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How Acetylation Regulates Metabolic Enzyme Function During Environmental Shifts, Jared Canonigo
How Acetylation Regulates Metabolic Enzyme Function During Environmental Shifts, Jared Canonigo
Biological Sciences Undergraduate Honors Theses
Organisms such as Saccharomyces cerevisiae can regulate the mechanisms of proteins through post-translational modification. These modifications play a vital role in functional proteomic activity because they can regulate protein activity, localization, and interaction with other cellular molecules. Such modifications include phosphorylation, methylation, and acetylation. The metabolic mechanisms of yeast became of keen interest to our lab because our lab noticed many stress defense proteins were being acetylated during stress heat shock. Notably, Adh1p and Adh2p showed both an increase and a decrease in acetylation at two lysine residues (K315 and K314) overtime during heat shock respectively, though the exact function …
Increasing Stress Tolerance In Saccharomyces Cerevisiae Using Lipocalin Genes, Ximena Leon
Increasing Stress Tolerance In Saccharomyces Cerevisiae Using Lipocalin Genes, Ximena Leon
Honors Theses
Saccharomyces cerevisiae is a microorganism that is commonly used in industries, but its productivity is significantly hindered by its inability to resist high stress levels found in industrial processes. By increasing stress tolerance in S. cerevisiae, its role in industrial processes could be maximized. The objective of this study is to determine if complementation of lipocalin genes into the wildtype and knockout strains of S. cerevisiae can increase stress tolerance against a variety of stressors. We identified the knockout yeast strains, ALD3, ALD4, PDX3, and ILV1, as being sensitive to various stressors including salinity, heat, oxidative, and osmotic stresses. Arabidopsis …