Life Sciences Commons^™

Investigation Of The Nontypical Pasteurella Multocida Strains Obtained From Multiple Sources, Regions, And Times: An Unexpected Increase Was Detected, Asli Sakmanoğlu, Ali̇ Uslu, Zafer Sayin, Yasemi̇n Karyeyen, Gökçenur Sani̇oğlu Gölen, Ayşegül İlban, Beatri̇z Padron-Perez, Mustafa Agah Teki̇ndal, Osman Ergani̇ş

Turkish Journal of Veterinary & Animal Sciences

In this study, it was aimed to isolate Pasteurella multocida from 1250 lung samples (calf, sheep, and goat), with respiratory system infection, such as nasal discharge, cough, fever, and to investigate the phenotypic and genotypic properties of this bacteria. As a result, a total of 92 P. multocida were obtained, of which 66 (71.73%) were from calf, 19 (20.65%) from sheep, and 7 (7.6%) from goat. The A:3A type was the most common type with 34 isolates (36.95%), including 30 from calves (45.45%), and 4 from sheep (21.05%). An alternate mPCR protocol was developed to determine capB and capE genes …

A Phylogenetic Analysis Of Mycoplasma Strains Circulating In Sheep Pneumonia In Thekars Region Of Turkey, Olcay Öztürkler, Sali̇h Otlu

Turkish Journal of Veterinary & Animal Sciences

This study aimed to investigate Mycoplasma species by cultural and molecular methods in 250 pneumonics and 30 healthy appearance sheep lungs brought from slaughterhouse and butcheries in the Kars region of Turkey. The phylogenetic positioning of the bacterial strains to be obtained in the present study was another remarkable outcome of the study. Mycoplasma isolation was achieved solely in 26 (10.4%) of 250 sheep lungs with pneumonia. Mycoplasma ovipneumoniae was found predominant species in 12 (46.15%) of samples and that was followed by Mycoplasma arginini in 4 (15.38%) of strains. Moreover, two samples were found to be co-infected with the …

Nlrp6 In Gram-Positive Pneumonia And Sepsis, Laxman Ghimire

LSU Doctoral Dissertations

Acute lower respiratory infections (pneumonia) and pneumonia-derived sepsis are among the leading causes of death in the world causing 7.8 million deaths annually. In this regard, Methicillin-resistant Staphylococcus aureus (MRSA) is endemic in the US and implicated for causing high mortality-associated necrotizing pneumonia and aggravating viral pneumonia with superinfection. Additionally, sepsis is the 7^th leading cause of death among newborns in the US and is responsible for more than 750,000 hospitalization cases every year. Although there is a plethora of research in both pneumonia and sepsis, the detailed pathophysiology still remains elusive. Understanding the host defense mechanism will help …

Antimicrobial Resistance And Phenotypic And Molecular Detection Of Extended-Spectrum Ss-Lactamases Among Extraintestinal Escherichia Coli Isolated From Pneumonic And Septicemic Sheep And Goats In Rajasthan, India, Fateh Singh, Ganesh Gangaram Sonawane, Jyoti Kumar, Shivendra Kumar Dixit, Rajendra Kumar Meena, Bhupendra Nath Tripathi

Turkish Journal of Veterinary & Animal Sciences

The present study was planned to isolate Escherichia coli from pneumonic and septicemic sheep and goats and to determine antimicrobial resistance (AMR) patterns of the isolates and evaluate them for the presence of extended-spectrum ß-lactamase (ESBL) using the phenotypic and molecular methods. Pneumonic lung tissues and heart blood samples were collected by performing necropsy on sheep (n = 96) and goats (n = 08). The samples were processed and used for bacterial isolation. E. coli isolates (n = 58) including 53 from 34 sheep and 5 from 3 goats were recovered and identified by the cultural and biochemical characteristics and …

Comparative Detection Of Bovine Herpesvirus-1 Using Antigen Elisa, Immunohistochemistry And Immunofluorescence Methods In Cattle With Pneumonia, Seli̇m Çomakli, Yavuz Seli̇m Sağlam, Mehmet Özkan Ti̇murkan

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to detect the prevalence and presence of bovine herpesvirus-1 (BoHV-1) using immunohistochemistry (IHC), immunofluorescence (IF) and antigen enzyme-linked immunosorbent assay (ELISA) in the lung samples of cattle (n = 1023). In addition, three methods were compared using receiver operating characteristic (ROC) and chi-square test ($χ^{2}$ test), and their usability were evaluated in laboratory conditions. Macroscopically, pneumonia was seen in 120 of the lung tissue samples (11.73%). Based on the microscopic examinations, the pneumonia types were classified as catarrhal-suppurative bronchopneumonia (6.7%), fibrinous bronchopneumonia (5%), interstitial pneumonia (84.1%), and granulomatous pneumonia (4.2%). The IHC, IF and antigen ELISA …

Immunohistochemical Expression Of Secretory Proteins In Pneumonic Rat Lungs, Enver Beytut

Turkish Journal of Veterinary & Animal Sciences

The present study evaluated the expression of secretory proteins involving surfactant proteins (SP-A, SP-B, proSP-C), Clara cell secretory protein (CCSP), and thyroid transcription factor-I (TTF-I), together with lambda light chain IgG (λ-IgG), proliferating cell nuclear antigen (PCNA), and lymphocytic phenotypes (T and B cells) in the pneumonic rat lungs. The most prominent gross lesion was severe pulmonary abscession. The lungs showed severe parenchymal destruction and necrosis encapsulated by fibrous tissue. Immunostaining for SPs displayed evident hyperplasia of type II pneumocytes. Immunoreaction to SP-A and SP-B occurred strongly on the luminal surface of type II cells, and to proSP-C in the …

Mycoplasma Arginini: High Frequency Involvement In Goat Pneumonia, Rekha Valsala, Rajneesh Rana, Arun Thachappully Remesh, Vijendra Pal Singh

Turkish Journal of Veterinary & Animal Sciences

Mycoplasmas are an important class of bacteria associated with caprine pneumonia. Some of the main species involved are M. mycoides subsp. capri, M. capricolum subsp. capricolum, and M. capricolum subsp. capripneumoniae. In addition, there are frequent reports of M. arginini involvement. In the present study an attempt was made to see the association of various mollicutes in caprine pneumonia. In total 244 pneumonic goat lung samples collected over a 10-year period (2003-2012) were screened for the isolation and identification of associated mollicutes. The identification was done on the basis of biochemical tests, growth inhibition test, PCR, and sequencing. The most …

The Pathologic And Bacteriologic Comparison Of Pneumonia In Lambs, Ertan Oruç

Turkish Journal of Veterinary & Animal Sciences

The correlations between the bacteriologic agent of lamb pneumonia and its pathologic characters were investigated in this study. Pneumonia was detected in 262 (35.41%) of 740 diseased or dead lambs. Pathogenic bacteria were isolated in 114 cases (14.96% of all lamb deaths and 43.51% of all pneumonias). Lung lesions were histopathologically described as acute-catarrhal (17.56%), catarrhal-purulent (14.50%), purulent-necrotic (9.54%), fibrinous (26.72%), fibrinous-necrotic (5.73%), interstitial (18.32%), or verminous pneumonia (7.63%). In bacteriological studies, Mannheimia haemolytica (56.14%), E. coli (24.56%), and Pasteurella multocida (10.52%) were detected as important bacterial agents that caused different pneumonic lesions in lambs.

Isolation Of Aerobic Bacterial Agents From The Lungs Of Sheep And Goats With Pneumonia And Detection Of Pasteurella Multocida And Mannheimia Haemolytica By Polymerase Chain Reaction, Gökben Özbey, Adi̇le Muz

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to isolate Pasteurella spp and other aerobic bacterial agents from the lungs of sheep and goats with pneumonia and to identify Pasteurella multocida and Mannheimia haemolytica by both culture methods and polymerase chain reaction (PCR). In addition, mouse pathogenecity tests were carried out on suspected P. multocida isolates. In the examination of lung samples collected from sheep, 15 (4.3%) P. multocida and 8 (2.3%) M. haemolytica strains were isolated and identified. The numbers of species identified in the goat samples were 1 (0.7%) for P. multocida and 6 (4%) for M. haemolytica. The differences …

Isolation Of Bacterial Agents From The Lungs Of Cattle With Pneumonia And Detection Of Pasteurella Spp. By Polymerase Chain Reaction, Ayşe Kiliç, Adi̇le Muz

Turkish Journal of Veterinary & Animal Sciences

Lungs from 8222 cattle slaughtered at an abattoir in Elazığ were examined macroscopically, and pneumonia was detected in 500 (6.1%) lungs. These samples were inoculated onto blood agar supplemented with 7% sheep blood for isolation of bacterial agents. A polymerase chain reaction (PCR) based upon the use of species-specific primers was carried out on DNA samples extracted from suspected Pasteurella spp. isolates. In addition, a mouse inoculation test was carried out on suspected Pasteurella multocida isolates. A total of 311 (62.2%) bacterial agents were isolated from the lung samples and were identified as 7.2% Staphylococcus aureus, 6% Pasteurella multocida, 6% …

In Situ Hybridization For The Detection And Localization Of Swine Chlamydia Trachomatis, C. Chae, D.-S. Cheon, D. Kwon, O. Kim, B. Kim, J. Suh, D. G. Rogers, K. D. E. Everett, A. A. Anderson

School of Veterinary and Biomedical Sciences: Faculty Publications

Gnotobiotic piglets were inoculated intralaryngeally with swine Chlamydia trachomatis strain R33 or orally with swine C. trachmatis strain R27. Archived formalin-fixed, paraffin-embedded tissues from piglets euthanatized 4–7 days postinoculation were examined by in situ hybridization for C. trachomatis nucleic acid using a nonradioactive digoxigenin-labeled DNA probes that targeted specific ribosomal RNA or omp1 mRNA molecules of the swine C. trachomatis strains. Positive hybridization signals were detected in bronchial epithelial cells, bronchiolar epithelial cells, pneumocytes, alveolar and interstitial macrophages, and jejunal and ileal enterocytes. Chlamydia-infected cells had a strong signal that was confined to the intracytoplasmic inclusions. Positive hybridization signals were …

Virus Pneumonia Of Pigs, J Craig, N. Macintyre

Journal of the Department of Agriculture, Western Australia, Series 4

THIS disease is widespread in Western Australia and is undoubtedly a most serious source of economic loss to the pig industry.

The effects of virus pneumonia are such that in badly managed herds, widespread pneumonic outbreaks and deaths are common.

By contrast, in well managed herds, there is the more insidious problem of low or nil death rates, accompanied however by an appreciable fall in animal production.

Feeding And Care Of Calves, R. R. Thalman

Nebraska Agricultural Experiment Station: Historical Circulars

It is not infrequent that considerable difficulty is encountered in raising calves with limited amounts of milk or none at all. Requests for information on calf gruels, mixed grain feeds, and commercial supplements are frequent enough to make a short circular upon this subject seem desirable. Furthermore, as the more diversified agricultural program gets under way these requests are increasmg.

Swine Sanitation, L. Van Es

Nebraska Agricultural Experiment Station: Historical Circulars

From the very beginning of Nebraska's agricultural development its farmers have recognized that the production of swine must of necessity accompany the growing of corn. The latter, one of the state's most important staples, cannot be marketed in a more economical manner than after having been transformed into pork, bacon, and lard. As a result the state has for many years maintained a rather dense swine population mainly divided into large herds kept on relatively small areas of land. This density of population, as well as certain practices in management and selective breeding, has brought about conditions favorable for the …