Life Sciences Commons^™

Dlp - An Introduction, Denny Vrandecic, Pascal Hitzler, Rudi Studer

Computer Science and Engineering Faculty Publications

DLP - Description Logic Programs - is the name for the common language that is able to integrate knowledge bases described in Description Logic with Logic Programs. In this introduction, we offer a very short overview of DLP, the motivation for it, the benefits it offers and how to use it.

Glyde - An Expressive Xml Standard For The Representation Of Glycan, Satya S. Sahoo, Christopher Thomas, Amit P. Sheth, Cory Andrew Henson, William S. York

Kno.e.sis Publications

The amount of glycomics data being generated is rapidly increasing as a result of improvements in analytical and computational methods. Correlation and analysis of this large, distributed data set requires an extensible and flexible representational standard that is also ‘understood’ by a wide range of software applications. An XML-based data representation standard that faithfully captures essential structural details of a glycan moiety along with additional information (such as data provenance) to aid the interpretation and usage of glycan data, will facilitate the exchange of glycomics data across the scientific community. To meet this need, we introduce GLYcan Data Exchange (GLYDE) …

Semantic Web & Semantic Web Services: Applications In Healthcare And Scientific Research, Amit P. Sheth

Kno.e.sis Publications

No abstract provided.

Discovering Informative Subgraphs In Rdf Graphs, William H. Milnor, Cartic Ramakrishnan, Matthew Perry, Amit P. Sheth, John A. Miller, Krzysztof Kochut

Kno.e.sis Publications

Discovering patterns in graphs has long been an area of interest. In most contemporary approaches to such pattern discovery either quantitative anomalies or frequency of substructure is used to measure the interestingness of a pattern. In this paper we address the issue of discovering informative sub-graphs within RDF graphs. We motivate our work with an example related to Semantic Search. A user might pose a question of the form: ' What are the most relevant ways in which entity X is related to entity Y?' the response to which is a subgraph connecting X to Y. Relevance of the …

Limitations And Potentials Of Current Motif Discovery Algorithms, Jianjun Hu, Bin Li, D. Kihara

Faculty Publications

Computational methods for de novo identification of gene regulation elements, such as transcription factor binding sites, have proved to be useful for deciphering genetic regulatory networks. However, despite the availability of a large number of algorithms, their strengths and weaknesses are not sufficiently understood. Here, we designed a comprehensive set of performance measures and benchmarked five modern sequence-based motif discovery algorithms using large datasets generated from Escherichia coli RegulonDB. Factors that affect the prediction accuracy, scalability and reliability are characterized. It is revealed that the nucleotide and the binding site level accuracy are very low, while the motif level accuracy …

Validation Of A Multiplex Pcr Assay For The Simultaneous Detection Of Human Papillomavirus And Chlamydia Trachomatis In Cervical Preservcyt Samples., Helen Keegan, Alison Malkin, Mairead Griffin, Fergus Ryan, Helen Lambkin

Articles

No abstract provided.

Real-Time Reverse Transcription Pcr For Detection And Quantitative Analysis Of Equine Influenza Virus., Michelle Quinlivan, Eugene Dempsey, Fergus Ryan, Sean Arkins, Ann Cullinnane

Articles

Equine influenza is a cause of epizootic respiratory disease of the equine. The detection of equine influenza virus using real-time Light Cycler reverse transcription (RT)-PCR technology was evaluated over two influenza seasons with the analysis of 171 samples submitted for viral respiratory disease. Increased sensitivity was found in overall viral detection with this system compared to Directigen Flu A and virus isolation, which were 40% and 23%, respectively, that of the RT-PCR. The assay was also evaluated as a viable replacement for the more traditional methods of quantifying equine influenza virus, 50% egg infectious dose and 50% tissue culture infectious …

A Web Services Architecture For Umls Knowledge Sources, Parsa Mirhaji, Narendra Kunapareddy, Yanko Michea, Arunkumar Srinivasan

Faculty, Staff and Student Publications

A web service is a collection of industry standards to enable reusability of services and interoperability of heterogeneous applications. The UMLS Knowledge Source (UMLSKS) Server provides remote access to the UMLSKS and related resources. We propose a Web Services Architecture that encapsulates UMLSKS-API and makes it available in distributed and heterogeneous environments. This is the first step towards intelligent and automatic UMLS services discovery and invocation by computer systems in distributed environments such as web.

Using Incomplete Citation Data For Medline Results Ranking, Jorge R Herskovic, Elmer V Bernstam

Faculty, Staff and Student Publications

Information overload is a significant problem for modern medicine. Searching MEDLINE for common topics often retrieves more relevant documents than users can review. Therefore, we must identify documents that are not only relevant, but also important. Our system ranks articles using citation counts and the PageRank algorithm, incorporating data from the Science Citation Index. However, citation data is usually incomplete. Therefore, we explore the relationship between the quantity of citation information available to the system and the quality of the result ranking. Specifically, we test the ability of citation count and PageRank to identify "important articles" as defined by experts …

Persuasive Email Messages For Patient Communication, Muhammad Walji, Kathy Johnson-Throop, Todd Johnson, Elmer Bernstam, Jiajie Zhang

Faculty, Staff and Student Publications

To improve health and reduce costs, we need to encourage patients to make better health care decisions. Since email is widely available, it may be useful for patient-directed interventions. However, we know little about how the contents of an email message can influence a health-related decision. We propose a model to understand how patients may process persuasive email messages.

Interruptions In Workflow For Rns In A Level One Trauma Center, Juliana J. Brixey, David J. Robinson, Zhihua Tang, Todd R. Johnson, Jiajie Zhang, James P. Turley

Faculty, Staff and Student Publications

An understanding of interruptions in healthcare is important for the design, implementation, and evaluation of health information systems and for the management of clinical workflow and medical errors. The purpose of this study is to identify and classify the types of interruptions experienced by Emergency Department(ED) nurses working in a Level One Trauma Center. This was an observational field study of Registered Nurses (RNs) employed in a Level One Trauma Center using the shadowing method. Results of the study indicate that nurses were both recipients and initiators of interruptions. Telephones, pagers, and face-to-face conversations were the most common sources of …

Using Technology To Teach Technology: Design And Evaluation Of Bilingual Online Physician Education About Electronic Medical Records, Sarah R. Edmonson, Adol Esquivel, Pallavi Mokkarala, Craig W. Johnson, Cynthia L. Phelps

Faculty, Staff and Student Publications

The "EMR Tutorial" is designed to be a bilingual online physician education environment about electronic medical records. After iterative assessment and redesign, the tutorial was tested in two groups: U.S. physicians and Mexican medical students. Split-plot ANOVA revealed significantly different pre-test scores in the two groups, significant cognitive gains for the two groups overall, and no significant difference in the gains made by the two groups. Users rated the module positively on a satisfaction questionnaire.

Predicting Childhood Sexual Or Physical Abuse: A Logistic Regression Geo-Mapping Approach To Prevention, Roland K. Tadoum, Kamila Smolij, Michelle A. Lyn, Craig W. Johnson

Faculty, Staff and Student Publications

This study investigates the degree to which gender, ethnicity, relationship to perpetrator, and geomapped socio-economic factors significantly predict the incidence of childhood sexual abuse, physical abuse and non- abuse. These variables are then linked to geographic identifiers using geographic information system (GIS) technology to develop a geo-mapping framework for child sexual and physical abuse prevention.

Regulatory Role Of Glycogen Synthase Kinase 3 For Transcriptional Activity Of Add1/Srebp1c, Kang Ho Kim, Min Jeong Song, Eung Jae Yoo, Sung Sik Choe, Sang Dai Park, Jae Bum Kim

Faculty, Staff and Student Publications

Adipocyte determination- and differentiation-dependent factor 1 (ADD1) plays important roles in lipid metabolism and insulin-dependent gene expression. Because insulin stimulates carbohydrate and lipid synthesis, it would be important to decipher how the transcriptional activity of ADD1/SREBP1c is regulated in the insulin signaling pathway. In this study, we demonstrated that glycogen synthase kinase (GSK)-3 negatively regulates the transcriptional activity of ADD1/SREBP1c. GSK3 inhibitors enhanced a transcriptional activity of ADD1/SREBP1c and expression of ADD1/SREBP1c target genes including fatty acid synthase (FAS), acetyl-CoA carboxylase 1 (ACC1), and steroyl-CoA desaturase 1 (SCD1) in adipocytes and hepatocytes. In contrast, overexpression of GSK3beta down-regulated the transcriptional …

Incremental Genetic K-Means Algorithm And Its Application In Gene Expression Data Analysis, Yi Lu, Shiyong Lu, Farhad Fotouhi, Youping Deng, Susan J. Brown

Faculty Publications

Background

In recent years, clustering algorithms have been effectively applied in molecular biology for gene expression data analysis. With the help of clustering algorithms such as K-means, hierarchical clustering, SOM, etc, genes are partitioned into groups based on the similarity between their expression profiles. In this way, functionally related genes are identified. As the amount of laboratory data in molecular biology grows exponentially each year due to advanced technologies such as Microarray, new efficient and effective methods for clustering must be developed to process this growing amount of biological data.

Results

In this paper, we propose a new clustering algorithm, …

Finding Cancer Subtypes In Microarray Data Using Random Projections, Debashis Ghosh

The University of Michigan Department of Biostatistics Working Paper Series

One of the benefits of profiling of cancer samples using microarrays is the generation of molecular fingerprints that will define subtypes of disease. Such subgroups have typically been found in microarray data using hierarchical clustering. A major problem in interpretation of the output is determining the number of clusters. We approach the problem of determining disease subtypes using mixture models. A novel estimation procedure of the parameters in the mixture model is developed based on a combination of random projections and the expectation-maximization algorithm. Because the approach is probabilistic, our approach provides a measure for the number of true clusters …

Semantic Web Technology In Support Of Bioinformatics For Glycan Expression, Amit P. Sheth, William S. York, Christopher Thomas, Meenakshi Nagarajan, John A. Miller, Krzysztof Kochut, Satya S. Sahoo, Xiaochuan Yi

Kno.e.sis Publications

Due to the complexity of biological systems, interpretation of data obtained by a single experimental approach can often be interpreted only if viewed from a broader context, taking into account the information obtained by many diverse techniques. The vast amount of interpreted experimental data that is now available via the internet opens the possibility of collecting the relevant pieces of information that will enable scientists to form hypotheses based on the integration of this diverse information. However, the sheer volume of data that is available makes it very difficult to select the information necessary to make a coherent model of …

Lsdis: Large Scale Distributed Information Systems Lab, Amit P. Sheth

Kno.e.sis Publications

The LSDIS (Large Scale Distributed Information Systems) lab was established in 1994 with the guidance and direction provided by Dr. Amit P. Sheth with the help of Dr. John A. Miller and Dr. Krzysztof J. Kochut. In 1998 this faculty group was further strengthened by the addition of Dr. Ismailcem B. Arpinar. LSDIS is the largest research group in Computer Science at UGA and one of the strongest in its area. During Fall 2004, it is funding 15 students (majority of them PhD), and has one research staff.

Over the years LSDIS has been actively involved in research projects in …

Logic Programs, Iterated Function Systems, And Recurrent Radial Basis Function Networks, Sebastian Bader, Pascal Hitzler

Computer Science and Engineering Faculty Publications

Graphs of the single-step operator for first-order logic programs—displayed in the real plane—exhibit self-similar structures known from topological dynamics, i.e., they appear to be fractals, or more precisely, attractors of iterated function systems. We show that this observation can be made mathematically precise. In particular, we give conditions which ensure that those graphs coincide with attractors of suitably chosen iterated function systems, and conditions which allow the approximation of such graphs by iterated function systems or by fractal interpolation. Since iterated function systems can easily be encoded using recurrent radial basis function networks, we eventually obtain connectionist systems which …

Flash Artifact Suppression In Two-Dimensional Ultrasound Imaging, Richard Yung Chiao, Gregory Ray Bashford, Mark Peter Feilen, Cynthia Andrews Owen

Biomedical Imaging and Biosignal Analysis Laboratory

Flash artifacts in ultrasound flow images are suppressed to achieve enhanced flow discrimination. Flash artifacts typically occur as region of elevated signal strength (brightness or equivalent color) within an image. A flash suppression algorithm included the steps of estimating the flash within an image and then suppressing the estimated flash. The mechanism for flash suppression is spatial filtering. An extension of this basic method used information from adjacent frames to estimate the flash and/or to smooth the resulting image sequence. Temporal information from adjacent frames is used as an adjunct to improve performance.

Enhancing Web Services Description And Discovery To Facilitate Composition, Preeda Rajasekaran, John A. Miller, Kunal Verma, Amit P. Sheth

Kno.e.sis Publications

Web services are in the midst of making the transition from being a promising technology to being widely used in the industry. However, most efforts to use Web services have been manual, thus slowing down the ever changing and dynamic businesses of today. In this paper, we contend that more expressive descriptions of Web services will lead to greater automation and thus provide more agility to businesses. We present the METEOR-S front-end tools for source code annotation and semantic Web service description generation. We also present WSDL-S, a language created for incorporating semantic descriptions in the industry wide accepted WSDL, …

Workflow Management Systems And Erp Systems: Differences, Commonalities, And Applications, Jorge Cardoso, Robert P. Bostrom, Amit P. Sheth

Kno.e.sis Publications

Two important classes of information systems, Workflow Management Systems(WfMSs) and Enterprise Resource Planning (ERP) systems, have been used to support e-business process redesign, integration, and management. While both technologies can help with business process automation, data transfer, and information sharing, the technological approach and features of solutions provided by WfMS and ERP are different. Currently, there is a lack of understanding of these two classes of information systems in the industry and academia, thus hindering their effective applications. In this paper, we present a comprehensive comparison between these two classes of systems. We discuss how the two types of systems …

Learning Mixture Models With The Regularized Latent Maximum Entropy Principle, Shaojun Wang, Dale Schuurmans, Fuchun Peng, Yunxin Zhao

Kno.e.sis Publications

This paper presents a new approach to estimating mixture models based on a recent inference principle we have proposed: the latent maximum entropy principle (LME). LME is different from Jaynes' maximum entropy principle, standard maximum likelihood, and maximum a posteriori probability estimation. We demonstrate the LME principle by deriving new algorithms for mixture model estimation, and show how robust new variants of the expectation maximization (EM) algorithm can be developed. We show that a regularized version of LME (RLME), is effective at estimating mixture models. It generally yields better results than plain LME, which in turn is often better than …

Discovery Of Web Services In A Federated Registry Environment, Kaarthik Sivashanmugam, Kunal Verma, Amit P. Sheth

Kno.e.sis Publications

The potential of a large scale growth of private and semi-private registries is creating the need for an infrastructure which can support discovery and publication over a group of autonomous registries. Recent versions of UDDI have made changes to accommodate interactions between distributed registries. In this paper, we discuss METEOR-S Web service Discovery Infrastructure, which provides an ontology-based infrastructure to access a group of registries that are divided based on business domains and grouped into federations. We also discuss how Web service discovery is carried out within a federation.

Efficacy Of Quality Criteria To Identify Potentially Harmful Information: A Cross-Sectional Survey Of Complementary And Alternative Medicine Web Sites, Muhammad Walji, Smitha Sagaram, Deepak Sagaram, Funda Meric-Bernstam, Craig Johnson, Nadeem Q Mirza, Elmer V Bernstam

Faculty, Staff and Student Publications

BACKGROUND: Many users search the Internet for answers to health questions. Complementary and alternative medicine (CAM) is a particularly common search topic. Because many CAM therapies do not require a clinician's prescription, false or misleading CAM information may be more dangerous than information about traditional therapies. Many quality criteria have been suggested to filter out potentially harmful online health information. However, assessing the accuracy of CAM information is uniquely challenging since CAM is generally not supported by conventional literature. OBJECTIVE: The purpose of this study is to determine whether domain-independent technical quality criteria can identify potentially harmful online CAM content. …

Differential Expression With The Bioconductor Project, Anja Von Heydebreck, Wolfgang Huber, Robert Gentleman

Bioconductor Project Working Papers

A basic, yet challenging task in the analysis of microarray gene expression data is the identification of changes in gene expression that are associated with particular biological conditions. We discuss different approaches to this task and illustrate how they can be applied using software from the Bioconductor Project. A central problem is the high dimensionality of gene expression space, which prohibits a comprehensive statistical analysis without focusing on particular aspects of the joint distribution of the genes expression levels. Possible strategies are to do univariate gene-by-gene analysis, and to perform data-driven nonspecific filtering of genes before the actual statistical analysis. …

Sweto: Large-Scale Semantic Web Test-Bed, Boanerges Aleman-Meza, Chris Halaschek, Amit P. Sheth, I. Budak Arpinar, Gowtham Sannapareddy

Kno.e.sis Publications

The emergent Semantic Web community needs a common infrastructure for testing the scalability and quality of new techniques and software which use machine processable data. Since ontologies are a centerpiece of most approaches, we believe that for an accurate evaluation of tools for quality, scalability and performance, the research community needs a freely available ontology with a large description base. If the use of tools is to be for advanced semantic applications, such as those in business intelligence and national security, then instances in the knowledge base should be highly interconnected. Thus, we propose and describe a Semantic WEb Technology …

Statistical Analyses And Reproducible Research, Robert Gentleman, Duncan Temple Lang

Bioconductor Project Working Papers

For various reasons, it is important, if not essential, to integrate the computations and code used in data analyses, methodological descriptions, simulations, etc. with the documents that describe and rely on them. This integration allows readers to both verify and adapt the statements in the documents. Authors can easily reproduce them in the future, and they can present the document's contents in a different medium, e.g. with interactive controls. This paper describes a software framework for authoring and distributing these integrated, dynamic documents that contain text, code, data, and any auxiliary content needed to recreate the computations. The documents are …

A Model Based Background Adjustment For Oligonucleotide Expression Arrays, Zhijin Wu, Rafael A. Irizarry, Robert Gentleman, Francisco Martinez Murillo, Forrest Spencer

Johns Hopkins University, Dept. of Biostatistics Working Papers

High density oligonucleotide expression arrays are widely used in many areas of biomedical research. Affymetrix GeneChip arrays are the most popular. In the Affymetrix system, a fair amount of further pre-processing and data reduction occurs following the image processing step. Statistical procedures developed by academic groups have been successful at improving the default algorithms provided by the Affymetrix system. In this paper we present a solution to one of the pre-processing steps, background adjustment, based on a formal statistical framework. Our solution greatly improves the performance of the technology in various practical applications.

Affymetrix GeneChip arrays use short oligonucleotides to …

Semantic Web Technology Evaluation Ontology (Sweto): A Test Bed For Evaluating Tools And Benchmarking Applications, Boanerges Aleman-Meza, Amit P. Sheth, I. Budak Arpinar, Chris Halaschek

Kno.e.sis Publications

No abstract provided.