Life Sciences Commons^™

The Effects Of Temperature And Phasins On Polyhydroxyalkanoic Acid (Pha) Accumulation In Heterologous Hosts Containing A Pha Synthase From Vibrio Species B-18, Edward R. Lyons

Master's Theses, Dissertations, Graduate Research and Major Papers Overview

Polyhydroxyalkanoic acids (PHA) are bacterial carbon reserves with application as biodegradable biocompatible plastics. PHA synthase, encoded by phaC, creates the PHA polymer. Evidence presented by many researchers has emphasized the role of phasin proteins in PHA accumulation. Phasins have been implicated in PHA granule localization, PHA synthase activation, and possibly chaperone and nucleoid binding functions. The phaC gene from Vibrio species B-18 with and without an upstream gene showing homology to phasin genes (orf2) were placed into runaway replication vectors, which use temperature to control gene expression. The aim of this thesis is to explore earlier observations that lower temperatures …

Expression And Purification Of N-Terminally Acetylated Microtubule Binding Protein Tau, Abigail Fleurima

Honors Projects

The microtubule-associated protein Tau (MAPT) plays a critical role in many neurodegenerative diseases. Tau functions to stabilize microtubule structures that are essential for transport within the neuron, and transport disruption leads to loss of neuronal function. Tau binding is regulated by phosphorylation with the help of kinases that add phosphate groups which block microtubule binding sites, and phosphatases that remove phosphate groups and expose the microtubule-binding regions. Tau can be found in hyper-phosphorylated states, which causes Tau to self-assemble into aggregates and prevent microtubule binding. It's clear that post-translational modifications (PTMs) of Tau play a key role in the dysregulation …

Function Of Spiny Dorsal Fin Erector Muscles In The Bluegill, Lepomis Macrochirus, Zakiyat Djabakatie

Honors Projects

Local motor control directly contributes to stability, which can be compromised by injury or multiple neuromuscular disorders. In addition, lack of sensory perception as experienced by decreased limb sensation can further deteriorate one‚Äôs quality of life. The goal of this study is to use bluegill (Lepomis macrochirus) fins as model systems to study and gain insights on local motor control and sensory perception to improve stability and locomotion in humans, especially in a rehabilitative state. We hypothesize that 1) when exposed to turbulence (T), bluegill will use the spiny dorsal fin to recover stability and muscle intensity and duration will …

Investigating The Role Of Alx4a In Mitfa Repression And Iridophore Fate Biasing, Melanie Cragan

Honors Projects

Investigating the role of the transcription factor alx4a on chromatophore fating in zebrafish.

What’S Water Got To Do With It? Place-Related Symbolic Meanings Alter Residents’ Perceived Effects Of Coastal Infrastructure, Bryce B. Dubois, Katherine Lacasse, Aaron J. Ley

Faculty Publications

The symbolic meanings residents associate with their local bodies of water and coastal infrastructure can shape their perceptions of the infrastructure’s effects. In this case, we conduct a survey (N = 168) to examine residents’ perceptions of visibly disruptive, yet environmentally

protective cooling towers attached to a long-standing coal-fired power plant on Mount Hope Bay. Residents’ symbolic meanings of the bay corresponded predominantly with aesthetic,

ecological, or recreational themes, whereas their symbolic meanings of the towers also focused on aesthetics as well as the towers’ function or uselessness. Although residents generally perceived the towers as having negative effects on …