Life Sciences Commons^™

Integrative Genomic And Transcriptomic Analysis For Pinpointing Recurrent Alterations Of Plant Homeodomain Genes And Their Clinical Significance In Breast Cancer, Huimei Yu, Yuanyuan Jiang, Lanxin Liu, Wenqi Shan, Xiaofang Chu, Zhe Yang, Zeng-Quan Yang

Oncology Faculty Publications

A wide range of the epigenetic effectors that regulate chromatin modification, gene expression, genomic stability, and DNA repair contain structurally conserved domains called plant homeodomain (PHD) fingers. Alternations of several PHD finger-containing proteins (PHFs) due to genomic amplification, mutations, deletions, and translocations have been linked directly to various types of cancer. However, little is known about the genomic landscape and the clinical significance of PHFs in breast cancer. Hence, we performed a large-scale genomic and transcriptomic analysis of 98 PHF genes in breast cancer using TCGA and METABRIC datasets and correlated the recurrent alterations with clinicopathological features and survival of …

Evolution In The Deep Sea: Scales And Mechanisms Of Population Divergence, Amanda E. Glazier

Graduate Doctoral Dissertations

The deep sea is the Earth’s largest ecosystem and harbors a unique and largely endemic fauna. Although most research has focused on the ecological mechanisms that allow coexistence, recent studies have begun to investigate how this remarkable fauna evolved.. My work quantifies geographic patterns of genetic variation and investigates potential mechanisms that shape evolution in the deep ocean.

Bathymetric genetic divergence is common in the deep sea with population structure typically decreasing with depth. The evolutionary mechanisms that underlie these patterns are poorly understood. Geographic patterns of genetic variation indicated that the protobranch bivalve Neilonella salicensis was composed of two …

Minibrain And Wings Apart Control Organ Growth And Tissue Patterning Through Downregulation Of Capicua, Liu Yang

Graduate Doctoral Dissertations

The regulation of organ growth is a fundamental aspect of developmental biology. My work uses Drosophila as a model system to understand how the various growth regulators are coordinated. The transcriptional repressor Capicua (Cic) controls tissue patterning and restricts organ growth, and has been recently implicated in several cancers and neurodegenerative diseases. Cic has emerged as a primary sensor of signaling downstream of the receptor tyrosine kinase (RTK)/extracellular signal-regulated kinase (ERK) pathway, but how Cic activity is regulated in different cellular contexts remains poorly understood. In order to identify Cic regulators, I have used affinity purification/mass spectrometry (AP-MS) to study …

Pengaruh Latihan Imagery Dan Koordinasi Terhadap Keterampilan Forehand Drive Petenis Pemula, Risti Nurfadhila

Jurnal Keolahragaan

Penelitian ini bertujuan untuk mengetahui: (1) perbedaan pengaruh antara latihan imagery dengan menggunakan script dan video terhadap keterampilan forehand drive petenis pemula, (2) perbedaan keterampilan forehand drive antara petenis pemula yang memiliki koordinasi tinggi dan koordinasi rendah, (3) interaksi antara latihan imagery dan koordinasi terhadap keterampilan forehand drive petenis pemula. Metode penelitian adalah eksperimen dengan rancangan faktorial 2 x 2. Sampel penelitian adalah 32 orang petenis pemula mahasiswa PJKR FIK UNY yang mengambil mata kuliah olahraga pilihan tenis yang diambil dengan teknik Purposive Random Sampling. Seluruh data penelitian diperoleh melalui tes dan pengukuran terhadap koordinasi dengan menggunakan tes koordinasi …

Pengaruh Metode Latihan Dan Persepsi Kinestetik Terhadap Keterampilan Groundstrokes Tenis Lapangan Pada Siswa Sd, Suryono Suryono

Jurnal Keolahragaan

Penelitian ini bertujuan untuk mengetahui: (1) Perbedaan pengaruh antara metode demonstrasi dan metode audio visual terhadap keterampilan groundstrokes tenis lapangan, (2) Perbedaan pengaruh antara persepsi kinestetik terhadap keterampilan groundstrokes tenis lapangan, dan (3) interaksi metode latihan dan persepsi kinestetik terhadap keterampilan groundstrokes. Penelitian ini merupakan penelitian eksperimen. Populasi penelitian adalah siswa SD Negeri Petinggen dan SD Negeri Terbansari yang berjumlah 75 siswa. Sampel penelitian 40 siswa ditentukan dengan teknik purposive sampling. Pengumpulan data menggunakan tes persepsi kinestetik dan keterampilan groundstrokes. Instrumen dalam penelitian ini adalah horizontal liner space test dan memantulakan bola ke dinding. Teknik analisis data …

Pengaruh Metode Uchikomi Dan Flexibility Terhadap Hasil Bantingan Pada Pejudo Daerah Istimewa Yogyakarta, Suwarli Suwarli

Jurnal Keolahragaan

Penelitian bertujuan mengetahui: (1) perbedaan pengaruh antara metode uchikomi dengan menggunakan karet ban sepeda dan uchikomi jalan terhadap hasil bantingan judo, (2) perbedaan hasil bantingan antara pejudo yang memiliki flexibility tinggi dan flexibility rendah, dan (3) interaksi antara metode uchikomi dan flexibility terhadap hasil bantingan judo. Metode penelitian adalah eksperimen dengan rancangan faktorial 2 x 2. Populasi penelitian adalah pejudo D.I. Yogyakarta yang berjumlah 44 pejudo. Sampel penelitian ini 24 pejudo yang diambil dengan teknik Purposive Rondom Sampling. Data penelitian ini diperoleh melalui tes dan pengukuran. Instrumen penelitian adalah sit and reach dan Randori (dipertandingkan) selama tiga menit. Teknik …

Pengaruh Latihan Beban Dan Kekuatan Otot Terhadap Hypertrophy Otot Dan Ketebalan Lemak, Eko Sucipto, Widiyanto Widiyanto

Jurnal Keolahragaan

Penelitian ini bertujuan untuk mengetahui: (1) perbedaan pengaruh antara latihan beban metode compound set dan circuit training, (2) perbedaan pengaruh kekuatan otot tinggi dan rendah,dan (3) interaksi antara metode latihan beban dan kekuatan terhadap hasil hypertrophy otot dan ketebalan lemak. Populasi dalam penelitian ini adalah 56 members yang programnya hypertrophy otot dari Fitness Centre GOR FIK UNY dan Fitness Centre Club Arena Hotel Jayakarta Yogyakarta. Sampel yang berjumlah 28 members ditentukan dengan teknik Purposive Rondom Sampling. Teknik analisis data yang digunakan dalam penelitian ini adalah Analisis Varian (ANAVA). Hasil penelitian adalah: (1) ada perbedaan pengaruh yang signifikan antara …

Variants In Wfs1 And Other Mendelian Deafness Genes Are Associated With Cisplatin-Associated Ototoxicity, Heather E. Wheeler, Eric R. Gamazon, Robert Frisina, Carlos Perez-Cervantes, Omar El Charif, Brandon Mapes, Sophie D. Fossa, Darren Feldman, Robert Hamilton, David J. Vaughn, Clair Beard, Chunkit Fung, Chiristian Kollmannsberger, Jeri Kim, Taisei Mushiroda, Michiaki Kubo, Shirin Ardeshir-Rouhani-Fard, Lawrence H. Einhorn, Nancy Cox, M. Eileen Dolan, Lois Travis

Bioinformatics Faculty Publications

Cisplatin is one of the most commonly used chemotherapy drugs worldwide and one of the most ototoxic. We sought to identify genetic variants that modulate cisplatin-associated ototoxicity (CAO). Experimental Design: We performed a genome-wide association study (GWAS) of CAO using quantitative audiometry (4-12 kHz) in 511 testicular cancer survivors of European genetic ancestry. We performed polygenic modeling and functional analyses using a variety of publicly available databases. We used an electronic health record cohort to replicate our top mechanistic finding. Results: One SNP, rs62283056, in the first intron of Mendelian deafness gene WFS1 (wolframin ER transmembrane glycoprotein) and an expression …

Evolutionary Dynamics Of Tree Invasions: Complementing The Unified Framework For Biological Invasions, Rafael D. Zenni, Ian A. Dickie, Michael J. Wingfield, Heidi Hirsch, Casparus J. Crous, Laura A. Meyerson, Treena I. Burgess, Thalita G. Zimmermann, Metha M. Klock, Evan Siemann, Alexandra Erfmeier, Roxana Aragon, Lia Montti, Johannes J. Le Roux

Faculty Publications, Environmental Studies

Evolutionary processes greatly impact the outcomes of biological invasions. An extensive body of research suggests that invasive populations often undergo phenotypic and ecological divergence from their native sources. Evolution also operates at different and distinct stages during the invasion process. Thus, it is important to incorporate evolutionary change into frameworks of biological invasions because it allows us to conceptualize how these processes may facilitate or hinder invasion success. Here, we review such processes, with an emphasis on tree invasions, and place them in the context of the unified framework for biological invasions. The processes and mechanisms described are pre-introduction evolutionary …

Stability Of Cross-Feeding Polymorphisms In Microbial Communities, Ivana Gudelj, Margie Kinnersley, Peter Rashkov, Karen Schmidt, Frank Rosenzweig

Biological Sciences Faculty Publications

Cross-feeding, a relationship wherein one organism consumes metabolites excreted by another, is a ubiquitous feature of natural and clinically-relevant microbial communities and could be a key factor promoting diversity in extreme and/or nutrient-poor environments. However, it remains unclear how readily cross-feeding interactions form, and therefore our ability to predict their emergence is limited. In this paper we developed a mathematical model parameterized using data from the biochemistry and ecology of an E. coli cross-feeding laboratory system. The model accurately captures short-term dynamics of the two competitors that have been observed empirically and we use it to systematically explore the stability …

Winner's Curse Correction And Variable Thresholding Improve Performance Of Polygenic Risk Modeling Based On Genome-Wide Association Study Summary-Level Data, Jianxin Shi, Ju-Hyun Park, Jubao Duan, Sonja T. Berndt, Winton Moy, Kai Yu, Lei Song, William Wheeler, Xing Hua, Debra Silverman, Montserrat Garcia-Closas, Chao Agnes Hsiung, Jonine D. Figueroa, Victoria K. Cortessis, Nuria Malats, Margaret R. Karagas

Dartmouth Scholarship

Recent heritability analyses have indicated that genome-wide association studies (GWAS) have the potential to improve genetic risk prediction for complex diseases based on polygenic risk score (PRS), a simple modelling technique that can be implemented using summary-level data from the discovery samples. We herein propose modifications to improve the performance of PRS. We introduce threshold-dependent winner’s-curse adjustments for marginal association coefficients that are used to weight the single-nucleotide polymorphisms (SNPs) in PRS. Further, as a way to incorporate external functional/annotation knowledge that could identify subsets of SNPs highly enriched for associations, we propose variable thresholds for SNPs selection. We applied …

Influence Of Maternal Breast Milk Ingestion On Acquisition Of The Intestinal Microbiome In Preterm Infants., Katherine E Gregory, Buck S Samuel, Pearl Houghteling, Guru Shan, Frederick M Ausubel, Ruslan I Sadreyev, W Allan Walker

Faculty Publications

BACKGROUND: The initial acquisition and early development of the intestinal microbiome during infancy are important to human health across the lifespan. Mode of birth, antibiotic administration, environment of care, and nutrition have all been shown to play a role in the assembly of the intestinal microbiome during early life. For preterm infants, who are disproportionately at risk of inflammatory intestinal disease (i.e., necrotizing enterocolitis), a unique set of clinical factors influence the establishment of the microbiome. The purpose of this study was to establish the influence of nutritional exposures on the intestinal microbiome in a cohort of preterm infants early …

Analyzing And Modeling The Dysfunction Of Inhibitory Neurons In Alzheimer’S Disease, Carlos Perez, Jokubas Ziburkus, Ghamim Ullah

Physics Faculty Publications

Alzheimer’s disease (AD) is characterized by the abnormal proteolytic processing of amyloid precursor protein, resulting in increased production of a self-aggregating form of beta amyloid (Aβ). Several lines of work on AD patients and transgenic mice with high Aβ levels exhibit altered rhythmicity, aberrant neuronal network activity and hyperexcitability reflected in clusters of hyperactive neurons, and spontaneous epileptic activity. Recent studies highlight that abnormal accumulation of Aβ changes intrinsic properties of inhibitory neurons, which is one of the main reasons underlying the impaired network activity. However, specific cellular mechanisms leading to interneuronal dysfunction are not completely …

Cullin-4 Regulates Wingless And Jnk Signaling-Mediated Cell Death In The Drosophila Eye., Meghana Tare, Ankita Sarkar, Shimpi Bedi, Madhuri Kango-Singh, Amit Singh

Biology Faculty Publications

In all multicellular organisms, the fundamental processes of cell proliferation and cell death are crucial for growth regulation during organogenesis. Strict regulation of cell death is important to maintain tissue homeostasis by affecting processes like regulation of cell number, and elimination of unwanted/unfit cells. The developing Drosophila eye is a versatile model to study patterning and growth, where complex signaling pathways regulate growth and cell survival. However, the molecular mechanisms underlying regulation of these processes is not fully understood. In a gain-of-function screen, we found that misexpression of cullin-4 (cul-4), an ubiquitin ligase, can rescue reduced eye mutant phenotypes. Previously, …

Exploring Knowledge, Beliefs And Practices Of Radon Gas Exposure Among Public Health Workers, Paschal Nwako

Seton Hall University Dissertations and Theses (ETDs)

Radon gas exposure is the highest cause of lung cancer among people that never smoked in the United States. People exposed to elevated levels of radon had a higher risk of developing lung cancer. Achieving the long term goal of the Indoor Radon Abatement Act (IRAA) of 1988 require a combined efforts of government agencies. Public health workers are change agents and their role in protecting and improving the health of their communities are well documented. This study created and utilized a multi question survey, the Public Health Workers Radon Assessment Instrument (PHWRAI) to explore knowledge, beliefs, personal and professional …

Connecting Culturally And Spiritually To Healthy Eating: A Community Assessment With Native Hawaiians, Mary Frances Oneha, Joan Dodgson, Mabel Ho`Oipo Decambra, Carol Titcomb, Rachelle Enos, Sandie Morimoto-Ching

Asian/Pacific Island Nursing Journal

Many of the chronic illnesses disproportionately experienced by Native Hawaiians are directly related to poor diets and long-standing obesity beginning in childhood. We report on the findings of in-depth key informant interviews (N = 14) that took place in two Native Hawaiian communities as part of a larger, community-based participatory research study that included a community assessment through individual interviews and focused group discussions, and a pilot intervention targeting pregnant women, their infants, and families. Four categories emerged from the qualitative analysis of interview transcripts that described an understanding of “healthy eating”: family roles and responsibilities, aspects of community …

Expansion Of The Molecular And Morphological Diversity Of Acanthamoebidae (Centramoebida, Amoebozoa) And Identification Of A Novel Life Cycle Type Within The Group, Alexander Tice, Lora Shadwick, Anna Maria Fiore-Donno, Stefen Geisen, Seungho Kang, Gabriel Schuler, Frederick Spiegel, Katherine Wilkinson, Michael Bonkowski, Kenneth Dumack, Daniel Lahr, Eckhard Voelcker, Steffen Claub, Junling Zhang, Matthew Brown

Faculty Publications, Biological Sciences

Background Acanthamoebidae is a “family” level amoebozoan group composed of the genera Acanthamoeba, Protacanthamoeba, and very recently Luapeleamoeba. This clade of amoebozoans has received considerable attention from the broader scientific community as Acanthamoeba spp. represent both model organisms and human pathogens. While the classical composition of the group (Acanthamoeba + Protacanthamoeba) has been well accepted due to the morphological and ultrastructural similarities of its members, the Acanthamoebidae has never been highly statistically supported in single gene phylogenetic reconstructions of Amoebozoa either by maximum likelihood (ML) or Bayesian analyses. Results Here we show using a phylogenomic approach that the Acanthamoebidae is …

Recent Diatoms Reported From The Central United States: Register Of Taxa And Synonyms, Mark E. Eberle

Biological Sciences Faculty Publications

This list of diatoms summarizes information for more than 1000 taxa and synonyms reported in published accounts of collections made in the central United States, principally within the states of Kansas, Nebraska, and Oklahoma, but also including adjacent areas in eastern Colorado and western Missouri. The objective was to provide people working on diatom projects in this region with a base reference to help them assess the results of their research. Records from Master’s theses and references focused on fossil diatoms were not incorporated into this list. Specimens were not examined, so taxa presented here are those reported in the …

The Replication Initiator Of The Cholera Pathogen’S Second Chromosome Shows Structural Similarity To Plasmid Initiators, Natalia Orlova, Matthew Gerding, Olha Ivashkiv, Paul Dominic B. Olinares, Brian T. Chait, Matthew K. Waldor, David Jeruzalmi

Publications and Research

The conserved DnaA-oriC system is used to initiate replication of primary chromosomes throughout the bacterial kingdom; however, bacteria with multipartite genomes evolved distinct systems to initiate replication of secondary chromosomes. In the cholera pathogen, Vibrio cholerae, and in related species, secondary chromosome replication requires the RctB initiator protein. Here, we show that RctB consists of four domains. The structure of its central two domains resembles that of several plasmid replication initiators. RctB contains at least three DNA binding winged-helix-turn-helix motifs, and mutations within any of these severely compromise biological activity. In the structure, RctB adopts a headto- head dimeric configuration …

The Effects Of Operating Parameters Of Cigarette Making Machine On Cigarette Yield, Yao Ermin, Shao Ning, Li Xiao, Jiang Xuebing, Zhang Mingya, Xu Xiufeng

Food and Machinery

The effects of cigarette machine operating parameters were studied on the yield of different grades of cigarettes. The effects of operating parameters, such as the amount of running silk, yarn feeding and yarn supply and the volume of ticking, on the yield of cigarettes A, B and C were studied by uniform design method. The results were as follows. ① The polynomial models of the running parameters and yield of different grades cigarettes were established. for A grades, it was Y_A=27.500+0.704X₂+1.72E-03X^2.08₁-2.110E-03X₁X₃; for B grades, it was Y_B= …

Tomato Maturity Color Discrimination Based On Machine Vision, Bi Zhijian, Zhang Ruoyu, Qi Yanjie, Wu Yebei

Food and Machinery

Tomato quality is one of the most important factors ensured the consistency of tomato market factors. A color analysis method was proposed for classifying the fresh tomato, with reference to the national standard GB 8852—88, defining the classification standards of tomato maturity. In this study, tomato was divided into the four categories, full ripe, ripe, half ripe, and green ripe. RGB images of tomato were collected, removing the background and filtering de-noising, and then they were converted to HIS and HSV color models. Through the MATLAB programming, the mean values of the color components R, G, B, H, S, V, …

Identification Of A Strain Of High Yieldacetic Acid Bacteria From Ferment And Its Acid-Producing Characteristics, Lu Mengyao, Liu Shuliang, Hu Kaidi, Zhang Aiqing

Food and Machinery

A strain of high yield acetic acid bacteria LMY-1 was isolated from 10 kinds of ferment samples by isolation and purification, the acid qualitative and quantitative screen for producing. The strain was identified as Acetobacter cibinongensis by analysis of morphology, physiological and biochemical indicators and identification of 16S rDNA. The growth and acid-producing characteristics of strain LMY-1 was studied by fruit and vegetable culture medium with apple, pear and cucumber. Results, the optimum acid temperature for strain LMY-1 was 30 ℃； In 4 mL/100 mL ethanol content, strain LMY-1 produced the highest acid amount (22.72 g/L), higher than strain CICC7015(Acetobacter …

Effects Of Different Cleaning Sterilization Methods On Preservation Of Fresh-Cut Hang Cabbage, Lei Hao, Xie Jing, Qiao Yongxiang, Zhang Shuanshuan, Chen Yingying

Food and Machinery

Fresh Hang cabbage, through fresh cutting, was selected to study the effects of preservation with different cleaning sterilization methods (sodium hypochlorite solution with active chlorine with 0.1 g/L; electrolysis of water with: pH 2.3; ozone water: active oxygen concentration of 1.8 mg/L). The results showed that: ozone water was preferable to maintain fresh-cut Hang cabbage sensory quality, bringing the total number of colonies maintained at a low level, significantly delayed ascorbic acid content (P＜0.05), chlorophyll content reduction, and effectively extended the shelf life of Hang cabbage for 6 d.

Effect Of Processing Conditions On The Functional Properties Of Antioxidant Peptides From Pseudosciaena Crocea, Zhang Ningning, Zheng Baodong, Zhang Chong, Li Zhiyu

Food and Machinery

The functional properties of antioxidant peptides from Pseudosciaena crocea under different processing conditions were studied, including the solubility, water absorption, retention properties, fat absorption, apparent viscosity, foaming and emulsifying properties. The results showed that the isoelectric point of the antioxidant peptides was pH 4.0, and at this point, the peptides possessed the lowest solubility and emulsifying activity, while the highest emulsion stability and foaming ability and stability. Moreover, it was also found that the antioxidant peptides showed higher water and fat absorptions, fluidity and lower retention properties. Therefore, the antioxidant peptides from P. crocea could be used as potential additives …

Research Progress On Transport Vibration Damage And Effects Of Fruits On Its Postharvest Physiology Characteristics And Quality, Jiang Bao

Food and Machinery

In our country, truck transport vibration is the important reason which leads to mechanical damage and quality decreased of fruits during storage and transportation. This paper was on the basis of the analysis of the vehicle vibration in the process of truck transportation, and influencing factors, focused on the latest process in effects of transport vibration on postharvest physiology properties and quality of fruits, providing a basis and reference for the reduced transport vibration damage of fruits.

Research Status And Progress Of Fatty Acid Analysis Methods In Beans, Xie Yanhua, Xie Jing, Li Pao, Chen Miaofen, Jiang Liwen, Chen Lili

Food and Machinery

In this paper, the methods of fatty acid extraction and methyl esterification of beans (the main products are soy food) were reviewed. A variety of physiological functions of fatty acids are described. Moreover the differences of diverse detection methods of fatty acids were analyzed. We hope to provide testing methods for the change of fatty acids in bean product processing, and providing the basis for the development of potential function's in oil and fat products.

Effects Of Different Extrusion Conditions On Physicochemical Properties Of Potato Powder, Zhang Lilin, Zhang Yu, Zhang Hanyu

Food and Machinery

Several physicochemical properties of potato powder were studied, including the material moisture, extrusion temperature and screw speed's influence. The results indicated that with the increase of material moisture, the water-solubility, iodine blue value of potato powder decreasing, water-absorption, oil-absorption increasing, expansibility increased firstly and then decreased, which was the largest in moisture of 35%, while the gelatinization temperature decreased firstly then increased, and the peak, valley, final viscosity, gelatin increasing gradually. Thermal stability was the strongest in the moisture of 30%. With the increasing of extrusion temperature, the water-solubility, iodine blue value increased gradually, water-absorption, oil-absorption decreasing, and the expansibility …

Preparation And Characterization Of The Powder Microcapsules Of Cashew Nut, Chen Sida, Liang Lu, Li Yuting, Yan Xiaoyan, Liu Chengmei, Zhong Yejun, Zhong Junzhen, Xiong Yang

Food and Machinery

Cashew was used to explore its powder microcapsule, and the production methods were optimized as well as its properties and structures represented. The results showed that the emulsion stability was the best, when emulsifier ratio of single and double fat gump to sucrose ester was 1∶2. Moreover, the best emulsifier content was found to be 1.5%, and the best core wall proportion was 1∶1, with the best solid content of 25%. Basic ingredients of microcapsules consisted of proteins ( 12.94%), water (2.38%), carbohydrate （61.57%）, ash （2.45%）， and fat (20.66%). Consequently, the rate of mcrocapsule embedding rate 94.10%, with density 0.68 …

Studies On Formation Of Ages By Lysine In Maillard Reaction, Li Pu, Xia Qiuqin, Liu Guimei, Lu Yongling, Lu Lishuang

Food and Machinery

[Purpose] The different influencing factors are investigated on producing AGEs by Lysine in Maillard reaction, and the different between single amino acid (Lysine, or Argine) -reducing and mixed Lys- Arg -reducing sugar system are compared. [Method] Fluorescence spectrometry (λex / λem =370 / 440 nm) is used to detect the amount of AGEs, the factors of sort and concentration of sugar, metal ion, temperature, pH and genistein concentration in Maillard reaction and proportion of lysine and arginine in Lys/Arg-reducing sugar system was discussed. [Result] The increasing of sugar concentration, pH, or temperature, the more amounts of AGEs was produced. Fe …

The Preparation Of Igg Combining The Method Of Alpha Galactose Glucoside Enzyme With Saturated Ammonium Sulfate And Its Activity Research, Xiao Xueli, Zhang Zhen, Zhang Shenggui, Xu Rongrong, Di Rui, Lv Jindi, Liu Ya

Food and Machinery

The optimized preparation conditions of the concentration of IgG in yak blood after salting out of saturated ammonium sulfate by single factor experiments, including the selection of the hydrolysis time, pH, temperature and the amount of enzyme, as well as the Central Composite experiment, was investigated in this study. The activity of IgG was detected by ELISA and analyzed by using the Response Surface analysis (RSA). The results showed that the optimal concentration of IgG was 27.13 mg/mL when hydrolyzed for at 39 ℃ for 2 h, and enzymolyzed at pH 4.4,