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Dynamin Association With Clathrin And Its Physiological Roles At The Golgi And Targeting Mechanism To The Golgi, Shiva Kumar Goud Gadila
Dynamin Association With Clathrin And Its Physiological Roles At The Golgi And Targeting Mechanism To The Golgi, Shiva Kumar Goud Gadila
MSU Graduate Theses
Vps1 (Vacuole protein sorting 1), a dynamin-like protein in yeast, is implicated in diverse membrane trafficking pathways and localized to many organelles, including the Golgi. It has been proposed that Vps1 is functionally linked to clathrin heavy chain 1 (Chc1), but the question of how, where, and when they function together remains unknown. Lines of evidence suggest that Chc1 and Vps1 are located at the late Golgi. Therefore, I hypothesized that Vps1 binds to Chc1 and functions together at the Golgi for efficient Golgi-to-endosome membrane trafficking. Using the yeast-two hybrid assay, I present evidence that Vps1 binds to the C-terminal …
Functional Connection Between Yeast Dynamin And Retromer At The Endosome, Christopher Robert Trousdale
Functional Connection Between Yeast Dynamin And Retromer At The Endosome, Christopher Robert Trousdale
MSU Graduate Theses
Intracellular trafficking from the late endosome to Golgi in cells is termed retrograde transport, essential for recycling of important macromolecules including cell membrane receptors. Retrograde transport is regulated by a family of proteins known as the "Retromer” composed of 5 VPS proteins (Vps5, Vps17, Vps26, Vps29, and Vps35). Retromer acts as the coat proteins for vesicles emerging from late endosomes. Loss of Retromer function has been previously implicated in both Parkinson's and Alzheimer's disease. Vps1, a yeast dynamin-like protein, plays a role in intracellular trafficking. Vps1 has been shown to localize at the endocytic sites to promote pinching off of …