Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons™

Open Access. Powered by Scholars. Published by Universities.®

Eastern Illinois University

Discipline
Keyword
Publication Year
Publication
Publication Type
File Type

Articles 301 - 330 of 4790

Full-Text Articles in Life Sciences

Toxicodynamic Modeling Of 137cs To Estimate White-Tailed Deer Background Levels For The Department Of Energy's Savannah River Site, Karen F. Gaines, James M. Novak, Christopher W. Bobryk, Susan A. Blas Jan 2014

Toxicodynamic Modeling Of 137cs To Estimate White-Tailed Deer Background Levels For The Department Of Energy's Savannah River Site, Karen F. Gaines, James M. Novak, Christopher W. Bobryk, Susan A. Blas

Faculty Research & Creative Activity

The U.S. Department of Energy's (USDOE) Savannah River Site (SRS) is a former nuclear weapon material production and current research facility adjacent to the Savannah River in South Carolina, USA. The purpose of this study was to determine the background radiocesium (137Cs) body burden (e.g., from global fallout) for white-tailed deer (Odocoileus virginianus) inhabiting the SRS. To differentiate what the background burden is for the SRS versus 137Cs obtained from SRS nuclear activities, data were analyzed spatially, temporally and compared to other off-site hunting areas near the SRS. The specific objectives of this study were: to compare SRS and offsite …

Go to article

Effectiveness Of Hand Removal For Small-Scale Management Of Japanese Beetles (Coleoptera: Scarabaeidae), Paul V. Switzer, Ryan M. Cumming Jan 2014

Effectiveness Of Hand Removal For Small-Scale Management Of Japanese Beetles (Coleoptera: Scarabaeidae), Paul V. Switzer, Ryan M. Cumming

Faculty Research & Creative Activity

Hand removal is often recommended as a method for small-scale control of Japanese beetles (Popillia japonica Newman). In this study, we investigated the effectiveness of daily hand removal for controlling damage by Japanese beetles on grape plants. We also investigated whether the timing of the removal (at 0800, 1400, or 1900 hours, or at all 3 periods) inßuenced the effectiveness of the technique. We found that hand removal signiÞcantly lowered the number of beetles on, and consequently the damage to, grape plants relative to nonremoval controls. Of the single removal treatments, removal of beetles at 1900 hours was most effective, …

Go to article

Male-Male Mounting And The Unreliability Of Body Size As A Character For Mate Choice In Male Japanese Beetles (Popillia Japonica Newman), Paul V. Switzer, Patrick S. Forsythe, Kipp C. Kruse Jan 2014

Male-Male Mounting And The Unreliability Of Body Size As A Character For Mate Choice In Male Japanese Beetles (Popillia Japonica Newman), Paul V. Switzer, Patrick S. Forsythe, Kipp C. Kruse

Faculty Research & Creative Activity

Background: Same-sex pairing is common in many animal species. In many insects, same-sex pairing is often thought to be a result of poor sexual discrimination (i.e., a mistake), but few detailed studies of the mechanisms underlying the mistaken pairing have been conducted. Previous studies have found that in the field, a small proportion of Japanese beetle (Popillia japonica) mating pairs consist of two males instead of a male and a female. In the current study, we investigated the relationship between body size, the tendency to mount other males, and the duration of these mounts, in laboratory experiments on male Japanese …

Go to article

Trophic Dynamics Of U, Ni, Hg And Other Contaminants Of Potential Concern On The Department Of Energy’S Savannah River Site, Paul G. Edwards, Karen F. Gaines, A. Lawrence Bryan Jr., James M. Novak, Susan A. Blas Jan 2014

Trophic Dynamics Of U, Ni, Hg And Other Contaminants Of Potential Concern On The Department Of Energy’S Savannah River Site, Paul G. Edwards, Karen F. Gaines, A. Lawrence Bryan Jr., James M. Novak, Susan A. Blas

Faculty Research & Creative Activity

The Department of Energy’s Savannah River Site is a former nuclear weapon material production and current research facility located in South Carolina, USA. Wastewater discharges from a fuel and nuclear reactor target manufacturing facility released depleted and natural U, as well as other metals into the Tims Branch- Steed Pond water system. We investigated the current dynamics of this system for the purposes of environmental monitoring and assessment by examining metal concentrations, bioavailability, and trophic transfer of contaminants in seven ponds. Biofilm, detritus, and Anuran and Anisopteran larvae were collected and analyzed for stable isotopes (δ15N, δ13C) and contaminants of …

Go to article

Effectiveness Of Hand Removal For Small-Scale Management Of Japanese Beetles (Coleoptera: Scarabaeidae), Paul Switzer, Ryan Cumming Jan 2014

Effectiveness Of Hand Removal For Small-Scale Management Of Japanese Beetles (Coleoptera: Scarabaeidae), Paul Switzer, Ryan Cumming

Faculty Research & Creative Activity

Hand removal is often recommended as a method for small-scale control of Japanese beetles (Popillia japonica Newman). In this study, we investigated the effectiveness of daily hand removal for controlling damage by Japanese beetles on grape plants. We also investigated whether the timing of the removal (at 0800, 1400, or 1900 hours, or at all 3 periods) inßuenced the effectiveness of the technique. We found that hand removal signiÞcantly lowered the number of beetles on, and consequently the damage to, grape plants relative to nonremoval controls. Of the single removal treatments, removal of beetles at 1900 hours was most effective, …

Go to article

Do Artificial Riffles Enhance Nutrient Retention In Restored Streams?, Adam Thomas Hughes Jan 2014

Do Artificial Riffles Enhance Nutrient Retention In Restored Streams?, Adam Thomas Hughes

Masters Theses

Kickapoo Creek, a tributary of the Embarrass River in Central Illinois, has undergone a stream restoration that included the construction of two artificial riffles. These structures were designed to slow flood waters, increase channel and substrate stability, and increase habitat heterogeneity in a stream impacted by erosion and sediment deposition. They may also provide stable substrates that affect the algal community, leading to shifts in algal community composition, increases in the biomass of primary producers, and subsequent increases in nutrient retention. I explored the effects of the restoration on instream nutrient retention by measuring and comparing phosphate uptake lengths, velocities, …

Go to article

Habitat Associations Of Larval And Juvenile Fishes In A Large Unimpounded River, Sharon V. Rayford Jan 2014

Habitat Associations Of Larval And Juvenile Fishes In A Large Unimpounded River, Sharon V. Rayford

Masters Theses

Larval and juvenile life stages of fish are numerically the dominant component of fish populations. These early life stages experience high levels of mortality due, in part, to anthropogenic disturbances, but little is known about which habitats they utilize in large-rivers, as they are often difficult to sample and identify. My study examined the larval and juvenile fish assemblages in different habitats within the lower 322 km of the Wabash River. Fish were sampled with a conical-cylindrical ichthyoplankton net (larvae) and a DC-electrified mini-Missouri trawl (juveniles) between May and October 2013. Larval fish were collected every two weeks from mid-May …

Go to article

Demographics And Seasonal Diet Composition Of Shovelnose Sturgeon (Scaphirhynchus Platorynchus Rafinesque) In Wabash River, Vaskar Nepal Jan 2014

Demographics And Seasonal Diet Composition Of Shovelnose Sturgeon (Scaphirhynchus Platorynchus Rafinesque) In Wabash River, Vaskar Nepal

Masters Theses

Shovelnose sturgeon Schaphirhynchus platorynchus Rafinesque, one of the only sturgeon species that support a sustainable commercial harvest, are fished in a substantial caviar fishery in the lower Wabash River. However, ecological information on the population is sparse. In this thesis, I present information on the status and seasonal diet characteristics of the population. A 14-year long shovelnose sturgeon monitoring survey conducted by Illinois Department of Natural Resources shows that the population is in relatively good condition, faces low mortality rates, and has a high potential for recruitment. Study of the seasonal composition of diet suggests that shovelnose sturgeon generally get …

Go to article

The Effects Of Aerobic And Muscular Endurance Based Resistance Exercises On The Cardiorespiratory System In College Age Females, Victoria Streif Jan 2014

The Effects Of Aerobic And Muscular Endurance Based Resistance Exercises On The Cardiorespiratory System In College Age Females, Victoria Streif

Masters Theses

The purpose of this study was to evaluate the cardiorespiratory response in college age females, between the ages of 19 and 24, before and after participating in a six week moderate-vigorous aerobic exercise program compared to a six week moderate-vigorous aerobic exercise program combined with a muscular endurance based resistance training program. Specifically, which mode of exercise has the most significant effect on cardiorespiratory fitness? Methods: Twelve sedentary college age females between the ages of 19 to 24 years, with a mean age of 20.9 years, participated in the study. Eligible participants were randomly assigned to one of three groups: …

Go to article

Aspects Of The Trophic Ecology Of An Invertivorous Snake Community, Meagan Amanda Thomas Jan 2014

Aspects Of The Trophic Ecology Of An Invertivorous Snake Community, Meagan Amanda Thomas

Masters Theses

Understanding the significance of trophic links has been of interest to ecologists for decades, likely because food web studies have the potential to reveal a considerable amount of information in the fields of ecosystem and community ecology. Despite the intrinsic benefits that come from elucidating food web structures, doing so is often problematic because of the complex and dynamic nature of ecological communities. The dietary ecology of small-bodied invertivorous snakes remains relatively understudied compared to other snake species. Many of these species are abundant throughout their range, making them ideal organisms for studying community-level questions. I employed a combination of …

Go to article

Wastewater Influent And Effluent Concentrations Of 17 Beta-Estradiol: A Study Of The Influence Of A University Community And The Risk To Environmental Health, Kyle Heffron Jan 2014

Wastewater Influent And Effluent Concentrations Of 17 Beta-Estradiol: A Study Of The Influence Of A University Community And The Risk To Environmental Health, Kyle Heffron

Masters Theses

The concentration of 17β-estradiol (E2) was measured through stages of wastewater treatment at a central Illinois wastewater treatment plant (WWTP). E2 concentration was quantified using a competitive enzyme linked immunosorbent assay (ELISA). The concentration of E2 was compared with demographic effects of a university, physical parameters of the wastewater (dissolved oxygen, pH, and temperature) and daily influent and effluent flow rates. Effluent concentrations ranged from 0 to 25.3 ng L-1 with an average discharge of 3.6 ng L-1. E2 concentration was shown to increase at the start of each university semester, however, this trend was not observed …

Go to article

Examining The Nutritional Requirements Of Acidophilic Archaea "Ferroplasma Acidarmanus" Strain Fer1, Yudong Qu Jan 2014

Examining The Nutritional Requirements Of Acidophilic Archaea "Ferroplasma Acidarmanus" Strain Fer1, Yudong Qu

Masters Theses

Ferroplasma acidarmanus strain fer1 (fer1) is an archaeal microaerophilic acidophile (pH 0 to pH 3) with a chemolithotrophic metabolism. As an efficient consumer of sulfate, fer1 plays an important role in the biogeochemical process of pyrite dissolution at acidic site. Confirming the genomic prediction of metabolic gene functions in this organism requires functional assays, which cannot be carried out until a defined medium is obtained. The objective of the current study was to replace the 0.1% (w/v) yeast extract component in the growth medium, mfer, with defined components for carbon, nitrogen, and vitamin needs. In order to replace the 0.1% …

Go to article

The Effects Of 1, 3, & 7 Trisubstituted And 8-Cyclopentyl Alkylxanthines On Respiration In Newborn Rats, Nathan Creel Davidson Jan 2014

The Effects Of 1, 3, & 7 Trisubstituted And 8-Cyclopentyl Alkylxanthines On Respiration In Newborn Rats, Nathan Creel Davidson

Masters Theses

Methylxanthines have been shown to be promising respiratory stimulants in newborns suffering from recurrent apnea. The respiratory stimulant effects of methylxanthines are most likely due to their antagonism of adenosine receptors. These drugs have been shown to stimulate respiration in newborn rats in doses in excess of 10 mg/kg body weight. Structure-activity studies have shown that methylxanthines not only differ in the antagonism of A1 and A2 adenosine receptors but also, as a consequence of their different chemical structures, differ in potency as respiratory stimulants. To assess this, two potent A1 adenosine receptor antagonists, 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) and …

Go to article

Effects Of Dams On Fish And Macroinvertebrate Communities In The Vermilion River, Il, Ryan Patrick Hastings Jan 2014

Effects Of Dams On Fish And Macroinvertebrate Communities In The Vermilion River, Il, Ryan Patrick Hastings

Masters Theses

Dams are a main source of anthropogenic disturbances on river systems and can affect rivers in a variety of ways. Dams have the ability to change rivers from lotic to lentic habitats, affect sediment transportation, connectivity, water quality, linkages with wetlands and the quality of in-stream and riparian habitats. The Danville Dam was constructed in 1914 on the Vermilion River in Danville, Illinois and is becoming a safety hazard for human recreation on the Vermilion River, resulting in three deaths in the last 10 years. The Illinois Department of Natural Resources in conjunction with the city of Danville has proposed …

Go to article

Comparison Of Oak And Sugar Maple Distribution And Regeneration In Central Illinois Upland Oak Forests, Peter J. Frey Jan 2014

Comparison Of Oak And Sugar Maple Distribution And Regeneration In Central Illinois Upland Oak Forests, Peter J. Frey

Masters Theses

Although white oak (Quercus alba) dominated much of the mid-west and eastern US hardwood forests prior to European settlement, changes in disturbance frequencies and habitat fragmentation, coupled with other biotic pressures, are allowing sugar maple (Acer saccharum Marshall) to displace oak in the upland forest understory. Since our understanding of how disturbance mechanisms influence oak regeneration is not fully clear, there is lack of consensus on how to employ management practices. We collected seedling microhabitat data from 5 upland oak forest sites in central Illinois, each differing in age class and/or silvicultural treatment to determine: 1) Whether …

Go to article

The Effects Of Theophylline And 8-Cyclopentyltheophylline On The Respiratory Response To Carbon Dioxide In Neonatal Rats, Christine E. Albers Jan 2014

The Effects Of Theophylline And 8-Cyclopentyltheophylline On The Respiratory Response To Carbon Dioxide In Neonatal Rats, Christine E. Albers

Masters Theses

Premature infants are often plagued with respiration problems ranging from periodic breathing to apnea. These respiratory complications can be a result of underdeveloped respiratory organs, immaturity of the brainstem respiratory control center, genetic irregularities, or a combination of all three. This study sought to increase respiration with the administration of methylxanthines, which are respiratory stimulants, coupled with carbon dioxide (CO2), also a respiratory stimulant. Neonatal rats aged 4 to 7 days old were used to mimic premature infants' response to the interaction of methylxanthines and CO2. Before beginning the respiratory studies, sections of a 4- and …

Go to article

Functions Of Mitoneet: What The Protein-Protein Interactions Reveal, Fran Hirschfelder Jan 2014

Functions Of Mitoneet: What The Protein-Protein Interactions Reveal, Fran Hirschfelder

Masters Theses

MitoNEET is a [2Fe-2S] cluster protein found on the outer mitochondrial membrane. First discovered as a binding site for pioglitazone, an anti-diabetic drug, it was the first protein described containing a CDGSH iron-sulfur domain (CISD). However, the function(s) of mitoNEET in both normal cellular physiology and the physiology in type-2 diabetes remains unclear. The proposed functions of mitoNEET, include cluster transfer, electron transport and oxidative respiration. This thesis describes the use of proteomic pull-down techniques to identify possible protein binding partners of mitoNEET from both mouse and human liver cell lysates. In order to accomplish this, the expression and purification …

Go to article

A Comparison Of The Effects Of Static Stretching With And Without Whole Body Vibration On Hip Flexion Range Of Motion In College Age Males, Jessica Lynn Wilson Jan 2014

A Comparison Of The Effects Of Static Stretching With And Without Whole Body Vibration On Hip Flexion Range Of Motion In College Age Males, Jessica Lynn Wilson

Masters Theses

The purpose of this study was to compare the effects of a single bout of static stretching with and without whole body vibration on hip flexion range of motion in college age males. A second purpose was to determine whether any acute effects would persist after one hour of rest. It was hypothesized that there would be a significant increase from baseline to posttest hip range of motion with both static stretching only and static stretching with whole body vibration. Further, the improvement would be significantly greater with the addition of whole body vibration. It was also hypothesized that any …

Go to article

Assessment Of Range, Habitat Use, And Diel Movement Of Flathead Catfish (Pylodictis Olivaris) In The Wabash River Using Ultrasonic Telemetry, Sarah Mary Huck Jan 2014

Assessment Of Range, Habitat Use, And Diel Movement Of Flathead Catfish (Pylodictis Olivaris) In The Wabash River Using Ultrasonic Telemetry, Sarah Mary Huck

Masters Theses

Flathead Catfish (Pylodictis olivaris) are one of the most targeted species for recreational and commercial fishing within the Mississippi River Basin. With heavy harvest pressure placed on many populations, proper management is crucial; however, the species range, habitat requirements, and diel movement patterns have rarely been investigated in free flowing rivers. Therefore, I used ultrasonic telemetry with active tracking to monitor movement and habitat use of 44 Flathead Catfish from August 2012 to December 2013 in the lower 280 km of the Wabash River. This system is ideal to evaluate broad and fine scale movement of Flathead Catfish …

Go to article

Morphological And Ecological Investigations Of Species Of Bulrush (Scirpus) In Illinois, Julian G. Moore Jan 2014

Morphological And Ecological Investigations Of Species Of Bulrush (Scirpus) In Illinois, Julian G. Moore

Masters Theses

Two closely related species of bulrush, Scirpus atrovirens (Green bulrush) and Scirpus georgianus (Georgia bulrush), are widely distributed in Illinois. They are difficult to separate in the field, but readily distinguishable under magnification in the lab. These two species have been found in moist meadows, shallow marshes, edges of wet forests, and ditches. The objective of this study was to evaluate the morphological and ecological distinctness of the species with the aim toward discovering and describing other features to distinguish them reliably. Eighty plants of Scirpus atrovirens and 80 of Scirpus georgianus were examined. Fifty S. atrovirens and 50 S. …

Go to article

Comparison Of Muscular Strength And Hypertrophy In High Repetition Training Versus Low Repetition Training For The Bench Press Exercise, Tyler Mink Jan 2014

Comparison Of Muscular Strength And Hypertrophy In High Repetition Training Versus Low Repetition Training For The Bench Press Exercise, Tyler Mink

Masters Theses

No abstract provided.

Go to article

The Role Of Landmarks In Territory Maintenance By The Black Saddlebags Dragonfly, Tramea Lacerata, Jeffrey Lojewski Jan 2014

The Role Of Landmarks In Territory Maintenance By The Black Saddlebags Dragonfly, Tramea Lacerata, Jeffrey Lojewski

Masters Theses

Territoriality can reduce competition for resources, but territorial defense can be costly. Therefore any behavior that reduces territorial costs may increase the net benefit of territoriality. Some species will align their territory boundaries with conspicuous landmarks that may serve to reduce defense costs. Dragonflies, including black saddlebags (Tramea lacerata), defend territories at breeding sites, keeping rival males away to allow themselves access to females. We used three treatments to investigate whether T. lacerata used landmarks: constraining landmarks (an object that provided a physical barrier to flight), non-constraining landmarks (an object of the same dimensions and construction that did …

Go to article

Effects Of Instream Habitat Restoration On Macroinvertebrate And Fish Communities In A Small Midwestern Stream, Manisha Pant Jan 2014

Effects Of Instream Habitat Restoration On Macroinvertebrate And Fish Communities In A Small Midwestern Stream, Manisha Pant

Masters Theses

Although millions of dollars are spent annually on stream habitat restoration, the biological responses to these restorations are rarely monitored. I assessed the impact of habitat enhancement on fish and macroinvertebrate abundance and diversity in a small Midwestern stream. Fish and macroinvertebrate communities were sampled annually in two restored and two unrestored sites beginning two years before and continuing four years after restoration. Habitat heterogeneity increased in the restored sites following the restoration, but there were no significant changes in most macroinvertebrate and fish community indices. Macroinvertebrate composition was significantly different between spring and fall, but in general, assemblages within …

Go to article

Identification Of Disulfide Bond Formation Between Mitoneet And Glutamate Dehydrogenase 1, Morgan E. Roberts, Jacquelyn P. Crail, Megan M. Laffoon, William G. Fernandez, Michael A. Menze, Mary E. Konkle Dec 2013

Identification Of Disulfide Bond Formation Between Mitoneet And Glutamate Dehydrogenase 1, Morgan E. Roberts, Jacquelyn P. Crail, Megan M. Laffoon, William G. Fernandez, Michael A. Menze, Mary E. Konkle

Faculty Research & Creative Activity

MitoNEET is a protein that was identified as a drug target for diabetes, but its cellular function as well as its role in diabetes remains elusive. Protein pull-down experiments identified glutamate dehydrogenase 1 (GDH1) as a potential binding partner. GDH1 is a key metabolic enzyme with emerging roles in insulin regulation. MitoNEET forms a covalent complex with GDH1 through disulfide bond formation and acts as an activator. Proteomic analysis identified the specific cysteine residues that participate in the disulfide bond. This is the first report that effectively links mitoNEET to activation of the insulin regulator GDH1.

Go to article

Effects Of Rest Interval Length On Smith Machine Bench Press Performance And Perceived Exertion In Trained Men, Ramires A. Tibana, Denis C. Vieira, Vitor Tajra, Martim Bottaro, Belmiro F. De Salles, Jeffrey Willardson, Jonato Prestes Dec 2013

Effects Of Rest Interval Length On Smith Machine Bench Press Performance And Perceived Exertion In Trained Men, Ramires A. Tibana, Denis C. Vieira, Vitor Tajra, Martim Bottaro, Belmiro F. De Salles, Jeffrey Willardson, Jonato Prestes

Faculty Research and Creative Activity

This study compared two different rest intervals (RI) between sets of resistance exercise. Ten resistance-trained men (M age = 24.3, SD = 3.5 yr.; M weigh t= 80.0 kg, SD = 15.3; M height = 1.75 m, SD = 0.04) performed five sets of Smith machine bench presses at 60% of one repetition maximum, either with 1.5 min. or 3 min. RI between sets. Their repetition performance, total training volume, velocity, fatigue, rating of perceived exertion, and muscular power were measured. All of these measures indicated that performance was significantly better and fatigue was significantly lower in the 3 min. …

Go to article

Differential Habitat Use By Common Watersnakes (Nerodia Sipedon), Lorin A. Neuman-Lee, Andrew M. Durso, Nicholas M. Kiriazis, Melanie J. Olds, Stephen J. Mullin Dec 2013

Differential Habitat Use By Common Watersnakes (Nerodia Sipedon), Lorin A. Neuman-Lee, Andrew M. Durso, Nicholas M. Kiriazis, Melanie J. Olds, Stephen J. Mullin

Faculty Research & Creative Activity

Understanding intraspecific variation in habitat use is important for the management of any species. In many studies of reptiles, habitat use by juveniles is poorly understood when compared to their adult conspecifics because of capture biases and logistical constraints. We compared habitat use between sexes and age classes of Common Watersnakes (Nerodia sipedon) at a reservoir in central Illinois. Juvenile N. sipedon occurred more frequently in habitat with high canopy cover, whereas adult N. sipedon, especially reproductive females, were found exclusively in habitat with no canopy cover. Adult males used both locations equally. We emphasize the need to investigate ontogenetic …

Go to article

Identification Of Disufide Bond Formation Between Mitoneet And Glutamate Dehydrogenase 1, Morgan E. Roberts, Jacquelyn P. Crail, Megan M. Laffoon, William G. Fernandez, Michael A. Menze, Mary E. Konkle Dec 2013

Identification Of Disufide Bond Formation Between Mitoneet And Glutamate Dehydrogenase 1, Morgan E. Roberts, Jacquelyn P. Crail, Megan M. Laffoon, William G. Fernandez, Michael A. Menze, Mary E. Konkle

Faculty Research & Creative Activity

MitoNEET is a protein that was identified as a drug target for diabetes, but its cellular function as well as its role in diabetes remains elusive. Protein pull-down experiments identified glutamate dehydrogenase 1 (GDH1) as a potential binding partner. GDH1 is a key metabolic enzyme with emerging roles in insulin regulation. MitoNEET forms a covalent complex with GDH1 through disulfide bond formation and acts as an activator. Proteomic analysis identified the specific cysteine residues that participate in the disulfide bond. This is the first report that effectively links mitoNEET to activation of the insulin regulator GDH1.

Go to article

Leaf Anatomy And Systematics Of Polygaleae (Polygalaceae), Heather Wilber Dec 2013

Leaf Anatomy And Systematics Of Polygaleae (Polygalaceae), Heather Wilber

Undergraduate Honors Theses

Polygalaceae are comprised of approximately 1000 species found worldwide in a variety of habitats. Over half of the species in the family were traditionally grouped into the polyphyletic genus Polygala and need resolution. Leaves from 21 species in 13 genera within tribe Polygaleae were field collected or taken from specimens at the Missouri Botanical Garden Herbarium (MO) to find phylogenetically useful characteristics of the foliar anatomy. The leaves were rehydrated using concentrated ammonium hydroxide, embedded in paraffin, and sectioned at 10 μm thickness using a rotary microtome. To observe epidermal features, rehydrated leaves were also cleared using 3% potassium hydroxide. …

Go to article

Optimization Of Protocols For Wolbachia Detection Using Pcr Methods, Jennifer Kim Dec 2013

Optimization Of Protocols For Wolbachia Detection Using Pcr Methods, Jennifer Kim

Undergraduate Honors Theses

Wolbachia is a genus of intracellular bacterium that is known to infect a wide range of arthropods. Species within this clade have the ability to modify the reproductive success of their hosts to promote their own distribution throughout host populations. Wolbachia cannot be cultured outside of the host and characterizing infection of gonadal cells might be time consuming. Thus, polymerase chain reaction (PCR) assays are a common method of Wolbachia DNA amplification from arthropods. I assessed the utility of 3 DNA sequence markers (16S, ITS, and 28S) paired with the Wolbachia surface protein (wsp) gene in various PCR …

Go to article

Comparison Of Theophylline And 8-Cyclopentyltheophylline On The Heart Rates Of Neonatal Rats, Stephanie Arnold Dec 2013

Comparison Of Theophylline And 8-Cyclopentyltheophylline On The Heart Rates Of Neonatal Rats, Stephanie Arnold

Undergraduate Honors Theses

Neonatal apnea is a serious condition that affects the health of infants, especially those born prematurely. To stimulate breathing, methylxanthine drugs, such as theophylline and caffeine, are administered to reverse this condition in humans and avoid the use of breathing-assistance equipment. These drugs cause stimulation of the central nervous system and the heart, however, both detrimental side effects in preterm infants. Antagonism of adenosine receptors is the proposed mechanism of methylxanthine action. Synthetic chemists have produced xanthine analogs with increased affinity for adenosine receptors that could lead to respiratory stimulation while reducing cardiostimulant effects. For this study, theophylline and 8-cyclopentyltheophylline …

Go to article

First Prev Next Last