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Angiotensin I-Converting Enzyme Inhibitory And Antioxidative Activities Of The Bioactive Peptides In The Crude Salt-Soluble Protein Hydrolysates Of Pili Nut (Canarium Ovatum Engl.) Cv. Mayon Kernel, Ella Mae Gamba, Lawrence Yves Uy, Mary Ann Torio
Angiotensin I-Converting Enzyme Inhibitory And Antioxidative Activities Of The Bioactive Peptides In The Crude Salt-Soluble Protein Hydrolysates Of Pili Nut (Canarium Ovatum Engl.) Cv. Mayon Kernel, Ella Mae Gamba, Lawrence Yves Uy, Mary Ann Torio
The Philippine Agricultural Scientist
The in silico enzyme digestion and peptide function annotation of pili nut (Canarium ovatum Engl.) 11S globulin protein sequence revealed the presence of bioactive peptides with antihypertensive and antioxidative activities. Molecular docking simulations showed that the proline-histidine (PH) ligand can better inhibit angiotensin I-converting enzyme (ACE) activity than captopril. This result was verified through biochemical assays. Pili kernel protein was extracted with 0.5 M NaCl buffer using the modified Osborne fractionation method, which yielded 100 mg of protein per gram of defatted pili kernel determined via Bradford assay. SDS-PAGE suggested that the protein extract had a major legumin-like (11S) …