Life Sciences Commons^™

The Effects Of Ammonia On The Functionality Of Bovine Granulosa Cells, Micah Henrie

All Graduate Theses and Dissertations, Fall 2023 to Present

It is common practice in the livestock animal production industries to feed high levels of protein to maximize the yield of each animal in production. However, diets high in protein can cause decreased reproductive success within these animals. The causes behind this decrease are not fully understood. Increased dietary protein leads to increased levels of both ammonia and urea in the blood of the animal. These increased metabolite levels may be a possible explanation for the decline in reproductive success. The goal of this study is to identify how various levels of ammonia affect the function of bovine granulosa cells …

Evaluating The Role Of The Bovine Vaginal Microbiome In Neonatal And Maternal Health Outcomes, Riley D. Messman

Theses and Dissertations

The dam vaginal microbiota is the first major microbial inoculating community within the neonate. The composition of the dam vaginal microbiota has implications in calf commensal microbiota development. Alterations of the dam microbial community prior to parturition could alter inoculating communities and immune responses in both the dam and calf. Thus, authors aimed to elucidate the microbial community composition of the bovine dam vaginal and calf nasal microbiota post-partum after utilizing betadine lavages (BL). The dam vaginal and calf nasal microbial communities and immune responses were evaluated at 0-, 15-, 30- and 60-day post-partum. Microbiota composition of the dam haircoat, …

Identification Of Genes Expressed In The Anterior Pituitary Associated With Water Restriction In Beef Cattle, Himali Chathurika Wickramasinghe Vithana Arachchilage

Electronic Theses and Dissertations

Water is an essential nutrient and is required for growth, metabolism, lactation, and reproduction. Without water, livestock production would not be possible. Despite its importance, water requirements in livestock have not received much attention from the research community since the 1950s. The most recent edition of the Nutrient Requirements of Beef Cattle (8th edition) relies on literature from the 1950s or earlier for defining water requirements in beef cattle. Since the time these requirements were estimated, much has changed with regards to body size, genetics, and productivity of our livestock herds, including beef cattle. Global surface temperatures have increased in …

An Evaluation Of Bovine Reproductive Function From The Microrna Expression In The Female To Performance Testing In The Male, Erin Amiss

All Theses

Reproductive function is crucial to the success of a beef or dairy herd. Both the female and male play significant roles in the outcome of bovine reproduction. The bull contributes to reproductive success in many ways, such as genetics. The initial role that the bull has in reproductive success is fertilization, whether natural or by artificial insemination. One method of assessing bull performance are central bull test stations, which are traditionally administered by a university, extension center, or are privately owned. Central bull tests assess the performance of bulls from various management systems, breeds, and ages. Some measurements taken during …

Maternal Nutrient Restriction And Melatonin Supplementation Alter Neurotransmitter Pathways In Bovine Fetal And Placental Tissues, Allison R. Harman

Theses and Dissertations

Nutrient restriction is a relatively common production insult to pregnancy in cattle. Recently, melatonin supplementation has been investigated as a possible therapeutic to rescue the negative effects of nutrient restriction. Neurotransmitters have been implicated as having negative programming effects in mouse and human pregnancies, manifested as metabolic and neurologic disorders. The role of neurotransmitters in fetal development has only begun to be understood in mice and humans. Neurotransmitters have not been analyzed in cattle, much less within the context of a compromised pregnancy. Brangus heifers were allotted to one of four treatments (ADQ-CON, RES-CON, ADQ-MEL, RES-MEL) in either Fall 2019 …

Using Cytoplasmic Biopsies To Determine Bovine Oocyte Quality, Madison Lindsey

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Embryos resulting from assisted reproductive technologies, such as in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI), develop with lower efficiencies than embryos resulting from their in vivo counterparts. The reasons behind the developmental discrepancies remain largely unknown. Because the egg is the primary determinant of embryo developmental success, it is reasonable to consider inherent egg quality as a possible cause. The hypothesis for this project is that there are distinct mRNA transcript patterns, or molecular “fingerprints,” that distinguish high- versus low-quality eggs developing within the same environment. In this study, a small cytoplasmic biopsy was removed from 40 eggs …

Wc1 And Tcr Interactions For Γδ T Cell Activation, Alexandria Gillespie

Doctoral Dissertations

Major subpopulations of gamma delta T cells within ruminant and pigs are defined by expression of WC1, a hybrid pattern recognition receptor/co-receptor to the T cell receptor (TCR). It is known that when WC1 is knocked down cells fail to respond. Showing that WC1 plays an active role in the stimulation of bovine gamma delta T cells. Here we explored the spatio-temporal dynamics of WC1 and TCR interaction using imaging flow cytometry and stochastic optical reconstruction microscopy. We found that in quiescent gamma delta T cells both WC1 and TCR existed in separate protein domains (protein islands) but after activation …

Effects Of Microtubule Stabilizing And Recovery Agents On Vitrified Bovine Oocytes, Emily Dzekunskas

LSU Master's Theses

Oocyte vitrification, while beneficial for research and species conservation, has limited success due to cryoinjury to the meiotic spindle. This study aimed to evaluate treatments to improve meiotic spindle recovery after vitrification by treating bovine oocytes with either a microtubule stabilizing agent, or a microtubule recovery agent. In the first two experiments, Taxol and epothilone B were used to treat bovine oocytes before vitrification. Both compounds have microtubule stabilizing properties and are known antimitotic compounds commonly used in cancer treatment. Oocytes were incubated with one of the microtubule stabilizing agents for 15 minutes before vitrification at one of three concentrations …

Monocyte Abundance And White Blood Cell Expression Of Prolactin And Nf-Kb Pathway Genes In Beef Steers Are Altered By Grazing Toxic Endophyte-Infected Tall Fescue And Lipopolysaccharide Challenge, Cecilia Winfrey

Theses and Dissertations--Animal and Food Sciences

Experiment 1 tested the hypothesis that ad libitum consumption of a 1:1 blend (MIX) of sodium selenite (ISe) and SELPLEX vs solely ISe as selenium (Se) supplements (27 ppm) in a vitamin-mineral mix (V-M) by steers subjected to summer-long grazing of toxic endophyte-infected tall fescue (TE) would ameliorate the negative effects of TE by positively affecting the expression pattern of prolactin (PRL) and NF-kB pathway genes by circulating leukocytes (WBC). Experiment 2 tested the hypothesis that the MIX form of supplemental Se also would ameliorate the negative effects of in vitro blood lipopolysaccharide challenge (LPS) on WBC gene expression. The …

Sperm Decondensation And Male Pronuclear Formation In Bovine Intracytoplasmic Sperm Injection, Lauren Gatenby

LSU Master's Theses

This study assessed the effects both DTT and progesterone have on bovine spermatozoa to induce decondensation or the acrosome reaction in order to facilitate male pronuclear formation after ICSI. Sperm prepared by swim-up in a 5 mM concentration of DTT displayed time dependent morphological changes resulting in decondensation over a 6-hour period. An estimated 90% of treated sperm displayed early changes in morphology after the second hour of incubation. Sperm displaying partial decondensation of the nucleus was estimated as 42% by the 3^rd hour and increased to 62% by the 5^th hour of incubation. Fully decondensed sperm or …

Maturation And Culture Media Effects On In Vitro Bovine Embryo Developmental Competence, Ciara M. Helland

Master's Theses

In vitro produced bovine embryos are critical to the cattle industry. However, these embryos have altered morphology, epigenetics, and metabolism when compared to their in vivo counterparts. The aim of this thesis was to alter maturation and culture media to improve the developmental competence of in vitro bovine embryos. This thesis is comprised of three experiments and one proof of concept study. Each experiment followed the same general layout: oocyte aspiration from Jersey or Holstein ovaries, oocyte maturation for 24 hours, fertilization with bull semen for 24 hours, then embryo culture for 7-8 days in 38.5 °C in 5% O …

Impact Of Ergot Alkaloid And Estradiol 17b On Whole-Body Protein Turnover And Expression Of Mtor Pathway Proteins In Muscle Of Cattle, Taylor Dawn Ferguson

Theses and Dissertations--Animal and Food Sciences

Beef cattle consuming endophyte infected tall fescue typically exhibit reduced performance in terms of both decreased dry matter intake (DMI) and growth rates. It has been suggested that lower concentrations of circulating IGF-1 (an important stimulator of the mTOR pathway and ultimately protein synthesis), as observed with consumption of ergot alkaloids, contribute to reduced growth rates. The objective of the current study was to determine if fescue-derived alkaloids decrease muscle protein synthesis though inhibitory action on the mTOR pathway via a direct effect on signal proteins, and if these negative effects can be alleviated by implantation with anabolic agents. Thirty-two …

The Role Of T-Cell Lymphoma Invasion And Metastasis 2 (Tiam2) In The Barrier Function Of Mammary Epithelial Cells Following Streptococcus Uberis Inoculation, Catherine E. Donnell

Chancellor’s Honors Program Projects

No abstract provided.

Investigation Of Transforming Growth Factor-Alpha And Its Potential Role In Promoting Ovarian Follicular Dominance, Allie Lynn Lundberg

Graduate College Dissertations and Theses

Intraovarian growth factors play a vital role in influencing the fate of ovarian follicles. They affect proliferation versus apoptosis of granulosa cells (GCs), and can influence whether small antral follicles continue their growth or undergo atresia. Transforming Growth Factor-alpha (TGFα), an oocyte-derived growth factor, is thought to regulate granulosa cell function, yet has been largely overshadowed by current interest in TGF-beta superfamily members, such as bone morphogenetic proteins (BMPs) and anti-Mullerian hormone (AMH). In the current study, effects of TGFα on bovine GC proliferation, intracellular signaling and cytokine-induced apoptosis were evaluated. Briefly, all small antral follicles (3-5mm) from bovine ovaries …

Examination Of Bovine Rumen Fluid And Milk Fat Globule Membrane Proteome Dynamics, Mallory Cate Honan

Graduate College Dissertations and Theses

Proteomic technology has been increasingly incorporated into agricultural research, as characterization of proteomes can provide valuable information for potential biomarkers of health and physiological status of an animal. As dairy cattle are a dominant production animal in the USA, their biofluids such as milk, blood, urine, and rumen fluid have been examined by proteomic analysis. The research outlined herein was performed to further characterize the dynamics of specific proteomes and relate them to dairy cattle physiology.

The first experiment evaluated the diurnal dynamicity of the rumen metaproteome in Holstein dairy cattle. Rumen fluid was collected from three mid to late …

Effects Of Polydatin On In Vitro Bovine Embryo Developmental Competence, Metabolism, And Cryopreservation, Corie Marie Owen

Master's Theses

Bovine in vitro produced embryos suffer from poor developmental competence and altered metabolism which hinders their cryotolerance. Overall, the goals of this thesis were to improve oocyte and embryo culture with the antioxidant polydatin and to optimize slow freezing procedures. This thesis was designed as three experiments, and in each experiment, oocytes were aspirated from abattoir ovaries, matured for 23h, fertilized with semen from 1 of 3 bulls, and cultured in synthetic oviductal (SOF) based medium (SCF1) in 38.5 °C in 5% O₂, 5% CO₂ and 90% N₂. Stage 7 blastocysts were stained with Nile …

Effects Of Various Anthelmintic Treatments On Fecal Egg Counts, Performance Variables, Blood Parameters, And Lactate Dehydrogenase Activity In Stocker Calves, Cheyenne Auburn Hooks

Murray State Theses and Dissertations

Anthelmintic treatment is known to improve cattle performance; however, effects of long-acting eprinomectin (LAE) and co-treatment (Co-Trt) use has not been widely evaluated. Lactate dehydrogenase is an important cytoplasmic enzyme which can serve as an indicator of cellular damage but little information is available regarding differences in activity of calves receiving various anthelmintics. Therefore, the objective of this study was to evaluate the effects of various anthelmintic treatments on fecal egg counts (FEC), performance, blood parameters, and lactate dehydrogenase (LDH) activity of newly received stocker calves. This study consisted of 125 Angus based cross bred steers grazed during summer months …

Influence Of Ergot Alkaloids On Rumen Motility: Time And Concentration Of Ergovaline + Ergovalinine Required To Impact Reticulorumen Motility, Kara Riccioni

Theses and Dissertations--Animal and Food Sciences

Fescue toxicosis is problematic for ruminant livestock, causing weight loss and low productivity when fed endophyte-infected forages. Complete underlying mechanisms of toxicosis are unknown therefore; the objective of the study was to determine if ruminally dosed ergot alkaloids impact rumen motility. Cannulated steers were pair-fed a forage diet and ruminally dosed with endophyte-free (E-) or endophyte-infected (E+) tall fescue seed. An 8-h period of rumen motility collection began 4-h after feeding by monitoring pressure change via a wireless telemetry and transducer system. In experiment 1, steers were paired by weight and assigned to E- or E+ treatment. Overall, E+ steers …

Metabolic Regulation And Cryotolerance Of In Vitro-Produced Holstein Embryos, Melissa Ann Roberts

Master's Theses

In vitro production and transfer of embryos has become a common practice within the dairy industry to efficiently breed superior animals and meet the consumption demand of the growing population. Cyropreservation is necessary for the application of commercialized embryo transfer, however, in vitro-produced embryos show morphological and physiological defects which negatively impact their ability to withstand cryopreservation in comparison to their in vivo counterparts. These artifacts result from culture conditions that cause stress to the embryo during development, leading to an accumulation of intracellular lipids, mitochondrial dysfunction, and ultimately poor ability to withstand freezing and thawing. The objective of …

Examination Of The Effects Breed And Nutrition Have On The Milk Protein Profile Produced By Lactating Dairy Cattle, Rinske Tacoma

Graduate College Dissertations and Theses

Milk is a highly nutritious natural product and research over the last 10 years has proven that these milk proteins not only provide a rich source of amino acids to the consumer but also contains many bioactive proteins and peptides known to exert biological activity benefitting human health. In this research, proteomic methods were first used to characterize the low abundance proteome within the skim milk fraction produced by Holstein and Jersey dairy cows maintained under the same diet, management and environmental conditions. Milk samples were collected over a seven day period from six Holstein and six Jersey dairy cows. …

Characterization Of Small Non-Coding Rnas In The Seminal Plasma Of Beef Bulls With Predicted High And Low Fertility, Stephanie Perkins

Electronic Theses and Dissertations

MicroRNAs (miRNAs) are a family of small non-coding RNAs (sRNA) that play a key role in regulating gene expression by binding to their complementary mRNA and altering translation. It has been reported that this down-regulation of specific areas of the genome plays a role in male fertility. Piwi-interacting RNAs (piRNAs) are another class of germline-specific non-coding RNAs that form silencing complexes to maintain germline genome integrity. In bovine seminal plasma, piRNAs have not previously been characterized. Thus, the objective of this study was to investigate a potential relationship between expression of these sRNAs and fertility in beef bulls and to …

Improving Production Efficiency Of Beef Cow-Calf Operations, Amanda Jo Davis

Graduate Theses and Dissertations

Three studies were conducted to improve and incorporate reproductive management practices into beef cow-calf production. First study evaluated serial use of Estrotect estrous detection patches as a simple, cost-effective reproductive management tool to identify cyclic animals before breeding, distinguish between females conceiving to AI versus natural service, and determine seasonal pregnancy rate after bull removal. Also determined, was effectiveness of altered timing of GnRH treatment (1 d ± CIDR removal) in a modified 14-d CIDR-Select Synch protocol. When evaluated over a 4-wk period, estrous detection patches correctly (P < 0.01) identified 79% of cyclic and 86% of non-cyclic heifers. Patches were 96 and 98% accurate in identifying heifers and cows pregnant by AI, respectively, and were 76 and 87% accurate in identifying pregnant heifers and cows at the end of the breeding season (P < 0.01). Treatment with GnRH at CIDR removal reduced labor costs and animal handling without compromising estrous response (both ~63.0%) and AI pregnancy rates (~76 and 77%; P > 0.1). Second study determined if addition of PGF2alpha treatment on d 7 …

Prediction Of Superovulatory Response In Beef Cows Based On Serum Anti-Mullerian Hormone And Antral Follicle Number, Keith Center

Graduate Theses and Dissertations

A study investigated the use of Anti-Mullerian hormone (AMH) and/or follicle counts as a predictor of subsequent superovulatory response and embryo production in 79 beef cows. Before initiation of superovulation, ultrasonography was used to scan the ovaries of each donor cow to record the number of 3 to 5 mm follicles present, and a blood sample was collected for measure of serum AMH. At the time of embryo collection, the ovaries of donor cows were palpated to estimate the number of corpora lutea (CL) present on each ovary. Recovered embryos were evaluated for stage of development and morphological quality. Across …

Impact Of Heat Stress On Germinal Vesicle Breakdown And Lipolytic Changes During In Vitro Maturation Of Bovine Oocytes, Leah Marie Hooper

Masters Theses

The main objective of this research was to examine the lipolytic changes in triglyceride and phospholipid as well as the incidence of germinal vesicle breakdown during IVM of heat-stressed oocytes compared to non-stressed oocytes. To this end, cumulus-oocyte complexes were matured for 0, 2, 4, 6 or 24 hIVM at 38.5 or 41.0°C (first 12 h only, then transferred to 38.5°C). Triglyceride and phospholipid levels decreased by 2 hIVM (P = 0.0009 and P = 0.0005, respectively) but remained fairly constant to 24 hIVM; lipid decline was not affected by maturation temperature. Elevated maturation temperature hastened meiotic progression by 4 …

Genetic Resistance To Staphylococcus Aureus Mastitis Associated With Bovine Cxcr1, Olivia Jean Crisp

Chancellor’s Honors Program Projects

No abstract provided.

An Investigation Of Epigenetic Contributions To Inter-Animal And Age Dependent Variation In The Bovine Innate Immune Response., Benjamin Green

Graduate College Dissertations and Theses

Mastitis represents a major issue within the dairy industry responsible for economic loss via decreased animal productivity and associated veterinary costs. Currently, there is a push to identify a phenotypic innate immune response that will yield dairy cows with an enhanced resistance to mastitis. Bovine dermal fibroblasts were used as a cell model to measure the response of individuals to Gram-negative bacterial stimuli through the TLR4 signaling pathway. Fibroblast cultures were isolated from 15 dairy heifers at 5, 11, and 16 months of age in order to determine the variability in responsiveness to LPS as well as to monitor the …

Interaction Of Isoflavones And Endophyte-Infected Tall Fescue Seed Extract On Vasoactivity Of Bovine Mesenteric Vasculature, Yang Jia

Theses and Dissertations--Animal and Food Sciences

Endophyte-infected tall fescue produces many ergot alkaloids, which have been shown to be vasoconstrictive in various vessel types of bovine. On the other hand, substantial evidence has been reported on the vasodilative effects of formononetin and biochanin A in different vessel types in humans and rats. So, a study was conducted using mesenteric vasculature collected from heifers shortly after slaughter. After 2-h incubation with formononetin (F), biochanin A (B), or an ergovaline-containing tall fescue seed extract (EXT) and their combinations, vessels were mounted in a multi-myograph to determine their ergotamine-induced contractility. Results indicated that F and B at 1 × …

Regulation Of Oocyte Meiotic Resumption Using Camp Modulators In Bovine In Vitro Maturation, Sarah Farmer

LSU Master's Theses

In vitro maturation (IVM) is a reproductive technique critical to in vitro embryo production (IVP) in commercial livestock industries, research, and human infertility treatment. Currently, IVP has low efficiency due to an inadequate IVM system in which premature meiotic resumption results in low oocyte viability. Meiotic arrest is regulated primarily by 3’,5’-cyclic adenosine monophosphate (cAMP), and the most successful methods of improving IVM utilize cAMP modulators to maintain high intra-oocyte cAMP, delaying the onset of maturation. This thesis includes experiments comparing standard bovine IVM to a novel extended IVM method similar to the procedure described by Albuz and colleagues (Albuz …

Determination Of The Expression Patterns Of Bovine Non-Classical Major Histocompatibility Complex (Mhc) Class I Proteins, Parveen Parasar

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This project was funded by the United States of Department of Agriculture (USDA), which funds research aimed at improving production and animal health. The aim of this study was to advance knowledge of maternal immune tolerance to the fetus and mechanisms bovine non-classical MHC class I proteins employ to interact with immune cells and render them inert towards the fetus.

A fetus is a tissue graft inside the mother’s uterus yet must be accepted by the mother to maintain a successful pregnancy. Reproductive insufficiency and pregnancy failure are major causes of production loss in cattle, especially in cloned animals. Knowledge …

Bovine Mammary Interleukin-8 Receptor Expression And Genetic Association With Streptococcus Uberis Based Mastitis., Lydia Jean Siebert

Masters Theses

Mastitis is an economically distressing disease in the dairy industry. Bacterial pathogens enter the gland and encounter cell types that release immune mediators including interleukin (IL)-8. IL-8 has two membrane bound receptors: CXCR1 and CXCR2. CXCR1 and CXCR2 are expressed on neutrophils and other cell types in other species but their expression is unknown in the bovine mammary gland. To test this, mammary tissue samples from six Holstein dairy cows were subject to dual immunofluorescence with bovine specific CXCR1 and CXCR2 antibodies and cell type markers. CXCR1 was expressed on alveolar epithelial cells, fibroblasts, and leukocytes. CXCR2 expression was identified …