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A Proposed Mechanism Of Action For In Vitro Excystation Of Two Species Of Coccidia, William Ronald Jolley
A Proposed Mechanism Of Action For In Vitro Excystation Of Two Species Of Coccidia, William Ronald Jolley
Theses and Dissertations
Excystation of coccidian oocysts is accomplished in vitro by altering wall permeability with CO2 and a reducing agent, then activating the enclosed sporozoites with a solution of trypsin and bile. Elucidation of the mechanism of action of the CO2-reducing agent treatment was the basic intent of this study. Oocysts of Eimeria stiedae (rabbit) and E. tenella (chicken) were tested for the presence of an excystation-associated enzyme by incubating sporulated oocysts in fluids extracted from variously treated oocysts, and by carbon-14 labelling; the effect of CO2 -reducing agent treatment on oocyst walls was investigated by titration with acid or base for …