Life Sciences Commons^™

Cow Brain Glutaminase: Purification, Characterization, And Mechanism Of Action, Fulung John Chiu

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A simple, quick, and sensitive radiometric assay for glutaminase has been established. This assay uses L-(U-¹⁴C)-glutamine as the substrate and measures the ¹⁴CO₂ produced when the reaction is coupled to glutamate decarboxylase. An efficient purification procedure for cow brain glutaminase has also been developed; the yield is 12%. Steps include acetone extraction, ammonium sulfate fractionation, calcium phosphate gel treatment, and differential gel filtration on Sepharose 4B. The final preparation has a specific activity of 385 μmole/min/mg and shows a single protein band on polyacrylamide gel electrophoresis in sodium dodecyl sulfate; this band corresponds to a …

Fractionation Of Ribosomal Proteins By Affinity Chromatography, Corazon E. Victoria

Masters Theses

No abstract provided.

Steriodogenesis In Mouse Preimplantation Embryos, Marcia E. Kendall

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Marcia E. Kendall February 8, 1979.

Synthesis Of 2-Aminomorphine And 2-Aminocodeine. Reduction Of Aromatic Nitro Groups With Formamidinesulfinic Acid. / Nithiananda Chatterjie, Arlene Minar And Donald D. Clarke New York State Institute For Basic Research In Mental Retardation, Staten Island, Ny 10314, Nithiananda Chatterjie, Arlene Minar, Donald Dudley Clarke Phd

Chemistry Faculty Publications

Our interest in the stereoselective reduction of the carbonyl groups of various oxymorphone derivatives with formamidinesulfinic acid (FSA) led to a result indicating that dihydrocodeinone underwent ring-oxygen scission under the conditions of reduction with this reagent. This observation4 has prompted interest and speculation as to the scope and mechanism of this reaction by others, as well as ourselves

Photoelectric Properties And Detection Of The Aromatic Carcinogens Benzo[A]Pyrene And Dimethylbenzanthracene, William A. Houle, Hugh M. Brown, O. Hayes Griffith

Department of Biochemistry: Faculty Publications

The absolute photoelectron quantum yield spectra for benzo[ajpyrene and dimethylbenzanthracene are presented in the wavelength range 180-230 nm. These polycyclic aromatic carcinogens have photoelectron quantum yields of approximately 2 X 10^-3 electrons per incident photon at 180 nm. The quantum yields fall off quickly and monotonically at wavelengths longer than 210 nm (5.9 eV). Threshold values for benzo[a]pyrene and dimethylbenzanthracene are 5.25 ± 0.06 eV and 5.27 ± 0.04 eV, respectively. The photoelectron quantum yields of benzo[a]pyrene and dimethylben-zanthracene are several orders of magnitude greater than typical components of biological membranes (amino acids, phospholipids, and polysaccharides). Preliminary micrographs of …

Potential Use Of Hydroelectric Facilities For Manipulating The Fertility Of Lake Mead, Larry J. Paulson, John R. Baker, James E. Deacon

Publications (WR)

Analysis of historical nutrient data for Lake Mead indicates that the fertility of the reservoir has decreased which may be the cause for a corresponding decline in the largemouth bass population. However, it appears that fertility can be manipulated by altering the operation of the dam. The depletion of nutrients in the euphotic zone by phytoplankton and subsequent accumulation in the hypolimnion during summer and fall provide a natural nutrient gradient from which water of varying fertility can be drawn for discharge. This combined with alterations in the depth or seasonal pattern of discharge can possibly be used to enhance …

Pulling The Wool Off, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

The Department of Agriculture has begun a three year project to study the potential for "chemical shearing" or, more correctly, biological defleecing.

The Purification, Properties And Chemical Kinetics Of Cathepsin B1, Andrew Stanley Bajkowski

Master's Theses

No abstract provided.

Dna Replication In Mesophilic And Thermophilic Bacteria, Pawan K. Gupta

Dissertations

No abstract provided.

An Emulsion Dye Laser, Kenneth Lee Matheson, James M. Thorne

Faculty Publications

A laser dye which is insoluble in water has been dissolved in hexane and emulsified in a water matrix. When pumped with a nitrogen laser, this mixture was observed to lase. The emulsion is superior to a simple hexane solution because the excellent thermo-optical properties of the water matrix help prevent refractive-index gradients from degrading laser performance. This is a useful characteristic for flash-pumped dye lasers, laser-pumped dye lasers, and liquid filters. Another type of solvent system, a critical solution, is also discussed. For certain dyes, a critical solution has even better thermo-optical properties because of its ability to absorb …

Non-Parental-Type Recombinants In Crosses Between Different Restriction-Modification Systems In Salmonella, Mary Ball

Loma Linda University Electronic Theses, Dissertations & Projects

Many new restriction-modification (R-M) system which are closely linked to serB and allelic to the hsd_SB system of S. typimurium occur in different Salmonella serotypes. In PI transduction studies with these systems, recombinants were isolated which fell into one of three classes with reference to their specific restriction phenotype. Recombinants that restricted like the donor, recombinants that restricted like the recipient and recombinants that had lost the ability to restrict. This latter group having lost the recipient SB restriction without gaining the specific donor restriction and here called "zero recombinants,” were of special interest and were investigated.

Zero recombinants …

The Effect Of Selenium On The Toxicity Of Methyl Mercury In Medaka Embryos, Peter H. Krauth

All-Inclusive List of Electronic Theses and Dissertations

The purpose of this research was to investigate the effect of selenium upon methyl mercury poisoning in Medaka fish (Oryzias latipes) embryos. Selenium has been shown to provide a protective mechanism against mercury poisoning in some adult animals. Three experiments were done. In the first, selenium and methyl mercury were put directly in the growing medium of the eggs. In the second experiment, selenium was included in the diet of the parent fish. In the third part, methyl mercury exposure was delayed three days. The results of the first experiment were quite dramatic and showed a significant difference when selenium …

A Unified Radiometric Assay System For The Gaba-Glutamate Regulating Enzymes, Robert C. Dinwoodie

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this paper was to develop a single assay system for the enzymes which regulate GABA and glutamate concentrations in brain and nerve tissue. Since all the enzymes produce L-glutamate, their activities were measured by coupling them to L-glutamate decarboxylase. Enzymatic activity was determined by measuring the release of co2 from radioactive substrates. The glutamate decarboxylase was obtained from a commercial acetone powder by simplifying existing procedures. The glutamate decarboxylase produced was of sufficient purity to be used in the coupled assays, which were checked with commercial preparations of each enzyme, where available, and with crude brain homogenates. …

Amino Acid Analogs Iv: 4-Fluoroisoleucine / Herman Gershon, Larry Shanks, And Donald D. Clark, Herman Gershon, Larry Shanks, Donald Dudley Clarke Phd

Chemistry Faculty Publications

4-Fluoroisoleucine was produced by ammonolysis of 2- bromo-4-fluoro-3-methylpentanoic acid, which resulted from the bromofluorination of 4-methyl-2-pentenoic acid. It did not inhibit Plasmodium berghei in mice at 640 mg/kg and was not toxic to the animals. The fluoroamino acid inhibited Aspergillus niger, Trichoderma viride, Myrothecium verrucaria, Trichophyton mentagrophytes, and Mucor mucedo in Czapek solution agar at a concentration between 104 and 103 μg/ml. Growth of Escherichia coli was inhibited 25% at 900 μg/ml in a defined medium

Glial Cells And Metabolic Compartmentation / S. Berl, W.J. Nicklas, And D.D. Clarke Dept. Of Neurology, Mt. Sinai School Of Medicine, N.Y., N.Y. 10029 Dept. Of Chemistry, Fordham University, N.Y. 10458, U.S.A., Soll Berl, William J. Nicklas, Donald Dudley Clarke Phd

Chemistry Faculty Publications

The study of metabolic compartmentation in brain has been under investigation for some twenty years. Most interpretations of the data have focused on attempts to assess the relative contributions of glia and neurons to the metabolic pictures since anatomically and functionally they are distinct. It is very probable that these cell types contain other metabolic compartments based on anatomic and subcellular subdivisions. However, the presently available experimental data is insufficient to specify precisely the total number of such metabolic pools functioning in nervous tissue. The initial observations that set in motion most of the work were a) in the adult …

An Immunochemical Study Of The Combining Sites Of The Second Lectin Isolated From Bandeiraea Simplicifolia (Bs Ii), Charles Wood, E. A. Kabat, S. Ebisu, Irwin J. Goldstein

Nebraska Center for Virology: Faculty Publications

Two lectins with different binding specificities have been isolated from extracts of seeds of Bandeiraea simplicifolia. The first, Bandeiraea lectin I [11] was specific for terminal non-reducing αDGalactosyl residues. It reacted with B substances from human ovarian cysts and with several galactomannans to form precipitin lines in agar gels. Polysaccharides with terminal αDGalactosyl residues, such as larch galactan, did not react. The lectin agglutinated B erythrocytes strongly but also reacted to a lower titre with A₁ and very weakly with A₂ erythrocytes [15, 28] indicating that terminal non-reducing αDGalNAc [24] can …

Investigations Of Possible Allelopathic Effects Of Lycopodium Flabelliforme (Fern.) Blanch., Joan Macuszek

Masters Theses

Lycopodium flabelliforme (Fern.) Blanch. sporophytes were collected from a colony west of Flat Rock in Crawford County, Illinois. The material was either ground to a coarse grade using a Wiley Mill (grindate), left whole and untreated (fresh) or water extracted with a Waring blender (extract). The effect of the sporophyte material was tested on the germination of seeds and on the growth of seedlings of Raphnus sativa L. Sporophytes subjected to the grindate treatment showed the most inhibitory effects. It was shown that the alledged inhibitory substance was heat stable and able to be concentrated. Using column chromatography, at 37.7°C, …

Aluminum Metabolism In Health And Disease, Jay Eliot Gorsky

Dissertations

No abstract provided.

The Interaction Of Thioxanthenones With Nucleic Acids, Laszlo Bodoni

Dissertations

No abstract provided.

The Metabolism Of Linolenic Acid In Developing Rat Brain, Barney E. Dwyer

Dissertations

No abstract provided.

Aldehydic Transition State Analogue Inhibitors Of Α-Chymotrypsin, Alexandra Constance Cheerva

Master's Theses

No abstract provided.

The Effect Of Metabolites, Which Accumulate During Phenylketonuria, On The Activity Of Stearoyl-Coenzyme A Desaturase, Walter Scott

Masters Theses

No abstract provided.

Comparison Of Protein Kinase Activity In Tissue Extracts Of The Diabetic And Nondiabetic Chinese Hamster, Michael Andrew Dombos

Masters Theses

No abstract provided.

The Half-Life Of Bacterial Messenger Rna, John B. Madison

Masters Theses

No abstract provided.

Isolation, Purification And Partial Characterization Of Some Isoenzymes Of Human Serum Alkaline Phosphatase, M. Sue Jones

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science by M. Sue Jones on July 18, 1977.

Light Scattering Studies Of Transverse Sound Wave And Molecular Motion In Benzonitrile, Scott L. Whittenburg, C. H. Wang

Chemistry and Biochemistry Faculty Publications

The zero‐frequency shear wave dip appearing in the depolarized Rayleigh spectrum in benzonitrile has been studied as a function of concentration and temperature. The solution study was carried out at constant viscosity equal to the viscosity of liquid benzonitrile at each temperature. The result indicates that the presence of shear wave fine structure does not depend on the collective orientational fluctuations. The orientational and vibrational relaxation times of benzonitrile were measured at various concentrations and temperatures. The orientational relaxation times show no concentration dependence at any temperature, suggesting that the pair correlation is negligible at all concentrations. The orientational relaxation …

Limnological Aspects Of Lake Mead, Nevada-Arizona, John R. Baker, James E. Deacon, Thomas A. Burke, Samuel S. Egdorf, Larry J. Paulson, Richard W. Tew, Bureau Of Reclamation

Publications (WR)

Lake Mead is a deep, subtropical, moderately productive, desert impoundment with a negative heterograde oxygen profile occurring during; the summer stratification. investigations of the Boulder Basin of Lake Mead by the University of Nevada were initiated in November 1971. The primary objective of the study was to determine what effects industrial and sewage effluent from the Las Vegas metropolitan area, discharged into Las Vegas Bay, have had on the water quality and limnological conditions of Boulder Basin. Data from the 1975-76 period are presented in detail, with earlier data included in the summaries and discussions. Measurements of water temperature, dissolved …

Human Placental Alkaline Phosphatase And Steroid Metabolism By Rat Adrenals, Allen G. Meier

Loma Linda University Electronic Theses, Dissertations & Projects

Human placental alkaline phosphatase was purified by butanol extraction, methanol precipitation and chromatography on DEAE-cellulose. The pH optimum was found to be 10.9. The enzyme was competitively inhibited by di-ethyl-p-nitrobenzyl-phosphonate and copper sulfate. An increase in enzymatic activity occurred in the presence of the phosphate ion (HPO₄) and ATP.

The enzyme was crystallized from a 40% saturated solution of ammonium sulfate and shown to be homogeneous by sedimentation analysis. Homogeneity of the enzyme was also demonstrated using poly-acrylamide-gel-electrophoresis. At a pH of 7.5% the electrophoretic pattern yielded a single band and showed no significant contamination.

The metabolic transformation …

The Relationship Of Reduced Levels Of Atp To Inhibited Chondrogenesis In The Production Of Limb Deformity Induced By 6-Aminonicotinamide, Val C. Sheffield

Theses and Dissertations

6-aminonicotinamide (6-AN), an analogue and antagonist of nicotinamide, impairs cartilage formation and results in shortening of the limb when administered to chick embryos. 6-AN forms an abnormal NAD analogue (6-ANAD), which inhibits the activity of NAD-dependent enzymes associated with the production of ATP. The mechanism of action of 6-AN was studied by measuring the biosynthesis of protein and DNA, and the sulfation and glycosylation of chondroitin sulfate in control and 6-AN treated cartilage from 8-day chick embryos. The cartilage was also assayed for ATP levels. Incorporation of Na235SO4 was inhibited by 6 h treatment with 10 μg/ml 6-AN, whereas 3H-thymidine …

Light Scattering Studies Of Rotational And Vibrational Relaxations Of Acetonitrile In Carbon Tetrachloride, Scott L. Whittenburg, C. H. Wang

Chemistry and Biochemistry Faculty Publications

The rotational and vibrational relaxation times of acetonitrile–carbon tetrachloride solutions were investigated as a function of concentration, viscosity, and temperature using depolarized Rayleigh and Raman scattering. Using a Fabry‐Perot interferometer and single frequency laser source, we have shown that reliable results for the single particle orientational correlation times (τ_s) for CH₃CN can be obtained by carrying out a concentration dependent depolarized Rayleigh scattering study. Raman scattering was shown to yield inconsistent results for τ_s in CH₃CN. At constant viscosity, it was found that the Rayleigh scatteringrelaxation time (τ_Ray) of CH_{3 …}