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Full-Text Articles in Life Sciences
Assessment Of Non-Saccharomyces Yeast Species As Secondary Fermenters In Beer, Paul J. Clark
Assessment Of Non-Saccharomyces Yeast Species As Secondary Fermenters In Beer, Paul J. Clark
Master of Science in Integrative Biology Theses
Secondary fermentation of beer is traditionally used to condition and refine the sensory profile of a beer. During methods such as barrel aging or lagering, the yeast continues to produce flavor-active compounds. Some methods include the intentional inoculation of microorganisms or the utilization of natural consortia associated with a barrel, fruit, or additive for the generation of desired sensory profiles. As unique products increase in popularity, brewers have begun to experiment with secondary fermentation using alternative yeast species as bioflavorants. To assess the viability of an organism’s application to brewing, the physiochemical properties of beer and their impact on the …
How Acetylation Regulates Metabolic Enzyme Function During Environmental Shifts, Jared Canonigo
How Acetylation Regulates Metabolic Enzyme Function During Environmental Shifts, Jared Canonigo
Biological Sciences Undergraduate Honors Theses
Organisms such as Saccharomyces cerevisiae can regulate the mechanisms of proteins through post-translational modification. These modifications play a vital role in functional proteomic activity because they can regulate protein activity, localization, and interaction with other cellular molecules. Such modifications include phosphorylation, methylation, and acetylation. The metabolic mechanisms of yeast became of keen interest to our lab because our lab noticed many stress defense proteins were being acetylated during stress heat shock. Notably, Adh1p and Adh2p showed both an increase and a decrease in acetylation at two lysine residues (K315 and K314) overtime during heat shock respectively, though the exact function …
Mapping Genetic Variants Associated With Dynamic Protein Abundance In Haploid Yeast, Tanner Thuet-Davenport
Mapping Genetic Variants Associated With Dynamic Protein Abundance In Haploid Yeast, Tanner Thuet-Davenport
WWU Graduate School Collection
As organisms respond to changes in their environment genetic variation between individuals can directly affect organismal trait phenotypes by altering gene expression. Historically, studies have focused on the effect of genetic variation on mRNA synthesis (transcription) and decay rates. Relatively few studies have probed the relationship between DNA variants and protein-specific regulation of individual genes, despite the plethora of evidence that RNA levels are often poor proxies for protein levels. No study to date has mapped genetic variation associated with dynamic protein levels. In this study we investigated the location and identity of genetic variants acting on protein expression dynamics …