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Articles 271 - 297 of 297
Full-Text Articles in Life Sciences
Nodule Organogenesis And Symbiotic Mutants Of The Model Legume Lotus Japonicus, Krzysztof Szczyglowski, Robert S. Shaw, Judith Wopereis, Sue Copeland, Dirk Hamburger, Beth Kasiborski, Frank B. Dazzo, Frans J. De Bruijn
Nodule Organogenesis And Symbiotic Mutants Of The Model Legume Lotus Japonicus, Krzysztof Szczyglowski, Robert S. Shaw, Judith Wopereis, Sue Copeland, Dirk Hamburger, Beth Kasiborski, Frank B. Dazzo, Frans J. De Bruijn
Biological Sciences: Faculty Publications
A detailed microscopical analysis of the morphological features that distinguish different developmental stages of nodule organogenesis in wild-type Lotus japonicus ecotype Gifu B-129-S9 plants was performed, to provide the necessary framework for the evaluation of altered phenotypes of L. japonicus symbiotic mutants. Subsequently, chemical ethyl methanesulfonate (EMS) mutagenesis of L. japonicus was carried out. The analysis of approximately 3,000 M1 plants and their progeny yielded 20 stable L. japonicus symbiotic variants, consisting of at least 14 different symbiosis- associated loci or complementation groups. Moreover, a mutation affecting L. japonicus root development was identified that also conferred a hypernodulation response when …
Detection Of The Filarial Parasite Mansonella Streptocerca In Skin Biopsies By A Nested Polymerase Chain Reaction-Based Assay, Peter U. Fischer, Dietrich W. Büttner, Jotham Bamuhiiga, Steven A. Williams
Detection Of The Filarial Parasite Mansonella Streptocerca In Skin Biopsies By A Nested Polymerase Chain Reaction-Based Assay, Peter U. Fischer, Dietrich W. Büttner, Jotham Bamuhiiga, Steven A. Williams
Biological Sciences: Faculty Publications
To differentiate the skin-dwelling filariae Mansonella streptocerca and Onchocerca volvulus, a nested polymerase chain reaction (PCR) assay was developed from small amounts of parasite material present in skin biopsies. One nonspecific and one specific pair of primers were used to amplify the 5S rDNA spacer region of M. streptocerca. Biopsies with different microfilaria densities obtained from 104 Ugandans living in an area endemic forM. streptocerca were tested using both the nested PCR assay and standard parasitologic assessment of microfilariae. All 82 samples from microfilaria carriers were positive when tested using the nested PCR assay. In addition, M. streptocerca DNA could …
Effect Of Transatlantic Transport On Reproduction Of Agouti And Nonagouti Deer Mice, Peromyscus Maniculatus, Virginia Hayssen
Effect Of Transatlantic Transport On Reproduction Of Agouti And Nonagouti Deer Mice, Peromyscus Maniculatus, Virginia Hayssen
Biological Sciences: Faculty Publications
In conjunction with establishing colonies of deer mice in the UK, effects of transportation on reproduction in agouti (A) and nonagouti (a) deer mice were assessed. Adults were shipped via ground courier and air freight from Northampton, Massachusetts, USA to Sutton Bonington, Leicestershire, England in February and June. Deer mice were paired upon arrival in Sutton Bonington, whereas matched controls were paired in the original colonies at shipping. To assess reproduction, the following variables were monitored for 110 days for all 96 pairs: number of pairs producing litters, time from pairing to birth, interlitter interval, litter size at birth, and …
Interactive Effects Of Inducible Defense And Resource Availability On Phlorotannins In The North Atlantic Brown Alga Fucus Vesiculosus, P. Peckol, J. M. Krane, J. L. Yates
Interactive Effects Of Inducible Defense And Resource Availability On Phlorotannins In The North Atlantic Brown Alga Fucus Vesiculosus, P. Peckol, J. M. Krane, J. L. Yates
Biological Sciences: Faculty Publications
Research seeking to explain the ecological role of polyphenolics (phlorotannins) in plants and brown algae has largely focused on 2 alternative concepts, the carbon/nutrient (C/N) balance and the inducible defense models. We tested the hierarchy of effects of both models on phlorotannin production in the brown alga Fucus vesiculosus (Fucales) by simultaneously manipulating the N environment and simulating herbivory for 2 oceanic (high and low intertidal) and estuarine populations. We measured phlorotannin levels in algae under control, grazed, N-enriched, and grazed + N-enriched treatments with time (0 to 14 d) throughout the year to determine onset and duration of the …
A Pcr Assay For The Detection Of Wuchereria Bancrofti In Blood, Min Zhong, James Mccarthy, Lou Ann Bierwert, Michelle Lizotte-Waniewski, Suzanne Chanteau, Thomas Nutman, Eric A. Ottesen, Steven A. Williams
A Pcr Assay For The Detection Of Wuchereria Bancrofti In Blood, Min Zhong, James Mccarthy, Lou Ann Bierwert, Michelle Lizotte-Waniewski, Suzanne Chanteau, Thomas Nutman, Eric A. Ottesen, Steven A. Williams
Biological Sciences: Faculty Publications
To identify Wuchereria bancrofti DNA sequences that could be used as the basis for a simple and rapid parasite detection assay, a genomic library of W. bancrofti was constructed and screened for highly repeated DNA. The repeat found with the highest copy number was 195 basepairs (bps) long, 77% AT, and 300 copies per haploid genome. This sequence was designated the Ssp I repeat because it has a unique recognition site for that restriction endonuclease in all or most of the repeat copies. The Ssp I repeat DNA family is dispersed, genus-specific, and exists in all of the different geographic …
Allometry Of Litter Mass In Bats: Maternal Size, Wing Morphology, And Phylogeny, Virginia Hayssen, T. H. Kunz
Allometry Of Litter Mass In Bats: Maternal Size, Wing Morphology, And Phylogeny, Virginia Hayssen, T. H. Kunz
Biological Sciences: Faculty Publications
We examine how litter mass in bats varies with respect to wing loading, an important aerodynamic aspect of flight. From geometric proportions, litter mass should scale to wing loading by an exponent of three. Conversely, analysis of aerodynamic consequences of carrying extra mass suggests that an exponent significantly less than three would be selectively advantageous. Our results show that Megachiroptera and Microchiroptera differ in the relationship between litter mass and wing loading. Litter mass in megachiropterans scales as expected by geometric proportions, whereas litter mass in microchiropterans, as a group, and for individual families, scales as expected if aerodynamic consequences …
Identification And Localization Of Three Classes Of Myosins In Pollen Tubes Of Lilium Longiflorum And Nicotiana Alata, D. D. Miller, Stylianos P. Scordilis, P. K. Hepler
Identification And Localization Of Three Classes Of Myosins In Pollen Tubes Of Lilium Longiflorum And Nicotiana Alata, D. D. Miller, Stylianos P. Scordilis, P. K. Hepler
Biological Sciences: Faculty Publications
The presence and localization of actin and myosin have been examined in pollen tubes of Lilium longiflorum and Nicotiana alata. Immunoblot analysis of pollen tube extracts with antibodies to actin, myosins IA and IB, myosin II, and myosin V reveals the presence of these contractile proteins. Immunofluorescence microscopy using various methods to preserve the pollen tubes; chemical fixation, rapid freeze fixation and freeze substitution (RF-FS) followed by rehydration or by embeddment in a methacrylate mixture, was performed to optimize preservation. Immunocytochemistry reaffirmed that actin is localized longitudinally in the active streaming lanes and near the cortical surface of the …
Molecular Phylogenetic Studies Of The Genus Brugia, Hong Xie, O. Bain, Steven A. Williams
Molecular Phylogenetic Studies Of The Genus Brugia, Hong Xie, O. Bain, Steven A. Williams
Biological Sciences: Faculty Publications
This paper is the first molecular phylogenetic study on Brugia para- sites (family Onchocercidae) which includes 6 of the 10 species of this genus : B. beaveri Ash et Little, 1 9 6 4 ; B. buckleyiDissanaike et Paramananthan, 1961 ; B. malayi (Brug,1927) Buckley, 1960 ; B. pohangi, (Buckley et Edeson, 1956) Buckley, 1960; B. patei (Buckley, Nelson etHeisch,1958) Buckley, 1960 and B. limori Partono et al., 1 9 7 7 . Hha I repeat sequences are 322 nucleotides long, highly repeated, tandemly arranged and unique to the nuclear genomes of the genus Brugia. Hha I repeat sequence data …
Molecular Phylogenetic Studies On Filarial Parasites Based On 5s Ribosomal Spacer Sequences, Hong Xie, O. Bain, Steven A. Williams
Molecular Phylogenetic Studies On Filarial Parasites Based On 5s Ribosomal Spacer Sequences, Hong Xie, O. Bain, Steven A. Williams
Biological Sciences: Faculty Publications
This paper is the first large-scale molecular phylogenetic study on filarial parasites (family Onchocercidae) which includes 16 spe cies of 6 genera : Brugia beaveri Ash et Little, 1964 ; B. buckleyiDissanaike et Paramananthan, 1961 ; B. malayi (Brug, 1927) Buckley, 1960; B. pahangi (Buckley et Edeson, 1956) Buckley, 1960; B. patei (Buckley, Nelson et Heisch, 1958) Buckley, 1960; B. timori Partono etal, 1977; Wuchereria bancrofti(Cobbold, 1877) Seurat, 1921; W. kalimantani Palmieri , Purnomo, Dennis and Marwoto, 1980; Mansonella perstans(Manson, 1891 ) Eberhard et Orihel, 1 9 8 4 ; Loa loa, Stiles, 1905; Onchocerca volvulus (Leuckart, 1983) Railliet et …
Thermoregulation Of The Pap Operon: Evidence For The Involvement Of Rimj, The N-Terminal Acetylase Of Ribosomal Protein S5, C. A. White-Ziegler, D. A. Low
Thermoregulation Of The Pap Operon: Evidence For The Involvement Of Rimj, The N-Terminal Acetylase Of Ribosomal Protein S5, C. A. White-Ziegler, D. A. Low
Biological Sciences: Faculty Publications
Our previous work showed that pap pilin gene transcription is subject to a thermoregulatory control mechanism under which pap pilin is not transcribed at a low temperature (23°C) (L. B. Blyn, B. A. Braaten, C. A. White-Ziegler, D. H. Rolfson, and D. A. Low, EMBO J. 8:613-620, 1989). In order to isolate genes involved in this temperature regulation of gene expression, chromosomal mini-TnlO (mTnlO) mutations that allowed transcription of the pap pilin gene at 23°C were identified, and the locus was designated tcp, for "thermoregulatory control of pap" (C. A. White-Ziegler, L. B. Blyn, B. A. Braaten, and D. A. …
A Proline-Rich Structural Protein Of The Surface Sheath Of Larval Brugia Filarial Nematode Parasites, Murray E. Selkirk, Maria Yazdanbakhsh, Danie Freedman, Mark L. Blaxter, Edith Cookson, Rosalind E. Jenkins, Steven A. Williams
A Proline-Rich Structural Protein Of The Surface Sheath Of Larval Brugia Filarial Nematode Parasites, Murray E. Selkirk, Maria Yazdanbakhsh, Danie Freedman, Mark L. Blaxter, Edith Cookson, Rosalind E. Jenkins, Steven A. Williams
Biological Sciences: Faculty Publications
Both cDNA and genomic DNA sequences have been isolated which encode a proline-rich precursor protein of the sheath from microfilariae, the first stage larvae of the filarial nematode parasites Brugia pahangi andBrugia malayi. This 22-kDa protein is soluble only under reducing conditions and is extensively cross- linked by both disulfide and nonreducible bonds. Immunogold electron microscopy shows that the protein is localized exclusively in the sheath, a vestigial remnant of the eggshell, which is retained by and encloses the mature microfilaria. Analysis by Western blotting confirms that the protein is expressed only in microfilariae and adult female worms, although transcripts …
Identification Of An Escherichia Coli Genetic Locus Involved In Thermoregulation Of The Pap Operon, C. A. White-Ziegler, L. B. Blyn, B. A. Braaten, D. A. Low
Identification Of An Escherichia Coli Genetic Locus Involved In Thermoregulation Of The Pap Operon, C. A. White-Ziegler, L. B. Blyn, B. A. Braaten, D. A. Low
Biological Sciences: Faculty Publications
We previously showed, using a single-copy papBAp-lac fusion (previously designated papBA-lac), that pyelonephritis-associated pili (pap) pilin gene transcription is subject to both phase variation and thermoreg- ulatory control mechanisms (L. B. Blyn, B. A. Braaten, C. A. White-Ziegler, D. H. Rolfson, and D. A. Low, EMBO J. 8:613-620, 1989). At 37°C, Escherichia coli strains carrying the papBAp-lac fusion displayed both Lac' and Lac- colony phenotypes. In contrast, at 23°C, colonies displayed a uniform Lac- phenotype, suggesting that pilin was not transcribed at this temperature. In this study, a strain carrying the papBAp-lac fusion was subjected to mini-TnlO (mTnlO) mutagenesis to …
Direct Genomic Sequencing Of Bacterial Dna: The Pyruvate Kinase I Gene Of Escherichia Coli, Osamu Ohara, Robert L. Dorit, Walter Gilbert
Direct Genomic Sequencing Of Bacterial Dna: The Pyruvate Kinase I Gene Of Escherichia Coli, Osamu Ohara, Robert L. Dorit, Walter Gilbert
Biological Sciences: Faculty Publications
The genomic sequencing procedure is applied to the direct sequencing of uncharacterized regions of bacterial DNA by a "multiplex walking" approach. Samples of bulk Escherichia coli DNA are cut with various restriction enzymes, subjected to chemical sequencing degradations, run in a sequencing gel, and transferred to nylon membranes. When a labeled oligomer is hybridized to a membrane, a sequence ladder appears wherever the probe lies near a restriction cut. New probes, based on sequence that lies beyond other restriction sites, are then synthesized, and the membranes are reprobed to reveal new sequence. Repeated cycles of oligomer probe synthesis and subsequent …
One-Sided Polymerase Chain Reaction: The Amplification Of Cdna, Osamu Ohara, Robert L. Dorit, Walter Gilbert
One-Sided Polymerase Chain Reaction: The Amplification Of Cdna, Osamu Ohara, Robert L. Dorit, Walter Gilbert
Biological Sciences: Faculty Publications
We report a rapid technique, based on the polymerase chain reaction (PCR), for the direct targeting, enhancement, and sequencing of previously uncharacterized cDNAs. This method is not limited to previously sequenced transcripts, since it requires only two adjacent or partially overlapping specific primers from only one side of the region to be amplified. These primers can be located anywhere within the message. The specific primers are used in conjunction with nonspecific primers targeted either to the poly(A)+ region of the message or to an enzymatically synthesized d( A) tail. Pairwise combinations of specific and general primers allow for the amplification …
A Field Study Using The Polymerase Chain Reaction (Pcr) To Screen For Brugia Microfilariae In Human And Animal Blood, Janet Glover, Steven A. Williams, Susanne Szabo, David Landry, Larry A. Mcreynolds, Taniawati Supali, Felix Partono
A Field Study Using The Polymerase Chain Reaction (Pcr) To Screen For Brugia Microfilariae In Human And Animal Blood, Janet Glover, Steven A. Williams, Susanne Szabo, David Landry, Larry A. Mcreynolds, Taniawati Supali, Felix Partono
Biological Sciences: Faculty Publications
Blood samples from 43 humans and 14 cats with Brugia microfilariae were analyzed in a field study in Tanjung Pinang, Indonesia. The study used the polymerase chain reaction (PCR) to compare the sensitivity of radioactive and biotinylated species-specific oligonuleotide probes. The cloning char- acterization of the Hha I repeat DNA family found in filarial parasites of the genus Brugia, and the development of species-specific probes for B.malayi and B.pahangi based on these repeats has been described elsewhere (PNAS USA 83: 797-801); Mol.Biochem. Parasitol. 28: 163-170). The use of radioisotopes for labelling DNA probes is both expensive and inconvenient. To replace …
Phase-Variation Of Pyelonephritis-Associated Pili In Escherichia Coli: Evidence For Transcriptional Regulation, L. B. Blyn, B. A. Braaten, C. A. White-Ziegler, D. H. Rolfson, D. A. Low
Phase-Variation Of Pyelonephritis-Associated Pili In Escherichia Coli: Evidence For Transcriptional Regulation, L. B. Blyn, B. A. Braaten, C. A. White-Ziegler, D. H. Rolfson, D. A. Low
Biological Sciences: Faculty Publications
The regulation of pyelonephritis-associated pill (pap) pflin gene transcription has been examined using two operons (pap-17 and pap-21) isolated from the pyelonephritogenic Escherichia coli strain C1212. DNA sequence analysis and E.coli minicell analysis were used to map two genes (papB and papl) within the pilin regulatory regions of both pap-17 and pap-21, and the protein products of these genes were identified. Pilin transcription, initiated at the papBA promoter, was monitored by constructing single copy operon fusions with lacZYA in E.coli K-12. Inocula- tion of E.coli (pap'-lac) strains onto solid M9 minimal medium containing glycerol and the Lac indicator X-gal (M9-Glycerol) …
The Control Of Myocardial Contraction With Skeletal Fast Muscle Troponin C., A. Babu, Stylianos P. Scordilis, E. H. Sonnenblick, J. Gulati
The Control Of Myocardial Contraction With Skeletal Fast Muscle Troponin C., A. Babu, Stylianos P. Scordilis, E. H. Sonnenblick, J. Gulati
Biological Sciences: Faculty Publications
The present study describes experiments on the myocardial trabeculae from the right ventricle of Syrian hamsters whose troponin C (TnC) moiety was exchanged with heterologous TnC from fast skeletal muscle of the rabbit. These experiments were designed to help define the role of the various classes of Ca2+-binding sites on TnC in setting the characteristic sensitivities for activations of cardiac and skeletal muscles. Thin trabeculae were skinned and about 75% of their troponin C extracted by chemical treatment. Tension development on activations by Ca2+ and Sr2+ was found to be nearly fully blocked in such TnC extracted preparations. Troponin C …
Cloning And Comparison Of Repeated Dna Sequences From The Human Filarial Parasite Brugia Malayi And The Animal Parasite Brugia Pahangi, Larry A. Mcreynolds, Susan M. Desimone, Steven A. Williams
Cloning And Comparison Of Repeated Dna Sequences From The Human Filarial Parasite Brugia Malayi And The Animal Parasite Brugia Pahangi, Larry A. Mcreynolds, Susan M. Desimone, Steven A. Williams
Biological Sciences: Faculty Publications
No abstract provided.
Metatherian Reproduction: Transitional Or Transcending?, Virginia Hayssen, R. C. Lacy, P. J. Parker
Metatherian Reproduction: Transitional Or Transcending?, Virginia Hayssen, R. C. Lacy, P. J. Parker
Biological Sciences: Faculty Publications
The major difference between metatherian and eutherian reproductive patterns is the relative apportionment of maternal resources to gestation versus lactation, not the total temporal and energetic investment in each reproductive attempt. Gestation accounts for an average of 12% of the time from conception to weaning in marsupials; in placental mammals it accounts for 56% of this period of development. Parental investment at birth is less in marsupials (litter weight averages 0.09% of female body mass) than in eutherians (average 15.5%), but total maternal effort is comparable in the two groups, since the litter weight at weaning is similar for metatherians …
Cloning The Gene For The Malolactic Fermentation Of Wine From Lactobacillus Delbrueckii In Escherichia Coli And Yeasts., Steven A. Williams, Robert A. Hodges, Terry L. Strike, Richard Snow, Ralph E. Kunkee
Cloning The Gene For The Malolactic Fermentation Of Wine From Lactobacillus Delbrueckii In Escherichia Coli And Yeasts., Steven A. Williams, Robert A. Hodges, Terry L. Strike, Richard Snow, Ralph E. Kunkee
Biological Sciences: Faculty Publications
No abstract provided.
Macrophages Contain At Least Two Myosins, John A. Trotter, Stylianos P. Scordilis, Sarkis S. Margossian
Macrophages Contain At Least Two Myosins, John A. Trotter, Stylianos P. Scordilis, Sarkis S. Margossian
Biological Sciences: Faculty Publications
A number of motile functions of macrophages are thought to be mediated by myosin. We have observed that myosin from rabbit alveolar macrophages is heterogeneous with respect to its 20 kDa light chain: two species of 20 kDa light chain are identified by one-dimensional and two-dimensional polyacrylamide gel electrophoresis, in a ratio of 2:1. Native myosin, analyzed on non-denaturing gels, is also composed of two species, in a ratio of 2:1. These results indicate that macrophages contain at least two different myosins, which might have different physiological functions.
Changes In Myosin And Myosin Light Chain Kinase During Myogenesis, Stylianos P. Scordilis, J. Maurice Miles, Robert S. Adelstein, B. William Uhlendorf, Sigfrido Scarpa, Giulio L. Cantoni
Changes In Myosin And Myosin Light Chain Kinase During Myogenesis, Stylianos P. Scordilis, J. Maurice Miles, Robert S. Adelstein, B. William Uhlendorf, Sigfrido Scarpa, Giulio L. Cantoni
Biological Sciences: Faculty Publications
Myosins and myosin light chain kinases have been isolated from a cloned line of myoblasts (L5/A10) as this cell line undergoes differentiation toward adult muscle. At least three myosin isozymes were obtained during this developmental process. Initially a nonmuscle type of myosin was found in the myoblasts. The molecular weights of the myoblast light chains were 20 000 and 15 000. Myosin isolated from early myotubes had light chains with molecular weights of 20 000 and 19 500. Myosin isolated from myotubes which contained sarcomeres had light chains with molecular weights of 23 000, 18 500, and 16000. This last …
A Comparative Study Of The Myosin Light Chain Kinases From Myoblast And Muscle Sources. Studies On The Kinases From Proliferative Rat Myoblasts In Culture, Rat Thigh Muscle, And Rabbit Skeletal Muscle, S. P. Scordilis, R. S. Adelstein
A Comparative Study Of The Myosin Light Chain Kinases From Myoblast And Muscle Sources. Studies On The Kinases From Proliferative Rat Myoblasts In Culture, Rat Thigh Muscle, And Rabbit Skeletal Muscle, S. P. Scordilis, R. S. Adelstein
Biological Sciences: Faculty Publications
Myosin light chain kinases have been isolated from rat thigh and rabbit skeletal muscle and cultured rat myoblasts. From these preparations, two types of kinases can be distinguished: calcium-dependent and calcium-independent. Both types of kinases can phosphorylate isolated P-light chains of myosin from several sources (skeletal muscle, cardiac muscle, and platelet). Data are shown which support the phosphorylation of the same site on the non-muscle P-light chains by both types of kinases. The rates of these reactions are, however, different for the two types of kinases. Kinetic analysis of the myoblast kinase show differing affinities for various P-light chains (non-muscle …
Membrane Potentials And Resistances Of Giant Mitochondria: Metabolic Dependence And The Effects Of Valinomycin, Bruce L. Maloff, Stylianos P. Scordilis, Christopher Reynolds, Henry Tedeschi
Membrane Potentials And Resistances Of Giant Mitochondria: Metabolic Dependence And The Effects Of Valinomycin, Bruce L. Maloff, Stylianos P. Scordilis, Christopher Reynolds, Henry Tedeschi
Biological Sciences: Faculty Publications
The membrane potentials and resistances of giant mitochondria from mice fed cuprizone have been studied. They were found to correspond approx. 10-20 mV, positive inside, and 2 M omega, respectively. These properties were found to be independent of the metabolic state. The microelectrodes were in the inner mitochondrial space since (a) the potentials in the presence of valinomycin depended on the K+ concentration of the medium and magnitude of the K+ diffusion potentials was consistent with the presence of a high internal concentration of K+, (b) almost identical results were obtained with mitochondria from which the external membrane had been …
Assays Of The Metabolic Viability Of Single Giant Mitochondria. Experiments With Intact And Impaled Mitochondria, B. L. Maloff, S. P. Scordilis, H. Tedeschi
Assays Of The Metabolic Viability Of Single Giant Mitochondria. Experiments With Intact And Impaled Mitochondria, B. L. Maloff, S. P. Scordilis, H. Tedeschi
Biological Sciences: Faculty Publications
Single giant mitochondria isolated from mice fed cuprizone were assayed for their metabolic viability. Two tests were devised. One test optically detected the accumulation of calcium phosphate within the mitochondria under massive loading conditions (including the presence of succinate and ATP). The accumulation corresponds to a test of energy coupling from either electron transport or the hydrolysis of ATP since it is blocked by either antimycin A or oligomycin. The other assay tested for the production of ATP from ADP and Pi, using myofibrils. Myofibrils prepared from glycerinated rabbit psoas muscle contract only in the presence of ATP and not …
Characterization Of The Myosin-Phosphorylating System In Normal Murine Astrocytes And Derivative Sv40 Wild-Type And A-Mutant Transformants, S. P. Scordilis, J. L. Anderson, R. Pollack, R. S. Adelstein
Characterization Of The Myosin-Phosphorylating System In Normal Murine Astrocytes And Derivative Sv40 Wild-Type And A-Mutant Transformants, S. P. Scordilis, J. L. Anderson, R. Pollack, R. S. Adelstein
Biological Sciences: Faculty Publications
Myosin and myosin light-chain kinase have been isolated and characterized from small quantities of normal and SV40-transformed, murine astrocytic neuroglial cells in culture and from intact normal mouse brain. Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis of the astrocyte myosins revealed a heavy chain of 200,000 daltons and two light chains of 20,000 and 15,000 daltons. These myosins are similar to other cytoplasmic myosins. The astrocyte 20,000-dalton light chain can be phosphorylated by an endogenous myosin light- chain kinase which has properties similar to those of the myosin light-chain kinase found in human platelets. No differences were detected in either the …
Donnan Potential Of Rabbit Skeletal Muscle Myofibrils I: Electrofluorochromometric Detection Of Potential, S. P. Scordilis, H. Tedeschi, C. Edwards
Donnan Potential Of Rabbit Skeletal Muscle Myofibrils I: Electrofluorochromometric Detection Of Potential, S. P. Scordilis, H. Tedeschi, C. Edwards
Biological Sciences: Faculty Publications
The fluorescence of the dye CC 6 [(3 hexyl 2 (3 hexyl 2 benzoxazolinylidene) 1 propenyl) benzoxazolium iodide] was shown to indicate Donnan potentials in rabbits skeletal muscle myofibrils. These results are in aggreement with previously published work in which the potentials were measured with microelectrodes on glycerol extracted muscle fibers. The magnitude of the Donnan potential of the myofibrils was shown to be dependent on the state (rigor or relaxed) of the system.