Life Sciences Commons^™

The Biochemical Response Of Provo Bay To Nutrient Inflow, R. Bruce Sundrud

Theses and Dissertations

Provo Bay of Utah Lake, Utah, receives the effluents from farms, industry, and three cities. In order to determine the effects of these effluents, eleven stations were established throughout the Bay. At weekly intervals from June 19 to October 26, 1970, and monthly thereafter until March, 1971, the water at these stations was sampled for dissolved oxygen (DO}, carbon dioxide (CO2), turbidity, pH, phosphates, nitrates, biochemical oxygen demand (BOD) and coliform bacteria. Due to intense algal blooms, the quality of the water changes as it passes through Provo Bay. Average values for the inflow, mid-Bay, and point of discharge respectively …

Effects Of Insulin, Sodium And D-Glucose On Amino Acid Absorption In The Intestine Of Rats, Andre-Gerard Craan

Dissertations and Theses

Amino acid absorption across rat intestine in vitro was inhibited or stimulated by insulin depending on the hormone serosal concentration.

The absence of sodium ions from the incubating solution resulted in a significant decrease of L-alanine absorption. However, L-alanine was enhanced in a sodium-free medium by the addition of insulin on the serosal side of the intestine.

A sizable decrease in L-alanine and L-lysine absorption was produced by introduction of D-glucose (0.2 % and 2 %) in the Krebs-Ringer's incubating buffer. Nevertheless, the presence of D-glucose did not prevent the stimulatory effect of insulin on amino acid absorption.

The Regulatory Effects Of Certain S-Triazine Compounds On The Nutritive Composition Of Seeds And Leaves Of The Pea (Pisum Sativum L.) And Sweet Corn (Zea Mays L.) And Responses Of Metabolic Systems, Ming-Tsung Wu

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The study was conducted to investigate the influence of eight s-triazine compounds--simazine, atrazine, terbutryne, GS-14254, propazine, prometryne, prometone, and ametryne--on the composition of seeds of peas and sweet corn through soil and foliar application. In order to understand the mechanism of the effects of these a-triazines on protein accumulation, the responses of the metabolic systems of pea and sweet corn seedlings to s-triazine treatment was studied with emphasis on the stimulation of certain enzymes, and the changes in the rates of incorporation of ¹⁴C-L-leucine (U) into soluble protein. In addition, the effect of simazine on the ultrastructure …

A Conceptual Draft Of A Dynamic Hydro-Biological Model For Lake Mead, L. G. Everett, Bureau Of Reclamation

Publications (WR)

Food and energy transformation in an aquatic system must be understood in order to develop a simulation approach. This report provides a comprehensive study of an aquatic ecosystem. The objectives of this report are to show: (1) the biological relationships in an aquatic system (2) the role of nutrients in the biological cycle (3) the role of abiotic factors in a limnetic environment (4) the status of the art of "Eutrophication modeling".

Triplet-Triplet Energy Transfer In Enzyme-Inhibitor Complexes, Ronald Earl Hauxwell

Masters Theses

No abstract provided.

A Study Of Glycosphingolipids In Aorta, Blood And Spleen And The Effects Of Atherogenic Diets On These Lipids In Rabbits, Eric Coles

Dissertations

No abstract provided.

Ultraviolet-Stimulated Thymidine Incorporation In Xeroderma Pigmentosum Lymphocytes / Peter G. Burk Marvin A. Lutzner Donald D. Clarke And Jay H. Robbins Bethesda, Md And Bronx, N.Y. From The Dermatology Branch, National Cancer Institute, National Institutes Of Health And The Chemistry Department, Fordham University, Peter G. Burk, Marvin A. Lutzner, Donald Dudley Clarke Phd, Jay H. Robbins

Chemistry Faculty Publications

Autoradiographic and liquid scintillation counting techniques were utilized to measure the ultraviolet (UV)-stimulated incorporation of radioactive thymidine during repair replication of the UV-damaged deoxyribonucleic acid (DNA) of irradiated peripheral blood lymphocytes from 4 patients w ith xeroderma pigmentosum (XDP) and from normal donors. Lymphocytes from 3 of the 4 patients had a markedly decreased rate of incorporation soon after irradiation in comparison with the rate obtained with normal donors' lymphocytes. However, the duration of incorporation into these 3 patients' lymphocytes was prolonged, so that XDP lymphocytes appear eventually to incorporate as much total thymidine as normal lymphocytes. Lymphocytes from the …

Rapid Electroosmosis Measurements, James O. Wear

Journal of the Arkansas Academy of Science

A cell has been designed and built that allows for rapid measurement of volume moved in a definite time by electroosmosis. The cell is simple to use and is not very elaborate. Using a water jacket, the cell temperature can be controlled to ± 0.1° C. Measurements are presented for acetonitrile, dimethylformamide, and nitrobenzene at 25° C for applied voltages of 25, 50, 75, and 100 volts.

Micronutrients And Biological Patterns In Lake Mead, Hasan K. Qashu, Lorne G. Everett, J. S. Carlson, Bureau Of Reclamation

Publications (WR)

Progressive increases in concentration of dissolved solids in the Colorado River water from Lake Powell to Imperial Dam seem to alter plankton dynamics and biological productivity of the river. Also, changes in biological productivity and micronutrients concentrations occur within the same reservoir. Development of a digital simulation model to predict micronutrients concentrations and biological productivity is necessary for diagnosing changes in plankton population and effluent-carrying capacity of the system. The objectives of the study are: (1) to determine trace metal balance at different locations in Lake Mead, (2) to measure biological productivity and conduct plankton population counts at each sampling …

The Nature Of Salmonella Phage P3 Modification And Restriction By Growth In Escherichia Coli, Clark A. Pearson

Loma Linda University Electronic Theses, Dissertations & Projects

The Salmonella potsdam group B bacteriophage P3, which grows in the rough variant strain of S. potsdam (SP2-R) and E. coli K_CS11, K_W117, and C⁺/L with similar efficiencies, was restricted by SP2-R after a passage though E. coli. The entrance of restricted E. coli-grown phage DNA into nonpermissive SP2-R cells was confirmed by the finding that after adsorption of restricted ³²P-labeled P3-C₁·C⁺/L to SP2-R and removal of the adsorbed phage heads from cells, 80.3% of the labeled DNA activity sedimented with the cells. Thus restricted P3 DNA …

High-Pressure Phase Studies On Sodium–Potassium Alloys, D. Ray Anderson, J. Bevan Ott, Rex J. Goates, H. Tracy Hall

Faculty Publications

The solid–liquid phase diagrams were investigated to 60 kbar for elemental sodium, potassium, and various sodium–potassium alloys. The data for Na and K are in general agreement with those of earlier workers. In the alloy system the temperatures of both the peritectic and eutectic halts increase as the pressure increases, but with different slopes, so that the two invariant points merge at approximately 35 kbar. Above this pressure, two halts are again observed. One increases with increasing pressure, and the other decreases with increasing pressure up to 55 kbar, where it abruptly starts increasing again. Melting points for samples in …

A Comparison Of Luteinizing Hormone And Prostaglandin F2 Α Stimulation Of Pseudo-Pregnant Rat Ovarian Steroidogenesis In Vitro, Ross R. Erickson

Masters Theses

No abstract provided.

Purification And Properties Of Dna Polymerase From Bacillus Licheniformis And Bacillus Stearothermophilus, Bruce A. Roe

Dissertations

No abstract provided.

The Effects Of 2-Chloro-4-Ethylamino-6-Isopropylamino-S-Triazine (Atrazine) On The Activities Of Certain Calvin Cycle Enzymes In Barley And Corn, L. Chris Harrison

Theses and Dissertations

Atrazine (2-chloro-4, ethylamino-6-isopropylaminos- triazine) applied to Hordium vulgare L. variety barbless in low concentrations (0.001, 0.01, 0.1 uM) was shown to in--­ crease the activity of D-ribulose-1, 5-diphosphate carboxylase (RuDPCase) and phosphoribulokinase (PRuK) up to 23% and 30% respectively and increase the amount of chlorophyll and extractable protein up to 18% and 21% respectively over the control plants. These results indicate that sub-toxic levels of atrazine significantly stimulate plant growth. Barley plants given higher concentrations of atrazine (1.0 and 10 uM) showed up to a 78% and 42% decrease in the activity of RuDPCase and PRuK respectively as well as …

The Enzymatic Oxidation Of Aldehydes By Xanthine Oxidase, Duane Beal Gregory

All Master's Theses

As early as sixty years ago, the existence of an enzyme from mammalian tissues which catalyzed the oxidation of xanthine and hypoxanthine was recognized. The name given the enzyme was derived from its earliest recognized action, hence xanthine oxidase. Although little is known about the biological functions of the enzyme, the high affinity shown for xanthine and hypoxanthine may indicate that these are the most significant substrates biologically.

The Effects Of Sources And Levels Of Vitamin A And D On Some Aspects Of Sulfur Metabolism, Akbar Malekpour

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Two trials were conducted using 48 infant pigs of both sexes. Pigs were taken from the sow when three to seven days old and placed in wire-bottomed metal cages by pairs. Animals in the first trial received synthetic diets containing 100 or 1000 international units of ergocalciferol and 1000 or 4000 international units of retinyl acetate or four or sixteen milligrams of beta carotene per kilogram of feed. Baby pigs in the second trial received the same synthetic diet plus 1000 international units of ergocalciferol (D₂) or cholecalciferol (D₃) and 2000 international units of vitamin A …

Asymptotic Evaluation Of Wkb Matrix Elements. Ii. Use Of Langer's Uniform Asymptotic Wavefunctions, Wesley D. Smith, Russell T. Pack

Faculty Publications

An improvement in a previous procedure for the analytic asymptotic evaluation of integrals arising in the quantum-mechanical theory of inelastic molecular collisions is presented. The integrals are evaluated using Langer's uniform asymptotic wavefunctions and the higher-order saddle-point or steepest-descents method. It is found that the Langer functions give no better results than WKB functions, but the higher-order steepest-descents corrections produce a marked improvement. The result is a simple method for evaluating a large class of integrals with an error of 0.1%-2.1%.

Compartmentation Of Glutamate Metabolism Observed With Glucose / D. D. Clarke, E. Dicker & E.J. Ronan, Chem. Dept. Fordham University, Bronx, N.Y. 10458, U.S.A., Donald Dudley Clarke Phd, E. Dicker, E. J. Ronan

Chemistry Faculty Publications

Compartmentation of glutamate metabolism in the central nervous system has been demonstrated in a number of laboratories using a variety of labeled precursors. The labeling of glutamine is much more rapid than would be expected if the total pool of glutamate in brain is the precursor of glutamine. An obvious suggestion for the origin of this compartmentation is that it reflects glial vs. neuronal relationships. Some evidence in support of this idea has been obtained by Rose using separated neuronal vs. neuropil preparations (J. Neurochem. 17, 809 (1970)). Until recently orain was the only tissue which, after the injection of …

Urea Block Supplements For Stubble, H E. Fels, R. J. Parkin

Journal of the Department of Agriculture, Western Australia, Series 4

THERE was a great deal of controversy throughout the summer of Western Australia's 1969-70 drought over the feeding of supplements other than grain to grazing sheep.

This report summarises some of the experiments carried out by this Department to investigate various artificial means of supplementing sheep grazing stubbles and dry summer pastures.

Further details of the experiments can be obtained by contacting the authors.

The results failed to demonstrate economic responses to urea supplements for grazing sheep.

Urea Or Grain Supplements For Stubble?, H E. Fels

Journal of the Department of Agriculture, Western Australia, Series 4

Three methods of supplementing sheep grazing stubble were compared at Merredin Research Station in 1970. The stubble used in the experiment was left after harvesting a nine-bushel crop of Gamenya wheat which was a weed-free second crop on new land. The land had never been sown to legumes.

Experiments With Urea On Private Farms, R J. Parkin, G. Palmer, A. Haagensen, L. D. White, R. J. Suiter, H. E. Fels

Journal of the Department of Agriculture, Western Australia, Series 4

Many District Advisers have carried out trials on private farms to test the response to a variety of types of supplementary feeds. This report gives brief details of five such experiments carried out with urea supplements over the last five years. Table 1 summarises the details and results of these trials.

Urea Mixture Formulations, H E. Fels

Journal of the Department of Agriculture, Western Australia, Series 4

This experiment was done once the experiment above (Urea or Grain Supplements for Stubble) had shown definite but uneconomic responses to a particular mixture of urea, molasses and minerals. The aim was to find whether the response depended on the content of various portions of the mixture, and whether the response to the "shotgun" lick could be explained by the presence of salt, sulphate or cobalt.

Evaluation Of The Essentiality Of Dextran In The Dental Caries Process, William Redman Grigsby

Theses and Dissertations

In summary, the main points to support the view that dextran synthesis is unessential to the dental caries process are presented below:

1 . Dextran-fed, microbe-bearing rats do not experience dental caries.

2 . Lactobacillus casei 4646 has no extracellular dextransucrase enzyme or other extracellular enzymes which release glucose or fructose from sucrose .

3. L. casei 4646 does not synthesize extracellular polysaccharide of the dextran class when grown on sucrose-containing medium.

4. L. casei 4646 does not exhibit in vitro, adherent growth on stainless-steel wire when grown on sucrose-containing medium .

5. L. casei 4646 does not cause dental …

Characterization Of Some Unidentified Components In Aorta Lipids, Max Elvin Royer

Masters Theses

No abstract provided.

Quasilattice Interpretation Of The Thermodynamic Properties Of Mixing Of Binary Alkali-Metal Systems, J. Bevan Ott, J. Rex Goates

Faculty Publications

Quasilattice theory was applied to heats of mixing in the six systems formed from the binary combinations of Na, K, Rb, and Cs. The alkali-metal systems are particularly well suited for a critical test of the quasi-crystalline assumption since the theoretical model for these systems is relatively simple and the thermodynamic data are fairly complex. Very good fits of the heat-of-mixing data were achieved by assuming that the larger atom in the binary system covered multiple sites on the quasilattice. The values of the empirically determined constants appear to fit the physical significance accorded to them by the quasilattice model. …

Pigments And Neurogenic Secretions Of Dermasterias Imbricata, Stephen Edward Davis

All Master's Theses

Identification of the compound from the asteroid Dermasterias imbricata that evokes the swimming response in the sea anemone Stomphia coccinea was attempted. Although the compound was not identified, it was postulated to be a low molecular weight highly polar molecule of possible carbohydrate origin.

A Study Of Folic Acid Dependent Reactions In Bovine Brain, Dillard Spencer Broderick

Theses and Dissertations

The enzymatic interconversion of serine to glycine and N5, N10-methylene tetrahydrofolate to N5 -methyl tetrahydrofolate have been studied in a cell-free system derived from bovine brain. A partial purification of serine transhydroxymethylase and N5, N10 -methylene tetrahydrofolate reductase has been achieved by ammonium sulfate fractionation, protamine sulfate fraction, and by various column chromatography procedures. The two enzymes studied catalyze sequentially the reductive transfer of the hydroxymethyl group of serine to the N5 position of tetrahydrofolate forming N5 -methyl tetrahydrofolate. The products of these reactions were assayed by the incorporation of C14 from a one-carbon precursor i.e., L-beta-(C14)-serine, or N5 -methyl …

Gas Chromatographic Determination Of Changes In Concentration Of Pipecolic Acid And Other Free Amino Acids In Hypersensitive Tobacco Plants Induced By Tobacco Mosaic Virus And Temperature, David Francis Brown

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Changes in concentrations of pipecolic acid and other free amino acids in Nicotiana tabacum, L. cv. Xanthi induced by tobacco mosaic virus (TMV) incubated at 75 or 95 F after inoculation were investigated gas chromatographically. A preliminary extraction and gas chromatographic technique for separation and quantitative determination of the free amino acids, using hydroxyproline as an internal standard, was developed.

Amino acids were converted into their trifluoroacetyl n-butyl (TAB) derivatives and gas chromatographed on 0.325 percent ethylene glycol adipate (EGA) columns. Resolution was generally good, but glycine-isoleucine and phenylalanine-aspartic acids were not resolved on old columns. The over-all …

Quantitative Evaluation Of Saponin Content In Du Puits Alfalfa Foliage (Medicago Sativa L.) With Trichoderma, Wang Li-Chun

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Selections in Du Puits alfalfa (Medicago sativa L. ) were evaluated for saponin content. The data obtained from the Trichoderma bioassay were converted to saponin values by regressions and standard curves. It was necessary to adjust the concentration of alfalfa extract in the bioassay so that the saponin content in alfalfa was estimated in that portion of the curve showing a marked response to saponin. A different regression was established for each group of low and high saponin plants of Du Puits alfalfa. The standard curve prepared with extract of Du Puits alfalfa used on the basis of its …

The Nature Of The "Contractile" Protein Isolated From Brain / S. Puszkin, E. Puszkin, D. D. Clarke, And S. Berl. Coll Of Phys. And Surgeons., Columbia Univ., N.Y. 10032, S. Puszkin, E. Puszkin, Donald Dudley Clarke Phd, Soll Berl

Chemistry Faculty Publications

We have described the isolation from brain of a Mg++/Ca++-ATPase resembling muscle actomyosin (Sci. 12 1 1701 1968). Like myofibrils (Perry and Grey 1 Biochem. J. 64, 184, 1956), the ATPase activity isolated from rat and cat brain is dependent upon the cone. of ATP and cation. When the conc. of ATP exceeds that of Mg++ 1 enzyme activity is inhibited. With increasing conc. of Mg++ 1 the optimum conc. of ATP also increases. This does not occur with Ca++ . Polyethylenesulfonate( PS)(I0-6M)causes the splitting of actomyosin into actin and myosin; the Mg++-ATPose activity is correspondingly diminished (Barony and Joisle …