Life Sciences Commons^™

Comparing The Tadpoles Of Hyla Geographica And Hyla Semilineata, Anne D'Heursel, Rafael O. De Sá

Biology Faculty Publications

External morphology, internal oral anatomy, and chondrocranial anatomy were examined for tadpoles of Hyla geographica from the Amazon rainforest, Brazil, and Hyla semilineata from the Atlantic rainforest, Brazil. Here, we provide morphological larval data to help diagnose these closely related species. Scanning electron microscopy analysis of buccal morphology showed the most distinctive features between these species: the distance between the lingual papillae in the buccal floor of H. geographica is three times greater than that distance in H. semilineata, and the relative size of the lingual papillae in H. geographica is less than half their size in H. semilineata. Although …

Estructura Del Condrocráneo Y Esqueleto Visceral De Larvas De Pseudis Minuta (Anura, Pseudidae, E. O. Lavilla, Rafael O. De Sá

Biology Faculty Publications

The chondrocranium and visceral skeleton of Pseudis minuta tadpoles are described, based on a series of five larvae in stages 31 - 35 of Gosner (1960). Among their striking characters are the presence of peculiar articular surfaces between cornua trabeculae and suprarostral cartilage, the incomplete development of the orbital cartilage, the high fenestration of the floor of the cavum cranii, the fusion of posterior foramina, and the fusion of spicules 3 and 4 in the hyobranchial skeleton.

The Timing And Pattern Of Myogenesis In Hymenochirus Boettgeri, Matthew T. Smetanick, Rafael O. De Sá, Gary P. Radice

Biology Faculty Publications

Differences in the relative timing of homologous developmental events among closely related species, known as heterochronies, may provide valuable clues in understanding evolutionary relationships (McKinney, 1988; McNamara, 1995). Examining the timing of myogenic events is a relatively easy and effective method for finding heterochronic events. For example, whether muscle proteins and myofibrils appear before or after multinucleation can be determined through histological techniciques (Kielbowna, 1981). Simple observations of live specimens can pinpoint functional landmarks such as first twitch (spontaneous or due to external stimuli) and first heartbeat.

The Chondrocranium Of The Mexican Burrowing Toad, Rhinophrynus Dorsalis, Charles C. Swart, Rafael O. De Sá

Biology Faculty Publications

The chondrocranium of Rhinophrynus dorsalis is described and illustrated. Autapomorphies of R. dorsalis are the presence of the symplectic cartilage and a cartilaginous process on the ascending process of the palatoquadrate. Synapomorphies of Rhinophrynus shared with the Pipidae are: (1) early formation of the lower jaw and (2) presence of a suprarostral plate. Alternative developmental pathways for the formation of the suprarostral plate are considered. Histological data indicates that the suprarostral plate forms from: (1) the early fusion of the cornua trabeculae with the suprarostral cartilages, (2) an anterior growth of the ethmold plate, and (3) subsequent fusion …

Unidad De Los Anfibios Modernos Como Grupo Natural, Rafael O. De Sá

Biology Faculty Publications

RESUMEN

La monofilia de la infraclase Lissamphibia ha sido cuestionada. Algunos autores sugieren un origen independiente del Orden Gymnophiona basados en la condición compacta (= estegocrotafía) del cráneo de la cecilias. La presente revisión indica la irrelevancia del estegocrotafismo para establecer las relaciones filogenéticas del grupo. Los datos disponibles sugieren: 1) el estegocrotafismo es secundariamente derivado en cecilias, y 2) Lissamphibia representa un grupo monofilético.

SUMMARY

Unity of modern Amphibians as a natural group

The monophyly of Lissamphibia has been previously questioned. Some authors suggested an independent origin of Gymnophiona based on the solid condition (= stegokrotaphy) of the caecilian …

Chondrocranial Anatomy And Skeletogenesis In Dendrobates Auratus, Rafael O. De Sá, Shannon Hill

Biology Faculty Publications

The larval chondrocranium and visceral skeleton of Dendrobates auratus ls described and compared with those of other dendrobatids. Four characters, i.e. lack of fusion between orbital cartilages and otic capsules, wide processus muscularis palatoquadrati, lack of processus pseudopterygoideus, and lack of processus anterolateralis hyalis, represent derived conditions for Dendrobates within Dendrobatidae. Cranial and postcranial ossification sequences are reported for D. auratus and Epipedobates tricolor. Skeletogenesis is earlier in E. tricolor, but the overall pattern of ossification is similar in the two species.

Description Of Tadpoles Of Three Species Of Scinax (Anura: Hylidae), Rafael O. De Sá, Rocjard Wassersig, Arturo I. Kehr

Biology Faculty Publications

Larval morphological characteristics have been used to cluster species of Scinax in seven species groups and to support the monophyly of some of these groups. However, our present knowledge of Scinax tadpoles is incomplete; currently, tadpoles of only 21 of the approximately 80 recognized species have been described. Herein, we describe and illustrate the external morphology and oral disc characteristics of larval Scinax berthae, S. nebulosa, and S. boesemani. The presence of a "labial arm" supporting the P3 tooth row in S. boesemani, and its absence from S. nebulosa, is discussed in the light of our limited knowledge of tadpoles …

Description Of The Tadpole Of Atelopus Tricolor, E. O. Lavilla, Rafael O. De Sá, Ignacio De La Riva

Biology Faculty Publications

Tadpoles of Atelopus belong to the gastromyzophorous guild of exotrophic larvae (Altig and Johnston, 1989), and are characterized by the presence of a conspicuous abdominal sucker and an expanded oral disc. The larvae of several Atelopus species have been described previously: A. varius (Starrett, 1967); A. certus, A. ignescens, and A. spumarius (Duellman and Lynch, 1969); A. cruciger (Mebs, 1980); A. flavescens (Lescure, 1981); A. peruensis (Gray and Cannatella, 1985); and A. subornatus (Lynch, 1986). These represent about 15% of the known species in the genus (Frost, 1985; Duellman, 1993). Although Atelopus tricolor was originally described at the beginning of …

External Morphology Of The Chorion Of The Annual Fishes Cynolebias (Cyprinodontiformes: Rivulidae), Marcelo Loureiro, Rafael O. De Sá

Biology Faculty Publications

Members of the family Rivulidae (killifishes) inhabit temporary bodies of freshwater in South and Central America (one species is also found in North America). The most remarkable characteristic of the family Rivulidae is that species have an annual life cycle with a drought-resistant egg during the dry season. Parenti's (1981) analysis of the order Cyprinodontiformes considered a single genus, Cynolebias, whereas Cos- ta's (1990) phylogenetic analysis of the family Rivulidae separates Cynolebias from Cynopoecilus. One of Costa's synapomorphies to separate Cynopoecilus is the unique structure of their egg's chorion, which is shared with Leptolebias and Campellolebias.

Hyla Multifasciata, Rafael O. De Sá

Biology Faculty Publications

Hyla multifasciata is a medium to large species in the albopunctata species group, adults ranging in size from 42 - 61 mm SVL (mean male SVL = 48 mm, mean female SVL = 53 mm). The head is longer than wide. The snout is rounded in dorsal view, whereas in lateral view it is rounded and protrudes slightly beyond the mandible. The canthus rostralis is rounded and distinct, the loreal region is concave, and the internarial region is convex. The eyes are large, approximately twice the diameter of the tympanum. The tympanum is separated from the eye by a distance …

Leptodactylus Silvanimbus (Amphibia: Anura: Leptodactylidae): Natural History Notes, Advertisement Call, And Relationships, W. Ronald Heyer, Rafael O. De Sá, James R. Mccranie, Larry David Wilson

Biology Faculty Publications

In July 1995, we visited two of the three previously known localities for Leprodactylus silvanimbus in Departamento de Ocotepeque, Honduras. We found a new breeding site for the species, recorded its advertisement call, and collected and observed its adults and larvae. The purpose of this paper is to present the results of these new findings and to comment on the relationships L. silvanimbus.

Características De La Osificación Craneal En Phyllomedusa Boliviana (Anura: Hylidae), Rafael O. De Sá, E. O. Lavilla

Biology Faculty Publications

RESUMEN. Dentro de la familia Hylidae disponemos de información sobre las secuencias de osificación craneal para menos del 4.0% de sus miembros. El presente trabajo describe la secuencia de osificación craneal de Phyllomedusa boliviana. El patrón general de osificación es similar a lo reportado para otras especies de la familia, pero existen algunas diferencias importantes tales como la osificación tardía de los próoticos y la osificación premetamórfica de neopalatino, vómer, cuadrado-yugal, pterigoides y columela. El lento desarrollo ulterior de la osificación resulta en juveniles con cráneos extensamente cartilaginosos.

ABSTRACT. Characteristics of the cranial ossification in Phyllomedusa …

Hyla Albopunctata, Rafael O. De Sá

Biology Faculty Publications

Hyla albopunctata is a medium-sized species in the albopunctata group, adults range in size from 42-52 mm SVL (males average 47 mm and females 49 mm). The head is triangular in outline, always longer than wide. The snout is moderately long and acuminate in dorsal view, whereas in lateral view, it is acuminate and protrudes beyond the mandible to form an angle of 45 degrees over the mandible. The canthus rostralis is rounded and distinct, the loreal region is slightly concave, and the internarial region is slightly convex. The eyes are large, approximately twice the diameter of the tympanum. The …

Terrapene Carolina Carolina, Joseph C. Mitchell, Rafael O. De Sá

Biology Faculty Publications

TERRAPENE CAROLINA CAROLINA (Eastern Box Turtle). REPRODUCTION. Female Terrapene carolina containing oviductal eggs have been recorded in Virginia from 26 May to 25 July (Mitchell, in press. The Reptiles of Virginia. Smithsonian Inst. Press, Washington, D.C.). Other reports of reproduction in Terrapene (see list in Ernst and McBreen 1991. Cat. Amer. Amphib. Rept. 512.1-512.13) indicate that nesting occurs in late spring and early summer throughout its range.

Description Of The Tadpoles Of Gastrophryne Pictiventris And Nelsonophryne Aterrima (Anura: Microhylidae), With A Review Of Morphological Variation In Free-Swimming Microhylid Larvae, Maureen A. Donnelly, Rafael O. De Sá, Craig Guyer

Biology Faculty Publications

The tadpoles of Gastrophryne pictiventris and Nelsonophryne aterrima are described based on material collected in Costa Rica and Panama, respectively. The tadpole of G. pictiventris resembles those of the other species of Gastrophryne. The tadpole of N. aterrima differs from other New World microhylid tadpoles in location of the vent tube (medial), the spiracular opening (ventral to the gut), and eyes (dorsolateral). The unique configuration of mouthparts in N. aterrima is described. Morphological variation in microhylid tadpoles is reviewed and six larval characters are analyzed cladistically for IO New World microhylid genera.

Histology Of The Ovaries And Fat Bodies Of Chthonerpeton Indistinctum, Rafael O. De Sá, Nibia Berois

Biology Faculty Publications

Histological analysis revealed three different stages in the development of ovarian follicles of Chthonerpeton indistinctum. These stages are identified by (1) location of the oocyte in the ovary wall, (2) oocyte morphology and staining characteristics, (3) development of the zona pellucida, and (4) organization of the follicular layer. Atretic follicles and corpora lutea also were found. Fat bodies are composed of well-developed adipose tissue.

Morfología Y Funciones Del Conducto De Müller De Chthonerpeton Indistinctum (Reinhardt Y Lutken, 1862) (Amphibia: Gymnophiona) En Relación Con Los Mecanismos Reproductivos De La Especie, Nibia Berois, Rafael O. De Sá

Biology Faculty Publications

El orden Gymnophiona comprende anfibios ápodos, ciegos, de hábitos cavícolas y acuáticos. Como consecuencia de estos hábitos resulta muy difícil su colecta, lo cual redunda en un escaso conocimiento de la biología del orden y sobre las relaciones filogenéticas del mismo.

Una de las características más particulares del grupo se refiere a la modalidad reproductora, ya que aproximadamente el 50% de las especies presentan viviparidad y la totalidad de ellas tienen fecundación interna. Es por esta razón que el estudio del aparato reproductor ha sido encarado por diversos autores, en especial por Wake (1968, J. Morphol. 126: 291) con una …

Estructura Del Ovario De Chthonerpeton Indistinctum (Reinhardt Y Lutken, 1862) (Amphibia: Gymnophiona) Durante La Gestación, Rafael O. De Sá, Nibia Berois

Biology Faculty Publications

La morfología e histología del ovario para varias especies del orden Gymnophiona ha sido reportada por Wake (1968, J. Morphol. 126, 291) y para C. indistinctum por los autores (1984, XXIV Reunión de Comunicaciones, Soc. Zool. Uruguay).

Continuando con los estudios sobre el aparato genital de esta especie vivípara, se presenta ahora la estructura del ovario durante la gestación.

Se procesaron con fines histológicos, ovarios de dos hembras grávidas colectadas en las costas de los Dep. Montevideo y San José en abril, 1983. El número de ejemplares trabajados es muy reducido dado lo difícil que resulta su colecta …

Gametogenesis En Chthonerpeton Indistinctum (Gymnophiona: Typhlonectidae), Rafael O. De Sá, Nibia Berois

Biology Faculty Publications

RESUMEN:

Histológicamente, las gónadas de Chthonerpeton indistinctum son similares a las ya descritas para otras especies del orden Gymnophiona. Los lóbulos testiculares se hallan compartimentados en unidades estructurales denominadas lóculos. La espermatogénesis ocurre en cistos sincrónicos de células germinales. Durante la espermiogénesis se identifican cuatro estados consecutivos de desarrollo: 1) cistos de espermátidas redondeadas; 2) cistos de espermátidas arrifionadas; 3) cistos de espermátidas bastoniformes y 4) espermátidas en ramillete.

El análisis histológico de los ovarios mostró la presencia de tres estados en la maduración de los folículos ováricos. Estas etapas pueden ser identificadas de acuerdo a: 1) ubicación del folículo …

Leptodactylus Podicipinus (Cope, 1862) Y Leptodactylus Chaquensis Cei, 1959 (Anura, Leptodactylidae), En El Uruguay, Raúl Vaz-Ferreira, Rafael O. De Sá, Federico Achaval, Annelise Gebrau

Biology Faculty Publications

En base al estudio de las colecciones obtenidas principalmente en excursiones a la zona NO de Uruguay con motivo del relevamiento faunístico del área de Salto Grande, se avanzó considerablemente en el conocimiento de la distribución de Leptodactylus podicipinus (Cope, 1862) y Leptodactylus chaquensis (Cei, 1950), en nuestro país.

Se cita el material estudiado y las observaciones de campo realizadas; se dan los caracteres generales de ambas especies, se describen los hábitats, y se tratan las características de las vocalizaciones.

Observaciones Sobre La Biología De Phyllomedusa Iheringi Boulenger, 1885, (Anura, Hylidae), Rafael O. De Sá, Annelise Gerhau

Biology Faculty Publications

RESUMEN:

Se presentan las características que permiten diferenciar las larvas de Phyllomedusa iheringi de las de otros anuros que habitan en nuestro territorio, junto con algunas observaciones sobre la biología de esta especie poco conocida en el Uruguay.

SUMMARY:

The characteristics that differentiate the larvae of Phyllomedusa iheringi from other anurans that live in Uruguay are presented here. Some observations on the biology of this species are also recorded.