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Multigeneration Effects Of Chronic Exposure To Polycyclic Aromatic Hydrocarbons In Gulf Killifish (Fundulus Grandis), Andrea Yammine
Multigeneration Effects Of Chronic Exposure To Polycyclic Aromatic Hydrocarbons In Gulf Killifish (Fundulus Grandis), Andrea Yammine
LSU Master's Theses
The goal of this study was to examine potential adaptation within two generations of Gulf killifish exposed to polycyclic aromatic hydrocarbons (PAH) while also investigating the use of mucus as an effective and non-lethal alternative to determine concentrations of 11-ketotestosterone (11-KT) and vitellogenin (VTG) of F. grandis. Multiple biomarkers were used to assess adaptation within these fish, such as lysozyme (enzyme critical in innate immunity), and 11-ketotestosterone and vitellogenin (endocrine hormones often affected by PAHs).
Mucal concentrations of 11-KT and VTG were determined and evaluated for effectiveness through comparative assays with blood plasma. This method was then applied to …