by a variety of processes, disentangling the influence of each of these processes is critical to understanding the …

A Simple Method To Reduce Interpretation Error Of Ages Estimated From Otoliths, Bradley J. Smith, Daniel J. Dembkowski, Daniel A. James, Melissa R. Wuellner

Natural Resource Management Faculty Publications

We designed and tested a novel otolith viewing apparatus termed the otolith illumination device (OID) to ascertain if its use would result in a reduction of interpretation error as determined by increased precision of age estimates obtained from otoliths of walleye Sander vitreus and smallmouth bass Micropterus dolomieu. Clarity of annuli on otolith sections viewed with the OID was generally greater than clarity of annuli on sections viewed with an alternative method. OID-based age estimates were equally as, and in some instance more precise than ages estimated using the alternative method. Additionally, no systematic differences in coefficients of variation …

Validation Of A Technique For Estimating Alfalfa (Medicago Sativa) Biomass From Canopy Volume, Christopher G. Misar, Lan Xu, Arvid Boe, Roger N. Gates, Patricia S. Johnson

Natural Resource Management Faculty Publications

Determining biomass production of individual alfalfa (Medicago sativa L.) plants in space planted evaluation studies is generally not feasible. Clipping plants is time consuming, expensive, and often not possible if the plants are subjected to grazing. A regression function (Bʹ = 0.72558 + 0.11638 × Vʹ) was developed from spaced plants growing on rangeland in northwestern South Dakota near Buffalo to nondestructively estimate individual plant biomass (B) from canopy volume (V). However, external validation is necessary to effectively apply the model to other environments. In the summer of 2015, new data to validate the model were collected from spaced plants …

Influence Of Habitat And Intrinsic Characteristics On Survival Of Neonatal Pronghorn, Christopher N. Jacques, Jonathan A. Jenks, Troy W. Grovenburg, Robert Wayne Klaver

Natural Resource Management Faculty Publications

Increased understanding of the influence of habitat (e.g., composition, patch size) and intrinsic (e.g., age, birth mass) factors on survival of neonatal pronghorn (Antilocapra americana) is a prerequisite to successful management programs, particularly as they relate to population dynamics and the role of population models in adaptive species management. Nevertheless, few studies have presented empirical data quantifying the influence of habitat variables on survival of neonatal pronghorn. During 2002–2005, we captured and radiocollared 116 neonates across two sites in western South Dakota. We documented 31 deaths during our study, of which coyote (Canis latrans) predation (n = 15) was the …

The Importance Of Analyzing Neighbor Competitive Response In The Target–Neighbor Experimental Design, Kevin J. Berry, Michele R. Dudash

Natural Resource Management Faculty Publications

The role of competition in community structure and species interactions is universal. However, how one quantifies the outcome of competitive interactions is frequently debated. Here, we review the strengths and weaknesses of the target– neighbor design, a type of additive design where one of the competing species is reduced to a single individual and where controls and analyses are used for the target, but not for the neighbors. We conducted a literature review to determine how the target–neighbor design has been typically used and analyzed. We found that historically, targets were often smaller than neighbors and introduced after neighbor establishment; …

Buteo Nesting Ecology: Evaluating Nesting Of Swainson’S Hawks In The Northern Great Plains, Will M. Inselman, Shubham Datta, Jonathan Jenks, Kent Jensen, Troy Grovenburg

Natural Resource Management Faculty Publications

Swainson’s hawks (Buteo swainsoni) are long-distance migratory raptors that nest primarily in isolated trees located in areas of high grassland density. In recent years, anthropogenic conversion of grassland habitat has raised concerns about the status of the breeding population in the northern Great Plains. In 2013, we initiated a study to investigate the influence of extrinsic factors influencing Swainson’s hawk nesting ecology in north-central South Dakota and south-central North Dakota. Using ground and aerial surveys, we located and monitored nesting Swainson’s hawk pairs: 73 in 2013 and 120 in 2014. We documented 98 successful breeding attempts that fledged …

Early Weaning Reduces Rangeland Herbage Disappearance, Patricia Johnson, Kenneth C. Olson, Roger N. Gates, Hubert H. Patterson, Mindy Hubert, Douglas Landbloom, Janna J. Kincheloe, Heather A. Richter, Allison V. Grove

Natural Resource Management Faculty Publications

Early weaning of beef calves reduces nutrient and forage demand in a cow–calf enterprise, potentially contributing to reduction in forage utilization on the pasture from which calves are removed by a nonlactating cow vs. a cow–calf pair. Research was conducted to evaluate weaning beef calves 90 days early (EW) vs. normal weaning (NW) on pasture herbage disappearance in mixed-grass prairie pastures in the northern Great Plains. Spring-calving cows (n = 48) were utilized in each study year (2003, 2004, and 2006) from the date of early weaning (August) until the date of normal weaning (November). Cow–calf pairs were randomly …

Mate Replacement And Alloparental Care In Ferruginous Hawk, Shubham Datta, Will M. Inselman, Jonathan A. Jenks, Kent Jensen, Christopher C. Swanson, Robert W. Klaver, Indrani Sasmal, Troy W. Grovenburg

Natural Resource Management Faculty Publications

Alloparental care (i.e., care for unrelated offspring) has been documented in various avian species (Maxson 1978, Smith et al. 1996, Tella et al. 1997, Lislevand et al. 2001, Literak and Mraz 2011). A male replacement mate that encounters existing broods has options, which include alloparental care or infanticide. Infanticide may be beneficial in some species (Rohwer 1986, Kermott et al. 1990), but in long-lived avian species, like the ferruginous hawk (Buteo regalis) that do not renest within a season, infanticide might be detrimental. Adoption and rearing success likely provide direct evidence of competence of replacement mates as potential parents for …

A Comparative Study Of The Effects Of Non-Starch Polysaccharide Gums On Physical Properties Of Single-Screw Extruded Aquafeed, Michael Brown, Parisa Fallahi, Kasiviswanathan Muthukumarappan, Poonam Singha, Scott Sindelar

Natural Resource Management Faculty Publications

A factorial experimental design (5×3×2) was used to investigate the effects of non-starch polysaccharide binding agents on physical properties of single-screw extrusion. Extrusion cooking trials were performed with an ingredient blend for yellow perch, fortified with five non-starch polysaccharide binding agents including three plant-origin gums (guar, wheat gluten, carboxymethyl cellulose (CMC)) and two microbial-origin exopolysaccharide gums (xanthan and pullulan), with three levels of gum inclusion (3, 6, and 10%), and two levels of screw speed (100 and 150 rpm). Effects of the independent variables on extrudate characteristics were extensively analyzed and included density, expansion ratio, water absorption and solubility indices, …

West Nile Virus And Ferruginous Hawks (Buteo Regalis) In The Northern Great Plains, Shubham Datta, Jonathan A. Jenks, David Knudsen, Kent Jensen, Will M. Inselman, Christopher C. Swanson, Troy W. Grovenburg

Natural Resource Management Faculty Publications

Emerging infectious diseases (EID) present significant threats to the conservation of global biodiversity (Daszak et al. 2000). Evaluating impacts (spatial, temporal and demographic) of EIDs on sensitive and declining wildlife populations is challenging because quantitative information is usually dependent on estimates rather than counts (Wobeser 2007) and mortality rates are seldom quantified with conventional monitoring (Naugle et al. 2005).