Life Sciences Commons^™

The Spatial Distribution Of The Japanese Beetle, Popillia Japonica, In Soybean Fields, Stacey A. Sara, Emily B. Mccallen, Paul V. Switzer

Faculty Research & Creative Activity

The Japanese beetle, Popillia japonica Newman (Coleoptera: Scarabaeidae), is a serious pest of many agricultural and horticultural plants. Relatively little research has investigated the distributions of Japanese beetles in agricultural fields, and this lack of information makes pest management more difficult. In the present study, the spatial distribution of Japanese beetles in soybean fields was examined. Specifically, how the distribution and abundance of beetles was affected by distance from an edge, edge direction, and edge type was examined. An edge effect for density was discovered; beetle numbers decreased significantly with increasing distance from the field edge. The east and south …

Assessing Multiple Endpoints Of Atrazine Ingestion On Gravid Northern Watersnakes (Nerodia Sipedon) And Their Offspring, Lorin A. Neuman-Lee, Karen F. Gaines, Kyle A. Baumgartner, Jayme R. Voorhees, James M. Novak, Stephen J. Mullin

Faculty Research & Creative Activity

Ecotoxicological studies that focus on a single endpoint might not accurately and completely represent the true ecological effects of a contaminant. Exposure to atrazine, a widely used herbicide, disrupts endocrine function and sexual development in amphibians, but studies involving live-bearing reptiles are lacking. This study tracks several effects of atrazine ingestion from female Northern Watersnakes (Nerodia sipedon) to their offspring exposed in utero. Twenty-five gravid N. sipedon were fed fish dosed with one of the four levels of atrazine (0, 2, 20, or 200 ppb) twice weekly for the entirety of their gestation period. Endpoints for the mothers included blood …

Exercise And Blood Flow Restriction, Zachary K. Pope, Jeffrey Willardson, Brad J. Schoenfeld

Faculty Research and Creative Activity

Pope, ZK, Willardson, JM, and Schoenfeld, BJ. Exercise and blood flow restriction. J Strength Cond Res 27(10): 2914– 2926, 2013—A growing body of research has demonstrated the effectiveness of exercise (low-intensity resistance training, walking, cycling) combined with blood flow restriction (BFR) for increased muscular strength and hypertrophy. The BFR is achieved via the application of external pressure over the proximal portion of the upper or lower extremities. The external pressure applied is sufficient to maintain arterial inflow while occluding venous outflow of blood distal to the occlusion site. With specific reference to low-intensity resistance training, the ability to significantly increase …

Examining The Causes Of Rarity For The Odonata Of Illinois, Miranda R. White, Paul V. Switzer

Faculty Research & Creative Activity

Odonata (dragonflies and damselflies) play an important role in habitat management and conservation, but our understanding of the causes of commonness versus rarity in this group is limited. In this study we examined the causes of rarity for the Odonata of Illinois. Using S-ratings for conservation status and published habitat classifications for Illinois odonates, we investigated whether habitat type (lotic versus lentic) or habitat specificity (whether they were limited to a specific type of aquatic habitat) was related to commonness. We found that lotic species and habitat specialists were more likely to be rare than lentic and generalist species. More …

A Spatially Explicit Model To Predict Radiocesium Body Burdens Of White- Tailed Deer On The U.S. Department Of Energy's Savannah River Site, Christopher W. Bobryk, Karen F. Gaines, James M. Novak, Susan A. Blas

Faculty Research & Creative Activity

We developed a spatially explicit exposure model to interpolate and predict radiocesium (137Cs) body burdens found in white-tailed deer (Odocoileus virginianus) on the U.S. Department of Energy’s (USDOE) Savannah River Site (SRS) to gain insight into and differentiate between the main contributing sources of this radionuclide for use in harvest management strategies that focus on minimizing human risk. Hunting has been allowed from permanent stands as a mechanism to manage the deer herd since 1965. All animals are monitored in the field for gross beta and gamma activity levels, providing a spatially explicit dataset. The models described here use the …

Isoform-Specific Effects Of Apoe On Neurite Outgrowth In Olfactory Epithelium Culture, Aseem Hussain, Minh Luong, Apryl Pooley, Britto P. Nathan

Faculty Research & Creative Activity

The apolipoprotein E4 (apoE4) genotype is a major risk factor for developing late-onset Alzheimer’s disease (AD). Inheritance of apoE4 is also associated with impairments in olfactory function in early stages of AD. In this project we examined the effects of the three common isoforms of human apoE (apoE2, apoE3, and apoE4) on neuronal differentiation and neurite outgrowth in explant cultures of mouse olfactory epithelium (OE).

The Reality Of Fantasy Sports: A Metaphysical And Ethical Analysis, Chad R. Carlson

Faculty Research and Creative Activity

Fantasy sports have become a major sector of our sport industry. With millions of participants worldwide and billions of dollars generated, fantasy sports have become a fixed part of our sport spectatorship. However, this prevalence has come without much intellectual investigation. Therefore, in this paper I discuss the metaphysics and ethics of fantasy sports. After providing arguments for the consistency of fantasy sports with prominent descriptions of play and games, I compare fantasy sports to other genres of play and games – sports, card games, ‘cybersports’, and spectatorship. After this juxtaposition, I delineate how fantasy sports are different from their …

Diapause And Anhydrobiosis In Embryos Of Artemia Franciscana: Metabolic Depression, Lea Proteins And Water Stress, Steven C. Handel, Yuvraj Patil, Shumin Li, Nilay Chakraborty, Apurva Borcar, Michael A. Menze, Leaf C. Boswell, Daniel Moore, Mehmet Toner

Faculty Research & Creative Activity

Metabolic depression is typically correlated with extended survival of environmental challenge and energy-limitation in early life stages of various invertebrates and vertebrates. Diapause is an ontogenetically-programmed reduction of development and often metabolism seen in many invertebrates. When embryos of Artemia franciscana enter the state of diapause, the overall metabolic depression is estimated to be greater than 99%. These embryos also contain trehalose and express multiple isoforms of Late Embryogenesis Abundant (LEA) proteins, constituents often present in a number of such anhydrobiotic animals. The mRNA levels for LEA proteins are highest in diapause and post-diapause embryos that possess desiccation tolerance, but …

Phenotypic And Genotypic Characterization Of Escherichia Coli Isolated From Untreated Surface Waters, Kai F. Hung, Steven L. Daniel, Kristopher J. Janezic, Blake Ferry, Eric W. Hendricks, Brian A. Janiga, Tiffany Johnson, Samantha Murphy, Morgan E. Roberts, Sarah M. Scott, Alexandra N. Theisen

Faculty Research & Creative Activity

A common member of the intestinal microbiota in humans and animals is Escherichia coli. Based on the presence of virulence factors, E. coli can be potentially pathogenic. The focus of this study was to isolate E. coli from untreated surface waters (37 sites) in Illinois and Missouri and determine phenotypic and genotypic diversity among isolates. Water samples positive for fecal coliforms based on the Colisure® test were streaked directly onto Eosin Methylene Blue (EMB) agar (37°C) or transferred to EC broth (44.5°C). EC broth cultures producing gas were then streaked onto EMB agar. Forty-five isolates were identified as E. coli …

Comparative Anatomy And Dorsal Fin Muscle Activity During Turning Maneuvers In Two Shark Species, Anabela Maia, Cheryl Wilga

Faculty Research & Creative Activity

Stability and procured instability characterize two opposing types of swimming, steady and maneuvering, respectively. Fins can be used to manipulate flow to adjust stability during swimming maneuvers either actively using muscle control or passively by structural control. The function of the dorsal fins during turning maneuvering in two shark species with different swimming modes is investigated here using musculoskeletal anatomy and muscle function. White-spotted bamboo sharks are a benthic species that inhabits complex reef habitats and thus have high requirements for maneuverability. Spiny dogfish occupy a variety of coastal and continental shelf habitats and spend relatively more time cruising in …

Isoform-Specific Effects Of Apoe On Neurite Outgrowth In Olfactory Epithelium Culture, Aseem Hussain, Minh Luong, Apryl Pooley, Britto Nathan

Faculty Research & Creative Activity

The apolipoprotein E4 (apoE4) genotype is a major risk factor for developing late-onset Alzheimer’s disease (AD). Inheritance of apoE4 is also associated with impairments in olfactory function in early stages of AD. In this project we examined the effects of the three common isoforms of human apoE (apoE2, apoE3, and apoE4) on neuronal differentiation and neurite outgrowth in explant cultures of mouse olfactory epithelium (OE).

Examining The Causes Of Rarity For The Odonata Of Illinois, Miranda White, Paul Switzer

Faculty Research & Creative Activity

Odonata (dragonflies and damselflies) play an important role in habitat management and conservation, but our understanding of the causes of commonness versus rarity in this group is limited. In this study we examined the causes of rarity for the Odonata of Illinois. Using S-ratings for conservation status and published habitat classifications for Illinois odonates, we investigated whether habitat type (lotic versus lentic) or habitat specificity (whether they were limited to a specific type of aquatic habitat) was related to commonness. We found that lotic species and habitat specialists were more likely to be rare than lentic and generalist species. More …

The Effect Of Accurate Categorical Activity Selection On The Prediction Of Percent Body Fat When Using The Omron Hbf-306 Bioelectric Impedance Analyzer, Abby Moulton

Masters Theses

Accurate methods for assessing body composition are important in evaluating health risk and fitness levels in individuals. Bioelectric Impedance Analysis has become a popular field method for assessing body composition because it is inexpensive, noninvasive, and easy to administer. The purpose of this study was to compare the activity level settings (Normal vs. Athlete) on the Omron HBF-306 bioelectrical impedance device to the criterion method, air displacement plethysmography, to evaluate the accuracy of the settings for college age females. It was hypothesized that the "Normal" setting would be more accurate for individuals described as normal by FIT index scores and …

Resolving The Nutritional Requirements Of The Bile Acid-Dehydroxylating Gut Bacterium Clostridium Scindens, Oindrila Paul

Masters Theses

Compared to the other parts of the human body, the gastrointestinal tract harbors a complex microbiota. The impact of the indigenous microbiota on host physiology is most pronounced in the colon, where the primary bile acids chenodeoxycholic acid and cholic acid are converted, via 7α-dehydroxylation, to the toxic secondary bile acids lithocholic acid and deoxycholic acid, respectively, by Clostridium scindens, an obligate anaerobe. Interestingly, other than bile acid dehydroxylation, little is known about the basic physiology of C. scindens. Understanding the metabolism of C. scindens will hopefully provide much-needed information as to how this gut bacterium impacts human health and …

Parasites Of Invasive Carp And Native Fish In The Wabash River, Justin Wilcox

Masters Theses

Although the majority of introduced non-native species fail to establish, proliferate and spread to the point of causing observable damage, there are a significant minority that achieve biological successes far beyond what they do in their native ranges. Parasites and pathogens are theorized to play an important role in determining the outcome of invasions by non-native species, both as potential mediators of the population growth of invasive species and as invasive species themselves. This study examines and compares the abundance and species richness of digestive tract helminths in native and non-native fish of the lower Wabash River, a single waterpool …

Dietary Ecology Of An Actively-Foraging Snake Species: Coluber Constrictor Foxii, Corissa Pauline Lennon

Masters Theses

The trophic niche width of a species varies depending on the foraging strategy employed by the individuals within a population. Among reptiles, the niche breadth of many species of snakes is relatively understudied. Within this clade, the genus Coluber includes wide-ranging, actively foraging snakes that have been historically labeled as dietary generalists. The dietary information on Blue Racers (Coluber constrictor foxii) is limited, and little is known about ontogenetic or seasonal differences in the prey species consumed. Additionally, this information is available from gut and fecal content only. In addition to obtaining gut and fecal contents, I employed stable isotope …

Morphometric Study Of Variation In The Genus Galeopsis (Lamiaceae), Michael T. Hughes

Masters Theses

The genus Galeopsis L. of the family Lamiaceae, also known as hemp-nettle, grows naturally in Europe and Asia, with four species naturalized in North America. The number of real species is in doubt, because of conflict over whether certain taxa are species, subspecies, or synonyms. The genus consists of five to eight taxa. I studied flower and leaf size ratios to clarify the distinctions of taxa in Galeopsis. These data were analyzed statistically, using cluster analysis, Principal Components Analysis (PCA), and Analysis of Variance (ANOVA). The results indicated that although some measurements, such as leaf width in G. angustifolia, …

Short-Term Responses Of Fish Assemblages To Habitat Restoration In A Small Midwestern Stream, John Leon West

Masters Theses

Recent efforts to restore stream habitat have become a common practice, yet evaluations of biotic responses to these practices are not as common. I evaluated fish assemblage response to restoration in a reach of Kickapoo Creek, a fourth order stream southwest of Charleston, Illinois. Restoration included 446 meters of bank stabilization, pool scouring keys, and the creation of two artificial riffles. To have a representation of pre-restoration fish assemblages, I sampled four stream reaches of Kickapoo Creek twice before construction of habitat restoration: two control reaches (upstream and downstream) and two restored reaches within a 0.5 km restoration stretch (upstream …

Freeze Tolerance Conferred By Transgenic Expression Of Late Embryogenesis Abundant Protein In Insect Cells, Alyssa N. Walser

Masters Theses

Anhydrobiotic organisms such as the brine shrimp Artemia franciscana have developed strategies to survive and thrive under conditions of limited water availability. One strategy is the accumulation of late embryo genesis abundant (LEA) proteins. Cells of Drosophila melanogaster (Kc167), which had previously been genetically modified to express a group 1 LEA protein (LEA1.3) found in A. franciscana, were used to test the impact of water stress due to freezing to -80°C. Two different freezing devices were used to determine the impact of the freezing protocol on the viability of cells after freezing and thawing. The first method used a 12-well …

Relationship Between Fundamental Movement Skills And Body Mass Index In Rural Elementary Age Children, Mariah L. Bolin

Masters Theses

A factor of how much physical activity a child gets could be determined by the level of mastery of fundamental movement skills that are needed for the establishment of skills used in many forms of adult physical activity. Children who master a number of fundamental movement skills are more likely to be physically active and they also may be more likely to take part in physical activity when being compared to peers with a lesser level of motor skill proficiency. Children who are considered overweight tend to engage less often in physical activities, which in turn may prevents them from …

Demographics Of A Commercially Exploited Population Of Flathead Catfish (Pylodictis Olivaris) In The Wabash River, Cassi Jo Moody-Carpenter

Masters Theses

Flathead catfish, Pylodictis olivaris, are a popular game and commercial fish in the Midwestern United States. In the lower 322 km of the Wabash River, flathead catfish are commercially and recreationally harvested by Illinois and Indiana fishers. Current management regulations are different between the two states; Illinois and Indiana have minimum length limits of 381 and 254 mm, respectively. Indiana has proposed changing their minimum length limit to 381 mm and allowing only one fish> 889 mm to be harvested per day. My study examined the current status of flathead catfish in the Wabash River to determine a standardized …

Eccentric Resistance Training And Blood Flow Restriction, Zachary Pope

Masters Theses

The purpose of this study was to compare the muscular strength and hypertrophic response following a four week training intervention involving resistance exercise utilizing only eccentric muscle contractions with, or without blood flow restriction (BFR), as well as a control group maintaining their current training regimen. Sixteen young male volunteers were recruited to participate in this study. Prior to the training intervention, subjects attended three sessions in order to obtain demographic information (age, height, body mass) and familiarize each of the participants with the testing and training equipment. After the familiarization session, muscular strength was assessed via elbow flexion one …

Determination Of Structural Stabilities Of Metal-Free Porphyrin Cytochrome C, Zinc Cytochrome C, And Cobalt Cytochrome C, Usha K. Tottempudi

Masters Theses

No abstract provided.

Effects Of Utilizing A Myofascial Foam Roll On Hamstring Flexibility, Elizabeth Sherer

Masters Theses

The purpose of this study was to identify if using a foam roller on the hamstring muscles would have positive effects and in tum increase hamstring flexibility. Hamstring injuries are prevalent in sports today. Lack of flexibility is seen as a possible cause for these injuries, so this study focused on a potential way of increasing hamstring flexibility. Self-induced myofascial release, using a foam roller, was the type of intervention used to try to have an effect on hamstring muscle flexibility. The use of a foam roller is becoming a popular tool utilized in many settings but there has been …

Cellular And Molecular Mechanisms Underlying The Interaction Of Apoe And Estrogen In Olfactory Neuronal Growth-Potential Relevance To Alzheimer's Disease, Minh Luong

Masters Theses

In recent years, there have been many studies looking at estrogen therapy (ET) as a treatment for Alzheimer's disease. Literature review suggests a close relationship between estrogen and apolipoprotein E (apoE) in the central nervous system. The mechanism underlying the interaction of apoE genotype with estrogen in the nervous system is not yet known. Therefore, the objectives of this research are 1) to determine the effects of estradiol treatment on basal cell proliferation and neuronal differentiation and 2) to identify the estrogen receptor subtype (ERα and ERβ) that mediates the increase of apoE levels, neurite outgrowth and neuronal numbers.

Results …

Survey Of Medicinal Herbs Of Central Illinois Prairie And Woodlands, Debra Ann Welch

Masters Theses

Illinois has been named the "Prairie State" for good reason. When the pioneers discovered a vast sea of grasses and forbs, they thought the prairie infertile. However, they soon found out that the blacksoil prairie was composed of a rich diversity of plant species that could provide much of the medical and nutritional needs of everyday life. The medicinal uses of many prairie species have not only been tested throughout history, but today modern science has also discovered their therapeutic importance.

The purpose of this study was to survey prairie species growing in the central Illinois counties of Coles and …

Molecular Mechanisms Involved In Anhydrobiosis Of Insect Cells, Diyagama Arachchi Ralalage Dilini Sewwandi Samarajeewa

Masters Theses

Animals possessing tolerance to extreme water stress are termed anhydrobiotes. Many desiccation tolerant organisms respond to water stress by intracellular accumulation of selected sugars such as trehalose and larger macromolecules such as Late Embryogenesis Abundant (LEA) proteins and thereby maintain the cell viability. Evidence indicates that the presence of trehalose and Late Embryogenesis Abundant (LEA) proteins may work synergistically to confer cellular protection during drying in eukaryotic cells. We evaluated any increase in cellular desiccation tolerance by expressing different LEA proteins in a non-desiccation tolerant insect cell line Drosophila melanogaster (fruit fly) (Kc167 cells) in the presence of trehalose.

Transgenic …

Late Embryogenesis Abundant Proteins Protect Human Hepatoma Cells During Acute Desiccation, Shumin Li, Nilay Chakraborty, Apurva Borcar, Michael A. Menze, Mehmet Toner, Steven C. Hand

Faculty Research & Creative Activity

Expression of late embryogenesis abundant (LEA) proteins is highly correlated with desiccation tolerance in anhydrobiotic animals, selected land plants, and bacteria. Genes encoding two LEA proteins, one localized to the cytoplasm/nucleus (AfrLEA2) and one targeted to mitochondria (AfrLEA3m), were stably transfected into human HepG2 cells. A trehalose transporterwas used for intracellular loading of this disaccharide. Cellswere rapidly and uniformly desiccated to low water content (<0.12 g H2O/g dry weight) with a recently developed spin-drying technique. Immediately on rehydration, control cells without LEA proteins or trehalose exhibited 0% membrane integrity, comparedwith 98% in cells loaded with trehalose and expressing AfrLEA2 or AfrLEA3m; surprisingly, AfrLEA3m without trehalose conferred 94% protection. Cell proliferation across 7 d showed an 18-fold increase for cells dried with AfrLEA3m and trehalose, compared with 27-fold for nondried controls. LEA proteins dramatically enhance desiccation tolerance in mammalian cells and offer the opportunity for engineering biostability in the dried state.

Metabolic Preconditioning Of Mammalian Cells: Mimetic Agents For Hypoxia Lack Fidelity In Promoting Phosphorylation Of Pyruvate Dehydrogenase, Apurva Borcar, Michael A. Menze, Mehmet Toner, Steven C. Hand

Faculty Research & Creative Activity

Induction of HIF-1α by oxygen limitation promotes increased phosphorylation and catalytic depression of mitochondrial pyruvate dehydrogenase (PDH) and an enhanced glycolytic poise in cells. Cobalt chloride and desferrioxamine are widely used as mimics for hypoxia because they increase the levels of HIF-1α. We evaluated the ability of these agents to elicit selected physiological responses to hypoxia as a means to metabolically precondition mammalian cells, but without the detrimental effects of hypoxia. We show that, while CoCl2 does increase HIF-1α in a dose-dependent manner, it unexpectedly and strikingly decreases PDH phosphorylation at E1α sites 1, 2, and 3 (Ser293, Ser300, and …

Intracellular Expression Of An Ice Nucleation Protein Reduces Cryoinjury In Insect Cells, Avril M. Harder

Student Honors Theses

Exposure of insect cells to subzero temperatures typically leads to cell membrane disruption and lethal intracellular ice formation. This study seeks to examine the cryoprotective value of trangenically expressing a bacterial ice nucleation protein (INP) in Spodoptera frugiperda (Sf-21) cells. The bacterium Pseudomonas syringae naturally produces a membrane-bound INP (inaZ), capable of structuring water and initiating ice formation at temperatures as high as -2 °C. I hypothesized that intracellular expression of an altered form of inaZ (PsINP) in Sf-21 cells will mediate highly regulated ice nucleation when cells are cooled to -80 °C in a slow, controlled manner, …