Life Sciences Commons^™

Synthesis Of Phosphatidyl Peptides I. O-(Distearoyl-L-A-Glycerylphosphoryl)-L-Serylglycylglycine / By Erich Baer, Jonas Maurukas, And Donald D. Clarke (From The Banting And Best Department Of Medical Research, University Of Toronto, Toronto, Canada), Erich Baer, Jonas Maurukas, Donald Dudley Clarke Phd

Chemistry Faculty Publications

The synthesis of a phosphatidyl tripeptide has been accomplished. It was prepared by (a) phosphorylation of D-α,β-distearin with phenylphosphoryl dichloride and pyridine, (b) esterification of the resulting distearoylL- α-glycerylphenylphosphoryl chloride with N-carbobenzoxy-L-serylglycylglycine benzyl ester in the presence of lutidine, and (c) removal of the protective groups by catalytic hydrogenolysis. The O-( distearoyl-L-α-glycerylphosphoryl)- L-serylglycylglycine was obtained in an over-all yield of 12 per cent. The synthesis of N-carbobenzoxy-L-serylglycylglycine benzyl ester, for which two procedures have been reported, has been simplified considerably by condensing N -carbobenzoxy-L-serine with glycylglycine benzyl ester by means of N ,N' -dicyclohexylcarbodiimide. The phosphatidyl tripeptide is cleaved by …

Metabolic Activity Of Protein Amide Groups, Donald Dudley Clarke Phd, Amos Neidle, Nirmal K. Sarkar, Heinrich Waelsch

Chemistry Faculty Publications

No abstract provided.

Oxidation Of Molybdenite With The Aid Of Microorganisms, Ralph Anderson

Theses and Dissertations

This work represents a study on the biological oxidation of molybdenite, MoS2. The principal objective of this study was to investigate the possibility of the biological oxidation of molybdenite, to determine the physical and chemical conditions under which the oxidative process occurs, and to develop a strain of bacteria or alter the activity of the microorganisms by acclimatization to increase their activity on sulfide minerals, in particular molybdenite. Minerals used in this study were pyrite (FeS2), chalcopyrite (CuFeS2), molybdenite concentrate, and molybdenite ore. The microorganisms used in this investigation were autotrophic bacteria obtained from the leaching streams of Bingham Canyon, …

Photo-Oxidations Sensitized By Physiologically Important Substances, Ernel D. Ihnen

Theses and Dissertations

A study of the photo-oxidation of the 2,6-dichlorophenol indophenol-ascorbic couple was undertaken with a view to investigating the postulated mechanism of oxidation by a component of the oxygen evolving sequence operative in photosynthesis. A spectrophotometric method for the measurement of the progress of photo-oxidation was developed. Temperature and pH dependence were determined and the effect of various common inhibitors and physiological substances tested. None of the common Hill reaction inhibitors were effective in the ascorbic acid photo-oxidation in the system used. KCN and catalase as well as a few other high molecular weight proteins were able to inhibit dark auto-oxidation …

L-Serylglycylglycine / By Erich Baer, Jonas Maurukas, And Donald D. Clarke, Erich Baer, Jonas Maurukas, Donald Dudley Clarke Phd

Chemistry Faculty Publications

A synthesis of the hitherto unreported L-serylglycylglycine by two different methods is described. N-carbobenzoxy L-serylglycylglycine benzyl ester, an intermediate in the preparation of the tripeptide, was found to be a suitable derivative in the synthesis of L-α-phosphatidyl L-serylglycylglycine

Radicinin: A Metabolite From Stemphylium Radicinum. I. Chemistry And Action / D. D. Clarke And F. F. Nord From The Department Of Organic Chemistry And Enzymology, Fordham University, New York, New York, Donald Dudley Clarke Phd, Friedrich F. Nord

Chemistry Faculty Publications

The conditions for the growth of Sterad and isolation of radicinin are described. The formula C12H1205 wasas established for this compound by preparation and analyses of several derivatives. Degradation and spectrophotometric studies were employed to establish the molecular formula. Studies on the mode of action of radicinin revealed that it caused an increase in the rate of dehydrogenation of isopropyl alcohol by FIB, but that it had no effect on the quantity or degree of desaturation of the fat synthesized by FIB

Radicinin: A Metabolite From Stemphylium Radicinum. Ii. On The Mechanism Of Its Biosynthesis / D. D. Clarke And F. F. Nord From The Department Of Organic Chemistry And Enzymology, Fordham University, New York, New York, Donald Dudley Clarke Phd, Friedrich F. Nord

Chemistry Faculty Publications

After studying the effect of various substrates and also of certain enzyme inhibitors on the rate of formation of radicinin by Sterad, it is suggested that the carbohydrate molecule is utilized intact in the biosynthesis of radicinin

Ultraviolet Absorption Spectra Of Derivatives Of 2,3,5- And 2,4,5-Trihydroxyacetophenone / By D. D. Clarke And F. F. Nord, Donald Dudley Clarke Phd, Friedrich F. Nord

Chemistry Faculty Publications

No abstract provided.

The Chromatographic Purification Of Human Red Blood Cell Acetylcholinesterase, Richard P. Dunkley

Theses and Dissertations

A process of column chromatography of human erythrocyte acetylcholinesterase, using a cationic exchange resin, was investigated as a possible method for the primary partial purification of the enzyme on a large scale. The investigation was based, first; on a detailed study of the enzymatic stability to variation in hydrogen-ion and salt concentrations for periods of time extending to forty eight hours and, second; on an investigation of the degree of adsorption by the resin of the enzyme at various pH values within the pH range of enzyme stability. A method for the analysis of the enzyme activity of large numbers …

Biological Oxidation Of Copper Sulfide Minerals, Delmar Boyd Davis

Theses and Dissertations

This work represents a study of the biological oxidation of copper sulfide minerals. The principal objective of the study was to determine the extent of bacterial oxidation on copper containing sulfide minerals. It was also desired to determine if the bacteria could oxidize copper sulfides in the absence of iron. A better understanding of the nitrogen requirements of the organisms was desired. Minerals used in this study were Bingham Canyon Float Concentrate, chalcopyrite, covellite, calcocite, bornite, tetrahedrite, and reagent grade copper sulfide. The bacteria used were obtained from the mine waters of Bingham Canyon, Utah. To study the problem the …

Radicinin: A New Pigment From Stemphylium Radicinum / D. D. Clarke F. F. Nord Department Of Organic Chemistry And Enzymology, Fordham University, New York 58, New York, Donald Dudley Clarke Phd, Friedrich F. Nord

Chemistry Faculty Publications

No abstract provided.

Concerning The Site Of Nitrogen Absorption In Rats Fed Autoclaved Or Raw Soybean Oil Mea, Raymond Borchers

Department of Biochemistry: Faculty Publications

Carroll, Hensley, and Graham (1) have concluded that much of the nitrogen absorption in rats fed raw soybean oil meal must take place in the cecum. This conclusion was reached from data showing that the apparent digestibility of raw soybean nitrogen in the terminal 20% of the small intestine was 32.65%, whereas in the feces the value was 76.96%. Values reported, for apparent digestibility of heated soybean nitrogen were 78.66% and 81.78%, respectively. This observation presented a notable advance in explaining the lower nutritive value of raw soybeans compared with autoclaved soybeans. It therefore seemed advisable to repeat this work …

A Metabolic Relationship Between The Aromatic Amino Acids, Joseph F. Nyc, Francis A. Haskins, Herschel K. Mitchell

Department of Agronomy and Horticulture: Faculty Publications

The present work involves a further investigation of a Neurospora mutant, C-86, previously mentioned by Lein, Mitchell and Houlahan (1) as one that can utilize anthranilic acid, indole, tryptophan, kynurenine, 3-hydroxyanthranilic acid, and nicotinic acid as supplements for growth. Mutant C-86, when crossed to a “wild-type” strain, was found to differ from this wild type strain, with respect to tryptophan biosynthesis, by a mutation at a single locus. The evidence presented suggests the possibility of a common precursor to the aromatic amino acids.

Pathological Physiology As A Basis For Medical Practice, Walter E. Macpherson

Medical Arts and Sciences: A Scientific Journal of the College of Medical Evangelists

No abstract provided.

Rationalists In Retreat, W. E. Filmer

Stone-Campbell Books

No abstract provided.

Recent Opinions Of Biologists On Evolution: 2nd Edition, Evolution Protest Movement

Stone-Campbell Books

No abstract provided.

Methemoglobin., Herbert Friedman

Electronic Theses and Dissertations

No abstract provided.

Cerebral Anoxia And Its Residuals: Pt. Iii. The Structural Changes, Cyril B. Courville

Medical Arts and Sciences: A Scientific Journal of the College of Medical Evangelists

No abstract provided.

The Circulatory Effects Of Histamine, Fred B. Moor

Medical Arts and Sciences: A Scientific Journal of the College of Medical Evangelists

No abstract provided.

Riboflavin Production By Molds, George L. Peltier, Raymond Borchers

Department of Biochemistry: Faculty Publications

Very little information is at hand regarding the ability of molds to synthesize riboflavin. Few citations pertaining directly to riboflavin production by true

molds are available. Pontovich (1943) found as much as 2 mg riboflavin per g

of Aspergillus flavus mycelium. Tanner et at. (1945) determined the quantity of

riboflavin in the submerged fermentation media of Penicillium chrysogenum.

The highest value found was 1. 36 mg per ml. The primary purpose of this study

was to screen several hundred isolates, recently obtained from soil, crop residues,

and composts, for their ability to produce riboflavin on a wheat bran substrate.

Studies On Synthesis By Enzymatic Catalysis., Charles Henry Wabnitz 1916-2004

Electronic Theses and Dissertations

No abstract provided.

The Effect Of Adrenaline Injection On Glycogenolysis And Glycogenesis In Rat Livers, Virginia Mary Zingsheim

Bachelors’ Theses

Present day knowledge concerning the liver is derived from many sources and the accumulation of many methods of investigation. Sugar, which is needed by the body for energy, is stored in the liver as glycogen. Claude Bernard (26), an eminent physiologist, was the first to suspect that carbohydrates were stored in the liver. Faulty glycogen synthesis and hydrolysis results in one of our most widespread diseases, diabetes. Thus, many biochemists and physiologists have tried to find the mechanism of the process, and the primary and secondary facts which affect glycogenesis and glycogenolysis. Although the chemical secretions and hormones of such …

A Study Of Factors Influencing The Hydrolysis Of Corn Amylopectin By Barley Malt Enzymes., Thelma Munsey Back 1918-2006

Electronic Theses and Dissertations

No abstract provided.

The Study Of The Effects Of Paradichlorbenzene On Drosophila Melanogaster, Dwain J. Paal

Bachelors’ Theses

The object of this investigation was to produce polyploid cells in the salivary glands of Drosophila melanogaster by treatment with paradichlorbenzene. Polyploidy is the condition in which the number of chromosomes in the nucleus is an exact multiple of the somatic or diploid number greater than two. This phenomena has often been referred to as produced by incomplete nuclear division.

Threonine Deficiency In Hydrolysates Of Zein Prepared By Autoclaving, Raymond Borchers, John R. Totter, Clarence P. Berg

Department of Biochemistry: Faculty Publications

In a previous paper (Borchers and Berg, 1942) we showed that

autoclaving zein with sulfuric acid longer than necessary for complete

hydrolysis causes destruction and racemization; either of

these might account for the failure of such a hydrolysate to

promote growth in young rats when substituted for a hvdrolysate,

prepared by refiuxing, in a diet which produced moderate growth.

It seemed to us that this deterioration in the dietary protein might

well be the result primarily of essential amino acid deficiencies

which could be detected and overcome by appropriate supplementation.

Because threonine is known to be present in zein in …

The Effect Of Conditions Of Hydrolysis And Of Prolonged Heating Upon The Optical Rotation Of Sulfuric Acid Hydrolysates Of Zein, Raymond Borchers, Clarence P. Berg

Department of Biochemistry: Faculty Publications

Some time ago we observed that a sulfuric acid hydrolysate of zein prepared in an autoclave at 165˚ did not support growth in young rats when it was supplemented with certain amino acids, even though the same amino acids, added to a hydrolysate prepared by refluxing, permitted moderate growth. Since the optical rotation of the autoclaved hydrolysate was lower than that of the refluxed, either destruction or racemization of essential constituents, or both, might have occurred. Loss of amino nitrogen and production of extra ammonia upon prolonged autoclaving of proteins with acids have been noted by Van Slyke (1912) …

Tryptophane Metabolism X. The Effect Of Feeding 1(-)-, Dl-, And D(+)-Tryptophane, D(-)And Dl-(Β-3-Indolelactic Acid,(Β-3-Indolepyruvic Acid, And L(-)Kynurenine Upon The Storage Of Liver Glycogen And The Urinary Output Of Kynurenic Acid, Kynurenine, And Total Acetone Bodies, Raymond Borchers, Clarence P. Berg, Newton E. Whitman

Department of Biochemistry: Faculty Publications

The amount of tryptophane required for maintenance and growth is small (2). When an excess is supplied to some animals, kynurenic acid is excreted (3) and, under certain conditions, kynurenine also (4). Although these are quantitatively the most important of the known metabolic products of tryptophane, the amounts excreted in extensive tests in the dog and rabbit have usually accounted for less than half, more often for less than a third, of the tryptophane administered (4-6).

Activated Carbon From Grapefruit Waste., Delbert Van Fletcher 1918-2005

Electronic Theses and Dissertations

No abstract provided.

The Effects Of An Insufficient Carbohydrate Diet On The Leucocytes Of The Normal Albino Rat, Carl Frederick Glienke

Bachelors’ Theses

This thesis deals with the effects of an insufficient carbohydrate diet on the differential counts of the leucocytes of the common albino rat. The experiment was chosen because of the interesting problem it presented and because little could be found on work done regarding it. To the ’writer’s knowledge, very little work has been done in even establishing a definite differential count of a normal rat, much less working on one under an abnormal condition. Because of this lack of information, a normal count was first established for the strain used, and then this count was compared with that of …

A Study Of The Kjeldahl Method And A Modification For The Determination Of Nitrogen In Alcoholic Distillates., Margie M. Miller

Electronic Theses and Dissertations

No abstract provided.