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Articles 1051 - 1080 of 2361
Full-Text Articles in Life Sciences
Effects And Mechanisms Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Type 1 And Type 2 On The Permeability And Viability Of Porcine Endometrial Epithelial Cells, Dran Rukarcheep
Chulalongkorn University Theses and Dissertations (Chula ETD)
Both PRRSV types 1 and 2 revealed the sign of reproductive disorders associated with a lesion at implantation sites. Impairment of maternal glandular endometrium cell integrity and function by PRRSV infection may impact a proper nourishing fetus. This research was aimed to examine the effects of PRRSV type 1 and type 2 directly on the viability and barrier function of the endometrium using porcine glandular endometrial epithelial cell culture (PEG). The comparison of the route and the strain of PRRSV infection coinciding with their virulent in reproductive epithelia were considered. PEG cells isolated from the 4-6 months old PRRSV-free-herd gilts …
Potentials Of Ivabradine To Improve Cardiac Function In Dogs With Naturally Occurring, Asymptomatic Degenerative Mitral Valve Disease, Prapawadee Pirintr
Potentials Of Ivabradine To Improve Cardiac Function In Dogs With Naturally Occurring, Asymptomatic Degenerative Mitral Valve Disease, Prapawadee Pirintr
Chulalongkorn University Theses and Dissertations (Chula ETD)
The main hypothesis of the present study is that ivabradine (1.0 mg/kg, orally, twice daily) can reduce heart rate (HR), myocardial oxygen consumption (MVO2) and improve cardiac function in dogs with degenerative mitral valve disease (DMVD) partly due to a reduction of cardiomyocyte apoptosis. In order to test the hypothesis, this study was divided into three parts. The first part aimed to determine the appropriate single oral dose of ivabradine for reduction of HR and MVO2 as assessed by rate-pressure product (RPP= HR x systolic blood pressure). Once the appropriate dose was achieved, the second part was conducted to investigate …
Effects And Mechanisms Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Type 1 And Type 2 On The Permeability And Viability Of Porcine Endometrial Epithelial Cells, Dran Rukarcheep
Chulalongkorn University Theses and Dissertations (Chula ETD)
Both PRRSV types 1 and 2 revealed the sign of reproductive disorders associated with a lesion at implantation sites. Impairment of maternal glandular endometrium cell integrity and function by PRRSV infection may impact a proper nourishing fetus. This research was aimed to examine the effects of PRRSV type 1 and type 2 directly on the viability and barrier function of the endometrium using porcine glandular endometrial epithelial cell culture (PEG). The comparison of the route and the strain of PRRSV infection coinciding with their virulent in reproductive epithelia were considered. PEG cells isolated from the 4-6 months old PRRSV-free-herd gilts …
Potentials Of Ivabradine To Improve Cardiac Function In Dogs With Naturally Occurring, Asymptomatic Degenerative Mitral Valve Disease, Prapawadee Pirintr
Potentials Of Ivabradine To Improve Cardiac Function In Dogs With Naturally Occurring, Asymptomatic Degenerative Mitral Valve Disease, Prapawadee Pirintr
Chulalongkorn University Theses and Dissertations (Chula ETD)
The main hypothesis of the present study is that ivabradine (1.0 mg/kg, orally, twice daily) can reduce heart rate (HR), myocardial oxygen consumption (MVO2) and improve cardiac function in dogs with degenerative mitral valve disease (DMVD) partly due to a reduction of cardiomyocyte apoptosis. In order to test the hypothesis, this study was divided into three parts. The first part aimed to determine the appropriate single oral dose of ivabradine for reduction of HR and MVO2 as assessed by rate-pressure product (RPP= HR x systolic blood pressure). Once the appropriate dose was achieved, the second part was conducted to investigate …
Effects Of Short-Term Growth Hormone Administration On Plasma Leptin Levels In Normal And Diet-Induced Obesity Rats, Sutharinee Likitnukul
Effects Of Short-Term Growth Hormone Administration On Plasma Leptin Levels In Normal And Diet-Induced Obesity Rats, Sutharinee Likitnukul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Obesity has related to the alteration of hormonal profiles, i.e. growth hormone (GH), insulin and leptin. Because of the pleiotropic mechanisms of GH, it plays important roles both in the pathophysiological mechanisms and the therapeutic hormonal supplementation, which probably affected plasma leptin. The present research aimed firstly to demonstrate the effect of short-term GH administration on plasma leptin in 3 conditions; basal, meal-induced and fasting condition, from the rats with different adipose tissue mass. Secondly, to investigate short-term effect of GH on insulin sensitivity and body adiposity in control, diet resistant (DR) and diet-induced obesity (DIO) rats. In this regard, …
The Protective Effect Of Hydroxyxanthone On The Leakiness Of Intestinal Epithelia By The Proinflammatory Cytokine Related To Bacterial Endotoxin, Wannaporn Chayalak
The Protective Effect Of Hydroxyxanthone On The Leakiness Of Intestinal Epithelia By The Proinflammatory Cytokine Related To Bacterial Endotoxin, Wannaporn Chayalak
Chulalongkorn University Theses and Dissertations (Chula ETD)
Leaky gut characterized as decreased transepithelial electrical resistance and increased paracellular permeability to macromolecules is associated with several fatal diseases including sepsis. Pro-inflammatory cytokine IL-1β is a critical mediator of underlying mechanism through activation of myosin light chain kinase pathway. Blocking the inducible phosphorylated myosin light chain (p-MLC) indicates the successful treatment. Xanthones predominantly found in mangosteen (Garcinia mangostana) has been indicated as a potent anti-inflammatory action. Hydroxyxanthones (HDX), the major natural xanthones, have been recently synthesized in various forms. This study aimed to examine and screen the action of various forms of synthetic HDXs, in preventing the increased paracellular …
Expression And Characterization Of Α-Glucosidase From Weissella Confusa Bbk-1, Lalita Silapasom
Expression And Characterization Of Α-Glucosidase From Weissella Confusa Bbk-1, Lalita Silapasom
Chulalongkorn University Theses and Dissertations (Chula ETD)
Alpha-glucosidase [EC 3.2.1.20; α-D-glucoside glucohydrolase] is an exohydrolase which catalyzes non-reducing end of substrates to release D-glucose. In addition, alpha-glucosidase also displays a transferase activity, which bring about the formation of α-glucosylated compounds such as soluble starch and glycogen. This research aims to express and characterize α-glucosidase from Weissella confusa BBK-1 (WcAG). WcAG gene was expressed in modified pET28b vector. This gene contained an open reading frame of 1,775 bps. Optimum expression condition of WcAG was cultured in LB medium containing 1% (w/v) glucose and induced with 0.4 mM IPTG at 20°C for 20 h. The recombinant WcAG containing his-tag …
Synthesis Of Fisetin Glycosides By Cyclodextrin Glycosyltransferase From Paenibacillus Sp. Rb01, Nattawadee Lorthongpanich
Synthesis Of Fisetin Glycosides By Cyclodextrin Glycosyltransferase From Paenibacillus Sp. Rb01, Nattawadee Lorthongpanich
Chulalongkorn University Theses and Dissertations (Chula ETD)
Fisetin is a flavonoid which was widely exerted in pharmacological industries. However, fisetin has low water solubility and unstable in aqueous solution. So the uses of fisetin have been limited. The purpose of this study was to increase water solubility of fisetin by transglycosylation reaction using cyclodextrin glycosyltransferase (CGTase) from Paenibacillus sp. RB01. The reaction had to perform in co-organic solvent system and DMSO at 40% (v/v) was proved to be the most suitable co-organic solvent since it provided the high enzyme stability and high production yield. Moreover, the types of glycosyl donor were also determined and β-cyclodextrin was found …
Oxidative Responses In Transgenic 'Kdml105' Rice Oryza Sativa L. Overexpressing Oscam1-1 Against Drought Stress, Pun Sangchai
Oxidative Responses In Transgenic 'Kdml105' Rice Oryza Sativa L. Overexpressing Oscam1-1 Against Drought Stress, Pun Sangchai
Chulalongkorn University Theses and Dissertations (Chula ETD)
The OsCaM1-1 is a rice calmodulin protein which transduces Ca2+ signals to their target protein that is important to regulate cellular and physiological responses. Drought stress is the one of essential environmental stresses that affected the crop by changing in morphological, physiological, and biochemical direction tactics of plants. In this study, the effect of drought stress on growth and antioxidant system in the transgenic rice overexpressing OsCaM1-1 gene was investigated and compared to the control and wild type KDML 105. The overexpression of OsCaM1-1 in transgenic rice could increase plant tolerance to drought stress, the results showed the greater increase …
Cloning And Characterization Of Bacterial Mannanase Isolated From Dynastes Hercules Larvae, Sitipon Leerawatthanakun
Cloning And Characterization Of Bacterial Mannanase Isolated From Dynastes Hercules Larvae, Sitipon Leerawatthanakun
Chulalongkorn University Theses and Dissertations (Chula ETD)
Bacterial isolate HM7 was isolated from Dynastes hercules larvae excrement. It was identified as Bacillus sp. by 16S rRNA gene analysis. When the bacteria were cultured for β-mannanase production in medium containing konjac flour it had the highest specific activity of 138 U/mg at 36 h, and when cultured in medium containing coconut meal it had the highest specific of 114 U/mg at 48 h. The 1089 base pairs long of a β-mannanase encoding gene (Man26HM7) was successfully cloned and expressed in Escherichia coli BL21 (DE3), using pET21-b expression system. The enzyme was purified and characterized. The optimal condition MAN26HM7catalysis …
Isolation And Characterization Of Triclocarban Degrading Bacteria As Plant Growth Promoter, Merry Krisdawati Sipahutar
Isolation And Characterization Of Triclocarban Degrading Bacteria As Plant Growth Promoter, Merry Krisdawati Sipahutar
Chulalongkorn University Theses and Dissertations (Chula ETD)
Triclocarban (TCC) is a widely used as antimicrobial agent in household products. High usage volume of TCC results in its accumulation in sludge and lead to contamination in environments. Application of biosolid in agricultural activities leads to contamination of TCC in agricultural areas. So. development of bioremediation technique for TCC is necessary to maintain quality of agricultural ecosystem. For that purpose, this research isolated 5 soil bacteria capable of TCC degradation and with plant-growth promoting (PGP) activities including Ochrobactrum sp. MC22, Ochrobactrum sp. MC35, Sphingobacterium sp. MC43, Pseudomonas sp. MS45, and Pseudomonas fluorescens MC46. Among them, Ochrobactrum sp. MC22 and …
Development Of Molecular Technique For Risk Assessment And Risk Management Of Listeria Monocytogenes And L. Innocua In Frozen Cooked Chicken Plant, Chirapiphat Phraephaisarn
Development Of Molecular Technique For Risk Assessment And Risk Management Of Listeria Monocytogenes And L. Innocua In Frozen Cooked Chicken Plant, Chirapiphat Phraephaisarn
Chulalongkorn University Theses and Dissertations (Chula ETD)
Thailand is one of the largest cooked chicken meat exporting country in the world. Unfortunately, these products can be contaminated with L. monocytogenes causes the disease Listeriosis which carries a fatality rate of 30 to 40 percent. Therefore, the various importing countries including Japan and the European countries require a zero tolerance for L. monocytogenes in the cooked chicken products. Besides, all Listeria spp. occurrence, and not only L. monocytogenes, in these products is also unacceptable to the importers. Rejection of Listeria contaminated product can reach over 5 to 20 million baht per factory per year. Hence, the aim of …
Antiproliferative Activity Against Jurkat Cells Of Sahm1 Peptide Conjugated Gold Nanoparticles, Mukthida Thippayamonta
Antiproliferative Activity Against Jurkat Cells Of Sahm1 Peptide Conjugated Gold Nanoparticles, Mukthida Thippayamonta
Chulalongkorn University Theses and Dissertations (Chula ETD)
In general, treatments of cancer may involve medication, surgery, radiation and chemotherapy. Although these treatments are successful at a certain satisfactory level, their side effects are not acceptable for some patients. Thus, many researches have been investigated alternative methods to cure cancer. A technique of interest is the use of targeted drug therapy in which drug is designed to selectively kill only the cancer cells and to do no harm on the normal cells. Stapled α-helical peptide derived from mastermind-like I or SAHM1 is a small peptide which has been reported to have antiproliferative activity against cancer cells. However, its …
Separation And Purification Of Lactic Acid From Fermentation Broth By Reverse Osmosis, Natnirin Phanthumchinda
Separation And Purification Of Lactic Acid From Fermentation Broth By Reverse Osmosis, Natnirin Phanthumchinda
Chulalongkorn University Theses and Dissertations (Chula ETD)
Brackish water reverse osmosis (BWRO) and seawater reverse osmosis (SWRO) membranes were used in the 2-stage reverse osmosis (RO) unit to recover, pre-purify, and pre-concentrate lactic acid. Calcium lactate (CaLAC), sodium lactate (NaLAC), and ammonium lactate (NH4LAC) were used as model feed solutions. The operating pressure showed a pronounced effect on lactate passage through the first BWRO unit, and the Donnan exclusion effect and hydrogen bonding were responsible for cation rejection. Calcium ions were rejected at the BWRO unit because of low diffusion rate and charge interaction at the surface. However, monovalent ions formed hydrogen bonds with the carbonyl group …
Development Of Immunoassay On Paper-Based Microfluidic Devices For Determination Of Ractopamine, Alpha-Fetoprotein, Human Thyroid Stimulating Hormone, Pattarachaya Preechakasedkit
Development Of Immunoassay On Paper-Based Microfluidic Devices For Determination Of Ractopamine, Alpha-Fetoprotein, Human Thyroid Stimulating Hormone, Pattarachaya Preechakasedkit
Chulalongkorn University Theses and Dissertations (Chula ETD)
In this dissertation, immunoassays on paper-based microfluidic devices have been developed using different types of label for various applications in agriculture safety and medical diagnosis, which can be divided into three parts. In the first part, the conjugation method of ractopamine (RAC) and protein carrier is developed for fabricating paper-based lateral flow strip test of RAC using gold nanoparticles (AuNPs) as a label. The developed conjugation method provided a simple and one-step method with the use of less chemicals. The strip test for RAC determination in animal feed offers a linearity of 0.075-0.750 ng g-1 and a limit of detection …
Ammonium Removal And Biomass Production By Unicellular Cyanobacterium Synechococcus Sp., Piroonporn Srimongkol
Ammonium Removal And Biomass Production By Unicellular Cyanobacterium Synechococcus Sp., Piroonporn Srimongkol
Chulalongkorn University Theses and Dissertations (Chula ETD)
Using microalgae as a biofuel feedstock has played more attention over a decade. The cultivation of microalgae requires high water use and high initial investment that can make the process still not economically appealing. This study attempted to examine the potential for using brackish shrimp aquaculture effluents as culture medium for algal biomass production. To investigate whether algae could be used to remove ammonium from brackish shrimp aquaculture wastewater, marine cyanobacterium, Synechococcus sp. was cultured in BG-11 medium supplemented with Turks Island salt solution and different concentrations of NH4Cl (10-40 mg N L-1) for 18 days. The results showed that, …
Bioactive Peptide Derived From Trypsin Hydrolysis Of Unicellular Cyanobacterium Synechococcus Sp., Rutairat Suttisuwan
Bioactive Peptide Derived From Trypsin Hydrolysis Of Unicellular Cyanobacterium Synechococcus Sp., Rutairat Suttisuwan
Chulalongkorn University Theses and Dissertations (Chula ETD)
A free radicals can promote denaturing reactions in many cellular components, which create oxidative stress and it results to damage in body tissue. The free radicals can causing many diseases including heart diseases, Alzheimer, inflammation and cancer. This research aimed to investigate the effect of bioactive peptides derived from Synechococcus sp. cultured for 21 days on antioxidant, anti-inflammation and anticancer activities. The Synechococcus sp. protein hydrolysate was prepared by trypsin digest and purified by ultrafiltration with molecular weight cut off membranes of 10, 5 and 3 kDa. The MW <3 kDa fraction showed the highest NO, ABTS and DPPH radical scavenging activities with IC50 values of 34.51±9.8 µg protein/mL, IC50 11.54±0.3 µg protein/mL and IC50 13.63±0.15 µg protein/mL, respectively. The F2 fraction from gel filtration chromatography showed the strongest NO and ABTS radical scavenging activities with IC50 values of 7.68±0.64 µg protein/mL and 9.74±0.2 µg protein/mL, respectively. The F2 was purified by RP-HPLC to yield four fractions. The 30 – 40 min sub-fraction (F2-4) was selected for further analysis by mass spectroscopy. Five isolated peptides with amino acid sequences of AILQSYSAGKTK; 1,265.69 Da, ALNKTHLIQTK; 1,265.74 Da, LLVHAPVK; 875.55 Da, IPDAHPVK; 875.48 Da and VVVLRDGAVQQLGTPR; 1,706.97 Da were identified. The F2-4 had higher DPPH and NO radical scavenging activity compared to the synthetic peptide. Moreover, AILQSYSAGKTK had the highest ABTS radical scavenging activity. Furthermore, the F2 fraction protected oxidation-induced DNA damage in pBR322, pKS and pUC19 cells. F2 fraction was selected to study anti-inflammatory and anticancer properties. Anti-inflammatory effect, F2 fraction showed no cytotoxicity toward RAW264.7 macrophage cells. RT-PCR and qRT-PCR results showed that F2 reduced gene expression of pro-inflammatory cytokines iNOS, TNF-α, COX-1, COX-2 and IL-6. For anticancer activity, F2 fraction showed high anticancer activities in the human colon cancer cells (SW620) according to cytotoxic activity (MTT assay), with IC50 values of 106.58±21.46 µg protein/mL. The F2 fraction activated the apoptotic pathway in SW620 cells after treatment for 24, 48 and 72 hours. The highest activities of caspases 3, 8 and 9 were observed after treatment for 72 hours. These findings suggested that unicellular cyanobacterium Synechococcus sp. may be used to develop for natural anti-inflammation and natural anticancer drugs.
Adaptation Of Terrilactibacillus Laevilacticus Sk5-6 For Growth And D-Lactic Acid Production In A Low Yeast Extract Concentration Medium, Srettapat Limsampancharoen
Adaptation Of Terrilactibacillus Laevilacticus Sk5-6 For Growth And D-Lactic Acid Production In A Low Yeast Extract Concentration Medium, Srettapat Limsampancharoen
Chulalongkorn University Theses and Dissertations (Chula ETD)
Lactic acid has been extensively used in food, personal care, and pharmaceutical products. Besides, lactic acid can be used as the sole feedstock in the synthesis of polylactic acid (PLA), the biodegradable plastic. The demand of the bioplastics including PLA has been increased due to the environmental impact of petroleum based plastic; therefore, this expedited the market demand of lactic acid. It should be noted that the polymer grade lactic acid is unlike those commercially available products for food and pharmaceutical applications. The high purity of stereoisomer either D-lactate or L-lactate is mandatory in PLA polymerization. In addition, the production …
Tyrosinase Inhibitors From Bark Of Talawrinta (Manilkara Kauki (L.) Dubard, Sirinada Srisupap
Tyrosinase Inhibitors From Bark Of Talawrinta (Manilkara Kauki (L.) Dubard, Sirinada Srisupap
Chulalongkorn University Theses and Dissertations (Chula ETD)
Melanin is essential for protecting human skin from ultraviolet radiation. However, its overproduction in the basal epithelial layer leads to hyperpigmentary disorders of the skin such as melasma, blemish and age spots. Tyrosinase is one of key enzymes in melanogenesis. Manilkara kauki (L.) Dubard or Talawrinta is one of the plants in genus Manilkara of Sapotaceae family. This study attempted to investigate in vitro total phenolic and flavonoid contents, antioxidant and tyrosinase inhibitory activities of crude extract of different parts of M. kauki and purify tyrosinase inhibitors from stem barks of M. kauki. Methanol and aqueous crude extracts of fruits, …
Bioactive Compounds From Songkhla Province Mangrove-Derived Endophytic Fungi And From Heart-Leaved Moonseed Tinospora Baenzigeri Forman, Sujitra Hanthanong
Bioactive Compounds From Songkhla Province Mangrove-Derived Endophytic Fungi And From Heart-Leaved Moonseed Tinospora Baenzigeri Forman, Sujitra Hanthanong
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study has evaluated biological activities of isolated compounds from Tinospora baenzigeri stems collecting from two different areas, and from mangrove-derived endophytic fungi isolated from trees inhabiting in Songkhla province. Four new rearranged clerodane-type diterpenes (66-69), one new glycoside (70), and six known compounds (61, 71-75), were extracted from plant obtained from local market in Bangkok. Twelve compounds including a new rearranged clerodane-type diterpene (76), a new rearranged clerodane glycoside (77), five known compounds (44, 78-81), and five compounds counting 61, 66, 70 and 74-75 were obtained from plant collected in Bueng Kan Province. Structures of new compounds 66-69 and …
Bioactive Compounds With An Inhibitory Activity Against Osteoclast Differentiation And Mechanisms, Supatta Chawalitpong
Bioactive Compounds With An Inhibitory Activity Against Osteoclast Differentiation And Mechanisms, Supatta Chawalitpong
Chulalongkorn University Theses and Dissertations (Chula ETD)
โรคกระดูกพรุนเป็นปัญหาสุขภาพที่สำคัญอย่างหนึ่งของสังคมผู้สูงอายุ สาเหตุหลักของภาวะดังกล่าวเกิดจากความไม่สมดุลของเซลล์ที่ทำหน้าทีสร้างกระดูก (osteoblast) และเซลล์ที่ทำหน้าที่สลายกระดูก (osteoclast) การลดลงของมวลกระดูกในผู้สูงอายุนำมาซึ่งความเสี่ยงในการแตกหักของกระดูก จุดประสงค์ของการศึกษาครั้งนี้ คือ การคัดกรองสารออกฤทธิ์ทางชีวภาพที่มีฤทธิ์ในการต้านการเปลี่ยนสภาพของเซลล์สลายกระดูกและกลไกการออกฤทธิ์ของสารนั้นๆ ในกลุ่มสารทดสอบอนุพันธ์สาร (3S)-1-(3,4-dihydroxyphenyl)-3-hydroxy-7-phenyl-(6E)-6-heptene (DHPH) และ กรดไซเปอเรโนอิก (Cyperenoic acid) ซึ่งสกัดได้จากส่วนเหง้า Curcuma comosa Rox. และราก Croton crassifolius Geiseler. ตามลำดับ มีฤทธิ์แรงในการต้านการเกิดเซลล์สลายกระดูก ในระดับ in vitro ใช้เซลล์จากไขกระดูกที่ถูกกระตุ้นด้วย receptor activator of nuclear factor-κB ligand (RANKL) DHPH มีฤทธิ์ในการยับยั้งการเปลี่ยนสภาพไปเป็นเซลล์สลายกระดูก โดยยับยั้งการเกิด TRAP-positive multinucleated cells (TRAP+ MNCs) ที่ระดับความเข้มข้นยับยั้งกึ่งหนึ่ง (IC50) 325±1.37 นาโนโมลาร์ การยับยั้งดังกล่าวสอดคล้องกับการลดต่ำลงของสัญญาณภายใต้การกระตุ้นของ RANKL โดยรวมถึงวิถี Mitogen-activated protein kinases (MAPKs) เช่น ERK (p44/42) และ p38 แต่ไม่มีผลต่อวิถี NF-κB โดยผลของสารดังกล่าวยังส่งผลกระทบต่อระดับของ transcription factor เช่น nuclear factor of activation T cells (NFATc1) และ c-Fos ซึ่งมีความสำคัญมากในการเปลี่ยนสภาพเป็นเซลล์สลายกระดูก DHPH ลดการกัดกร่อนกระดูก (bone resorption) บนแผ่นกระดูก (bone slices) นอกจากนั้นสาร DHPH ยังสามารถเพิ่มกิจกรรมของเอนไซม์ alkaline phosphatase (ALP) ที่เกี่ยวข้องกับการเปลี่ยนสภาพของเซลล์สร้างกระดูกในเซลล์ชนิด MC3T3-E1 ในกรณีของกรดไซเปอเรโนอิกนั้น พบว่าสามารถลดการเกิด TRAP+ MNCs ที่ระดับความเข้มข้น IC50 ที่ 36.69±1.02 …
Tyrosinase Inhibitors From Sapodilla Plum Manilkara Zapota L., Sutthiduean Chunhakant
Tyrosinase Inhibitors From Sapodilla Plum Manilkara Zapota L., Sutthiduean Chunhakant
Chulalongkorn University Theses and Dissertations (Chula ETD)
Six different parts of Manilkara zapota which consisted of barks, flowers, fruits, leaves, roots and seeds were investigated for total phenolic content, total flavonoid content, antioxidant and antityrosinase activities. Methanol crude extract of flowers showed the highest total phenolic content (368.73 ± 0.65 mg GAE/g), while methanol crude extracts of seeds and roots showed high total flavonoid content (90.21 ± 0.57 and 89.03 ± 1.00 mg QE/g, respectively). Methanol crude extract of seeds showed the strongest DPPH (IC50 282.05 ± 0.60 μg/mL) and ABTS (IC50 205.11 ± 0.89 μg/mL) radical scavenging activities and showed the highest FRAP value of 296.46 …
Diversity Of Mosses In Phu Kradueng National Park, Loei Province, Patsakorn Ajintaiyasil
Diversity Of Mosses In Phu Kradueng National Park, Loei Province, Patsakorn Ajintaiyasil
Chulalongkorn University Theses and Dissertations (Chula ETD)
The study of mosses diversity in Phu Kradueng National Park, Loei Province was explored from December 2014 to December 2016. The samples were collected along the 6 natural trails. A total of 501 samples were identified into 30 families, 55 genera, 100 species, 5 subspecies, and 9 varieties. Three families of mosses namely Sphagnaceae, Fissidentaceae, and Leucobryaceae were common families which included 11, 9 and 7 taxa, respectively. Additionally, the most common microhabitat was saxicolous with 45 species. The second and third common microhabitats were terrestrial and corticolous with 43 and 37 species, respectively. Additionally, there are 4 species which …
Effects Of Plant Extracts On Melanin Biosynthesis And Melanogenetic Gene Expression In B16f10 Melanoma Cells, Moragot Chatatikun
Effects Of Plant Extracts On Melanin Biosynthesis And Melanogenetic Gene Expression In B16f10 Melanoma Cells, Moragot Chatatikun
Chulalongkorn University Theses and Dissertations (Chula ETD)
Ultraviolet radiation from sunlight is a significant environmental factor in skin damage and can induce hyperpigmentation disorder and aesthetic problem. Development of novel whitening phytochemical compounds from natural products has been become trends recently. The purpose of this study was to find some plant extracts that reduce melanin synthesis and melanogenetic gene expression in alpha-MSH-induced B16F10 mouse melanoma cells. To screen total phenolics and flavonoids, antioxidant activity, and anti-mushroom tyrosinase activity, we used 13 plants which were extracted with petroleum ether, dichloromethane and ethanol solvents, subsequently. We found that total phenolic content of 13 plants extracts was found in the …
Mechanisms Of Phytochemicals From Murraya Koenigii And Stevia Rebuadiana In Cell Model Of Diabetes, Hepatocellular Carcinoma And Cholangiocarcinoma Under Hyperglycemic Conditions, Nattakarn Nooron
Chulalongkorn University Theses and Dissertations (Chula ETD)
Diabetes mellitus (DM) is a group of metabolic disorders in which high blood glucose levels over a prolonged period, caused by deficiency of insulin production from the pancreas, or by the insulin resistance. Several epidemiological studies suggest that diabetic population is not only at increased risk of cardiovascular complications, but also at substantially higher risk of many types of malignancies. Hyperglycemia induced free radical reactive oxygen species (ROS) production, which is a major cause of cell injury and organ damage, especially affects pancreatic beta cells. Whereas insulin resistance in skeletal muscle tissue and adipocyte are the major sites of postprandial …
Role Of Progesterone Receptor A And B Isoforms In Lung Neuroendocrine Tumors, Teeranut Asavasupreechar
Role Of Progesterone Receptor A And B Isoforms In Lung Neuroendocrine Tumors, Teeranut Asavasupreechar
Chulalongkorn University Theses and Dissertations (Chula ETD)
Lung neuroendocrine tumors (NETs) incidences are increasing in recent years. Women with lung NETs have significantly better survival rates as compared to men suggesting the involvement of sex steroids and their receptors in the progression of lung NETs. Recent data suggested that progesterone receptor (PR) may play a role in the survival of lung NET patients. PR exists as two major isoforms, PRA and PRB. How expression of PR isoforms affects proliferation of lung NETs and patient's survival is not known. To determine the role of PR isoforms in lung NETs, we constructed H727 lung NET cell models expressing PRB …
Bioactive Clitoria Ternatea L. Flower Extract For The Glycemic Control To Carbohydrate Foods And Sugary Beverages, Charoonsri Chusak
Bioactive Clitoria Ternatea L. Flower Extract For The Glycemic Control To Carbohydrate Foods And Sugary Beverages, Charoonsri Chusak
Chulalongkorn University Theses and Dissertations (Chula ETD)
Clitoria ternatea L. flower, an edible plant containing anthocyanins, has been currently used as the source of natural colorant in foods, beverages and desserts worldwide. The previous report demonstrated its biological properties including antioxidant and antihyperglycemic activity. However, the various concentrations of Clitoria ternatea were used in food and beverages but the effective concentration of Clitoria ternatea on the digestibility of starch and glycemic response remains unknown. In this study, the effects of foods and beverage containing Clitoria ternatea L. flower extract (CTE) on starch digestibility in vitro, postprandial glycemic response and antioxidant capacity in human as well as sensory …
Stability And Bioactivities Of Mesona Chiensis Benth Extract Microbeads Under Simulated Gastrointestinal Digestion, Chonnipa Wongverawattanakul
Stability And Bioactivities Of Mesona Chiensis Benth Extract Microbeads Under Simulated Gastrointestinal Digestion, Chonnipa Wongverawattanakul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Alginate-calcium microencapsulation of Mesona chinensis extract (MCE) was conducted in this study. Firstly, the MCE was encapsulated by using calcium chloride (3 and 5% w/v) and sodium alginate (1.5 and 1.8% w/v) to form MC microbeads (MCB). The MCB with condition of 1.5% w/v sodium alginate, 3% w/v calcium chloride and 75% w/v MCE demonstrated the highest %encapsulation efficiency and suitable spherical shape among other conditions. According to Fourier-transform infrared spectroscopy (FT-IR), there was no strong chemical interaction between alginate and MCE. After simulated digestion, the total phenolic content (TPC) and antioxidant activity measured by ferric reducing antioxidant power (FRAP) …
The Effect Of Nutrition Education Intervention On The Amount Of School Lunch Consumption In Children Aged 3-5 Years: Case Study In Bangkok, Thailand, Hathaichanok Tirapongporn
The Effect Of Nutrition Education Intervention On The Amount Of School Lunch Consumption In Children Aged 3-5 Years: Case Study In Bangkok, Thailand, Hathaichanok Tirapongporn
Chulalongkorn University Theses and Dissertations (Chula ETD)
Low vegetable and fruit intake and micronutrient deficiency has been reported for Thai children aged three to five years. Many nutrition education programs have been recommended to solve these problems, especially for preschool children. This study aims to investigate the effect of the novel nutrition education program on the amount of vegetables, fruit, energy and nutrients consumption in school lunches of children aged three to five years. United for Healthier Kids is a nutrition education program that desires to motivate healthy eating via a set of nutrition education tools by encouraging variety in the types of food consumed, increase vegetables …
Effect Of Different Cooking Methods On The Bioaccessibility Of Polyphenols And Antioxidant Capacities Of Pakwan Ban (Sauropus Androgynus), Imas Arumsari
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study was aimed to assess the effect of different cooking methods on the amounts of phytochemical compounds and antioxidant capacities of sweet leaf (Sauropus androgynus) before and during in vitro simulated gastrointestinal digestion. The optimum cooking time of each cooking method was prior selected according to the total phenolic compounds, the ferric-reductase antioxidant power (FRAP) activity, the color characteristics, the hardness, and the texture preference of cooked sweet leaf samples. Consequently, the sweet leaf samples cooked by boiling for 5 mins or microwaving at 800 watts for 90 sec were compared with raw sweet leaf. The results showed that …