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Parthenogenetic Activation Of Bovine Oocytes Using Bovine And Murine Phospholipase C Zeta, Pablo J. Ross, Zeki Beyhan, Amy E. Iager, Sook Young Yoon, Christopher Malcuit, Karl Schellander, Rafael Fissore, Jose B. Cibelli
Parthenogenetic Activation Of Bovine Oocytes Using Bovine And Murine Phospholipase C Zeta, Pablo J. Ross, Zeki Beyhan, Amy E. Iager, Sook Young Yoon, Christopher Malcuit, Karl Schellander, Rafael Fissore, Jose B. Cibelli
Rafael Fissore
Background - During natural fertilization, sperm fusion with the oocyte induces long lasting intracellular calcium oscillations which in turn are responsible for oocyte activation. PLCZ1 has been identified as the factor that the sperm delivers into the egg to induce such a response. We tested the hypothesis that PLCZ1 cRNA injection can be used to activate bovine oocytes. Results - Mouse and bovine PLCZ1 cRNAs were injected into matured bovine oocytes at different concentrations. Within the concentrations tested, mouse PLCZ1 injection activated bovine oocytes at a maximum rate when the pipette concentration of cRNA ranged from 0.25 to 1 μg/μL, …