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2010

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Articles 211 - 240 of 336

Full-Text Articles in Operations Research, Systems Engineering and Industrial Engineering

Design And Development Of An Integrated Waste Management System For Huai Nam Khun, Thailand, Annie Duong Jan 2010

Design And Development Of An Integrated Waste Management System For Huai Nam Khun, Thailand, Annie Duong

Industrial and Manufacturing Engineering

Background

A rural village in Thailand has always been limited in access to waste collection services and processing facilities. As a result, their behaviors of disposing waste in ineffective manners such as dumping and burning causes a variety of environmental impacts. In addition, the people dwelling in these areas are often unaware of the health risks associated to these types of engagements because of the inadequate education system. For a long time coming these circumstances remained untouched until a non-profit organization recently shed light to the need for a solution. An investigation, to develop a design solution in order to …


T.K. Ripper: A Multidisciplinary Group Project, Logan Hunt Jan 2010

T.K. Ripper: A Multidisciplinary Group Project, Logan Hunt

Industrial and Manufacturing Engineering

The purpose of this project is to provide solutions to objectives that require an industrial engineering (IE) background for a multidisciplinary group senior project. The group is creating a portable winch pulley, dubbed the T.K. Ripper, which will be used for extreme water sports. The group is interested in creating a business using this product, and has forecasted demand for the next three years. The objectives that were assigned from the group were a flow process chart, the cost of producing the product themselves in their own facility, the cost of producing the product if outsourced to China, how many …


Environmental Savings Tracking System For Cisco Systems, Inc., Lauren J. Herrera Jan 2010

Environmental Savings Tracking System For Cisco Systems, Inc., Lauren J. Herrera

Industrial and Manufacturing Engineering

This paper illustrates the research, methodology and design used to create an environmental savings tracking system, also known as the Green Dashboard, for Cisco Systems Inc.’s Green Initiatives Group to quantify and capture an annualized summary of savings from projects initiated within its Customer Value Chain Management Organization. It articulately describes the

methodology behind the selection of metrics and the design of the process required to collect and track these quantitative direct savings. As well as describes the benefits associated with implementing this system, such as shifting Cisco towards more sustainable products and increased customer satisfaction and investor relations by …


Small Business And Industrial Engineering Tools, Adib Behjat Jan 2010

Small Business And Industrial Engineering Tools, Adib Behjat

Industrial and Manufacturing Engineering

UltraMed, a small distribution company located in the United Arab Emirates, faces high operations cost due to inefficiency in methods and concepts implemented in their business. Through the use of basic Industrial Engineering techniques, UltraMed and other small businesses can succeed in reducing their operational costs through the implementation of three major concepts of Industrial Engineering: Operations Research, Database Management, and Engineering Economic Modeling.

To succeed in implementing the design, it is important to have the right data and a cohesive foundation for the design; else, the results generated by the techniques will be invalid and could cause further complications …


Layout And Procedure Re-Design Of Dcpp Receiving Warehouse, Nicole Elizabeth Riegel Jan 2010

Layout And Procedure Re-Design Of Dcpp Receiving Warehouse, Nicole Elizabeth Riegel

Industrial and Manufacturing Engineering

The receiving warehouse at Diablo Canyon Power Plant, owned by Pacific Gas and Electric, serves as a place to recognize the receiving of items into an ERP system, repackage and label items for long-term storage, and organize items for delivery inside the secured area. The warehouse managers would like to redesign the layout and procedures of the warehouse. Currently items are not received, scanned, or shipped in a timely manner. The flow of people and items in the receiving and shipping area is chaotic and space is limited especially during plant outages. The objective of this project is to: • …


Ernie Ball Guitars Woodshop Facilities Redesign, Cameron Lea Schlemer Jan 2010

Ernie Ball Guitars Woodshop Facilities Redesign, Cameron Lea Schlemer

Industrial and Manufacturing Engineering

This report details a facilities design for Ernie Ball’s guitar and bass guitar manufacturing facility in San Luis Obispo, CA. Specifically, this project will redesign the layout for the Woodshop, the largest of the 5 manufacturing cells in the facility. This project was completed using the Systematic Layout Planning procedure and focused mostly on phase II and III in which the specifics for a layout are determined, however, space requirements were not evaluated as it was not within scope.

The first step was to develop departments in the facilities; two master departments were identified for the neck manufacturing process and …


Non-Profit Management System For Friends Of The Elephant Seal, Matthew Justus, Robert Macasi, Jeff Zinn Jan 2010

Non-Profit Management System For Friends Of The Elephant Seal, Matthew Justus, Robert Macasi, Jeff Zinn

Industrial and Manufacturing Engineering

Friends of the Elephant Seal (FES) are a non-profit organization based in San Simeon, California. Their current method of tracking and reporting information is inefficient. A project was proposed to develop a new information management system for FES, install it at their office and provide training including a user manual. The project was divided into three parts: Database Design and Implementation; User Interface Design and Ergonomics; and User Manual & Help System.

The objective of the project was to design a user-friendly interface specifically for the FES worker that allowed for easy access to data entry functions, reporting functions and …


5-S And The Facility Redesign For A Cleaning Products Manufacturing Company, Jonathan Eipper Jan 2010

5-S And The Facility Redesign For A Cleaning Products Manufacturing Company, Jonathan Eipper

Industrial and Manufacturing Engineering

This project deals with a “green” cleaning products company in Southern California. Business has increased 38% in the past year along with changes in production capacity and capability, so the company has identified a need for a sustainable model of manufacturing at their new and expanded facility. Goals of promoting sustainable waste management, minimizing types of lean waste, properly storing inventory, improving workplace communication, and defining production tasks will be addressed in this facilities redesign through 5S. The biggest component and outcome of the facility design will be organization. This leads to a goal of eliminating waste, which will in …


Alleviation Of Spindle Transfer, Blake Ochs, Whitney Bone Jan 2010

Alleviation Of Spindle Transfer, Blake Ochs, Whitney Bone

Industrial and Manufacturing Engineering

Haas Automation, Inc. is one of the largest CNC machine tool builders in the world. They produce an assortment of machines that come in many different sizes, however, all machines have in common the central tool that allows them to perform their surfacing and bore functions, the spindle. As with the machines themselves, the spindles can also range in size depending on the type of machine they are going into. These spindles are assembled step by step and as components are added to them, several models become so heavy that it is not feasible to have operators lift them by …


Design For Manufacturing Of Sports Equipment, Cedrick Bit, Christopher L. Head Jan 2010

Design For Manufacturing Of Sports Equipment, Cedrick Bit, Christopher L. Head

Industrial and Manufacturing Engineering

The purpose of this project was to validate industrial designs for a product in its conceptual stage and suggest/implement design changes that suit the contracting company’s needs while assuring the product maintains its basic functional requirements. Techniques and methods used to validate the design include 3‐D solid modeling, physical product application using rapid prototyping to produce the product. Methods of design for manufacturing were analyzed in the design. Processes used in the validation and verification of the design include solid modeling design, methods of measurements, fixture design and manufacturing processes. Full design specifications and solid model files were finalized and …


Digester Selection And Operation For Heybeli Island, Spencer Fackler, Joseph Violetti Jan 2010

Digester Selection And Operation For Heybeli Island, Spencer Fackler, Joseph Violetti

Industrial and Manufacturing Engineering

The Heybeli Digester project originated with Sema Alptekin in her personal research and was incorporated into a Senior Project opportunity at California Polytechnic State University, San Luis Obispo. The aim of this project was to select and design a plan for the use of a digester for the island of Heybeli, Turkey. The objective of this plan was to incorporate economic, social, and environmental justifications to evaluate if a digester would be a benefit to the community.

Systems Engineering analysis is a vital tool when working with complicated projects that include multiple systems interfacing with one another. Through the use …


Determining A Reliability Factor For Photovoltaic's In San Luis Obispo, Ryan S. Roach Jan 2010

Determining A Reliability Factor For Photovoltaic's In San Luis Obispo, Ryan S. Roach

Industrial and Manufacturing Engineering

This project will analyze raw photovoltaic (PV) solar panel performance data in order to determine a reliability factor for solar panels in San Luis Obispo. This project is a subset of a larger project being performed by SLO RESCO, which is funded by a grant from the California Energy Commission. Their project will explore the use of solar, wind, geothermal, and biomass renewable resources. The scope of this project is limited to a small section of RESCO's analysis of solar panel technology and implementation in which a reliability factor will be generated.

The objective is to determine a reliability factor …


Application Of Radio Frequency Identification To An Asset Checkout System, Ma'ayan K. Bennaim Jan 2010

Application Of Radio Frequency Identification To An Asset Checkout System, Ma'ayan K. Bennaim

Industrial and Manufacturing Engineering

Radio Frequency Identification (RFID) is a growing technology which is increasingly utilized throughout industry. This senior project identifies an RFID alternative to the checkout system in the tool crib of the Cal Poly Aero Hanger. A new database is designed and built to interface with an RFIDeas AIR enroll reader. The system realizes vast time savings in check in times and increases student accountability for the tools which are checked out by tagging tools.


The Optimal Warehouse Location For Cal Poly's Campus Dining, Robert Buie Jan 2010

The Optimal Warehouse Location For Cal Poly's Campus Dining, Robert Buie

Industrial and Manufacturing Engineering

The Cal Poly Corporation and Campus Dining face the challenge of meeting the growth of students and dining venues on campus with the limited space capacity of their warehouse in building 19. Given a $250,000 budget, Campus Dining has an option to keep the warehouse at the current location at building 19 or moving the warehouse to building 82 off Mt. Bishop Road. Each warehouse location has its benefits as building 19 is in the center of campus and building 82 has much more shelving space than building 19. The objective of this project was to select the better choice …


Visual Representations Of Queried Database Results Using Google's Mapping Technologies, Steven Michael Kilbert Jan 2010

Visual Representations Of Queried Database Results Using Google's Mapping Technologies, Steven Michael Kilbert

Industrial and Manufacturing Engineering

Database queries have been used in business applications throughout the world to filter out specific information which is often used as a basis for making a decision. Despite the broad use of queries, there are only a limited number of ways to represent results, the most common being a dynaset, or results table. This paper describes the investigation of methods to create more visual representations of results using maps to show queried geospatial data. From these methods, applications were successfully developed to create customized maps in Google Earth and Google Maps based on queried geographical data from Microsoft Access databases. …


Impact Of Woven Fabric: Experiments And Mesostructure-Based Continuum-Level Simulations, Ethan M. Parsons, Tusit Weerasooriya, Sai Sarva, Simona Socrate Jan 2010

Impact Of Woven Fabric: Experiments And Mesostructure-Based Continuum-Level Simulations, Ethan M. Parsons, Tusit Weerasooriya, Sai Sarva, Simona Socrate

United States Army: Publications

Woven fabric is an increasingly important component of many defense and commercial systems, including deployable structures, restraint systems, numerous forms of protective armor, and a variety of structural applications where it serves as the reinforcement phase of composite materials. With the prevalence of these systems and the desire to explore new applications, acomprehensive, computationally efficient model for the deformation of woven fabrics is needed. However, modeling woven fabrics is difficult due, inparticular, to the need to simulate the response both at the scale of the entire fabric and at the meso-level, the scale of the yarns that compose the weave. …


Chemisorption Of A Thiol-Functionalized Ruthenium Dye On Zinc Oxide Nanoparticles: Implications For Dye-Sensitized Solar Cells, Jagdeep Singh, Jisun Im, James E. Whitten, Jason W. Soares, Diane M. Steeves Jan 2010

Chemisorption Of A Thiol-Functionalized Ruthenium Dye On Zinc Oxide Nanoparticles: Implications For Dye-Sensitized Solar Cells, Jagdeep Singh, Jisun Im, James E. Whitten, Jason W. Soares, Diane M. Steeves

United States Army: Publications

ZnO is an alternative to TiO2-based dye-sensitized solar cells (DSSCs). Adsorption of cis-rutheniumbis [2,2'-bipyridine]-bis[4-thiopyridine] onto ZnO nanorods has been studied using X-ray and ultraviolet photoelectron spectroscopies (XPS and UPS). XPS indicates chemisorption with a surface density of ca. 1 × 1015 molecules/cm2, confirming the possibility of using thiol-terminated dyes for ZnO-based DSSC devices. The energy level diagram, based on UPS and absorbance spectroscopy, indicates that the LUMO of this dye is lower in energy than the ZnO conduction band edge, providing minimal enthalpic driving force for photovoltaic electron injection. However, optimization of thiol-functionalized Ru …


Practical Simulation Application: Evaluation Of Process Control Parameters In Twisted-Pair Cables Manufacturing System, Amr Mahfouz, Amr Arisha Jan 2010

Practical Simulation Application: Evaluation Of Process Control Parameters In Twisted-Pair Cables Manufacturing System, Amr Mahfouz, Amr Arisha

Articles

Intensive competition and rapid technology development of Twisted-Pair Cables (TPC) industry have left no room for competing manufacturers to harbour system inefficiencies. TPC are used in various communication and networks hardware applications; their manufacturing facilities face many challenges including various product configurations with different equipment settings, different product flows and Work in Process (WIP) space limitations. The quest for internal efficiency and external effectiveness forces companies to align their internal settings and resources with external requirements/orders, or in different words, significant factors must be set appropriately and identified prior to manufacturing processes. Integrated definition models (IDEF0, IDEF3) in conjunction with …


Controllability And Observability Of A Large Scale Thermodynamical System Via Connectability Approach, Virdiansyah Permana Jan 2010

Controllability And Observability Of A Large Scale Thermodynamical System Via Connectability Approach, Virdiansyah Permana

Electronic Theses and Dissertations

This study presents a new approach to determine the controllability and observability of a large scale nonlinear dynamic thermal system using graph-theory. The novelty of this method is in adapting graph theory for nonlinear class and establishing a graphic condition that describes the necessary and sufficient terms for a nonlinear class system to be controllable and observable, which equivalents to the analytical method of Lie algebra rank condition. The graph theory of a directed graph (digraph) is utilized to model the system, and the rule of its adaptation in nonlinear class is defined. Subsequently, necessary and sufficient terms to achieve …


The Analysis Of Rush Orders Risk In Supply Chain: A Simulation Approach, Amr Arisha, Amr Mahfouz Jan 2010

The Analysis Of Rush Orders Risk In Supply Chain: A Simulation Approach, Amr Arisha, Amr Mahfouz

Conference papers

Satisfying customers by delivering demands at agreed time, with competitive prices, and in satisfactory quality level are crucial requirements for supply chain survival. Incident of risks in supply chain often causes sudden disruptions in the processes and consequently leads to customers losing their trust in a company’s competence. Rush orders are considered to be one of the main types of supply chain risks due to their negative impact on the overall performance. Using integrated definition modeling approaches (i.e. IDEF0 & IDEF3) and simulation modeling technique , a comprehensive integrated model has been developed to assess rush order risks and examine …


Geochemical Investigations Of Metals Release From Submerged Coal Fly Ash Using Extended Elutriate Tests, A. J. Bednar, M. A. Chappell, J. M. Seiter, J. K. Stanley, D. E. Averett, W. T. Jones, B. A. Pettway, A. J. Kennedy, S. H. Hendrix, J. A. Steevens Jan 2010

Geochemical Investigations Of Metals Release From Submerged Coal Fly Ash Using Extended Elutriate Tests, A. J. Bednar, M. A. Chappell, J. M. Seiter, J. K. Stanley, D. E. Averett, W. T. Jones, B. A. Pettway, A. J. Kennedy, S. H. Hendrix, J. A. Steevens

United States Army: Publications

A storage pond dike failure occurred at the Tennessee Valley Authority Kingston Fossil Plant that resulted in the release of over 3.8 million cubic meters (5 million cubic yards) of fly ash. Approximately half of this material deposited in the main channel of the Emory River, 3.5 km upstream of the confluence of the Emory and Clinch Rivers, Tennessee, USA. Remediation efforts to date have focused on targeted removal of material from the channel through hydraulic dredging, as well as mechanical excavation in some areas. The agitation of the submerged fly ash during hydraulic dredging introduces river water into the …


Study Of Strawberry Flavored Milk Under Pulsed Electric Field Processing, Daniela Bermúdez-Aguirre, Jaime A. Yáñez, C. Patrick Dunne, Neal M. Davies, Gustavo V. Barbosa-Cánovas Jan 2010

Study Of Strawberry Flavored Milk Under Pulsed Electric Field Processing, Daniela Bermúdez-Aguirre, Jaime A. Yáñez, C. Patrick Dunne, Neal M. Davies, Gustavo V. Barbosa-Cánovas

United States Army: Publications

Few studies exist on flavored milk processed by pulsed electric fields (PEF). The main concern is product stability. This study aimed to analyze the degradation of coloring agent Allura Red in strawberry milk under PEF. Four systems were tested containing Allura Red: two commercial milks and two model systems. PEF conditions were 40 kV/cm, 48 pulses (2.5 μs), and 55 °C; coloring agent was quantified via RP-HPLC. After processing, only minor changes were observed in color, Allura Red concentration, and pH. During storage (32 d) at refrigerated conditions (4 °C) commercial samples maintained pH above 6. Model systems dropped below …


Nontuberculous Mycobacterium Infection In A Burn Icu Patient, Joseph M. Boyer, Peter J. Blatz, Kevin S. Akers, Jason F. Okulicz, Kevin K. Chung, Evan M. Rentz, Duane R. Hospenthal, Clinton K. Murray Jan 2010

Nontuberculous Mycobacterium Infection In A Burn Icu Patient, Joseph M. Boyer, Peter J. Blatz, Kevin S. Akers, Jason F. Okulicz, Kevin K. Chung, Evan M. Rentz, Duane R. Hospenthal, Clinton K. Murray

United States Army: Publications

Infection is a leading cause of mortality in burn patients, typically due to bacterial pathogens such as Staphylococcus aureus and Pseudomonas aeruginosa, less commonly fungi, and possibly viruses. In non-burn patients with underlying pulmonary or cutaneous disease, nontuberculous mycobacteria (NTM) have become an increasingly recognized cause of infection, especially in patients who are immunocompromised. Patients with severe burns might have higher rates of NTM infections due to inherent risks associated with the burn injury: compromised skin integrity, immunocompromised state, inhalation injury, and frequent use of indwelling vascular catheters. To date there have been no reports describing the incidence of …


Acute Caffeine Consumption Enhances The Executive Control Of Visual Attention In Habitual Consumers, Tad T. Brunyé, Caroline R. Mahoney, Harris R. Lieberman, Grace E. Giles, Holly A. Taylor Jan 2010

Acute Caffeine Consumption Enhances The Executive Control Of Visual Attention In Habitual Consumers, Tad T. Brunyé, Caroline R. Mahoney, Harris R. Lieberman, Grace E. Giles, Holly A. Taylor

United States Army: Publications

Recent work suggests that a dose of 200–400 mg caffeine can enhance both vigilance and the executive control of visual attention in individuals with low caffeine consumption profiles. The present study seeks to determine whether individuals with relatively high caffeine consumption profiles would show similar advantages. To this end, we examined the effects of four caffeine doses (0 mg, 100 mg, 200 mg, 400 mg) on low- and high-level visual attention in individuals with high consumption profiles (n = 36), in a double-blind study using a repeated measures design. Results from the Attention Network Test indicated that caffeine enhanced both …


Genomics And Proteomics In Chemical Warfare Agent Research: Recent Studies And Future Applications, Patrick A. Everly, James F. Dillman Jan 2010

Genomics And Proteomics In Chemical Warfare Agent Research: Recent Studies And Future Applications, Patrick A. Everly, James F. Dillman

United States Army: Publications

Medical research on the effects of chemical warfare agents (CWAs) has been ongoing for nearly 100 years, yet these agents continue to pose a serious threat to deployed military forces and civilian populations. CWAs are extremely toxic, relatively inexpensive, and easy to produce, making them a legitimate weapon of choice for terrorist organizations. While the mechanisms of action for manyCWAshave been known for years, questions about their molecular effects following acute and chronic exposure remain largely unanswered. Global approaches that can pinpoint which cellular pathways are altered in response to CWAs and characterize long-term toxicity have not been widely used. …


The Effects Of Bleedarrest, Celox, And Traumadex On Hemorrhage Control In A Porcine Model, Brian Gegel, James Burgert, Brian Cooley, Jacob Macgregor, Jules Myers, Sean Calder, Ralph Luellen, Michael Loughren, Don Johnson Jan 2010

The Effects Of Bleedarrest, Celox, And Traumadex On Hemorrhage Control In A Porcine Model, Brian Gegel, James Burgert, Brian Cooley, Jacob Macgregor, Jules Myers, Sean Calder, Ralph Luellen, Michael Loughren, Don Johnson

United States Army: Publications

Background

Hemorrhage is the second leading cause of death in civilian trauma and the leading cause of preventable death in military trauma. The purpose
of this study was to examine the effectiveness of three hemostatic agents: BleedArrest, TraumaDex, and Celox.

Materials and Methods

This was a prospective, experimental study using male Yorkshire swine. The pigs (n = 5 per group) were randomly assigned to one of the following: BleedArrest, TraumaDex, Celox, or control. To simulate a trauma injury, the investigators generated a complex groin injury with transection of the femoral artery and vein in all pigs. After 1 min of …


Effects Of The Heet Garment In The Prevention Of Hypothermia In A Porcine Model, Don Johnson, Brian Gegel, James Burgert, Geoffrey W, Duncklee, Ricci R. Robinson, Eric J. Lewis, Paul M. Crum, William Kuhns, Daniel Moore, Scott O'Brien, Joel Elliott, Jason Washington, John Boyle, Dale Seigler Jan 2010

Effects Of The Heet Garment In The Prevention Of Hypothermia In A Porcine Model, Don Johnson, Brian Gegel, James Burgert, Geoffrey W, Duncklee, Ricci R. Robinson, Eric J. Lewis, Paul M. Crum, William Kuhns, Daniel Moore, Scott O'Brien, Joel Elliott, Jason Washington, John Boyle, Dale Seigler

United States Army: Publications

Background

Hypothermia is a common battlefield trauma occurrence. This study compared the effectiveness of the hypothermia, environmental, exposure, and trauma (HEET) garment (Trident Industries, Beaufort, SC) with and without thermal inserts with a control group of two wool blankets in the prevention of hypothermia in a treated hypovolemic porcine model.

Materials and methods

Five female swine (Sus scrofa-Yorkshire cross) were assigned to each of three
groups: HEET with thermal inserts (n=5); HEET without thermal inserts (n=5); or control (n=5). After the animals were anesthetized and stabilized for 30min, the swine were hemorrhaged to a mean arterial pressure (MAP) of 30mm …


Morphology Control Of Tungsten Nanorods Grown By Glancing Angle Rf Magnetron Sputtering Under Variable Argon Pressure And Flow Rate, Khedir R. Khedir, Ganesh K. Kannarpady, Hidetaka Ishihara, Justin Woo, Charles Ryerson, Alexandru S. Biris Jan 2010

Morphology Control Of Tungsten Nanorods Grown By Glancing Angle Rf Magnetron Sputtering Under Variable Argon Pressure And Flow Rate, Khedir R. Khedir, Ganesh K. Kannarpady, Hidetaka Ishihara, Justin Woo, Charles Ryerson, Alexandru S. Biris

United States Army: Publications

Morphologically novel tungsten nanorods (WNRs) with the co-existence of two crystalline phases, α-W (thermodynamically stable) and β-W, were fabricated by glancing angle RF magnetron sputtering technique under various Ar pressures and flow rates. For these nanorods, a significant variation in their morphology and surface roughness was observed. These structures could be useful in a wide range of applications such as field emission, robust superhydrophobic coatings, energy, and medicine.


Inflammatory Effects Of Inhaled Sulfur Mustard In Rat Lung, Rama Malaviya, Vasanthi R. Sunil, Jessica Cervelli, Dana R. Anderson, Wesley W. Holmes, Michele L. Conti, Ronald E. Gordon, Jeffrey D. Laskin, Debra L. Laskin Jan 2010

Inflammatory Effects Of Inhaled Sulfur Mustard In Rat Lung, Rama Malaviya, Vasanthi R. Sunil, Jessica Cervelli, Dana R. Anderson, Wesley W. Holmes, Michele L. Conti, Ronald E. Gordon, Jeffrey D. Laskin, Debra L. Laskin

United States Army: Publications

Inhalation of sulfur mustard (SM), a bifunctional alkylating agent that causes severe lung damage, is a significant threat to both military and civilian populations. The mechanisms mediating its cytotoxic effects are unknown and were investigated in the present studies. Male rats Crl:CD(SD) were anesthetized, and then intratracheally intubated and exposed to 0.7–1.4 mg/kg SM by vapor inhalation. Animals were euthanized 6, 24, 48 h or 7 days post-exposure and bronchoalveolar lavage fluid (BAL) and lung tissue collected. Exposure of rats to SM resulted in rapid pulmonary toxicity, including focal ulceration and detachment of the trachea and bronchial epithelia from underlying …


Inhibition Of Hiv-1 Infection Of Peripheral Blood Mononuclear Cells By A Monoclonal Antibody That Binds To Phosphoinositides And Induces Secretion Of Β-Chemokines, Gary R. Matyas, Lindsay Wieczorek, Divya Bensal, Agnes-Laurence Chenine, Eric Saunders-Buell, Sodsai Tovanabutra, Jerome H. Kim, Victoria Polonis, Carl R. Alving Jan 2010

Inhibition Of Hiv-1 Infection Of Peripheral Blood Mononuclear Cells By A Monoclonal Antibody That Binds To Phosphoinositides And Induces Secretion Of Β-Chemokines, Gary R. Matyas, Lindsay Wieczorek, Divya Bensal, Agnes-Laurence Chenine, Eric Saunders-Buell, Sodsai Tovanabutra, Jerome H. Kim, Victoria Polonis, Carl R. Alving

United States Army: Publications

A murine IgG mAb, WR321, selected for the ability to bind to phosphatidylinositol-4-phosphate and phosphatidylinositol-4, 5-bisphosphate, but an inability to bind to any of 17 other lipids, including phosphatidylinositol, was examined as a probe for studying interactions of HIV-1 with primary human peripheral blood mononuclear cells. The WR321 mAb broadly neutralized CCR5-tropic strains of HIV-1 to prevent infection of the cells. The mAb also exhibited direct interaction with cells in the culture, resulting in secretion of chemokines that interfered with the interaction of HIV-1 virions with CCR5, the coreceptor for HIV-1 on the susceptible cells, leading to inhibition of infection …