Life Sciences Commons^™

An Analysis Of Functional Status In Multiple Sclerosis Patients After Progressive Non-Aerobic High-Intensity Maximal Effort Exercise (Mee), Hilda Lai, Michael Kuchera, R Dombroski, T Vardy, F Thomas, S O’Brien, P Yagnik, K Wenzel, S Stoll, Bruce Stouch, M. T. Wallin, I. Vargas,, Olufolake Odutola

Michael Kuchera

Background: Multiple Sclerosis (MS) is a disease with a wide-ranging impact on functional status. MS patient function has been assessed using Multiple Sclerosis Functional Composite Score (MSFCS). The MSFCS includes the standardized scores (Z-score) of three functional tests: the Paced Auditory Serial Addition Test (PASAT-3”) for cognitive function, 9-Hole Peg Test (9-HPT) for upper extremity function, and timed 25-foot walk (25-TW) for lower extremity function. One of the most common symptoms experienced by MS patients is severe fatigue, often brought on suddenly by aerobic exercise. Non-aerobic maximal effort exercise (MEE) is thought to increase strength without increasing fatigue. The IsoPUMP® …

Homoplastic Microinversions And The Avian Tree Of Life, Edward Braun, Rebecca Kimball, Kin-Lan Han, Naomi Iuhasz-Velez, Amber Bonilla, Jena Chojnowski, Jordan Smith, Rauri Bowie, Michael Braun, Shannon Hackett, John Harshman, Christopher Huddleston, Ben Marks, Kathleen Miglia, William Moore, Sushma Reddy, Frederick Sheldon, Christopher Witt, Tamaki Yuri

Sushma Reddy

Abstract Background Microinversions are cytologically undetectable inversions of DNA sequences that accumulate slowly in genomes. Like many other rare genomic changes (RGCs), microinversions are thought to be virtually homoplasy-free evolutionary characters, suggesting that they may be very useful for difficult phylogenetic problems such as the avian tree of life. However, few detailed surveys of these genomic rearrangements have been conducted, making it difficult to assess this hypothesis or understand the impact of microinversions upon genome evolution. Results We surveyed non-coding sequence data from a recent avian phylogenetic study and found substantially more microinversions than expected based upon prior information about …

Genetic And Acute Cpeb1 Depletion Ameliorate Fragile X Pathophysiology, Tsuyoshi Udagawa, Natalie Farny, Mira Jakovcevski, Hanoch Kaphzan, Juan Alarcon, Shobha Anilkumar, Maria Ivshina, Jessica Hurt, Kentaro Nagaoka, Vijayalaxmi Nalavadi, Lori Lorenz, Gary Bassell, Schahram Akbarian, Sumantra Chattarji, Eric Klann, Joel Richter

Natalie G. Farny

Fragile X syndrome (FXS), the most common cause of inherited mental retardation and autism, is caused by transcriptional silencing of FMR1, which encodes the translational repressor fragile X mental retardation protein (FMRP). FMRP and cytoplasmic polyadenylation element-binding protein (CPEB), an activator of translation, are present in neuronal dendrites, are predicted to bind many of the same mRNAs and may mediate a translational homeostasis that, when imbalanced, results in FXS. Consistent with this possibility, Fmr1(-/y); Cpeb1(-/-) double-knockout mice displayed amelioration of biochemical, morphological, electrophysiological and behavioral phenotypes associated with FXS. Acute depletion of CPEB1 in the hippocampus of adult Fmr1(-/y) mice …

Influence Of Env And Long Terminal Repeat Sequences On The Tissue Tropism Of Avian Leukosis Viruses, David Brown, Harriet Robinson

David C. Brown

Adsorption and penetration of retroviruses into eucaryotic cells is mediated by retroviral envelope glycoproteins interacting with host receptors. Recombinant avian leukosis viruses (ALVs) differing only in envelope determinants that interact with host receptors for subgroup A or E ALVs have been found to have unexpectedly distinctive patterns of tissue-specific replication. Recombinants of both subgroups were highly expressed in bursal lymphocytes as well as in cultured chicken embryo fibroblasts. In contrast, the subgroup A but not subgroup E host range allowed high levels of expression in skeletal muscle, while subgroup E but not subgroup A envelope glycoproteins permitted efficient replication in …

The Use Of Objective Data To Improve Interexaminer Reliability, Veronica Ferencz, Frank Casella, Jeffrey Nelson, Nicole Myers, Michael Kuchera

Michael Kuchera

BACKGROUND: In Osteopathic Manipulative Medicine (OMM) and Manual/Musculoskeletal Medicine (MMM), palpatory diagnosis is performed on a regular basis to diagnose somatic dysfunction (SD). This examination requires careful and precise touch coupled with subjective interpretation by individual examiners who may have been trained to evaluate SD through different methods. Interexaminer reliability studies aim to minimize variance by providing quantifiable scientific data to evaluate specific test protocols which can then be taught to practitioners. In a previous PCOM study, two examiners independently diagnosed innominate bone dysfunction lateralized using the ASIS compression test on a large group of subjects. A pressure monitoring system …

Nilgai Antelope In Northern Mexico As A Possible Carrier For Cattle Fever Ticks And Babesia Bovis And Babesia Bigemina., E Cardenas-Canales, J. Alfonso Ortega-Santos, Tyler A. Campbell, Zeferino Garcia-Vaquez, Antonio Cantu-Covarrubias, Julio Figueroa-Millian, Randy W. Deyoung, David G. Hewitt, Fred Bryant

Fred B. Bryant

Of 20 blood samples from nilgais from Me´ xico, five were polymerase chain reaction-positive for Babesia bigemina and one for Babesia bovis. Positive samples had the expected 170 (B. bigemina) and 291 (B. bovis) base pairs and were identical to Gen-Bank B. bigemina accession S45366 and B. bovis M38218.

Adjustment Of Value Productivity Estimates To Changes In Price And Technical Relationships , David Brown

David C. Brown

No abstract provided.

Farm To School: Strategies For Urban Health, Combatting Sprawl, And Establishing Community Food Systems, Mark Vallianatos, Robert Gottlieb, Margaret Haase

Mark Vallianatos

Farm-to-school is a new, innovative strategy with multiple planning-related objectives. The article evaluates the significance of farm-to-school in relation to improving the health and nutrition of school-age children, particularly low-income youth; strengthening the capacity of local farmers, particularly those engaged in sustainable practices; adding to the toolkit of strategies designed to contain and ultimately reduce sprawl-inducing developments by helping preserve farmland; and helping establish a community food systems approach no longer entirely dependent on the global food system that has come to dominate food growing, processing, distribution, and consumption patterns around the world.

Comparative Phyloclimatic Analysis And Evolution Of Ecological Niches In The Scimitar Babblers (Aves: Timaliidae: Pomatorhinus), Arpad Nyari, Sushma Reddy

Sushma Reddy

We present the first extensive and integrative analysis of niche evolution based on climatic variables and a dated molecular phylogeny of a heterogeneous avian group of Southeast Asian scimitar babblers of the genus Pomatorhinus. The four main clades of scimitar babblers have species that co-occur in similar areas across southern Asia but some have diverged at different timeframes, with the most recently evolved clade harboring the highest number of species. Ecological niche models and analysis of contributing variables within a phylogenetic framework indicate instances of convergent evolution of members of different clades onto similar ecological parameter space, as well …

Using Gis Mapping Of The Extent Of Nearshore Rocky Reefs To Estimate The Abundance And Reproductive Output Of Important Fishery Species, Jeremy Claisse, Daniel Pondella, Jonathan Williams, James Sadd

James Sadd

Kelp Bass (Paralabrax clathratus) and California Sheephead (Semicossyphus pulcher) are economically and ecologically valuable rocky reef fishes in southern California, making them likely indicator species for evaluating resource management actions. Multiple spatial datasets, aerial and satellite photography, underwater observations and expert judgment were used to produce a comprehensive map of nearshore natural rocky reef habitat for the Santa Monica Bay region (California, USA). It was then used to examine the relative contribution of individual reefs to a regional estimate of abundance and reproductive potential of the focal species. For the reefs surveyed for fishes (i.e. 18 …

Lower Extremity Passive Range Of Motion In Community-Ambulating Stroke Survivors, Sheila Schindler-Ivens, Davalyn Desimone, Sarah Grubich, Carolyn Kelley, Namita Sanghvi, David Brown

David C. Brown

Background: Physical therapists may prescribe stretching exercises for individuals with stroke to improve joint integrity and to reduce the risk of secondary musculoskeletal impairment. While deficits in passive range of motion (PROM) exist in stroke survivors with severe hemiparesis and spasticity, the extent to which impaired lower extremity PROM occurs in community-ambulating stroke survivors remains unclear. This study compared lower extremity PROM in able-bodied individuals and independent community-ambulatory stroke survivors with residual stroke-related neuromuscular impairments. Our hypothesis was that the stroke group would show decreased lower extremity PROM in the paretic but not the nonparetic side and that decreased PROM …

Infection Of Peripancreatic Lymph Nodes But Not Islets Precedes Kilham Rat Virus-Induced Diabetes In Bb/Wor Rats, David Brown, Raymond Welsh, Arthur Like

David C. Brown

A parvovirus serologically identified as Kilham rat virus (KRV) reproducibly induces acute type I diabetes in diabetes-resistant BB/Wor rats. The tissue tropism of KRV was investigated by in situ hybridization with a digoxigenin-labelled plasmid DNA probe containing approximately 1.6 kb of the genome of the UMass isolate of KRV. Partial sequencing of the KRV probe revealed high levels of homology to the sequence of minute virus of mice (89%) and to the sequence of H1 (99%), a parvovirus capable of infecting rats and humans. Of the 444 bases sequenced, 440 were shared by H1. KRV mRNA and DNA were readily …

Biaxial Failure Properties Of Planar Living Tissue Equivalents, Kristen Billiar, Angela Throm, Margo Frey

Kristen L. Billiar

Quantification of the mechanical properties of living tissue equivalents (LTEs) is essential for assessing their ultimate functionality as tissue substitutes, yet their delicate nature makes failure testing problematic. For this study, we evaluated the validity of using an inflation device for quantifying the biaxial tensile failure properties of extremely delicate fibroblast-populated collagen gels (CGs) and fibrin gels (FGs). Small samples were circularly clamped and then inflated until rupture. Each sample assumed an approximately spherical shape and burst at its center indicating effective clamping. After two weeks in culture, all LTEs tested were fragile, but the FGs were significantly stronger and …

Navigational Mechanisms Of Migrating Monarch Butterflies, Steven Reppert, Robert Gegear, Christine Merlin

Robert J. Gegear

Recent studies of the iconic fall migration of monarch butterflies have illuminated the mechanisms behind their southward navigation while using a time-compensated sun compass. Skylight cues, such as the sun itself and polarized light, are processed through both eyes and are probably integrated in the brain's central complex, the presumed site of the sun compass. Time compensation is provided by circadian clocks that have a distinctive molecular mechanism and that reside in the antennae. Monarchs might also use a magnetic compass because they possess two cryptochromes that have the molecular capability for light-dependent magnetoreception. Multiple genomic approaches are now being …

Food Justice And Food Retail In Los Angeles, Mark Vallianatos

Mark Vallianatos

No abstract provided.

Acute Effects Of Whole-Body Vibration On Lower Extremity Muscle Performance In Persons With Multiple Sclerosis, Kurt Jackson, Harold Merriman, Paul Vanderburgh, C. Brahler

C. Jayne Brahler

Background and Purpose: Whole-body vibration (WBV) is a relatively new form of exercise training that may influence muscle performance. This study investigated the acute effects of high (26 Hz) and low (2 Hz) frequency WBV on isometric muscle torque of the quadriceps and hamstrings in persons with multiple sclerosis (MS).

Participants and Method: Fifteen individuals (mean age = 54.6 years, SD = 9.6) with MS and Expanded Disability Status Scale (EDSS) scores ranging from 0-6.5 (mean = 4.2, SD = 2.3) participated in this randomized cross-over study. Following baseline measures of isometric quadricep and hamstring torque, subjects were exposed to …

Inter-Examiner Reliability Of An Anterior Superior Iliac Spine Compression Test Used To Lateralize Pelvic Somatic Dysfunction To The Right Side Or Not, Frank Casella, Michael Kuchera, Nicole Myers, Jeffrey Nelson, Veronica Ferencz

Michael Kuchera

BACKGROUND: Osteopathic physicians use a number of palpatory structural examinations to diagnose pelvic somatic dysfunction (SD). They may elect to use the Anterior Superior Iliac Spine (ASIS) Compression Test to lateralize the dysfunctional side. Accurate, reliable tests are crucial to neuromusculoskeletal diagnosis and this study employs the kappa (κ) analysis protocol recommended for assessing interexaminer reliability of manual medicine tests (published by the Fédération Internationale de Médecine Manuelle [FIMM]). κ-values ≥0.40 (moderate agreement) are considered to be acceptable for use in the clinical setting.

Healthy School Food Policies: A Checklist, Mark Vallianatos

Mark Vallianatos

No abstract provided.

Loading Rate In Self-Initiated Vertical Jump Landings: Developmental And Gender Comparisons, Pamela Russell, Erik Swartz, Ron Croce, Laura Decoster

Pamela J. Russell

The study compared gender and developmental differences in vertical loading rate upon a two-footed landing from a self-initiated VJ. Fifty-seven subjects grouped by age (pre-pubescent (8-11 yrs); post-pubescent (19-29 yrs)) and gender consented to participate. Subjects jumped for a ball set at 50% of their maximum VJ height, and landed on two feet, facing forward, with only their dominant foot on the force plate. Motion analysis (3-D) and ground reaction force (GRF) data were collected. Statistical analyses indicated significant developmental differences in vertical loading rate normalized to kinetic energy, but no gender differences. Children may have higher loading rates because …

But What Is It That You Actually Do? (What It's Really Like Working In The Lab), Michael Reagan

Michael S. Reagan

No abstract provided.

The Association Between Critical Thinking And Scholastic Aptitude On First-Time Pass Rate Of The National Physical Therapy Examination, Daniel Suckow, C. Brahler, Betsy Donahoe-Fillmore, Mary Fisher, Philip Anloague

C. Jayne Brahler

Objectives: 1) To investigate the relationships among critical thinking (CT) abilities, overall academic performance in the Doctorate of Physical Therapy (DPT) program as measured by cumulative grade point average (GPA), and National Physical Therapy Examination (NPTE) licensure scores, and 2) To determine if NPTE scores were significantly different between groups of students who were classified as having low, moderate or high CT abilities. Background: It is well-established that physical therapy practice requires good clinical reasoning skills. Passage of the NPTE is required for licensure. Research to date has been mixed as to whether CT abilities or GPA can predict success …

Substrate Rigidity Regulates The Formation And Maintenance Of Tissues, Wei-Hui Guo, Margo Frey, Nancy Burnham, Yu-Li Wang

Nancy A. Burnham

The ability of cells to form tissues represents one of the most fundamental issues in biology. However, it is unclear what triggers cells to adhere to one another in tissues and to migrate once a piece of tissue is planted on culture surfaces. Using substrates of identical chemical composition but different flexibility, we show that this process is controlled by substrate rigidity: on stiff substrates, cells migrate away from one another and spread on surfaces, whereas on soft substrates they merge to form tissue-like structures. Similar behavior was observed not only with fibroblastic and epithelial cell lines but also explants …

Comparing Inter-Examiner Reliability Levels When Diagnosing Male & Female Innominate Dysfunctions Using A Hemi-Pelvise Compression Lateralization Test And Pelvic Landmark Levels., Nicole Myers, Michael Kuchera, Veronica Ferencz, Jeffrey Nelson, Frank Casella

Michael Kuchera

BACKGROUND: When diagnosing innominate somatic dysfunctions it may be relevant to recognize that structural, functional, and hormonal differences exist between male and female pelvises. The female pelvis is less massive, ilia are less sloped, and female hormones influence ligamentous tension. Despite these differences, few studies have analyzed gender effects on inter-examiner reliability when using palpatory diagnosis to diagnose innominate dysfunctions. In this study, we hypothesized that interexaminer reliability would be higher in male subjects than in female subjects due cyclic variability of hormonal influence of ligamentous tension in the female pelvis. The kappa (κ) statistic was selected to evaluate inter-examiner …

Discordant Timing Between Antennae Disrupts Sun Compass Orientation In Migratory Monarch Butterflies, Patrick Guerra, Christine Merlin, Robert Gegear, Steven Reppert

Robert J. Gegear

To navigate during their long-distance migration, monarch butterflies (Danaus plexippus) use a time-compensated sun compass. The sun compass timing elements reside in light-entrained circadian clocks in the antennae. Here we show that either antenna is sufficient for proper time compensation. However, migrants with either antenna painted black (to block light entrainment) and the other painted clear (to permit light entrainment) display disoriented group flight. Remarkably, when the black-painted antenna is removed, re-flown migrants with a single, clear-painted antenna exhibit proper orientation behaviour. Molecular correlates of clock function reveal that period and timeless expression is highly rhythmic in brains and clear-painted …

Nesting Piping Plover And Least Tern On The Kansas River, William Busby, Daniel Mulhern, P. Gregory Kramos, David Rintoul, William Tuttle

Daniel Mulhern

A portion of the Kansas River in northeastern Kansas was surveyed by boat and air in 1996 and 1997 for nesting colonies of piping plover (Charadrius melodus) and least tern (Sterna antillarum). Both species were found breeding on sandbars at a total of five sites along a 30-km reach of the Kansas River. In 1996, at least two breeding pair of piping plovers and seven breeding pair of least terns were documented. In 1997, at least one pair of piping plovers and five pairs of least terns bred. These are the first known breeding records for the piping plover in …

On The Edge: A Genetic Assessment Of Aplodontia Rufa From The Edge Of Their Distribution, Antoinette Piaggio, Jennifer Jeffers

Jennifer M. Jeffers

Aplodontia rufa (mountain beaver) is considered the sole remaining extant genus and species of an ancient lineage that once broadly inhabited the Great Basin and is now restricted to the Pacific Northwest and portions of California and Nevada. Aplodontia rufa californica in Nevada is distributed patchily at the edge of mountain beaver distribution. Due to concern over the status of these populations this subspecies is listed in Nevada as sensitive. The Nevada Department of Wildlife is concerned about the status of Aplodontia rufa californica populations scattered across areas of central western Nevada and has worked to gain an understanding of …

A New Species Of Boubou (Malaconotidae: Laniarius) From The Albertine Rift, Gary Voelker, Robert Outlaw, Sushma Reddy, Michael Tobler, John Bates, Shannon Hackett, Charles Kahindo, Ben Marks, Julian Kerbis Peterhans, Thomas Gnoske

Sushma Reddy

We describe Laniarius willardi, a new species of boubou shrike (Malaconotidae) from the Albertine Rift of Africa. The most conspicuous, distinguishing morphological feature of the species is a gray to blue-gray iris. This and external morphometric data indicate that L. willardi is diagnosable from other black or sooty boubous. Further, L. willardi is genetically diagnosable, and its closest relative is the Mountain Sooty Boubou (L. poensis camerunensis) from Cameroon. The Crimson-breasted Bush-shrike (L. atrococcineus) and the Lowland Sooty Boubou (L. leucorhynchus) are together the sister clade to L. willardi—L.p. camerunensis. Laniarius willardi and the geographically codistributed L. p. holomelas differ …

Elevated Co2 Induced Changes In The Chemistry Of Quaking Aspen (Populus Tremuloides Michaux) Leaf Litter: Subsequent Mass Loss And Microbial Response In A Stream Ecosystem, Steven Rier, Nancy Tuchman, Robert Wetzel, James Teeri

Nancy Tuchman

No abstract provided.

Unveiling The Impact Of Human Influence On Species Distributions In Vietnam: A Case Study Using Babblers (Aves: Timaliidae), Laurel Yohe, Johnathan Flanders, Hoang Minh Duc, Long Vu, Sushma Reddy

Sushma Reddy

As developing countries give priority to economic growth, the effects of development threaten natural habitats and species distributions. Over the course of two decades, Vietnam has rapidly developed, especially in the expansion of agricultural production. However, no study has quantitatively measured the effects of recent human impact on the effects of past species distributions in Vietnam. We use locality data collected from multiple natural history collections, including several in Vietnam, to infer past species distributions. We assess habitat availability of five common babbler species (Aves: Timaliidae) using distribution models with data prior to rapid development that followed political reform. Overlaying …

Antennal Circadian Clocks Coordinate Sun Compass Orientation In Migratory Monarch Butterflies, Christine Merlin, Robert Gegear, Steven Reppert

Robert J. Gegear

During their fall migration, Eastern North American monarch butterflies (Danaus plexippus) use a time-compensated Sun compass to aid navigation to their overwintering grounds in central Mexico. It has been assumed that the circadian clock that provides time compensation resides in the brain, although this assumption has never been examined directly. Here, we show that the antennae are necessary for proper time-compensated Sun compass orientation in migratory monarch butterflies, that antennal clocks exist in monarchs, and that they likely provide the primary timing mechanism for Sun compass orientation. These unexpected findings pose a novel function for the antennae and open a …