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Full-Text Articles in Life Sciences
32p-Postlabeling Analysis Of Aromatic Dna Adducts In Hemopoietic Tissues And Blood Of The Mummichog Fundulus Heteroclitus, Wendy L. Rose
32p-Postlabeling Analysis Of Aromatic Dna Adducts In Hemopoietic Tissues And Blood Of The Mummichog Fundulus Heteroclitus, Wendy L. Rose
Dissertations, Theses, and Masters Projects
No abstract provided.
Functional And Molecular Analysis Of Antibody Affinity Maturation In Rainbow Trout (Oncorhynchus Mykiss), Haili Zhang
Functional And Molecular Analysis Of Antibody Affinity Maturation In Rainbow Trout (Oncorhynchus Mykiss), Haili Zhang
Dissertations, Theses, and Masters Projects
No abstract provided.
Indo-Pacific Population Structure Of The Black Marlin, Makaira Indica, Inferred From Molecular Markers, Brett Falterman
Indo-Pacific Population Structure Of The Black Marlin, Makaira Indica, Inferred From Molecular Markers, Brett Falterman
Dissertations, Theses, and Masters Projects
No abstract provided.
Environmental Stress In Hard Coral: Evaluating Lipid As An Indicator Of Sublethal Stress On Short Time Scales, David Harold Niebuhr
Environmental Stress In Hard Coral: Evaluating Lipid As An Indicator Of Sublethal Stress On Short Time Scales, David Harold Niebuhr
Dissertations, Theses, and Masters Projects
Lipid quality was evaluated in Montastrea spp. under sediment- and heat-stressed conditions to evaluate lipid ratio as an indicator of sub-lethal stress on short time scales. The ratio of storage lipid (wax ester + triacylglyceride) to structural lipid (sterol esters + phospholipid) decreased significantly (0.25 to 0.14, p < 0.01) after experimental sedimentation. FAME analysis of colonies exposed to experimental sedimentation showed a reduction of the algal, 18:3(n-6) and 18:4(n-3), polyunsaturated fatty acid (PUFA) in the polar lipid fraction. This loss of PUFA suggests a loss of algal membrane in sediment-stressed colonies. Lipid quality was similarly measured in M. faveolata over a 10-day period. Mean (n = 20) ratio of storage to structural lipid in M. faveolata dropped from 2.43 to a level of 0.98 immediately following a natural sedimentation event before recovering to levels of 1.4 and 2.9 on post-storm days 2 and 4, respectively. Colonies of M. annularis subject to heat-stress (35??C) exhibited no significant change in storage lipid ratio, while levels of Free Fatty Acids increased significantly from 0.012 (n = 22) to 0.156 mg lipid/g dry tissue (n = 22)(p < 0.05). FAME analysis of tissue lipids extracted from the heat-stressed colonies showed changes in the polar fraction, with significant decreases in the 18:3(n-6), 18:3(n-3), 18:4(n-3), 20:4(n-6) and 20:5(n-3) (p < 0.05) PUFA and subsequent significant increases in the saturated fatty acids, 16:0 and 18:0 (p < 0.05). These changes in lipid quantity and quality indicate possible oxidation and preferential digestion of zooxanthellar membranes. Stress experiments were repeated in M. annularis using VacutainerRTM blood collection tubes to collect micro-tissue samples without destroying skeleton of the sample colonies. A significant decrease in storage: structural lipid ratio after sedimentation was also detected using the micro-tissue technique. This study indicates that the relative abundance of lipid subclass components can indicate sub-lethal environmental stress, on short time scales, in M. annularis and M. faveolata. Furthermore, micro-tissue collection techniques permit repeated monitoring coral colonies to assess the manifestation of stress from first detection of impact at the cellular level to changes in community to changes in community structure detectable over longer time scales.
Molecular Phylogeny Of Lake Baikal Amphipods, Kenneth S. Macdonald
Molecular Phylogeny Of Lake Baikal Amphipods, Kenneth S. Macdonald
Dissertations, Theses, and Masters Projects
No abstract provided.
The Phylogeny Of Coleoid Cephalopods Inferred From Molecular Evolutionary Analyses Of The Cytochrome C Oxidase I, Muscle Actin, And Cytoplasmic Actin Genes, David Bruno Carlini
The Phylogeny Of Coleoid Cephalopods Inferred From Molecular Evolutionary Analyses Of The Cytochrome C Oxidase I, Muscle Actin, And Cytoplasmic Actin Genes, David Bruno Carlini
Dissertations, Theses, and Masters Projects
Although the fossil record of early cephalopods is rich and demonstrates the dominance of the group in Paleozoic times, the mainly soft-bodied coleoids (Cephalopoda: Coleoidea) are poorly represented. Therefore, little is known of the evolutionary history of coleoids through paleontology and current classifications of the subclass are based primarily on the morphology of extant representatives. A molecular phylogenetic analysis of the Coleoidea was therefore warranted. Phylogenetic relationships within the Coleoidea were constructed using molecular sequence data from one mitochondrial and two nuclear genes: cytochrome c oxidase I (COI) and two unlinked actin genes (Actin I and Actin II, respectively). A …
Comparative Evolution Of Molecular Markers: An Analysis Of Genetic Variation Within The Blue Marlin (Makaira Nigricans), Vincent Patrick Buonaccorsi
Comparative Evolution Of Molecular Markers: An Analysis Of Genetic Variation Within The Blue Marlin (Makaira Nigricans), Vincent Patrick Buonaccorsi
Dissertations, Theses, and Masters Projects
Blue marlin diversity was assessed at mtDNA, scnDNA, microsatellite DNA, and allozyme molecular markers. Hierarchical analysis of molecular variance (AMOVA) revealed that most genetic variation was maintained within populations, with a non-significant fraction attributable to variation among temporal replicates and between locations within oceans. In contrast, inter-ocean divergence was highly significant for a majority of loci within each marker class. Previous studies of mitochondrial DNA (mtDNA; n = 104) genetic variation within the blue marlin revealed two distinct clades of haplotypes, one of which was present only in the Atlantic (the 'Atlantic clade'), at a frequency of 40% &(F\sb{lcub}st{rcub}& = …
Phylogeny Of The Haplosporidia (Eukaryota: Alveolata) Based On Small Subunit Ribosomal Rna Gene Sequence, Brenda Sandy Flores
Phylogeny Of The Haplosporidia (Eukaryota: Alveolata) Based On Small Subunit Ribosomal Rna Gene Sequence, Brenda Sandy Flores
Dissertations, Theses, and Masters Projects
No abstract provided.
Expression Of The Xenobiotic Transporter P-Glycoprotein In The Mummichog (Fundulus Heteroclitus), Peter S. Cooper
Expression Of The Xenobiotic Transporter P-Glycoprotein In The Mummichog (Fundulus Heteroclitus), Peter S. Cooper
Dissertations, Theses, and Masters Projects
The mammalian mdr1 P-glycoprotein (Pgp) has been implicated in xenobiotic resistance of drug-resistant cell lines and tumors, and may function in excretion or exclusion of toxic xenobiotics at several sites within the body. Pgp gene family members are expressed in excretory epithelia of several aquatic taxa and may contribute to the survival of pollution-tolerant aquatic animals in contaminated environments. For this reason the expression of Pgp family members was investigated in the livers and liver tumors of creosote-resistant mummichog (Fundulus heteroclitus) inhabiting a creosote-contaminated site (Atlantic Wood) in the Elizabeth River, Virginia. Expression of members of the Pgp gene family …
Novel Suicide Elements For Gene Disruption In Escherichia Coli, Brian Christopher Nicholson
Novel Suicide Elements For Gene Disruption In Escherichia Coli, Brian Christopher Nicholson
Dissertations, Theses, and Masters Projects
No abstract provided.
Mitochondrial Dna Variation In Natural And Cultured Populations Of The Bay Scallop, Argopecten Irradians (Lamarck) And The Calico Scallop, Argopecten Gibbus (Dall), Sandra Grace Blake
Mitochondrial Dna Variation In Natural And Cultured Populations Of The Bay Scallop, Argopecten Irradians (Lamarck) And The Calico Scallop, Argopecten Gibbus (Dall), Sandra Grace Blake
Dissertations, Theses, and Masters Projects
No abstract provided.
Global Phylogeography Of Yellowfin Tuna, Thunnus Albacares, And Mackerels Of The Genus Scomber, Daniel R. Scoles
Global Phylogeography Of Yellowfin Tuna, Thunnus Albacares, And Mackerels Of The Genus Scomber, Daniel R. Scoles
Dissertations, Theses, and Masters Projects
Intraspecific genetic relationships within yellowfin tuna, Thunnus albacares, and three mackerels of the genus Scomber were studied by restriction fragment length polymorphism (RFLP) analysis of mitochondrial DNA (mtDNA). The comparison of these scombrids, with different larval distributions, adult distributions, and vagilities, served to investigate the hypothesis that population structure in marine fishes results from geographic and physical oceanographic processes that limit dispersal of early life history stages. Samples of 20 yellowfin tuna were examined from each of five Pacific locations and one Atlantic location. MtDNA analysis with 12 informative restriction endonucleases demonstrated considerable genetic variation, as evidenced by overall nucleon …
Xenopus Laevis Ribosomal Protein S1: Evidence For Regulation At The Transcriptional Level, Cleve Ray-Dean Sinor
Xenopus Laevis Ribosomal Protein S1: Evidence For Regulation At The Transcriptional Level, Cleve Ray-Dean Sinor
Dissertations, Theses, and Masters Projects
No abstract provided.
Cytophotometric Estimation Of Nuclear Dna Content Variation In Ten Species Of Geniculate Coralline Algae (Corallinaceae, Rhodophyta), Jeffrey Craig Bailey
Cytophotometric Estimation Of Nuclear Dna Content Variation In Ten Species Of Geniculate Coralline Algae (Corallinaceae, Rhodophyta), Jeffrey Craig Bailey
Dissertations, Theses, and Masters Projects
No abstract provided.
Mitochondrial Dna Variation In Striped Bass, Morone Saxatilis, From The Rappahannock River, Virginia, Carol Furman
Mitochondrial Dna Variation In Striped Bass, Morone Saxatilis, From The Rappahannock River, Virginia, Carol Furman
Dissertations, Theses, and Masters Projects
No abstract provided.
Lac Operon Expression In Steady State Cells Of Escherichia Coli, Sungae Cho
Lac Operon Expression In Steady State Cells Of Escherichia Coli, Sungae Cho
Dissertations, Theses, and Masters Projects
No abstract provided.
The Pattern Of Rna Synthesis In Dormant And Germinating Statoblasts Of Pectinatella Magnifica, Virginia Kent Proud
The Pattern Of Rna Synthesis In Dormant And Germinating Statoblasts Of Pectinatella Magnifica, Virginia Kent Proud
Dissertations, Theses, and Masters Projects
No abstract provided.
A Comparison Of Ribosomal Ribonucleic Acid Using Disc Electrophoresis, Carolyn Gene Mohler
A Comparison Of Ribosomal Ribonucleic Acid Using Disc Electrophoresis, Carolyn Gene Mohler
Dissertations, Theses, and Masters Projects
No abstract provided.