Life Sciences Commons^™

Age Related Changes In The Microstructure Of Mozzarella Cheese, L. J. Kiely, P. S. Kindstedt, G. M. Hendricks, J. E. Levis, J. J. Yun, D. M. Barbano

Food Structure

Changes that occurred in the microstructure of low-moisture, part skim Mozzarella made with a mixed starter consisting of Streptococcus salivarius ssp. thermophilus and Lacwbacillus delbrueckii ssp. bulgaricus and coagulated with fermentation -produced chymosin , were examined during 50 days of ripening at 4 °C. Im mediately after manufacture (day 3), a homogeneous and continuous phase of amorphous paracasein represented a three-dimensional protein network in the cheese. A large number of irregularly shaped and sized microcavi ties were present. During 50 days of aging , an increase in the porosity of the defatted paracasein matrix was apparent. These changes were coincidental …

An Apparatus For A New Microcube Encapsulation Of Fluid Milk In Preparation For Transmission Electron Microscopy, M. C. Alleyne, D. J. Mcmahon, N. N. Youssef, S. Hekmat

Food Structure

A simple apparatus has been developed for a new "microcube" encapsulation of fluid milk samples in their prefixation preparation for electron microscopy. The new technique is based on making cubic wells in an agar gel layer, filling them with fluid milk samples, and sealing them with another agar gel layer . The individual wells are then separated by cutting from the initial block, providing 0.5 mm walls around the samples. The embedded material (milk, buttermilk, yogurt, etc.) is fixed, dehydrated , and embedded in a resin for transmission electron microscopy. The procedure is simpler, and more versatile, reliable, and reproducible …

Microstructure Of Black, Green And Red Gram, Enamuthu Joseph, Shelly G. Crites, Barry G. Swanson

Food Structure

The three most commonly consumed legumes (grams or pulses) in India, black gram (Vigna mungo (L.) Hepper), or urd , green gram (Vigna radiata (L.) Wilczek) or mung, and red gram (Cajanus cajan (L.) Millsp.) or tur, were examined by scanning electron microscopy . Seed coat and internal features were examined to differentiate these legumes from common beans (Phaseolus vulgaris) , adzuki beans (Vigna angu/aris) and lentils (Lens culinaris). Cross-sections of the seed coats of black, green and red gram contained single layers of columnar palisade cells extending as double palisade layers at the hilum regions. The funiculi located above …

Structure And Composition Of Apple Juice Haze, T. Beveridge, V. Tait

Food Structure

Haze obtained from commercial apple juice over the 1991 season contained from 11.4 to 29.0% protein (w/w) , trace quantities of both metal cations and polymeric carbohydrate, and gave strong responses to tests for phenolic compounds indicating a protein-phenol haze. SDS-PAGE of the 2-mercaptoethanol reduced , guanidinium hydrochloride dissaggregated haze particles gave a continuous smear indicating a population of molecules ranging from 29K to greater than 205K daltons. Transmission electron microscopy of negatively stained preparations revealed the presence of spherical bodies only partially penetrable by stain and possessin g a suhunit structure. These particles were embedded in a material presumably …

The Surface Coverage Of Fat On Food Powders Analyzed By Esca (Electron Spectroscopy For Chemical Analysis), Pia Faldt, Bjorn Bergenstahl, Gilbert Carlsson

Food Structure

ES.A (electron spectroscopy for chemical analysis) was used to estimate the fat coverage on different spray-dried food powder surfaces. The method presented here represents a new way of estimating the actual surface coverage of fat on a food powder. The ESCAmethod is illustrated with three different series of experimen ts. The results obtained with the ESCA-technique are combined with the results obtained from the conventional free fat extraction technique for different spray-dried powders.

In the first series , emulsion s containing different ratios of protein to fat were spray-dried. An increase in the amount of fat in the emulsion gives …

Development Of The Food Microscopist, D. F. Lewis

Food Structure

This paper describes the processes through which the food microscopist develops his/her skills. It is based around a framework of Shakespeare's seven ages of man. The first stage is learning about equipment: how it works and how not to abuse it. Next, attention turns to how to prepare samples for examination whilst maintaining the validity of the observations and avoiding damage to the instrument. The "Lover" is the time at which an understanding of the basic structures of food products is gained and the "Soldier " starts to interpret these structures in terms of the performance of the foodstuff. Next, …

The Stabilisation Of Air In Cake Batters - The Role Of Fat, B. E. Brooker

Food Structure

The role of fat crystals in the stabilisation of air bubbles in cake batters was studied with aqueous phase aerations prepared by the "all in one" method using shortening containing emulsifier. During mixing, fat crystals become coated with an interfacial layer of ad sorbed protein (crystal -water interface). They adsorb to the surface of bubbles (that have been transiently stabilised by egg proteins) by a process which involves the fu sion of the crystal-water interface with the air-water interface.

The adsorption of fa t crystals helps to stabilise large numbers of small bubbles which must expand without rupturing during baking …

Relationship Between The Starch Granule Structure And The Textural Properties Of Heat-Induced Surimi Gels, V. Verrez-Bagnis, B. Bouchet, D. J. Gallant

Food Structure

Starch, used as a textural additive in heat-induced surimi gel, influences the rigidity of the protein gel matrix and hence the gel strength according to its botanical characteristics. The present study focuses on the correlations existing between the textural properties of heatind uced surimi gels obtained by physical measurements and the characteristics of different commercial starches. The gelatinization temperature of starch was closely related to the expressible moisture, work to fracture, and elongation. Behaviour of starchy components during thermal processing and its relationship to fish protein gel matrix were studied by light and electron microscopy. These studies showed differences in …

Structure And Function Of Food Products: A Review, I. Heertje

Food Structure

A proper understanding of the behavior of food products requires knowledge of its structure, i.e., the spatial arrangement of the various structural elements and their interactions. The structure can properly be studied by visual observation techniques. In products such as fat spreads, creams, dressings, cheese, bread , milk , yoghurt , whipped cream, and ice cream , different structural elements can be distinguished. A number of those elements a re discussed, viz. , water droplets, oil droplets, gas cell s, particles, fat crystals and strand s. In addition examples of interactions between structural element s are presented, viz., oil droplets/matrix, …

Hydrocolloids As Food Emulsifiers And Stabilizers, Nissim Garti, Dov Reichman

Food Structure

Hydrocolloids are water-soluble biopolymers consisting of high molecular weight polysaccharides known as viscosity builders, gelification agents and stabilizers of food systems.

Several hydrocolloids such as gum arabic (acacia), tragacanth, xanthan and certain modified gums have been mentioned as food additives having special functions such as: "retardation of precipitation of dispersed solid particles and coalescence of oil droplets". The role of these gums as emulsifiers remained somewhat obscure. The present review is an attempt to bring the relevant studies together and to throw some light on the functionalities of the gums as surface active agents and food emulsifiers. In addition, some …

Changes In The Microstructure Of A Comminuted Meat System During Heating, Peter A. Koolmees, Gerrit Wijngaards, Monique H.G. Tersteeg, Jan G. Van Logtestijn

Food Structure

As part of a series of experiments on heating of a comminuted meat system , image analyses were conducted to quantify microstructural changes that could be visualized by light microscopy. Three different batches of comminuted meat were investigated. Two batches (A and B) were prepared without polyphosphate; one of these (B) was deliberately overchopped. One batch containing 0. I% polyphosphate was prepared (C). All batches were heated at I I different temperatures ranging from 40 to 115 °C. The microstructure of the three types of meat batte rs at the end of the com minu tion process differed. These types …

High-Resolution Scanning Electron Microscopy Of Milk Products: A New Sample Preparation Procedure, William R. Mcmanus, Donald J. Mcmahon, Craig J. Oberg

Food Structure

A metal-impregnation technique (tannin-ferrocyanide- osmium tetroxide) and thin metal coating imparted excellent preservation , and electric and thermal properties to milk products for scanning electron microscopy, resulting in high- resolution images. Three nanometer resolution was obtained, with minimal edge effects, charging , thermal drift, and decoration. Samples were fixed in 2% gluta raldehyde, cryoprotected in 70% ethanol, and cryofractured, exposing internal structure. Fat was extracted with Freon 113 and the sample were metal -impregnated with 1.0% osmium tetroxide and 1.5% potassium ferrocyanide, reduced with 1% hydroquinone, dried using !he critical-point drying melhod , and ion beam sputter-coated with 2-5 nm …