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Full-Text Articles in Life Sciences

Effects Of Polydatin On In Vitro Bovine Embryo Developmental Competence, Metabolism, And Cryopreservation, Corie Marie Owen Aug 2018

Effects Of Polydatin On In Vitro Bovine Embryo Developmental Competence, Metabolism, And Cryopreservation, Corie Marie Owen

Master's Theses

Bovine in vitro produced embryos suffer from poor developmental competence and altered metabolism which hinders their cryotolerance. Overall, the goals of this thesis were to improve oocyte and embryo culture with the antioxidant polydatin and to optimize slow freezing procedures. This thesis was designed as three experiments, and in each experiment, oocytes were aspirated from abattoir ovaries, matured for 23h, fertilized with semen from 1 of 3 bulls, and cultured in synthetic oviductal (SOF) based medium (SCF1) in 38.5 °C in 5% O2, 5% CO2 and 90% N2. Stage 7 blastocysts were stained with Nile …


Contractile Response Of Bovine Lateral Saphenous Vein To Ergotamine Tartrate Exposed To Different Concentrations Of Molecularly Imprinted Polymer, Manoj B. Kudupoje, James L. Klotz, Alexandros Yiannikouris, Karl A. Dawson, Kyle R. Mcleod, Eric S. Vanzant Feb 2018

Contractile Response Of Bovine Lateral Saphenous Vein To Ergotamine Tartrate Exposed To Different Concentrations Of Molecularly Imprinted Polymer, Manoj B. Kudupoje, James L. Klotz, Alexandros Yiannikouris, Karl A. Dawson, Kyle R. Mcleod, Eric S. Vanzant

Animal and Food Sciences Faculty Publications

Ergot alkaloids, in their active isomeric form, affect animal health and performance, and adsorbents are used to mitigate toxicities by reducing bioavailability. Adsorbents with high specificity (molecularly imprinted polymers: MIP) adsorb ergot alkaloids in vitro, but require evaluation for biological implications. Using ex vivo myography, synthetic polymers were evaluated for effects on the bioactivity of ergotamine tartrate (ETA). Polymers were first evaluated using isotherms. Lateral saphenous veins were collected from 17 steers for four independent studies: dose response of ETA, adsorbent dose response, validation of pre-myograph incubation conditions and MIP/ non-molecularly imprinted polymer (NIP) comparison. Norepinephrine normalized percent contractile response …


Effects Of Various Anthelmintic Treatments On Fecal Egg Counts, Performance Variables, Blood Parameters, And Lactate Dehydrogenase Activity In Stocker Calves, Cheyenne Auburn Hooks Jan 2018

Effects Of Various Anthelmintic Treatments On Fecal Egg Counts, Performance Variables, Blood Parameters, And Lactate Dehydrogenase Activity In Stocker Calves, Cheyenne Auburn Hooks

Murray State Theses and Dissertations

Anthelmintic treatment is known to improve cattle performance; however, effects of long-acting eprinomectin (LAE) and co-treatment (Co-Trt) use has not been widely evaluated. Lactate dehydrogenase is an important cytoplasmic enzyme which can serve as an indicator of cellular damage but little information is available regarding differences in activity of calves receiving various anthelmintics. Therefore, the objective of this study was to evaluate the effects of various anthelmintic treatments on fecal egg counts (FEC), performance, blood parameters, and lactate dehydrogenase (LDH) activity of newly received stocker calves. This study consisted of 125 Angus based cross bred steers grazed during summer months …