Life Sciences Commons^™

Regulation Of Cancer Stem Cells By P53, D. Joseph Jerry, Luwei Tao, Haoheng Yan

D. Joseph Jerry

The hypothesis that cancer stem cells are responsible for the chemoresistant and metastatic phenotypes of many breast cancers has gained support using cell-sorting strategies to enrich the tumor-initiating population of cells. The mechanisms regulating the cancer stem cell pool, however, are less clear. Two recent publications suggest that loss of p53 permits expansion of presumptive cancer stem cells in mouse mammary tumors and in human breast cell lines. These results add restriction of cancer stem cells as a new tumor suppressor activity attributed to p53.

Estrogen And Progesterone Induce Persistent Increases In P53-Dependent Apoptosis And Suppress Mammary Tumors In Balb/C-Trp53+/- Mice, D. Joseph Jerry, K.A. Dunphy, A.C. Blackburn, H. Yan, L.R. O'Connell

D. Joseph Jerry

Introduction - Treatment with estrogen and progesterone (E+P) mimics the protective effect of parity on mammary tumors in rodents and depends upon the activity of p53. The following experiments tested whether exogenous E+P primes p53 to be more responsive to DNA damage and whether these pathways confer resistance to mammary tumors in a mouse model of Li-Fraumeni syndrome. Methods Mice that differ in p53 status (Trp53+/+, Trp53+/-, Trp53-/-) were treated with E+P for 14 days and then were tested for p53-dependent responses to ionizing radiation. Responses were also examined in parous and age-matched virgins. The effects of hormonal exposures on …

Parthenogenetic Activation Of Bovine Oocytes Using Bovine And Murine Phospholipase C Zeta, Pablo J. Ross, Zeki Beyhan, Amy E. Iager, Sook Young Yoon, Christopher Malcuit, Karl Schellander, Rafael Fissore, Jose B. Cibelli

Rafael Fissore

Background - During natural fertilization, sperm fusion with the oocyte induces long lasting intracellular calcium oscillations which in turn are responsible for oocyte activation. PLCZ1 has been identified as the factor that the sperm delivers into the egg to induce such a response. We tested the hypothesis that PLCZ1 cRNA injection can be used to activate bovine oocytes. Results - Mouse and bovine PLCZ1 cRNAs were injected into matured bovine oocytes at different concentrations. Within the concentrations tested, mouse PLCZ1 injection activated bovine oocytes at a maximum rate when the pipette concentration of cRNA ranged from 0.25 to 1 μg/μL, …

Protein-Passivated Fe3o4 Nanoparticles: Low Toxicity And Rapid Heating For Thermal Therapy, D. Joseph Jerry, Bappaditya Samanta, Haoheng Yan, Nicholas O. Fisher, Jing Shi, Vincent M. Rotello

D. Joseph Jerry

Thermotherapy is a promising technique for the minimally invasive elimination of solid tumors. Here we report the fabrication of protein -coated iron oxide NPs (12 nm core) for use as thermal therapeutic agents. These albumin-passivated NPs are stable under physiological conditions, with rapid heating and cell killing capacity upon alternating magnetic field (AMF) exposure. The mode of action is specific: no measurable cytotoxicity was observed for the particle without AMF or for AMF exposure without the particle.

Holyoke Dam, Connecticut River, 2008, Brett Towler

Fish Passage Data Archive

Holyoke Dam

Connecticut River

Massachusetts

Year data collected: 2008

Potential Of Using Breast Milk As A Tool To Study Breast Cancer And Breast Cancer Risk, Kathleen Arcaro, D.L. Anderton

Kathleen Arcaro

No abstract provided.