Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 3 of 3
Full-Text Articles in Life Sciences
Uracil Accumulation In Folate Depleted Mouse Embryonic Fibroblasts, Eneida Doko
Uracil Accumulation In Folate Depleted Mouse Embryonic Fibroblasts, Eneida Doko
Wayne State University Theses
Folate is a water-soluble vitamin B that plays a critical co-enzyme in the de novo nucleotide synthesis and other biochemical processes including DNA metabolism, DNA repair, DNA methylation, and cellular growth. Folate deficiency has been associated to increase the risk of neural tube defects (NTDs) and cancers of the lung, breast, colon, cervix, esposphagus and brain. Most importantly, folate deficiency has been shown to increase uracil misincorporation into DNA and therefore induce DNA damage repaired by the base excision repair (BER) pathway. In response to folate depletion, levels of thmidylate decrease in the deoxyribonucelotide pool, resulting in uracil being misincorporation …
Purification Of Alpha-Glucosidase Inhibitors From Grape Extract, Gayle M. Shipp
Purification Of Alpha-Glucosidase Inhibitors From Grape Extract, Gayle M. Shipp
Wayne State University Theses
ABSTRACT
PURIFICATION OF ALPHA-GLUCOSIDASE INHIBITORS FROM GRAPE EXTRACT
by
GAYLE SHIPP
August 2012
Advisor: Dr. Kevin Zhou
Major: Nutrition and Food Science
Degree: Master of Science
Diabetes and its complications are steadily growing and remain major causes of morbidity and mortality in the U.S. Intestinal á-glucosidases play a crucial role in controlling postprandial blood glucose. For this reason, one attractive prevention and/or treatment strategy for type-2 diabetes is the inhibition of á-glucosidase. The effectiveness of á-glucosidase inhibitors (AGI's) for diabetes treatment is well documented in numerous animal and human clinical studies. Grape pomace extract (GPE) has recently found in our …
The Effects Of Oil Palm Phenolics On Inflammation And Oxidative Stress In Relation To Amyloid Beta Plaques In Fad-Mutant And Wild Type B103 Cells, Lindsay Michelle Godsey
The Effects Of Oil Palm Phenolics On Inflammation And Oxidative Stress In Relation To Amyloid Beta Plaques In Fad-Mutant And Wild Type B103 Cells, Lindsay Michelle Godsey
Wayne State University Theses
Alzheimer's disease (AD) affects approximately 35.6 million people in the world today (35). It is expected that by year 2050, an individual will develop AD every 33 seconds (8). Unfortunately, the etiology is not well known. Familial Alzheimer's disease (FAD) occurs due to specific genetic mutations, making it possible to replicate them in cells. Using B103 rat neuroblastoma cells transfected with a cDNA construct, wild type cells and FAD mutant cells were used in this research.
Natural ingredients such as oil palm phenolics (OPP) contain antioxidants. After finding the optimal experimental parameters, OPP was found to aid in the destabilization …