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Effects Of Oocyte Source On The Developmental Competence Of In Vitro Matured Goat Oocytes Fertilized By The Intracytoplasmic Sperm Injection Technique, Abu Nasar Md. Aminoor Rahman, Ramli Bin Abdullah, Wan Embong Wan-Khadijah
Effects Of Oocyte Source On The Developmental Competence Of In Vitro Matured Goat Oocytes Fertilized By The Intracytoplasmic Sperm Injection Technique, Abu Nasar Md. Aminoor Rahman, Ramli Bin Abdullah, Wan Embong Wan-Khadijah
Turkish Journal of Veterinary & Animal Sciences
This study evaluated the effects of oocyte source on in vitro maturation (IVM) and embryo developmental competence in goats by the intracytoplasmic sperm injection (ICSI) technique. Oocytes were obtained either by laparoscopic ovum pick-up (LOPU) or by slicing of abattoir ovaries. The donor does used for LOPU were superstimulated and their estrus was synchronized. Recovered oocytes from both sources were cultured in microdrops of IVM medium in the presence of CO2 (5%) at 38.5 °C in a CO2 incubator for 27 h. The ICSI was performed with immobilized sperm on the lid of a culture dish (35 mm) under an …
Isolation And Biotyping Of Brucella Melitensis From Aborted Sheep And Goat Fetuses, Esra Büyükcangaz, Ayşi̇n Şen, Serpi̇l Kahya
Isolation And Biotyping Of Brucella Melitensis From Aborted Sheep And Goat Fetuses, Esra Büyükcangaz, Ayşi̇n Şen, Serpi̇l Kahya
Turkish Journal of Veterinary & Animal Sciences
The possible role of Brucella spp. in 65 abortion cases, 55 from sheep and 10 from goats, occurring in the birth seasons of 2004 and 2005 in northwestern Turkey was investigated. Colony morphology, agglutination by acriflavin, H2S production, CO2 requirement, dye sensitivity in thionin, basic fuchsin, growth characteristics in streptomycin, lysis with Tbilisi phage, and agglutination with monospecific A- and M antisera were examined for identification and biotyping. The isolates from 21 of 55 sheep and 1 of 10 goat abortion cases were identified as Brucella melitensis. Biotyping revealed that 14, 6, and 1 of 22 Brucella spp. were B. …