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Articles 1 - 12 of 12
Full-Text Articles in Life Sciences
Development Of A [2]-Catenane Synthetic Method And A Student Beliefs Survey For A Hybrid Organometallics Course, Jourdan E. Lakes
Development Of A [2]-Catenane Synthetic Method And A Student Beliefs Survey For A Hybrid Organometallics Course, Jourdan E. Lakes
Honors Theses
In this study, the value-added of a new upper-level chemistry course, CHEM 372-Organometallics, was defined using a Student Beliefs Survey that was answered by students in CHEM 372 and by faculty who teach upper-level chemistry courses at Coastal Carolina University. The laboratory portion of CHEM 372 focused on the synthesis of a [2]-catenane, which is a molecularly interlocked molecule (MIM). The intention was to give the CHEM 372 students a goal to work toward over the course of the semester instead of different, unrelated, experiments each week. Using this style of lab teaching, the students were introduced to graduate school-level …
An Analysis Of Environmentally-Persistent Free Radical Formation On Surrogate Soil Systems Containing Iron(Iii) And Copper(Ii) And The Potential Remediation Effects Of Laccases, Andrew Paul Mullet
An Analysis Of Environmentally-Persistent Free Radical Formation On Surrogate Soil Systems Containing Iron(Iii) And Copper(Ii) And The Potential Remediation Effects Of Laccases, Andrew Paul Mullet
Honors Theses
No abstract provided.
Developing A Deletion Construct Of The Halothiobacillus Neapolitanus Csos1c Gene, Ellyn K. Dunbar
Developing A Deletion Construct Of The Halothiobacillus Neapolitanus Csos1c Gene, Ellyn K. Dunbar
Honors Theses
The purpose of this research was to develop a deletion construct for the chemoautotrophic bacterium Halothiobacillus neapolitanus, which will be used to generate a mutant lacking a carboxysome shell protein gene. The carboxysome is the location of carbon dioxide fixation. The operon that encodes the carboxysome contains three genes for CsoS1 proteins, the major components of the carboxysome shell. The small CsoS1 proteins self-assemble into hexamers with small central pores. The hexamers arrange into the facets of the icosahedral carboxysome shell. The pores are believed to be involved in selective diffusion of materials necessary for carbon dioxide fixation across the …
Effects Of Creatine, Leucine, And Ethanol On Glial Cell Line-Derived Neurotrophic Factor In C2c12 Skeletal Muscle Cells, Daniel Waters
Effects Of Creatine, Leucine, And Ethanol On Glial Cell Line-Derived Neurotrophic Factor In C2c12 Skeletal Muscle Cells, Daniel Waters
Honors Theses
Glial Cell Line-Derived Neurotrophic Factor (GDNF) is a protein expressed throughout the mammalian nervous system that serves several protective functions. Originally discovered in the brain, where it supports the survival of dopaminergic neurons, GDNF has since been shown to be expressed in skeletal muscle and is vital for the maintenance of motor neurons. The relationship between muscle hypertrophy, and GDNF is not well understood. The purpose of this study was to examine the relationship between hypertrophy and GDNF by inducing hypertrophy in C2C12 skeletal muscle cells using leucine and creatine, inhibiting hypertrophy using ethanol and measuring GDNF content. As measured …
A Guided Inquiry To The Bromination Of Alkenes: An Effort To Foster Student Understanding Of Thin Layer Chromatography In The Organic Chemistry Laboratory, Casey Wright
Honors Theses
Many of the chemistry laboratories taught at Western Michigan University are “cookbook” labs, meaning that students are given an instruction manual with a prescribed set of instructions and a predicted outcome. Therein we present an introductory organic chemistry laboratory experiment to reinforce the student understanding of Thin Layer Chromatography which utilizes a guided inquiry format as part of an effort to move toward discovery based learning in our organic chemistry curriculum. Educational research was performed on classroom artifacts collected from the laboratory. We were able to gain an understanding of the struggles students face in applying general chemistry content knowledge …
Determining The Concentration Of Nitric Oxide In Solvent, Emily-Jean E. Bankes
Determining The Concentration Of Nitric Oxide In Solvent, Emily-Jean E. Bankes
Honors Theses
The body creates nitric oxide (NO) for signaling. One way that researchers study NO signaling is through the use of model complexes, or molecules that have the same metal ion and a similar molecular shape as biological molecules and are easy to synthesize. Currently researchers have limited ways to deliver precisely-known small quantities of NO to an experiment where they are trying to investigate NO-binding to a transition metal complex. A peak shift in the absorption spectrum at ~530 nm makes it possible for UV-Vis spectroscopy to observe NO binding to cobalt tetraphenylporphyrin (CoTPP). This makes it possible to measure …
Development Of A Chemical Genetic Screen To Determine Synergistic Compounds With Laromustine In Treating Glioblastoma Multiforme Cultured Cells, Ryan Weeks
Honors Theses
Laromustine is a chemotherapeutic sulfonylhydrazine prodrug used in clinical trials to treat acute myeloid leukemia (AML) and glioblastoma multiforme (GBM). While treatment of AML with laromustine has more demonstrative clinical success, there are enough promising data against GBM to pursue additional pre-clinical and clinical experiments. To determine the synergistic effects caused by treating cultured GBM cells with laromustine and a library of FDA-approved compounds, a chemical genetic screen was developed. To optimize the screen, optimal cultured GBM cell seed density, growth period and maximum well capacity were determined. The treatment period for a lethal dose of laromustine in cultured GBM …
Discovering New Antibiotics: Bacterial Extracts Separated By Thin-Layer Chromatography Inhibit The Growth Of Staphylococcus, Heidi Hughes
Discovering New Antibiotics: Bacterial Extracts Separated By Thin-Layer Chromatography Inhibit The Growth Of Staphylococcus, Heidi Hughes
Honors Theses
Many bacteria have become resistant to commonly used antibiotics because of antibiotic use in people and animals. Therefore, new antibiotics are needed that will inhibit these resistant bacteria. Bacteria found in soil are a likely source for new antibiotics because of the limited available nutrients found in the soil. We isolated soil bacteria and screened them for antibiotic production against Staphylococcus epidermidids. Methanol extracts were made from entire agar plates of the soil bacteria that inhibited S. epidermidis. These extracts were spotted on a lawn of Staphylococcus aureus; growth inhibition was measured to comfirm that the extracts contained the …
Computational Studies Of Paradifluorobenzene Cations And Hydrogen Cyanide Molecule, John C. Rowe Iv
Computational Studies Of Paradifluorobenzene Cations And Hydrogen Cyanide Molecule, John C. Rowe Iv
Honors Theses
Polycyclic aromatic hydrocarbons (PAHs) are hypothesized to comprise a significant portion of interstellar carbon identified from the Diffuse Interstellar Bands (DIBs). Gas phase cation-molecule reactions between compounds that previously existed as weakly bound species provides the best explanation for the emergence of PAHs in the interstellar medium (ISM). In this work, we use computational methods to characterize one of these weakly-bound systems. We discovered that intramolecular charge distribution (calculated using the Natural Population Analysis) dictate the complexes formed between a paradifluorobenzene cation and either one or two HCN molecules. Additionally, the relative stabilities of the complexes and the binding energies …
Characterization Of Catecholamine Receptors And Transporters In Murine Macrophages, Elizabeth Gonye
Characterization Of Catecholamine Receptors And Transporters In Murine Macrophages, Elizabeth Gonye
Honors Theses
Macrophages are a critical part of the immune response. When circulating monocytes move into tissues they differentiate int macrophages to mount the first line of defense against pathogens.
Impact Of Vector Range Expansion On Pathogen Transmission Dynamics Of Lyme Disease In Southwestern Virginia, Bishan Bhattarai
Impact Of Vector Range Expansion On Pathogen Transmission Dynamics Of Lyme Disease In Southwestern Virginia, Bishan Bhattarai
Honors Theses
Blacklegged ticks, Ixodes scapularis is the primary vector of Lyme disease in eastern United States. Borrelia Burgdorfei, the etiological agent of Lyme disease is transferred by ticks of Ixodes species. In recent years, its congener, Ixodes affinis has been expanding its range northwards from its southern population. We were interested in studying how the introduction of this new vector affected the interaction between the pathogen genotype and the host. We hypothesized that differential host use by I. affinis and I. scapularis would partly explain observed differences in B. burgdorferi infection prevalence and genotypic structure in southeastern Virginia. The result …
Calcium (Ii) Catalyzed Nitrone Additions, Elizabeth A. Congdon
Calcium (Ii) Catalyzed Nitrone Additions, Elizabeth A. Congdon
Honors Theses
Calcium(II) complexes have be shown to be successful catalysts for nitrone reactions. The addition of n-‐methyl and n-‐phenyl nitrones to donor-‐acceptor cyclopropanes was achieved with calcium triflate (Ca(OTf). Differentially substituted tetrahydro-‐1,2-‐oxazines were synthesized in good to excellent yields. Calcium triflate was also found to catalyze the addition of silyl enol ethers to n-‐phenyl nitrones along the Mukaiyama-‐Mannich addition pathway. B-‐amino carbonyls were synthesized from a variety of substituted nitrones. Bulky and cyclic silyl enol ethers were also found to be reactive, the products of which were isolated in good to excellent yields.