Life Sciences Commons^™

The Epigenetic Impact Of Dietary Choline On Growth Outcomes Of Children Exposed To Gestational Diabetes Mellitus, Ismail Kadam

Dissertations, Theses, and Capstone Projects

Maternal insulin resistance and hyperglycemia lead to gestational diabetes mellitus (GDM) which can cause fetal hyperinsulinemia, ultimately leading to excessive fetal growth associated with macrosomia and increased adiposity at birth. Increased placental macronutrient transport and epigenetic changes in growth and metabolic genes affected by GDM contribute to fetal overgrowth and can result in complications such as overweight/obesity, insulin resistance/type 2 diabetes, cardiovascular disease risk factors, as well as cognitive impairment later in life. Choline, an essential nutrient, participates in lipid and energy metabolism by providing methyl groups for DNA methylation and thus may overcome the hypomethylation of growth and metabolic …

Competitive And Noncompetitive Credit Assignment To Reward Cues In The Lateral Orbitofrontal Cortex, Mihwa Kang

Dissertations, Theses, and Capstone Projects

This dissertation investigates the mechanisms underlying variations in credit assignment to reward cues, with a focus on the lateral orbitofrontal cortex (lOFC). The lOFC has long been implicated in signaling reward expectancies, with controversy as to whether it signals the economic value of future outcomes, their sensory features to guide behavior or both. However, whether the lOFC signals the relative (competition-dependent) or absolute (contiguity-dependent) credit of cues is unknown. To address this, I employed a combination of novel behavioral assays, in-vivo electrophysiological recordings and optogenetic stimulation in rats. In Chapter 2, I tested the suitability of a self-initiating variant of …

Effects Of Brca1-Mediated Ubiquitination Of Hur On Rna Metabolism In Breast Cancer, Devorah Mincha Natelson

Dissertations, Theses, and Capstone Projects

BRCA1, a gene encoding for the tumor suppressor protein BRCA1, is involved in DNA repair as well as many other important cellular processes. BRCA1, together with BARD1 (BRCA1 Associated RING Domain 1) also functions as an E3 ubiquitin (Ub) ligase. BRCA1 mutation in the RING domain, which is responsible for E3 ligase activity, is associated with increased risk of developing breast cancer. Despite extensive research on the activities of BRCA1, the E3 ligase activity and implications of this activity in cancer are not well understood. This dissertation examines the ubiquitination of the RNA binding protein Human antigen R (HuR) by …

Resting-State Eeg Microstate Features For Major Depressive Disorder Classification, George M. V. Quinn

Dissertations, Theses, and Capstone Projects

Neuroimaging studies have revealed consistent abnormalities in functional connectivity within specific neural networks that may serve as biomarkers for major depressive disorder (MDD). It is important to find inexpensive, non-invasive techniques that target these biomarkers to make diagnosis easier and more objective. EEG microstates are quasi-stable potential topographies that are thought to reflect the quasi-stable network activity of the underlying neural generators. MDD has been shown to alter features of the four canonical EEG microstates (A, B, C, D) with some conflicting results. The most consistent network abnormalities in MDD are found in the anterior default mode network, and this …

Voltage Set Points For Sodium Channel Inactivation: Intrinsic And Modulatory Mechanisms, Akshay Sharma

Dissertations, Theses, and Capstone Projects

During an action potential, voltage-gated sodium channels (VGSC) control sodium ion flow into the cytoplasm of cells by using membrane voltage to alter channel conformation. These membrane proteins exhibit three main functional conformations: closed, open, and fast-inactivated, with transitions between these states dependent on membrane voltage and kinetics. The channels open within 1 millisecond and swiftly transition from open to fast-inactivated within 1-2 milliseconds following membrane depolarization. Subsequent membrane repolarization resets the channels to the closed state. Notably, even at mildly depolarized potentials that preclude channel opening, VGSCs can undergo transitions between the closed and inactivated states, known as closed-state …

Newly Synthesized Pyroglutamate Amide And Its Neuroprotective Effect Against Neurotoxicity Induced Cell Death In Alzheimer's Disease, Nourelhouda Joutey Elamrani

Dissertations, Theses, and Capstone Projects

The neurodegenerative disorder is an umbrella term for a range of conditions that affects the neurons in the human brain causing neuronal cell death. The common pathway is the excitotoxicity, a phenomenon that describes the toxic actions of excitatory neurotransmitters, primarily glutamate, where the exacerbated or prolonged activation of glutamate receptors starts a cascade of neurotoxicity leading to the cell death. One of the most common neurodegenerative disorders is Alzheimer’s disease (AD), which represents 60 – 70% of cases and it ranked fourth cause of mortality in the USA. In Alzheimer’s disease, glutamate released from astrocytes and activates extrasynaptic N-methyl-D-aspartate …

Exploiting The Heavy Chain Glycans For The Improvement Of Radioimmunoconjugates, Cindy Rodriguez

Dissertations, Theses, and Capstone Projects

Radiolabeled antibodies have become indispensable tools in nuclear medicine. However, the natural roles of antibodies within the immune system mean that they have several intrinsic limitations as a platform for radiopharmaceuticals. A handful of recent preclinical studies suggest that binding by FcR and particularly FcyRI can affect the pharmacokinetic profiles of 89Zr-labeled radioimmunoconjugates. Fc receptors (FcR) are responsible for many of the interactions between immunoglobulins (IgG) and immune cells. In biomedicine, this interplay is critical to the activity of several types of immunotherapeutics; however, relatively little is known about how FcRs affect the in vivo performance of radiolabeled antibodies. Antibodies …

Guardians Of The Bacterial Genome: Mechanistic Insights Of The Ner Atpase Uvra With Damaged Dnas; Molecular Interactions Between The V.Cholerae Initiator Rctb With Cognate Oricii Dna, Devon Semoy

Dissertations, Theses, and Capstone Projects

Nucleotide excision repair (NER) is one of the fundamental DNA repair pathways in all organisms. A unique facet of NER is the large set of DNA lesions the pathway is capable of repairing (Truglio, Croteau, et al. 2006). The NER proteins may thus be responding to global features of native DNA such as changes in thermodynamic stability, geometry and kinetic properties (Maillard et al. 2007). In this study we set out to investigate the first and primary responder to NER in bacteria, UvrA (Kraithong et al. 2021). UvrA is a homo-dimeric ABC ATPase; proteins that are known to transduce the …

Understanding Primate Immunity To African Trypanosomes Through The Characterization Of Trypanosome Lytic Factor Formation And Function, Sara Fresard

Dissertations, Theses, and Capstone Projects

African trypanosomes cause an annual loss of over 1 billion USD due to their economic and agricultural burden in Sub-Saharan Africa. These parasites are unicellular, extracellular parasites, that infect humans, cattle, and other livestock. Humans and some non-human primates are protected against most species of trypanosomes due to an innate immunity factor, Trypanosome Lytic Factor (TLF). This modified High Density Lipoprotein (HDL) contains two unique proteins, Haptoglobin Related Protein (HPR), a ligand to a trypanosome surface receptor, and Apolipoprotein-L1 (APOL1), the lytic component. Our overall goal is to use our understanding of primate immunity to create genetically modified cattle that …

Meconium: A Potential Means To Predicting Later-Life Cognition, Leah Duran

Dissertations, Theses, and Capstone Projects

Maternal perinatal stress is a commonly experienced, yet chronically undiagnosed condition (CDC, 2010). Literature has suggested that perinatal stress exposure has long lasting implications of the developing child both physically and mentally (Behrman et al., 2007; Hobel et al., 2008; Bowman et al., 2004; Polanska et al., 2017; Van de Bergh et. al, 2020). The use of meconium in the clinical setting has become one that serves a purpose of detection in medical conditions like in utero drug exposure. Using meconium as a predictor for brain behavior, however, is still an emerging concept in the literature. It is quickly gaining …

The Seat Of Singular Gene Choice: Dissecting The Role Of Odorant Receptor Enhancer Elements That Lead To Mammalian Olfactory System Expression, Alessandro M. Rosa

Dissertations, Theses, and Capstone Projects

Odorant receptors (ORs) were first identified in 1991 and the initial data strongly suggested that ORs were part of a large superfamily of G-protein-coupled receptors. Within a few years of the discovery of the OR superfamily, it became clear that each OR allele was exclusively expressed in olfactory sensory neurons (OSN), and that this singular expression leads to cellular identity. Many studies over the past thirty years have sought to unlock the mysteries of the mechanism for monoallelic and monogenic OR gene activation and expression in OSNs, however no definitive model for singular gene expression has emerged.

Using a series …

Autophagy And Meiotic Fidelity In C. Elegans, Kaitlin E. Kosinski

Dissertations, Theses, and Capstone Projects

Autophagy is an evolutionarily conserved multi-step recycling process in which cellular material is enclosed in the double membrane-bound autophagosome, which fuses with the lysosome to degrade its contents. Autophagy is essential for development and cellular adaptation to environmental or intracellular stress and is an important regulator of germline stem cell homeostasis in the model nematode C. elegans. We sought to determine if autophagy is important for genome stability during meiosis and found that the core adult C. elegans autophagy genes bec-1, atg-7, unc-51, and atg-18 were all required for proper meiotic development of oocytes. Loss of these …

Investigation Of The Prevalence Of Fentanyl Analogs In A Drug Testing Population Using Liquid Chromatography Tandem Mass Spectrometry (Lc-Ms-Ms), Ivan Cordova

Student Theses

This study serves to determine the prevalence of novel fentanyl analogs that are present within a drug testing population in New York State. Samples were analyzed using liquid chromatography tandem mass spectrometry (LC-MS/MS) to qualitatively determine the presence of various possible fentanyl analogs. The sample preparation of this assay consisted of a simple dilute and shoot. This method of sample preparation can be quicker, eliminating sample preparation times as well as being less financially strenuous to clinical laboratories as it eliminates the need for various solvents and solid/liquid phase cartridges. The assay was aimed to see if analytes can be …

Analyzing Hiv/Aids Trends And Disparities In The Bronx: A Comparative Study With Nyc Boroughs, Caleb Adjei

CUNY Mexican Studies Institute

This study analyzes changes in reported HIV/AIDS cases over time in the Bronx, identifying the racial/ethnic groups and neighborhoods most affected by the disease. The Bronx consistently exhibits the highest rate of new HIV diagnoses among New York City’s boroughs, with Latino/Hispanic and Black populations being disproportionately impacted. Key neighborhoods like High Bridge-Morrisania, Crotona-Tremont, and Fordham-Bronx Park have the highest case concentrations. Despite advancements in healthcare and treatments like antiretroviral therapy, disparities persist. The findings highlight the need for targeted interventions to support the most affected communities and address the underlying social determinants contributing to the disparities in HIV/AIDS diagnoses.

The Effects Of People, Time Of Day, And Season On Affiliative And Stereotypic Behaviors In Captive Rhinoptera Bonasus And Other Rays Across Two Human-Interactive Enclosures, Wren C. A. Newman

Theses and Dissertations

Cownose rays (Rhinoptera bonasus) are among the most common species found in aquaria, yet the impact of touch enclosures on their welfare remains largely unknown. While research on animal-visitor interactions has produced mixed results for other species, the lack of studies focusing on fish welfare poses a risk of overlooking potential welfare concerns. Therefore, we aimed to establish a baseline for cownose rays regarding stereotypic behaviors, sociality, and activity in captivity, and to examine the influence of human interaction on these behaviors. We collected over 700 hours of footage from two enclosures at Adventure Aquarium in Camden, NJ …

Sex Differences In The Diets Of Forest-Dwelling Baboons (Papio Anubis), Kara Hartunian

Theses and Dissertations

The ability to eat and switch between many food sources is an adaptative feeding strategy utilized for meeting nutritional needs across different environments, seasons, and physiological conditions. Baboons (genus Papio) excel at both dietary flexibility and foraging selectively, and consequently have one of the most widespread geographic distributions in the Order. To further expand to our understanding of baboon feeding ecology, I analyzed foraging activity and the nutritional characteristics of the diets of the relatively understudied rainforest baboons (Papio anubis) living in Kibale National Park, Uganda. I also investigated if there are differences in food type choice …

Assessment Of Tripeptides Self-Assembly & Er Stress On Cell Viability And Exosome Secretion In Mda-Mb231, Azmat Parveen

Theses and Dissertations

This study delves into the effects of tripeptides KYpF and WYpK(NBD) on MDA-MB-231 cells, uncovering KYpF's ability to reduce cell proliferation and exosome secretion in peptide treated cells. Conversely, WYpK(NBD) demonstrated significant toxicity at 10 μM. These insights pave the way for optimizing peptide-based cellular treatments for exosome secretion purposes.

Maternal And Other Social Influences On Signature Whistle Structure Of A Captive Group Of Bottlenose Dolphins (Tursiops Truncatus), Riley Synan

Theses and Dissertations

Bottlenose dolphins (Tursiops truncatus) are social animals that use acoustics as a major method of communicating across a marine environment. They also live in fission-fusion groups and thus interact with many conspecifics over a lifetime. Individual dolphins have a signature whistle that functions as an identity call and serves to maintain group cohesion in murky waters or during reconciliation. Dolphins exhibit vocal learning and the signature whistle of a calf may be modeled after the whistle of their mother or another individual in the pod as they develop. Mother-calf pairs or other related animals sometimes have similar whistle types. Some …

Assessing Comprehension And Competence In Bottlenose Dolphins (Tursiops Truncatus) On The Concept: Do Something Different, Charlotte M. Mulligan

Theses and Dissertations

Many studies have been conducted using a training paradigm based on the research of Pryor et al. (1969) wherein the participants are trained on the abstract concept: do something different. This paradigm challenges working memory and requires the participants to respond with a behavior, either previously trained or novel, which has not yet been offered within the session. The work of Braslau-Schneck (1994) expanded this concept and demonstrated the ability of dolphins to successfully complete this task in synchronized pairs, though the method of accomplishing this task remained unclear. The present research was conducted as part of a broader …

Mapping Urban Tree Canopy Using Publicly Available Satellite Data, Rosemary Mcguinness

Theses and Dissertations

This project addresses the need for accessible, cost-effective tools for quantifying spatial and temporal changes in tree canopy cover in urban areas. Urban tree canopy provides a wide range of ecosystem services, including lowering air temperatures, reducing pollution, and mitigating stormwater runoff. Cities around the world have placed the expansion of their urban forests at the center of their sustainability goals. Consistent and timely data on urban tree canopy is essential for urban greening initiatives to succeed. Existing methods of accessing information about urban tree canopy are highly technical, costly, and labor-intensive, while the freely available source of tree canopy …

Effortless Mindfulness Toolkit: Easy Meditation Scripts To Begin Each Class., Brett Whysel

Open Educational Resources

The Effortless Mindfulness in the Classroom Toolkit is an open educational resource designed to create happier classrooms, boost student engagement, and foster a sense of community through brief, effective mindfulness exercises. This comprehensive toolkit provides educators with everything they need to seamlessly integrate mindfulness practices into their teaching routines, regardless of prior experience. The toolkit includes introductory slides, 15 ready-to-use meditation scripts, and accompanying audio recordings, covering a variety of mindfulness techniques such as focused breathing, body scans, and loving-kindness meditations. Each meditation is paired with a reflective prompt to deepen student engagement and reinforce the benefits of mindfulness. Additionally, …

Querymate: A Custom Llm Powered By Llamacpp, Pegah Khosravi

Open Educational Resources

No abstract provided.

Interleukin 24 Induces Apoptosis Through Glycogen Synthase Kinase-3 Beta Inactivation In Prostate Cancer Cells, Sual J. Lopez, Moira Sauane

Theses and Dissertations

Interleukin 24 (IL-24) is a tumor-suppressing protein currently in clinical trials. IL-24 induces cancer-specific apoptosis by activating endoplasmic reticulum (ER) stress and mitochondria dysfunction. We have previously demonstrated that IL-24 leads to apoptosis in cancer cells by protein kinase A (PKA) activation in human breast cancer cells. To further understand the mechanism by which IL-24 induces apoptosis, I analyzed the role of glycogen synthase kinase-3 (GSK3), a highly conserved and normally active serine/threonine kinase in cancer cells and downstream target of PKA. The work reported here provides direct evidence that GSK3 was inhibited following IL-24 treatment in human prostate cancer …

Editorial For "Parallel Cnn-Deep Learning Clinical-Imaging Signature For Assessing Pathologic Grade And Prognosis Of Soft Tissue Sarcoma Patients", Pegah Khosravi

Publications and Research

No abstract provided.

Proceedings Of The Cuny Games Conference 10.0, Robert O. Duncan, Grace Axler-Diperte, Joe Bisz, Christina Boyle, Devorah Kletenik, Carolyn Stallard

Publications and Research

The ten-year anniversary (!) of the CUNY Games Conference combines workshops, idea exchanges, interactive participant presentations, playtesting, and playing tabletop games into a two-day hybrid event to promote and discuss game-based learning. The conference focuses on creative pedagogy, such as playful learning activities or games, that teachers can use in the classroom every day. Day 1 featured interactive presentations by attendees, informal idea exchange sessions, and workshops by the conference organizers. Day 2 featured select presentations and workshops, poster sessions, playtesting and game modding, and casual play of tabletop games.

Effect Of Plumbagin On Chemo-Resistant Metastatic Retinoblastoma, John J. Soto

Theses and Dissertations

Retinoblastoma, which is an ocular malignancy, usually results in poor prognoses in pediatric patients worldwide. Retinoblastoma in some events, can develop metastatic phenotypes which can lead to secondary tumor formation, furthering deleterious patient outcomes. It is of paramount importance to identify and research potent novel compounds that can be used to increase the likelihood of remission. Plumbagin (PLB) is a plant-derived, neuroprotective agent, which exhibits significant anticancer activities during in vitro study. PLB has been shown to have a high therapeutic efficacy against chemoresistant sublines as well as their normal counterparts. We attempted to show that the chemoresistant ABCC1 could …

A One Health Approach To Enhancing Sars-Cov-2 Surveillance, Lauren C. Cybulska

Dissertations and Theses

Background

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the virus that causes coronavirus disease-2019 (COVID-19) in humans, can also infect domestic dogs (Canis lupus familiaris) and cats (Felis catus). Susceptibility and viral transmissibility in these species are concerns for public health due to the spillover risk to humans or other animals. This dissertation uses a One Health framework to explore key issues on this topic. We aim to identify gaps in understanding of SARS-CoV-2 in companion animals and investigate novel surveillance methods through human, animal, and environmental health perspectives.

Methods

Paper 1 provides an in-depth exploration of …

A Randomized Double Blind Placebo Controlled Trial To Assess The Safety And Efficacy Of A Patented Fenugreek (Trigonella Foenum-Graecum) Seed Extract In Type 2 Diabetics, Rajinder Singh Gupta, Amarjit Singh Grover, Pawan Kumar, Apurva Goel, Samudra P. Banik, Sanjoy Chakraborty, Mehul Rungta, Manashi Bagchi, Partha Pal, Debasis Bagchi

Publications and Research

Background: Fenugreek plant (Trigonella foenum-graecum) constitutes a traditionally acclaimed herbal remedy for many human ailments including diabetes, obesity, neurodegenerative diseases, and reproductive disorders. It is also used as an effective anti-oxidative, anti-inflammatory, antibacterial, and anti-fungal agent. The seed of the plant is especially enriched in several bioactive molecules including polyphenols, saponins, alkaloids, and flavonoids and has demonstrated potential to act as an antidiabetic phytotherapeutic. A novel patented formulation (Fenfuro^®) was developed in our laboratory from the fenugreek seeds which contained >45% furostanolic saponins (HPLC).

Objective: A placebo-controlled clinical compliance study was designed to assess …

Cross-Linguistic Differences In Neural Encoding And Processing Of Stop Consonants: The Impact Of Language Experience On Attention Allocation, Aline Dos Santos Oliveira

Dissertations, Theses, and Capstone Projects

This study aims to examine the intricate relationship between language experience and the neural processing of stop consonant speech sounds. Previous research has shown minimal differences in amplitudes and latencies of cortical Auditory Evoked Potentials (AEPs) to speech sounds across listeners from various language backgrounds. It is only towards the later latencies (around the P2 peak at 200 ms), that there have been suggestions of cross-linguistic differences. In addition, a recent study, observed an AEP difference between monolinguals and bilinguals when processing speech, specifically an "Nd effect”. The Nd effect, which is an increased negativity of the AEP is proposed …

Modeling Hydroxide In Classical Proton Hopping Simulations And Amyloid Beta Membrane Pores, Ankita Dutta

Dissertations, Theses, and Capstone Projects

H3O+ and OH- ions display anomalously large diffusion coefficients, a property that is often attributed to their ability to perform structural diffusion which allow these ions to move through aqueous solutions by sequential proton transfers, also known as ‘Grotthuss hopping’. A rearrangement of the local solvation structure is known to be the prerequisite for proton transfer events via these ions. Studying this phenomenon using classical molecular dynamics simulations that do not allow bond rearrangements poses significant obstacles. Here we use a Monte Carlo augmented classical Molecular Dynamics method called the MOBHY algorithm which allows instantaneous changes in protonation states in …