Life Sciences Commons^™

How Do Animal Mitochondria Tolerate Water Stress?, Michael A. Menze, Steven C. Hand

Faculty Research & Creative Activity

The vast majority of animal species do not tolerate severe water stress, but the encysted embryo of the brine shrimp Artemia franciscana is an exceptionally useful organism to investigate physiological mechanisms for enduring extreme environmental insults. Any substantial reduction in cellular water poses a threat to survival. Nevertheless anhydrobiotic animals survive virtually complete loss of cellular water. The mechanisms that govern “life without water” (anhydrobiosis) are still not well understood. With certain exceptions, it seems that a recurring strategy for tolerating severe water loss involves the accumulation of both low molecular weight solutes (e.g. trehalose or other polyol) and highly …

Effect Of Rest Interval Length On The Volume Completed During Upper Body Resistance Exercise, Humberto Miranda, Roberto Simão, Leonardo Marmo Moreira, Renato Aparecido De Souza, João Antônio Alves De Souza, Belmiro Freitas De Salles, Jeffrey Willardson

Faculty Research and Creative Activity

The purpose of the current study was to compare the workout volume (sets x resistance x repetitions per set) completed during two upper body resistance exercise sessions that incorporated 1 minute versus 3 minute rest intervals between sets and exercises. Twelve trained men completed two experimental sessions that consisted of 5 upper body exercises (i.e. barbell bench press, incline barbell bench press, pec deck flye, barbell lying triceps extension, triceps pushdown) performed for three sets with an 8- RM load. The two experimental sessions differed only in the length of the rest interval between sets and exercises; one session with …

Investigation Of Insect Visits To Flowers Of Stylisma Pickeringii (Patterson Bindweed), An Endangered Plant Of Illinois Sand Prairies, A. E. Claerbout, H. R. Owen, Janice M. Coons, B. L. Todd

Faculty Research & Creative Activity

Little is known about the reproductive biology of Stylisma pickeringii var. pattersonii, an endangered plant in Illinois occurring in only three counties (Cass, Henderson, and Mason). The objective of this study was to estimate flower densities and temporal (time of day and seasonal) differences in insect visitation to flowers of S. pickeringii in 2002 for native populations in Henderson and Mason Counties. A 50 m transect was located where S. pickeringii was most abundant and quadrats (0.25 m2) were placed on alternate sides of the tape measure at every meter at random distances from the transect. The number of flowers …

Behavioral Explanations Underlying The Lack Of Trap Effectiveness For Small-Scale Management Of Japanese Beetles (Coleoptera: Scarabaeidae), Paul V. Switzer, Patrick C. Enstrom, Carissa A. Schoenick

Faculty Research & Creative Activity

Traps containing a combination floral and synthetic pheromone lure are used to monitor and manage Japanese beetles, Popillia japonica Newman (Coleoptera: Scarabaeidae). One key factor limiting trap effectiveness for beetle control is the "trap spillover" phenomenon, in which the trap attracts beetles without capturing them, resulting in increased damage to surrounding host plants. We investigated the mechanisms underlying trap spillover by conducting two studies in a soybean field in east central Illinois. In the first study, we set up trap stations for 1 d and compared the sex, size, and egg load (for females) of beetles caught in the traps …

Sucrose Phosphate Synthase Expression Influences Poplar Phenology, Thomas Canam, Ji-Young Park, Kyu-Young Kang, Faride Unda, Shawn D. Mansfield

Faculty Research & Creative Activity

The objective of this study was to manipulate the intracellular pools of sucrose, and investigate its role in regulating plant growth, phenology (leaf senescence and bud break) and fibre development. This objective was achieved by differentially expressing an Arabidopsis (Arabidopsis thaliana L. Heynh.) sucrose phosphate synthase (SPS) gene in hybrid poplar (Populus alba · Populus grandidentata Michx.), a model system for tree biology with substantial industrial relevance in the context of short rotation forestry and a target bioenergy crop. Phenotypic differences were evident in the transgenic trees, as both the timing of bud flush and leaf senescence were altered compared …

Investigation Of Insect Visits To Flowers Of Stylisma Pickeringii (Patterson Bindweed), An Endangered Plant Of Illinois Sand Prairies, A. Claerbout, H. Owen, Janice Coons, B. Todd

Faculty Research & Creative Activity

Little is known about the reproductive biology of Stylisma pickeringii var. pattersonii, an endangered plant in Illinois occurring in only three counties (Cass, Henderson, and Mason). The objective of this study was to estimate flower densities and temporal (time of day and seasonal) differences in insect visitation to flowers of S. pickeringii in 2002 for native populations in Henderson and Mason Counties. A 50 m transect was located where S. pickeringii was most abundant and quadrats (0.25 m2) were placed on alternate sides of the tape measure at every meter at random distances from the transect. The number of flowers …

Vegetative Anatomy And Systematics Of Triphorinae (Orchidaceae), Barbara Carlsward, William Stern

Faculty Research & Creative Activity

Triphorinae represents a group of three anatomically simple genera, the structural features of which are unspecialized. The anomocytic stomatal pattern occurs in all genera; it predominates in Triphora . A foliar hypodermis, sclerenchyma, fibre bundles and stegmata are absent. The mesophyll is homogeneous. The exodermal and endodermal cells in the roots are entirely thin-walled and tilosomes are absent. However, there are anatomical modifications that appear to be unique: root hairs in Monophyllorchis are borne on velamenal buttresses and, in Psilochilus , they arise endogenously. In the root vascular system of Psilochilus , the metaxylem occurs as a circumferential band. The …

Evaluation Of Glyphosate-Tolerant Soybean Cultivars For Resistance To Bacterial Pustule, Lopa Goradia, Glen Hartman, Steven Daniel

Faculty Research & Creative Activity

Xanthomonas axonopodis pv. glycines causes bacterial pustule of soybean, which is a common disease in many soybean-growing areas of the world and is controlled by a single recessive gene (rxp gene) commonly found in many conventional glyphosate-sensitive soybean cultivars. Since glyphosate-tolerant cultivars are commonly planted today, there has been no information about whether these new cultivars have bacterial pustule resistance. The goal of this study was to screen glyphosate-tolerant soybean cultivars for resistance to X. axonopodis pv. glycines. Three experiments were completed to evaluate resistance. In experiment 1, 525 commercial glyphosate-tolerant cultivars from 2001 were inoculated with X. axonopodis pv. …

Behavioral Explanations Underlying The Lack Of Trap Effectiveness For Small-Scale Management Of Japanese Beetles (Coleoptera: Scarabaeidae), Paul Switzer, Patrick Enstrom, Carissa Schoenick

Faculty Research & Creative Activity

Traps containing a combination floral and synthetic pheromone lure are used to monitor and manage Japanese beetles, Popillia japonica Newman (Coleoptera: Scarabaeidae). One key factor limiting trap effectiveness for beetle control is the "trap spillover" phenomenon, in which the trap attracts beetles without capturing them, resulting in increased damage to surrounding host plants. We investigated the mechanisms underlying trap spillover by conducting two studies in a soybean field in east central Illinois. In the first study, we set up trap stations for 1 d and compared the sex, size, and egg load (for females) of beetles caught in the traps …

The Effect Of Coalmine Effluent On The Parasite Assemblages Of Lepomis Spp. In The Saline River Basin, Andrew Claxton

Masters Theses

To determine the efficacy of using parasite assemblages as bioindicators of coalmine effluent, a survey of gastro-intestinal helminths from the gut of sunfish, Lepomis spp, was performed. Five species of Lepomis, L. cyanellus, L. nwcrochirus, L. megalotis, L. microlophus, and L. gulosus, were collected from six locations near the Palzo mine, an abandoned coalmine that actively discharges into the lower reach of Sugar Creek. Sunfish were collected from three sites which did not receive effluent from the Palzo mine (Sugar Creek (37° 37'/ 88° 50'), South Fork(37° 41 '/ 88° 52'), and the Little Saline River (37° 38'/ 88° 38')) …

Feathers As Bioindicators Of Pcb Exposure In Clapper Rails, Jay W. Summers

Masters Theses

In this study we used feathers as a biomonitor for exposure to the polychlorinated biphenyl (PCB) Aroclor 1268. Clapper rails have been used as an indicator species of environmental damage for the LCP superfund site located in Brunswick, GA USA. LCP is contaminated with Aroclor 1268, which has been used in limited amounts elsewhere and therefore can be used as a contaminant marker. Aroclor 1268, including congener profiles, were quantified using gas chromatography (GC). Concurrently each sample was quantified for total Aroclor 1268 using an enzyme-linked immunosorbant assay (ELISA) and compared to the GC results to determine if ELISA was …

Behavioral Response Of Japanese Beetles (Popillia Japonica) To Sex Pheromone: Exploring Factors Of Social Situation And Recent Mating Experience, Carissa A. Schoenick

Masters Theses

Insects in have several methods of advertising for mates; one important and common method is via the use of chemical pheromones. Insects release pheromones into the environment to communicate information about their sex, mating status and receptivity to others. For example, Japanese beetles (Popillia japonica) produce a very volatile sex pheromone conspecifics can readily detect. This pheromone is released by virgin females only, and triggers scramble competition among the males that detect and follow the signal for access to these females. Because Japanese beetles are an invasive species and an important pest of ornamental and agricultural plants, there is strong …

Photopigments As Descriptors Of Phytoplankton Assemblages For Biotic Assessment Of Illinois Lakes And Reservoirs: An Hplc Aided Analysis, Robert J. Krenz Iii

Masters Theses

The Illinois Environmental Protection Agency (IEPA) and U.S. Environmental Protection Agency (USEP A) identify needs to monitor aquatic resources by quantitative means using biocriteria in conjunction with routine environmental assessment to identify sources of impairment, and determine the effectiveness of management protocols (IEP A 2007; USEPA 2007). Though bioassessment protocols have been implemented within lotic systems and lakes of natural origin, investigation of their application to functionally unique reservoir habitat, which dominates the Midwest, has been limited. This study .evaluated the utility of photopigment profiles, attained using high-performance liquid chromatography (HPLC), for development ofbioassessment protocols for Illinois lakes and reservoirs. …

Dynamics Of Aggregation Formation In Japanese Beetles, Popillia Japonica, Katelyn A. Kowles

Masters Theses

For most insect aggregations to form, they need to be started by an initial individual (the pioneer) andjoined by later individuals (the joiners). Pioneers and joiners may differ with regard to characteristics such as sex and body size. We carried out three field experiments to examine the characteristics of Japanese beetles, Popillia japonica, pioneering and joining aggregations on host plants. Individual beetles were captured as they arrived on uninhabited grape plants, as well as plants designed to simulate aggregations with model beetles and feeding damage. For all experiments and treatments, the beetles arriving were significantly female-biased, with pioneer females having …

Ecology And Impacts Of Lianas In Regenerating Forests, Laura Marie Ladwig

Masters Theses

Although they are important components of forest communities, the general ecology of temperate lianas, especially during forest regeneration, is largely unknown. The dependence of lianas on other plants for physical support makes them a potentially important driver of community dynamics. I examined current and past vegetation data from an old-field succession study in the Piedmont region of New Jersey, USA to determine the dynamics of liana expansion and their role in forest regeneration. Fifty years of vegetation data were utilized to determine community controls and population dynamics oflianas during expansion. Five lianas, Celastrus orbiculatus, Lonicera japonica, Parthenocissus quinquefolia, Toxicodendron radicans, …

Aging In The Mammalian Olfactory System, Stephen Clark

Masters Theses

The purpose of this investigation was to investigate genetic and age~related differences in the mammalian olfactory system. The molecule of interest is apolipoprotein E (apoE), a component oflipoprotein molecules which functions in the transport of cell membrane components (triglycerides, lipids, and cholesterol). Specifically in the nervous system, apoE is synthesized by glial cells that function in recycling dead nervous tissue components for delivery to growing or dividing neurons. ApoE is present in three protein isofon11s: apoE2, which has been associated with type III hyperlipoproteinemia; apoE3, rhe normal human isoforrn; and apoE4, which has been associated with Alzheimer's disease (AD). The …

Interaction Of Apoe With Estrogen In The Olfactory System During Nerve Maintenance And Recovery, Michael M. Tonsor Ii

Masters Theses

Estrogen replacement profoundly aids in nerve regeneration after injury in adult mammalian models. Estrogen replacement also protects against olfactory dysfunction in mammals. The mechanism underly1ng estrogen's neuroprotective effects remains ambiguous. My goal is to investigate and better clarify if apolipoprotein E (apoE ), a lipid transporting protein, is a critical intermediary to estrogen's effects on nerve maintenance and regeneration.

My hypothesis is that apoE is a mediator of estrogen's beneficial effects. This hypothesis is based on the following observations from previous studies. First, olfactory nerve regeneration was delayed in apoE-gene knockout (KO) mice as compared to wild type (WT) mice. …

Fragmentation Effects On Fitness In Five Common Prairie Species, Lydia Miramontes Loyd

Masters Theses

Midwest tallgrass prairies have suffered extensive habitat loss and as a result persist as isolated and highly fragmented remnants. In such small, isolated remnants, genetic drift and inbreeding depression can lead to a loss of genetic variation over time. Reduced genetic variation is often associated with reduced levels of growth, survival, and reproduction as well as increased susceptibility to stress. This study examined fitness traits of five common prairie species to determine whether plants in small fragmented remnants had reduced fitness as compared to those in a larger remnant. Five prairie species were chosen for this study based on their …

Nest Success, Nesting Habitat, & Nestling Feeding Rates Of Red-Headed Woodpeckers In East-Central Illinois, Nathan Cole Hudson

Masters Theses

The Red-headed Woodpecker (Melanerpes erythrocephalus) is a classic oak savanna species that has suffered significant population declines caused, in part, by extensive loss of savanna habitat and agricultural development. Despite consistent data that reveal a dramatic decline throughout much of their range, few studies have examined other possible explanations. In an effort to determine potential causes for their continued decline (as well as add to existing knowledge of nesting biology), we examined nest success and nesting micro-habitat features. We compared micro-habitat variables at: 1) golf and non-golf course sites, 2) successful and failed nest sites, and 3) nest and random …

Analysis Of Transcription Factor Promoter Binding In Rat Hepatoma/Fibroblast Hybrids, Jordan C. Angle

Masters Theses

When hepatomas (liver cancer cells) are fused with fibroblasts (undifferentiated body cells), there is complete extinguishing of all tissue-specific gene expression. Once it was discovered that many hybrid cell lines do not express needed transcription factors for liver-specific gene transcription, the factors were reintroduced and forced to express through the use of plasmids. Even if the two primary transcription factors were present in the system, downstream tissue-specific gene expression was not rescued. To examine potential mechanisms for the repression of liver-specific gene expression in hepatoma x fibroblast hybrid cells, chromatin immunoprecipitation (ChlP) was used. This procedure allows the liver-specific gene …

Long-Term Assessment Of Predatory Fish Removal On A Pond-Breeding Amphibian Community In Central Illinois, Lee M. Gross

Masters Theses

Habitat loss and fragmentation are important contributors to population declines in many species, so maintaining suitable habitat has become a priority for wildlife management. Removal of introduced fish can help restore suitable habitats for amphibian populations, although long-term assessments of such removals are lacking at the community level. In 2001, drift fences and pitfall traps were constructed around the majority of four ponds to monitor an amphibian community in a nature preserve in central Illinois. Rotenone™ was applied to ponds containing introduced fish in 2001, and again, in 2003. I collected data from 2005-2007 to determine the long-term impacts of …

Habitat Selection And Niche Partitioning Of Fox And (Sciurus Niger) And Gray (Sciurus Carolinensis) Squirrels In Charleston, Il, Katelyn Marti

Masters Theses

Two species of tree squirrels inhabit Charleston, IL; fox squirrels (Sciurus niger) and gray squirrels (Sciurus caro/inensis). Although these species are sympatric throughout much of the eastern U.S., urban settings are usually occupied by one or the other. In the few localities where they co-occur, little is known about how they partition habitat. Therefore, the objectives of my study were to determine: (1) the relative abundance of each species, (2) whether habitat use differs between species, (3) if habitat use shifts with season, time of day, domestic predator abundance, or mast availability, and (3) observe interspecific and intraspecific interactions. The …

Survival Strategies Of The Endangered Physaria Ludoviciana (Silvery Bladderpod; Brassicaceae), Marissa Catherine Jernegan Grant

Masters Theses

Physaria ludoviciana (Nuttal) O'Kane and Al-Shehbaz is an endangered species in Illinois and Minnesota sand prairies, but has many individual populations in western states. Areas where P. ludoviciana occurs have high summer temperatures, a sandy soil with low water holding capacity and high drainage, the frequent disturbance of blowing sand and little canopy vegetation, giving it a full sun environment. This habitat is where few other plants are able to establish, providing P. ludoviciana with little competition. Physaria ludoviciana exists in a niche where it is adapted and able to establish and persist. The focus of this study was to …

Effect Of Surface Stability On Core Muscle Activity For Dynamic Resistance Exercises, Jeffrey Willardson, Fabio E. Fontana, Eadric Bressel

Faculty Research and Creative Activity

To compare core muscle activity during resistance exercises performed on stable ground vs. the BOSU Balance Trainer. Methods: Twelve trained men performed the back squat, dead lift, overhead press, and curl lifts. The activity of the rectus abdominis, external oblique abdominis, transversus abdominis/internal oblique abdominis, and erector spinae muscles was assessed. Subjects performed each lift under three separate conditions including standing on stable ground with 50% of a 1-RM, standing on a BOSU Balance Trainer with 50% of a 1-RM, and standing on stable ground with 75% of a 1-RM. Results: Significant differences were noted between the stable 75% of …

Ligustrum Vulgare L., Bret Hash

Specimens by Name

No abstract provided.

Fraxinus Lanceolata Borkh., John E. E. Ebinger

Specimens by Name

No abstract provided.

Stachytarpheta Jamaicensis Vahl, Richard J. Abbott

Specimens by Name

No abstract provided.

Saritaea Magnifica (Sprague Ex Stennis) Dugand, Richard J. Abbott

Specimens by Name

No abstract provided.

Parthenocissus Quinquefolia (L.) Planch., Paul B. Marcum, Loy R. Phillippe

Specimens by Name

No abstract provided.

Maclura Pomifera (Raf.) Schneid., Loy R. Phillippe, Paul B. Marcum

Specimens by Name

No abstract provided.