Life Sciences Commons^™

Reprograming Neuronal Cells By Overexpression Of Fibroblast-Specific Transcription Factors, Abdulmohsen Alanazi

Masters Theses

In mammals, a complex system of regulatory signals distinguishes tissues, structures and functions. Combinations of transcription factors and co-factors regulate activation and repression of genes that result in cellular differentiation. Whole genome arrays allow the monitoring of genomic expression in specific tissues. Fibroblast microarray studies have shown candidate genes that may be involved in fibroblast identification, including genes that express transcription factors Prrx1, Snai2 and Twist1. A previous study showed that the Prrx1 and Snai2 could reactivate a fibroblast phenotype in hybrid cells that had lost fibroblast identity. Furthermore, overexpression of these factors in liver-derived cells strongly repressed liver gene …

Nest Success In A Grassland Bird Community: Microsite Selection & A Case For Nest Height Manipulation, Matt Kneitel

Masters Theses

Grassland birds are a sensitive community and populations around the country have been declining. They are susceptible to low nest success due to loss of habitat and the small, fragmented patches that remain are often subjected to elevated levels of nest predation. The American goldfinch (Spinus tristis), which uses grasslands for feeding and nesting, is understudied given its abundance, due in part to its late-season nesting habits, and selection of taller woody vegetation as nesting substrate, which is atypical for many grassland nesting birds. American goldfinches were incorporated in this study which included nest height manipulation of goldfinch …

Seasonal Temperature Changes In Three Midwestern Wastewater Streams And The Effects On Fish Assemblages And Physiology, Ryan William Sparks

Masters Theses

Midwestern United States undergo extreme temperature and precipitation fluctuations throughout the year, which leads to changing habitats within Illinois river systems. Wastewater treatment facilities have long been used to treat sewage and reduce the organic products in sewage before releasing it into aquatic environments. Wastewater treatment facilities must maintain optimal conditions, especially temperature, for reproduction and growth of facilitated bacteria that break down organic materials in wastewater. The objectives for this study were to determine the thermal effects of wastewater treatment facilities on receiving waters; and secondly, to investigate the influence of these wastewater facilities on fish abundance and assemblages. …

Aging Techniques & Population Dynamics Of Blue Suckers (Cycleptus Elongatus) In The Lower Wabash River, Dakota S. Radford

Masters Theses

The Blue Sucker, Cycleptus elongatus, occurs in large rivers primarily in the Mississippi River basin. Their imperiled status has called attention to the need for its management and protection. Estimating age is crucial for directing management, but past studies have varied in their choice of hard structure, resulting in uncertainty regarding the basic life history of this species. Because the Wabash River Blue Sucker population may be one of few surveyable populations with high abundance and successful reproduction, their demographics can provide a benchmark against which threatened populations can be compared. We harvested Blue Suckers (n = 168) …

Seasonal Variation In Mitochondrial Bioenergetics Of The Bluegill Sunfish, Lepomis Macrochirus, From A Shallow Midwest River, Derick Isaac Lamptey

Masters Theses

As average global temperature increase, the frequency and magnitude of extreme temperatures in shallow aquatic ecosystems are more ubiquitous. In order to understand how these changing thermal regimes affect aquatic ectotherms, it is essential to develop studies evaluating the response of ectotherms to seasonal fluctuating thermal regimes. Previous studies on fluctuating temperature regimes have reported an increased physiological stress leading to morphological, behavioral and biochemical adaptations. From the latter, the adaptive capacity and seasonal performance associated with optimal function of the oxidative phosphorylation system (OXPHOS) are key for species persistence. However, studies on this matter are scarce. This study explores …

Assessment Of The Immune Response To Posthodiplostomum Minimum Infection In Bluegills, Olamide S. Olayinka

Masters Theses

Bluegills (Lepomis macrochirus) are common intermediate hosts for white grub (Posthodiplostomum minimum). They tolerate heavy infections with minimal effect on condition and continue to accumulate metacercariae as they age. This suggests that any immune response to this parasite might not be effective. This study was conducted to better understand the immune mechanisms underlying P. minimum infection in bluegills.

Infected organs (liver, kidney, and heart) were examined histologically, and serum from infected fish was tested for antibodies to white grub. Juvenile flukes were recovered from isolated metacercarial cysts. Polyclonal antibodies were produced in mice against white grub …

Monitoring Temporal Trends In Catostomidae Larvae In Large Sized River Tributaries, Kellie Nicole Hanser

Masters Theses

Large river ecosystems can support a complex network of tributaries that provide optimal habitat for fish reproduction. Anthropogenic alteration of rivers and tributaries has been shown to have adverse effects on river fish communities, but few studies have focused on the effects river regulation may have on the reproduction of native suckers (Family Catostomidae). In this study, I evaluated differences in biotic and abiotic factors associated with catostomid reproduction between tributaries of an altered and free-flowing river. I conducted ichthyoplankton sampling using drift nets and larval push nets in tributaries of the Illinois River, which is regulated for navigation, and …

Identification And Analysis Of Genes With Differential And Tissue-Specific Expression In Poplars, Bailey Mitchell

Masters Theses

Poplars have become one of the most frequently studied plant species for their applications in biotechnology, commercial benefits, and ecological importance. Microarray analysis is commonly used to analyze differential gene expression in many organisms. Investigations have examined differential gene expression and performed functional analyses on a single tissue or organ within poplar trees or a closely related set of tissues and organs, but usually do not broadly analyze gene expression across a large number of tissues and organs in poplar. This thesis work aims to generate data on the most differentially expressed genes for the major groups of tissues and …

Bigheaded Carp Spatial Reproductive Dynamics And Population Genetics In Large River Tributaries, Samuel J. Schaick

Masters Theses

Fisheries managers are devoting considerable effort, time, and funding towards limiting the spread of invasive Bighead and Silver (bigheaded) Carp. A better understanding of factors that influence spatial patterns of bigheaded carp reproduction can help fisheries managers prevent their spread. To examine factors that influence spatial patterns of reproduction, we sampled ichthyoplankton using drift nets and larval push nets in three tributaries of the Illinois and Wabash Rivers March-September 2016-2018. We compared relative abundances of bigheaded carp eggs and larvae among tributaries using catch per unit effort (CPUE). Abiotic data were analyzed from individual study tributaries and pooled among tributaries …

A Cloning Strategy For Expression Of Fungal Hemicellulases In Plant Systems, Amer Alrudayan

Masters Theses

Lignocellulosic biomass is notoriously difficult to deconstruct into lignin, hemicellulose, and cellulose fractions without the use of biochemical, chemical, and/or mechanical pretreatments. These pretreatment strategies add operational cost and in some cases produce hazardous waste that poses health and safety risks. Direct biological pretreatment from biomass-degrading bacteria and fungi may significantly reduce the costs and risks associated with these traditional strategies due to the innate ability of these organisms to modify plant cell walls. An additional measure that could further reduce the expenses associated with lignocellulosic biomass fractionation is genetic modification of the lignocellulosic feedstocks, which can be accomplished by …

The Transcriptome Response Of The White-Rot Fungus Trametes Versicolor To Wild-Type And Lignin-Modified Hybrid Poplar, Anbarah Alzabaidi

Masters Theses

Plant biomass is a renewable and sustainable feedstock for biofuel production that can reduce societal dependence on fossil fuels. However, the production of liquid biofuels from the non-starch (i.e. lignocellulosic) material through fermentation technology is limited due to the complexity of the cell wall structure. This necessitates the use of chemical, thermal, and/or mechanical pretreatment technologies, which adds significant capital, operational, and environmental costs. Biological pretreatment strategies have the potential to mitigate these expenses by harnessing the innate ability of specialized bacteria and fungi to deconstruct lignocellulose. White-rot fungi (e.g. Trametes versicolor) have been shown to be …

Effects Of Insect Opportunists On A Four-Level Trophobiotic System Involving Nectar-Producing Galls Of The Cynipid Wasp Disholcaspis Quercusmamma (Hymenoptera: Cynipidae), Stephanie L. Smith

Masters Theses

The induction of plant galls is considered an adaptive life history trait found in many insect groups. The formation of galls provides several advantages to the gall maker, such as enhanced nutrition, favorable microclimate, and protection from natural enemies, including parasitoids, inquilines, and predators. Order Hymenoptera has many gall-making species, belonging to the gall wasp family Cynipidae. As an extended phenotype of the gall makers, some galls exhibit very sophisticated adaptive mechanisms involving multilevel species interactions. In particular, the oak galls of the Cynipid species Disholcaspis quercusmamma, found in much of Illinois, produce a palatable, sugary nectar-like secretion, attracting …

2019 Celebration Of Scholarship, Creativity, And Engagement, Beth Heldebrandt, Todd Bruns, Seth Yeakel, Matthew Eifrid

Programs

Scholarship, creativity, innovation, and the many forms of collaborative engagement that shape faculty life and student learning are central to the mission of Eastern Illinois University. For that reason, our faculty, department chairs, college deans, the Provost, and the entire campus community celebrate the achievements and outstanding contributions of our colleagues and students to their disciplines and professions every year. And, as the following pages illustrate, faculty engaged in continuing scholarly and creative activities that have provided outstanding mentoring experiences for our undergraduate and graduate students.

In addition to recognizing faculty performances, shows, publications, grants and other professional efforts, the …

Research Methods In Kinesiology And Sports Studies, Scott Ronspies

Faculty Research and Creative Activity

An exposure to various types of research methods in related subject areas, a critical evaluation of selected studies, and writing the research report.

Reduced File Size: Quick And Easy Guide To Common Butterflies: An Introduction To The 25 Most Commonly Encountered Butterflies In The Midwest, Paul Switzer

Faculty Research & Creative Activity

If you are new to butterfly watching, this is the book for you! It covers 25 of the most commonly encountered butterflies in the Midwestern U.S. Learning these common species will give you a great start in your discovery of the more than 100 butterfly species in our region, many of which look very similar to those included in this guide.

Basketball Coaching, Scott Ronspies

Faculty Research and Creative Activity

The purpose of this course is to prepare the student/coach to effectively teach/coach the game of basketball to diverse levels of participants. This course provides the student with opportunities to gain knowledge and skill in coaching basketball and supports the concept of educating the whole individual in mental and physical domains. The principles of this course are based upon the scientific concepts from health/physical education & coaching.

Quick And Easy Guide To Common Butterflies: An Introduction To The 25 Most Commonly Encountered Butterflies In The Midwest, Paul Switzer

Faculty Research & Creative Activity

If you are new to butterfly watching, this is the book for you! It covers 25 of the most commonly encountered butterflies in the Midwestern U.S. Learning these common species will give you a great start in your discovery of the more than 100 butterfly species in our region, many of which look very similar to those included in this guide.

Quick And Easy Guide To Common Butterflies: An Introduction To The 25 Most Commonly Encountered Butterflies In The Midwest (Reduced File Size), Paul Switzer

Faculty Research & Creative Activity

If you are new to butterfly watching, this is the book for you! It covers 25 of the most commonly encountered butterflies in the Midwestern U.S. Learning these common species will give you a great start in your discovery of the more than 100 butterfly species in our region, many of which look very similar to those included in this guide.

Functional Characterization Of Glutamate Carboxypeptidase Ii In Caenorhabditis Elegans, Hashni Epa Vidana Gamage

Masters Theses

Glutamate carboxypeptidase II (GCPII) is a transmembrane zinc metalloprotease expressed in a number of organisms: from yeast to worm to humans. In humans, GCPII has been observed as a multifunctional protein and expressed in prostate, intestine, kidney, brain, tumor-associated neovasculature and other tissues as five paralogs. In the human small intestine, hGCPII is proposed to facilitate the folate absorption by cleaving terminal glutamate residues in dietary folates. Folates act as a cofactor in one-carbon metabolic pathways such as nucleotide synthesis, amino acid synthesis, DNA repair, and consequently involved in cell division and growth. The hGCPII homolog is found in the …

The Impact Of School Nutrition Education And Physical Education Programs On Weight Status Of Obese Children Aged 5-12 Years, Lamia Alotaibi

Masters Theses

Epidemiological studies indicate that 12.5 million children and adolescents are considered obese (Center for Disease Control and Prevention, 2017). Obesity is on the rise globally, and while it affects both children and adults, early measures can be taken to reduce the number of children who are obese, and therefore, come into adulthood at a higher risk for obesity-related diseases, such as hypertension, cardiovascular disease, and sleep apnea. There are many studies in which programs are implemented and tested for effectiveness; therefore, a systematic review of these studies that examine which factors play into overall obesity prevention and health improvements among …

Variation In Leaf Morphology Of An Alpine Shrub Community Along Environmental Gradients, Abdulssalam Khafsha

Masters Theses

Mountains represent complex environment gradients which strongly affect local temperature regimes and rainfall. These changes in climate conditions drive a diversity of plant responses. As alpine environments are expected to change dramatically in response to climate change, understanding plant responses to environmental variation is critical in these environments. Although they are a critical component of alpine communities, the environmental responses of shrubs have been much less examined than trees, representing an important knowledge gap. Leaves, as the primary photosynthetic organs on plants, would be expected to respond strongly to local climate conditions. In this study, I documented how the leaf …

White Grub In Bluegill In The Sangamon River: Impact Of Sewage Effluent And Flow Regime On Infection Parameters, Miranda White

Masters Theses

White grub (Posthodiplostomum minimum) is a digenetic trematode that uses a piscivorous bird, a pulmonate snail, and a centrarchid fish to complete its life cycle. To assess the impact of impoundment and sewage effluent on white grub infection parameters, infections in bluegill (Lepomis macrochirus) were monitored in 3 reaches of the Sangamon River near Decatur, Illinois: Reach 1- free-flowing river upstream of Lake Decatur Dam, unimpacted by sewage effluent, Reach 2- impounded flow below Lake Decatur Dam, unimpacted by sewage effluent, and Reach 3- free-flowing river downstream of Sanitary District of Decatur, impacted by sewage effluent. Liver and kidney abundances …

Haplotype Diversity And Population Genetic Structure Of Antistrophus Gall Wasps Associated With Two Silphium Species And Implication For Host Mediated Speciation, Jiaxin Deng

Masters Theses

Gall making insects form a special feeding guild of phytophagous animals, and by manipulating host plant tissue differentiation, are able to avoid plant chemical defenses and thus have no need for counter defense mechanisms. Host plant selection is crucial to the evolution of these insects because successful gall formation is largely dependent on host plant ability to respond to stimuli. In Illinois and neighboring states, Antistrophus gall wasps associated with the rosin weed (S. integrifolium) and the cup plant (S. perfoliatum) are morphologically indistinguishable and thus have been treated as belonging to single species. However, the wasps from the host …

Waist Circumference And The Relation To Aerobic Exercise And Perception Of Illness In Adults With Type 2 Diabetes Mellitus, Bailey J. Huebner

Masters Theses

The purpose of this study was to observe the relationship between waist circumference (WC), amount of aerobic exercise performed weekly and overall Health Belief Model (HBM) score in adults with diagnosed type 2 diabetes mellitus (T2DM). Members from a local Midwest adult fitness program (n = 5) who had been diagnosed T2DM participated in this study. Participants ranged in age from 70 to 80 years with an average age of 74 years. Four of the participants were female and one participant was male. The questions included in this study were 1 .) in those with diagnosed T2DM, was a low …

Creg1: Its Role As A Master Regulator Of Liver Function, Iffat Jahan

Masters Theses

Mammalian development involves a complex system of regulatory signals and reactions resulting in highly differentiated cell types with specific structure and function. The liver is a major organ that has been studied extensively to understand underlying genetic processes responsible for specification, establishment and maintenance of tissue identity. Hepatoma and hepatoma variant cell lines have been used as models to understand genetic networks responsible for liver function. Whole genome microarray analysis of hepatocyte cell lines has revealed candidate genes that may serve as regulators or master regulators of liver specificity. In two previous studies in our lab, the role of candidate …

Uridine Promotes Neurite Outgrowth In Neuro2a Cells, Jacquelyn Spathies

Undergraduate Honors Theses

Neurodegenerative diseases such as Alzheimer's and Parkinson's are the main causes of age-related dementia. These diseases can be due to neuronal cell death and/or impairment of neurite outgrowth. Giant oyster mushroom (GOM), Pleurotus giganteus, is used as a nootropic to improve cognitive function. GOM can also be used to prevent the onset of dementia. The underlying mechanism behind the medicinal property of GOM is unclear. Previous studies have shown that GOM has a high concentration of uridine. In this study, I examined the effects of uridine on neurite outgrowth in the Neuro-2a (N2a) neuroblastoma cell line. We also examined …

Genomic Dysregulation By Overexpression Of Transcription Factors, Mackenzie Scroggins

Undergraduate Honors Theses

As one of the major organs, the liver plays vital roles in the homeostasis of an individual. Being able to identify master regulator genes, genes whose product can affect the activation or deactivation of other genes, will enrich our understanding of hepatic function and liver disease. Previously, our lab used genome-wide microarray data to identify several transcription factors that may play a key role in liver expression. Here we ask whether overexpression of these transcription factors in a non-liver cell would result in liver phenotype activation or general disruption of gene regulation. To this end, we stably introduced seven transcription …

Exploration Of Sonic Hedgehog Gene Expression In Fathead Minnows (Pimephales Promelas), Brooke Renee Greiner

Undergraduate Honors Theses

Pollutants, as a result of wastewater treatments, have been shown to have negative impacts on aquatic ecosystems. To better understand the possible consequences caused by effluents on ecosystems, it is important to examine ecotoxicology data. One of the most commonly used species for water quality testing is the fathead minnow, Pimephales promelas. Ecotoxicology can then be taken one step further to understand the effects of pollutants on a molecular level. Previous research had identified effluents as causes for abnormal minnow fin morphology. In order to collect additional data on development, tanks with fathead minnows were placed at the Charleston …

Reprogramming Of Rat Hepatoma Cells With Fibroblast-Specific Protein Twist1, Kezban Ucar Cifci

Masters Theses

In mammalian development, a complex system comprised of regulatory signals causes tissue distinction with unique structures as well as functions. Gene expression controls these functions through specific combinations of transcription factors and cofactors that influence cell differentiation by both activation and repression of genes.

Whole genome microarray studies of fibroblasts have identified candidate genes that can serve as master regulators of fibroblast identity. A previous study showed that Prrx1 and Snai2 play important roles in activating expression of fibroblast identity, and Snai2 overexpression in hepatoma cells (Fg14) activated expression of fibroblast specific genes. Moreover, Snai2 overexpression resulted in repression of …

Investigating The Prevalence Of An Insecticide Resistance Gene In Culex Mosquito Populations Of Central Illinois, Kylee R. Noel

Masters Theses

The globalization of society has led to the spread of the vectors, mosquitoes, and diseases. Each year millions of people are killed by diseases vectored by mosquitoes. For example, West Nile Virus alone killed over 2,000 people in North America in 2017; 8 of those deaths being in Illinois. Two prominent vectors of West Nile Virus are Culex pipiens and Culex restuans; which are difficult to differentiate based upon morphological features. In this study, mosquitoes collected from two Illinois counties, Coles County and Champaign County, were first identified based upon morphology and this identification was then compared to probe-based …