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Isolating Highly Specific Antibodies Against Cancer Target Hprt, Austin J. Hansen, Christopher J. Haynie, Kiara V. Whitley, Michelle Townsend, Abby Johnson, Hunter Lindsay, Laura Baird, Kim L. O'Neill
Isolating Highly Specific Antibodies Against Cancer Target Hprt, Austin J. Hansen, Christopher J. Haynie, Kiara V. Whitley, Michelle Townsend, Abby Johnson, Hunter Lindsay, Laura Baird, Kim L. O'Neill
Library/Life Sciences Undergraduate Poster Competition 2023
Effective immunotherapeutic targeting of cancer cells requires identification of specific antigenic differences between cancerous and healthy cells. Hypoxanthine-guanine phosphoribosyltransferase (HPRT) is a cytoplasmic enzyme involved in the salvage pathway for DNA synthesis. Certain cancers (e.g., prostate and breast) upregulate and express HPRT on the cell membrane, making HPRT a potential cancer biomarker for cancer immunotherapies. Antibodies specific for unique cancer proteins are critical to develop targeted cancer immunotherapies. Using a yeast display library and cell sorting, we selected for high-affinity human single-chain (scFv) antibodies against HPRT that can be utilized in chimeric antigen receptor (CAR) T cell therapies and antibody-dependent …