Life Sciences Commons^™

Characterization Of A Novel Stat 2 Knock Out Hamster Model Of Crimean Congo Hemorrhagic Fever Virus Pathogenesis, Charlene Ranadheera, Emelissa J. Valcourt, Bryce M. Warner, Guillaume Poliquin, Kyle Rosenke, Kathy Frost, Kevin Tierney, Greg Saturday, Jinxin Miao, Jonna B. Westover, Brian B. Gowen, Stephanie Booth, Heinz Feldmann, Zhongde Wang, David Safronetz

Animal, Dairy, and Veterinary Science Faculty Publications

Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne pathogen causing a febrile illness in humans, which can progress to hemorrhagic manifestations, multi-organ failure, and death. Current mouse models of CCHFV infection reliably succumb to virus challenge but vary in their ability to reflect signs of disease similar to humans. In this study, we established a signal transducer and activator of transcription 2 (STAT2) knockout hamster model to expand the repertoire of animal models of CCHFV pathogenesis that can be used for therapeutic development. These hamsters demonstrated a systemic and lethal disease in response to infection. Hallmarks of human …

Fetal Demise And Failed Antibody Therapy During Zika Virus Infection Of Pregnant Macaques, Diogo M. Magnani, Thomas F. Rogers, Nicholas J. Maness, Nathan D. Grubaugh, Nathan Beutler, Varian K. Bailey, Lucas Gonzalez-Nieto, Martin J. Gutman, Núria Pedreño-Lopez, Jaclyn M. Kwal, Michael J. Ricciardi, Tereance A. Myers, Justin G. Julander, Rudolf P. Bohm, Margaret H. Gilbert, Faith Schiro, Pyone P. Aye, Robert V. Blair, Mauricio A. Martins, Kathrine P. Falkenstien, Amitinder Kaur, Christine L. Curry, Esper G. Kallas, Ronald C. Desrosiers, Pascal J. Goldschmidt-Clermont, Stephen S. Whitehead, Kristian G. Andersen, Myrna C. Bonaldo, Andrew A. Lackner, Antonito T. Panganiban, Dennis R. Burton, David I. Watkins

Animal, Dairy, and Veterinary Science Faculty Publications

Zika virus (ZIKV) infection of pregnant women is associated with pathologic complications of fetal development. Here, we infect pregnant rhesus macaques (Macaca mulatta) with a minimally passaged ZIKV isolate from Rio de Janeiro, where a high rate of fetal development complications was observed. The infection of pregnant macaques with this virus results in maternal viremia, virus crossing into the amniotic fluid (AF), and in utero fetal deaths. We also treated three additional ZIKV-infected pregnant macaques with a cocktail of ZIKV-neutralizing human monoclonal antibodies (nmAbs) at peak viremia. While the nmAbs can be effective in clearing the virus from …

Small-Animal Models Of Zika Virus, Justin G. Julander, Venkatraman Siddharthan

Animal, Dairy, and Veterinary Science Faculty Publications

Zika virus (ZIKV) infection can result in serious consequences, including severe congenital manifestations, persistent infection in the testes and neurologic sequelae. After a pandemic emergence, the virus has spread to much of the new world and has been introduced to many countries outside of endemic areas as infected travelers return to their home countries. Rodent models have been important in gaining a better understanding of the wide range of disease etiologies associated with ZIKV infection and for the initial phase of developing countermeasures to prevent or treat viral infections. We discuss herein the advantages and disadvantages of small animal models …

Development Of A New Tacaribearenavirus Infection Model And Its Use To Explore Antiviral Activity Of A Novelaristeromycin Analog, Brian B. Gowen, M. H. Wong, D. Larson, W. Ye, K. H. Jung, E. J. Sefing, Ramona T. Skirpstunas, Donald F. Smee, John D. Morrey, S. W. Schneller

Animal, Dairy, and Veterinary Science Faculty Publications

Background

A growing number of arenaviruses can cause a devastating viral hemorrhagic fever (VHF) syndrome. They pose a public health threat as emerging viruses and because of their potential use as bioterror agents. All of the highly pathogenic New World arenaviruses (NWA) phylogenetically segregate into clade B and require maximum biosafety containment facilities for their study. Tacaribe virus (TCRV) is a nonpathogenic member of clade B that is closely related to the VHF arenaviruses at the amino acid level. Despite this relatedness, TCRV lacks the ability to antagonize the host interferon (IFN) response, which likely contributes to its inability to …

Antiviral Activities Andphosphorylation Of 5-Halo-2'-Deoxyuridines And N-Methanocarbathymidine In Cells Infectedwith Vaccinia Virus, Donald F. Smee, D. E. Humphreys, B. L. Hurst, Dale L. Barnard

Animal, Dairy, and Veterinary Science Faculty Publications

The Antipoxviral activities and phosphorylation of N-methanocarbathymidine ([N]-MCT) and four 5-halo-2'-deoxyuridines, namely 5-flouro- (FdU), 5-chloro- (CldU), 5-bromo- (BrdU), and 5-iodo- (IdU) derivatives were explored.

Cantagious Foot Rot Of Sheep, Clell V. Bagley

All Archived Publications

No abstract provided.

The Initiation Of Infection By Dna From Bacteriophage Lambda, Gerald D. Elseth

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Deoxyribonucleic acid isolated from bacteriophage λ can infect Escherichia coli K12 in the presence of adsorbed helper phage (Kaiser and Hogness, 1960; Kaiser, 1962). The manner in which λ DNA enters the cell and the possible role of helper phage in the penetration process is still not clear. Kinetic studies conducted in this laboratory during the initial stages of infection by λ DNA demonstrate a requirement for helper function during the penetration of an infectious molecule. Further investigation into this problem is needed and was the major objective of this study.