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Isolation Of Aerobic Bacterial Agents From The Lungs Of Sheep And Goats With Pneumonia And Detection Of Pasteurella Multocida And Mannheimia Haemolytica By Polymerase Chain Reaction, Gökben Özbey, Adi̇le Muz
Isolation Of Aerobic Bacterial Agents From The Lungs Of Sheep And Goats With Pneumonia And Detection Of Pasteurella Multocida And Mannheimia Haemolytica By Polymerase Chain Reaction, Gökben Özbey, Adi̇le Muz
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to isolate Pasteurella spp and other aerobic bacterial agents from the lungs of sheep and goats with pneumonia and to identify Pasteurella multocida and Mannheimia haemolytica by both culture methods and polymerase chain reaction (PCR). In addition, mouse pathogenecity tests were carried out on suspected P. multocida isolates. In the examination of lung samples collected from sheep, 15 (4.3%) P. multocida and 8 (2.3%) M. haemolytica strains were isolated and identified. The numbers of species identified in the goat samples were 1 (0.7%) for P. multocida and 6 (4%) for M. haemolytica. The differences …
Isolation Of Bacterial Agents From The Lungs Of Cattle With Pneumonia And Detection Of Pasteurella Spp. By Polymerase Chain Reaction, Ayşe Kiliç, Adi̇le Muz
Isolation Of Bacterial Agents From The Lungs Of Cattle With Pneumonia And Detection Of Pasteurella Spp. By Polymerase Chain Reaction, Ayşe Kiliç, Adi̇le Muz
Turkish Journal of Veterinary & Animal Sciences
Lungs from 8222 cattle slaughtered at an abattoir in Elazığ were examined macroscopically, and pneumonia was detected in 500 (6.1%) lungs. These samples were inoculated onto blood agar supplemented with 7% sheep blood for isolation of bacterial agents. A polymerase chain reaction (PCR) based upon the use of species-specific primers was carried out on DNA samples extracted from suspected Pasteurella spp. isolates. In addition, a mouse inoculation test was carried out on suspected Pasteurella multocida isolates. A total of 311 (62.2%) bacterial agents were isolated from the lung samples and were identified as 7.2% Staphylococcus aureus, 6% Pasteurella multocida, 6% …