Life Sciences Commons^™

Effect Of Endophytic Fungi On Thrips Tabaci Development And Population Dynamics, Ngoc Anh Vu, Sevgan Subramanian, Rainer Meyhöfer

All Peer-Reviewed Publications

Endophytic fungi are promising biocontrol agents because they colonise healthy plant tissues asymptomatically while inducing systemic resistance that negatively affects herbivorous insects. We investigated whether treatments with the endophytes Trichoderma harzianum and Beauveria bassiana in two onion cultivars (Allium cepa L.; Sturon and Red Baron) induce plant responses that alter Thrips tabaci behaviour and population development. Under controlled conditions, seeds were treated with conidial suspensions and plants subsequently received foliar applications; plants were then exposed to T. tabaci for 72 h, 4 weeks, or 8 weeks in no-choice assays. Both endophytes reduced thrips host-plant preference: at 72 h post-exposure, inoculated …

Molecular Detection Of Campylobacter Spp. In Filth Flies Associated With Broiler Breeder Facilities In Oklahoma And Arkansas, Allen Szalanski

Entomology and Plant Pathology Faculty Publications and Presentations

Adult filth flies (Diptera: Muscidae) (non-biting flies that develop in decaying organic matter) are abundant in poultry facilities and may contribute to the dissemination of pathogenic Campylobacter spp. among flocks. We sampled adult house flies, Musca domestica L., and black dump flies, Hydrotaea aenescens (Wiedemann), at five broiler-breeder farms in Oklahoma and Arkansas to determine the prevalence of Campylobacter spp. vectored by filth flies and the effects of farm, fly species, and location (inside vs outside houses) on the rates of Campylobacter spp. occurrence. A total of 1,076 house flies and 1,008 black dump flies were collected inside and outside …

Tick Lipocalin Triggers Mammalian Igfbp-3-Mediated Apoptosis In Macrophages And Keratinocytes, Krittika Nandy, P. P. Mahesh, Lichao Liu, Daniel E. Sonenshine, Hameeda Sultana, Girish Neelakanta

Biological Sciences Faculty Publications

Ticks secrete several molecules, including lipocalins, in their saliva during blood feeding on a vertebrate host. In this study, we provide novel evidence on the role of Ornithodoros turicata americanus tick lipocalin (Otlip) in modulating cytokine expression and in triggering apoptosis in mammalian macrophages and keratinocytes. The cytokine array analysis revealed significantly increased secretion of insulin-like growth factor-binding protein 3 (IGFBP3) from murine macrophage cell line upon treatment with recombinant Otlip protein (rGST-Otlip) when compared to the secretion noted from cells treated with a control protein (rGST). Similar observation with cytokine protein array analysis was noted when murine macrophages were …

Herbivore Identity And Species Richness Shape Fruit Chemical And Quality Responses To Foliar Herbivory, Xavier Ozowara, Taylor M. Sloey, Susan R. Whitehead

Biological Sciences Faculty Publications

The cascading consequences of foliar herbivory on fruit chemistry and quality remain relatively underexplored, with most existing studies focusing on the effects of only one or two attackers. In this study, we examine how increasing herbivore species richness during foliar herbivory alters strawberry (Fragaria × ananassa Duch) fruit quality and phenolic chemistry. We conducted a greenhouse experiment with single species feeding and different combinations of feeding by three generalist lepidopteran species: Spodoptera frugiperda, Heliothis virescens, Helicoverpa zea. Species fed on leaves for 72 h as fruits ripened, and fully ripe fruits were harvested 72 h after …

Effects Of Rnai Of Ribophorin 1, Eukaryotic Translation Initiation Factor 2 Beta, And Peptidylprolyl Isomerase A In Acyrthosiphon Pisum, Brandon L. Kennemer

Master's Theses or Doctor of Nursing Practice

Three genes found in the Unfolded protein response, Ribophorin 1, Eukaryotic translation initiation factor 2 beta, and Peptidylprolyl isomerase A are thought to be potential targets for RNA Interference (RNAi) in Acyrthosiphon pisum.  Ribophorin 1 (RPN1) is a transmembrane glycoprotein that assists in anchoring ribosomes to the rough endoplasmic reticulum membrane.  It acts as a substrate-specific chaperone.  It facilitates N-glycosylation by delivering newly synthesized proteins to the oligosaccharyltransferase (OST) complex.  Eukaryotic translation initiation factor 2 beta (eIF2B) is a key component of the eIF2 heterotrimer that facilitates protein synthesis initiation by binding GTP and recruiting a specific transfer RNA to …

Bayesian Multivariate Modelling Of Lone Star (Amblyomma Americanum) Tick Life Stage Abundance And Temporal Trends To Inform Public Health Risk In Virginia, Thabo Lephoto, Henry Mwambi, Oliver Bodhlyera, Holly Gaff

Biological Sciences Faculty Publications

The increasing abundance of ticks poses a growing public health concern due to heightened human exposure to tick bites. The lone star tick (Amblyomma americanum: Ixodida: Ixodidae), a common human-biting species in the United States, has expanded its range in recent years. However, how its different life stages vary across time, habitats, and locations remains insufficiently understood. We analyzed tick abundance data collected in southeastern Virginia between 2009 and 2018, focusing on larval, nymphal, and adult life stages. A Bayesian multivariate modelling framework was used to examine seasonal patterns, habitat effects, spatial variation, and biological links between life stages. Two …

Optimizing Insect Exclusion Systems For Organic Specialty Crop Production, Elaine Losekamp

Theses and Dissertations--Entomology

Feeding damage from insect pests is a leading challenge for specialty crop growers who follow organic practices. Past research has shown that fine-mesh row covers installed over mesotunnel structures, which form a physical barrier between plants and insects, often reduce insect pest abundance and increase yield compared to open field conditions. However, there are several challenges associated with this practice. Exclusion efficacy varies across a spectrum of insect body sizes and mesh sizes, and effects on yield vary across crops. Additionally, pollination management for pollinator-dependent crops introduces labor requirements and may result in reduced pest exclusion efficacy. Mechanical weed management …

Seasonal Population Dynamics And Interspecific Interactions Of Drosophila Suzukii, Eleanor Mccabe

Theses and Dissertations--Entomology

Invasive species are a growing problem, and it is important to investigate how invasives interact with novel ecosystems, which are characterized by distinct abiotic and biotic factors. In this dissertation, I investigate abiotic and biotic factors affecting two invasive pests in central Kentucky, Drosophila suzukii and Zaprionus indianus. In Kentucky, seasonality, and landscape features on population dynamics and fitness are unknown. I found that D. suzukii overwinters in Kentucky and that wild berry species improve winter survival. I used local weather data to identify meteorological variables that best predict D. suzukii populations. I tested the extent to which D. suzukii …

County-Level Surveillance For The American Dog Tick (Dermacentor Variabilis) And Rickettsia Species In Kentucky, Callista W. Vandegriff, Jun Seok Ryoo, Maria C. Carrasquilla, Anna Rosalee Pasternak, Wayne T. Sanderson, Subba R. Palli

Entomology Faculty Publications

Spotted fever group (SFG) rickettsiosis includes a group of illnesses with similar symptoms caused by Rickettsia species bacteria. While Rickettsia rickettsii, the agent of Rocky Mountain spotted fever (RMSF) is a well-known pathogen, other SFG Rickettsia spp. can infect ticks and occasionally contribute to human disease. We conducted county-level surveillance of Dermacentor variabilis ticks in Kentucky to characterize the distribution and diversity of SFG Rickettsia. Ticks were collected from the environment from 2019 to 2024 by dragging a white cloth and through our Kentucky Tick Submission Program. In total, 2,023 D. variabilis ticks were collected from 114 …

Clinical Manifestations Of Bed Bug Bites: A Systematic Review Of Case Reports, Julian Felipe Porras-Villamil, Zachary C. Devries

Entomology Faculty Publications

Importance: Clinical characterization of bed bug (Cimex spp.) bite manifestations remains limited.   Objective: This systematic review synthesizes case reports to describe dermatological, systemic, and psychological outcomes of bed bug bites.   Evidence review: Following PRISMA guidelines, multiple databases, including Pubmed and Scopus, were searched using terms such as “bed bug,” “Cimex lectularius,” “Cimex hemipterus,” “case report,” and “case series.” Extracted data included demographics, species, lesion type, anatomical distribution, and clinical manifestations. Quantitative and qualitative analyses were conducted.   Results: Eighty-four patients were included: 76 with C. lectularius (90.5%) and, 8 with C. hemipterus (9.5%). Sex distribution was …

Complex Interplay Between Facilitation And Competition In Two Invasive Fruit Pests, Eleanor Mccabe, Nicholas M. Teets

Entomology Faculty Publications

When one invasive species facilitates the invasion of another species, it can lead to invasional meltdown. However, when the two species depend on the same finite resource, facilitation and competition can occur simultaneously and impact population dynamics. Furthermore, factors like resource availability and population density could alter the balance between facilitation and competition. Drosophila suzukii and Zaprionus indianus are two invasive insect pests that infest small fruit crops. The co-localization of these species in the same fields, coupled with the fact that Z. indianus lacks a hardened ovipositor that can penetrate the skin of fruit, has led to the hypothesis …

Elevated Temperatures Disrupt Wolbachia-Induced Feminisation And Reshape Microbial Community Dynamics Across Generations In A Spider Host, Virginija Mackevicius-Dubickaja, Jennifer A. White, Ellen E. Williams, Eyal Klement, Yuval Gottlieb, Matthew R. Doremus

Entomology Faculty Publications

Longitudinal microbial interactions within a host are challenging to study, leading to a focus on constructed microbial communities in vitro settings. Here, we take advantage of a naturally defined microbial community within a spider host to study how elevated temperatures influence microbial dynamics and phenotypes across host generations. The spider Mermessus fradeorum hosts up to five endosymbionts, including a Wolbachia strain, W1, which induces feminisation, causing genetic males to develop as phenotypic females, skewing sex ratios and promoting symbiont spread. Despite this, Wolbachia 1 persists at intermediate frequencies in wild populations. We hypothesised that elevated temperatures might reduce penetration of …

Evolutionary Transitions In Social Behavior Are Associated With Convergent And Partially Reversible Expansions Of Transcription Factor Binding Sites, Savanna G. Ploessl, Beryl M. Jones

Entomology Faculty Publications

The evolution of sociality involves shifts in physiology and behavior, most notably the emergence of a reproductive division of labor. Within social colonies, these distinct behavioral phenotypes arise from differential regulation of a shared genome. Changes in transcription factor (TF) binding motifs are one potential mechanism underpinning this plasticity, with prior studies suggesting that social species exhibit expansions in TF binding sites. However, it remains unclear whether motif expansions are reversed when sociality is lost. Here we analyze predicted TF motif occurrences across gene promoters in 42 bee species spanning millions of years of evolutionary divergence and multiple independent gains …

Characterizing Gut Microbial Communities And Symbiont-Mediated Detoxification In Herbivorous Insects, Rachel C. Vargas

Biology Dissertations

Insect gut bacteria are vital contributors to host physiology, yet the broader prevalence and fitness implications of symbiont-mediated detoxification remain poorly understood. This thesis investigates these dynamics across three research chapters. Chapter 1 uses 16S rRNA sequencing to characterize how grasshopper gut microbial communities vary across species, dietary breadths, and gastrointestinal segments. Chapter 2 evaluates the detoxification capacity of over 150 bacterial isolates from diverse herbivores against the plant allelochemicals phenethyl isothiocyanate, rutin, and chlorogenic acid to determine if host dietary specialization predicts microbial metabolic ability. Finally, Chapter 3 employs fitness bioassays to test whether inoculating generalist herbivores with detoxifying …

Ovipositional Behavior Of The Twig Girdler (Cerambycidae: Oncideres Cingulata) On Stems Of Mockernut Hickory (Carya Tomentosa) In Arkansas, R. Tumlison, L. Serviss

Journal of the Arkansas Academy of Science

The Twig Girdler (Cerambycidae: Oncideres cingulata) is a long-horned beetle that girdles stems of certain species of trees to prepare a habitat for its offspring. The stems eventually die, break, and fall from the tree, but the beetle usually had already laid its eggs within the stems. Eggs are laid singly, almost always at growth rings or leaf bases. Because the space for the young to develop is finite, laying too many eggs could cause reproductive failure and too few eggs would be suboptimal. We hypothesized that the female laying eggs should be able to evaluate the stem and …

Technology-Driven Approaches For Invasive Species Detection And Management, Kushal Naharki

Graduate Theses, Dissertations, and Problem Reports (ETD)

Invasive species pose significant ecological, economic, and management challenges globally. Purple loosestrife (Lythrum salicaria), Japanese knotweed (Fallopia japonica), tree of heaven (Ailanthus altissima), and mile-a-minute (Persicaria perfoliata) are widespread invasive plants across the eastern United States and present persistent challenges, particularly in remote and inaccessible landscapes. Effective management of these species requires an integrated approach that combines early detection, targeted intervention, public engagement, and spatial modeling. This dissertation used emerging technologies to improve invasive species detection, monitoring, and management.

Unmanned aerial vehicle (UAV)-based platforms equipped with optical sensors were used to establish …

Genetics Of Mycangial Development And Host Colonization Within The Ambrosia Symbiosis, Mckeon James Laws

Graduate Theses, Dissertations, and Problem Reports (ETD)

Ambrosia beetles, a polyphyletic group of weevils, utilize pouch-like structures at various locations on the body to transport for their sole food source, their fungal partner. Ambrosia fungi are known to originate from multiple unrelated fungal lineages with no consensus of traits required to become a fungal cultivar. While the biology and ecology of both partners is in many cases well understood, the genetic underpinnings of this fascinating mutualism remain a mystery. The aim of this dissertation is to add to the understanding of the ambrosia symbiosis by providing insights into its molecular and genetic underpinnings through RNA sequencing data, …

Relationships Between Benthic Macroinvertebrate Communities And Environmental Conditions In Headwater Tributaries Of The Pend Oreille Lake Watershed, Idaho, Devlin Mee

EWU Masters Thesis Collection

Benthic macroinvertebrates are widely used indicators of stream condition, and their communities respond to thermal and land-use conditions in headwater streams. This study characterized macroinvertebrate biomass, community composition, and diversity across forested headwater tributaries of the Pend Oreille lake watershed in northern Idaho, and examined their relationships with summer temperature, land development, and road proximity. Samples from thirteen streams collected in 2024 were identified to genus, measured, and converted to estimated biomass using published length–mass regressions; ten streams had recovered temperature loggers. Because environmental variables were characterized once per stream, all analyses were conducted at the stream level.

Estimated total …

Letter From The Faculty Editor, Beth Kegley

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Featured Faculty Mentor/Student Team, Lauren Rogers, Danielle Reeves

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Evaluating Benthic Macroinvertebrate Communities In Streamside Management Zones In Private, Working Forests, Haley Vaglienti

College of Graduate Studies: Theses & Dissertations

Forestry operations can alter riparian vegetation and increase sediment and nutrient inputs to streams, affecting water quality and aquatic biota. Forestry best management practices (BMPs) including streamside management zones (SMZs) are designed to mitigate those effects by reducing sediment delivery and moderating temperature, flow, and nutrient regimes. We assessed ecological integrity at 24 intermittent stream sites with SMZs in private, working forests across the Piedmont and Southeastern plains of Georgia. At each site, we conducted physical habitat evaluations and characterized benthic macroinvertebrate assemblages. Sites represented a gradient of habitat conditions, SMZ widths, and pine stand ages, allowing for comparisons of …

Dna Barcoding Complements Citizen Science Identifications In An Arthropod Survey At Governors Island, New York, Malky Gestetner

Dissertations and Theses

Arthropods are declining worldwide, threatened by habitat loss, climate change, and growing urbanization. Efforts to monitor arthropod biodiversity are crucial for conservation policies and for maintaining the ecosystem services provided by these animals in urban environments. Surveys of arthropods have been attempted in many New York neighborhoods, but no formal assessment has been conducted on Governors Island, which harbors 72-acres of parkland within a short boat ride away from Manhattan. This is a deficiency which this study helps to address. Following student-led collection efforts within four Governors Island sites, DNA barcoding was employed to complement specimen identification based on morphology …

Behavioral And Electrophysiological Responses Of Fall Armyworm Larval Parasitoid Coccygidium Luteum To Maize And Companion Plant Volatiles, Emmanuel Peter, Sevgan Subramanian, Abdullahi Yusuf, Samira A. Mohamed, Baldwyn Torto, Emily A. Martin, Amanuel Tamiru

All Peer-Reviewed Publications

Companion cropping and biological control are among the best agronomic practices recommended for ecologically sustainable management of the invasive fall armyworm (FAW), Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae). Companion plants may suppress the FAW population by repelling the pest, disrupting host-plant location and recruiting biocontrol agents such as parasitoids. These interactions are primarily mediated by plant-derived volatile organic compounds (VOCs). Understanding parasitoids’ responses to VOCs emitted by companion plants enables selection of suitable cultivars to enhance biological control and design appropriate intercropping systems for effective FAW management. This study investigated behavioral and electrophysiological responses of a key FAW larval parasitoid, …

Letter From The Dean, Jeff Edwards

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Instructions For Authors, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Fumigant Toxicity Of Essential Oils From Chemotypes Of Curry Tree (Murraya Koenigii) Against Adult African Malaria Mosquito (Anopheles Gambiae Sensu Stricto), Clarence M. Mang’Era, Fathiya M. Khamis, Ahmed Hassanali, Paul O. Mireji

All Peer-Reviewed Publications

The toxicity of fumigant formulations of essential oils (EOs) from curry tree (Murraya koenigii (L.) Spreng (Rutaceae)) against African malaria adult mosquito (Anopheles gambiae sensu stricto (Giles, 1902) (Diptera: Culicidae: Culicini)) was evaluated to identify most effective chemotype of the curry tree against the mosquitoes. Ninety-two compounds (91.78%–96.47% coverage) in the EO profiles were identified, among which EO isolated from curry tree chemotype from Kibwezi was most toxic. This chemotype had a median lethal dose (LD50) of 3.6 × 10⁻³ mg/cm³ (95% CI: 2.70–4.58) and a lethal time (Ti50) of 1.91 ± 0.086 h (p < 0.001). The equivalent LD50 of EOs of chemotypes from Mombasa, Makindu, and Malindi in Kenya was 4.9 × 10⁻³, 6.6 …

Cytonuclear Incompatibilities In Hybrid Systems: Oxidative Phosphorylation Capacity And Nuclear Marker Transmission Ratio Distortion In Hybrids Of An Introgressed Strain Of Parasitoid Wasp, Julie Kessler

College of Graduate Studies: Theses & Dissertations

Speciation is an evolutionary process resulting in the divergence of populations into reproductively isolated species, a key concept that supplies our planet with an abundance of biodiversity. Mechanisms for speciation center on obstacles to fertilization and include post-zygotic barriers, such as intrinsic deleterious interactions, that arise in hybrid systems where genetic variants are combined. Genes from separate parent lineages are often incompatible due to the co-evolution of paired genes within a singular species not matching their complement genes in another species, and the result is a reduction in fitness of the hybrid when these genes come together. The mitochondrial OXPHOS …

Effects Of Temperature On The Life-History Traits Of Myzus Persicae And Its Efficiency In Transmitting Potato Virus Y (Pvy) In Potato Crops, Bonoukpoè M. Sokame, Henri E. Tonnang, Heidy Gamarra, Pablo Carhuapoma, Jan Kreuze, Leah R. Johnson, Ali Arab, Peter A. Armbruster, Oswaldo C. Villena

All Peer-Reviewed Publications

Aphids are highly sensitive to temperature changes and play a crucial role in transmitting plant viruses, accounting for the transmission of more than 50% of viruses that cause disease in crops. Among them, Myzus persicae is a major global pest, affecting over 400 plant species and transmitting more than 100 plant viruses, including potato virus Y (PVY), which poses a severe threat to potato crops. This study examines how temperature influences the life-history traits of M. persicae and its efficiency in transmitting PVY. Our research revealed that temperature significantly affects developmental duration, survival, and fecundity of M. persicae. The aphids …

Omega-3 Fatty Acids And Bioactives In Aqueous-Extracted Oils From Seven Edible Insects, Xavier Cheseto, Chrysantus Mbi Tanga

All Peer-Reviewed Publications

Globally, there is urgent search and research interest towards alternative sources of polyunsaturated omega-3 fatty acids (ω-3 FA) due to sustainability challenges faced by dependence on fish and plant-derived sources. Limited information exists on insects despite their promising potential. This work analyzed oils from seven insect species commonly consumed globally compared to commercial sesame oil using coupled gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS). Oil yields from insects ranged between 11.1 mg/g to 115.3 mg/g. Over 67 fatty acids, α-tocopherol [vitamin E] and eight flavonoids were identified. All insect oils contained ω-3 FA [i.e., α-linolenic acid (ALA), eicosapentaenoic …

Thermal Requirements And Phenology Modelling Of A Drosophila Suzukii Population: Implications For Pest Risk Under A Changing Climate, Shepard Ndlela, Abdelmutalab G.A. Azrag, Esther Owino Awuor, Ibrahim Maholidy Farid, Samira Abuelgasim Mohamed

All Peer-Reviewed Publications

Drosophila suzukii (spotted-wing drosophila) is an invasive pest causing significant economic losses in soft-skinned fruit crops globally. This study presents comprehensive temperature-dependent life table data and phenology models for an African D. suzukii population collected in Kenya. Developmental time, mortality, fecundity, and adult longevity were assessed under constant temperatures from 12 to 30 °C. Using Insect Life Cycle Modelling (ILCYM) software, linear and nonlinear models were applied to characterise the effects of temperature on life history traits. Additionally, spatial risk indices including Establishment Risk Index (ERI), Generation Index (GI), and Activity Index (AI) were mapped globally for current and future …