Environmental Engineering Commons^™

Modeling Streambank Erosion On Composite Streambanks On A Watershed Scale, A. R. Mittelstet, D. E. Storm, G. A. Fox, P. M. Allen

Biological Systems Engineering: Papers and Publications

Streambanks can be a significant source of sediment and phosphorus to aquatic ecosystems. Although the streambank-erosion routine in the Soil and Water Assessment Tool (SWAT) has improved in recent versions, the recently developed routine in SWAT 2012 has undergone limited testing, and the lack of site or watershed specific streambank data increases the uncertainty in the streambank-erosion predictions. There were two primary objectives of this research: (1) modify and test the 2012 SWAT streambank-erosion routine on composite streambanks, and (2) compare SWAT default and field-measured channel parameters and assess their influence on predicted streambank erosion. Three modifications were made to …

Quantifying An Aquifer Nitrate Budget And Future Nitrate Discharge Using Field Data From Streambeds And Well Nests, Troy E. Gilmore, David P. Genereux, Kathleen M. Farrell, Helena Mitasova

Biological Systems Engineering: Papers and Publications

Novel groundwater sampling (age, flux, and nitrate) carried out beneath a streambed and in wells was used to estimate (1) the current rate of change of nitrate storage, dS_NO3 /dt, in a contaminated unconfined aquifer, and (2) future [NO₃^–]_FWM (the flow-weighted mean nitrate concentration in groundwater discharge) and f_NO3 (the nitrate flux from aquifer to stream). Estimates of dS_NO3 /dt suggested that at the time of sampling (2013) the nitrate storage in the aquifer was decreasing at an annual rate (mean = –9 mmol/m²yr) equal …

Effect Of Rainfall Timing And Tillage On The Transport Of Steroidhormones In Runoff From Manure Amended Row Crop Fields, Sagor Biswas, William L. Kranz, Charles A. Shapiro, Daniel D. Snow, Shannon L. Bartelt-Hunt, Mitiku Mamo, David D. Tarkalson, Tian C. Zhang, David P. Shelton, Simon J. Van Donk, Terry L. Mader

Biological Systems Engineering: Papers and Publications

Runoff generated from livestock manure amended row crop fields is one of the major pathways of hormone transport to the aquatic environment. The study determined the effects of manure handling, tillage methods, and rainfall timing on the occurrence and transport of steroid hormones in runoff from the row crop field. Stockpiled and composted manure from hormone treated and untreated animals were applied to test plots and subjected to two rainfall simulation events 30 days apart. During the two rainfall simulation events, detection of any steroid hormone or metabolites was identified in 8–86% of runoff samples from any tillage and manure …

Wetlands And Coastal Systems: Protecting And Restoring Valuable Ecosystems, C. T. Agouridis, K. R. Douglas-Mankin, A. C. Linhoss, A. R. Mittelstet

Biological Systems Engineering: Papers and Publications

Wetlands and coastal systems are unique, highly productive, and often threatened landscapes that provide a host of services to both humans and the environment. This article introduces a five-article Wetlands and Coastal Systems Special Collection that evolved from a featured session at the 2015 ASABE Annual International Meeting in New Orleans, Louisiana. The Collection provides perspectives on tools and techniques for enhancing the protection and restoration of wetlands and coastal systems with emphasis on vegetation, hydrology, water quality, and planning. Topics span the Florida Everglades (two articles) and Virginia floodplain (one article) wetland systems and include remote sensing (one article) …

Unmanned Aerial Vehicles For High-Throughput Phenotyping And Agronomic Research, Yeyin Shi, J. Alex Thomasson, Seth C. Murray, N. Ace Pugh, William L. Rooney, Sanaz Shafian, Nithya Rajan, Gregory Rouze, Cristine L. S. Morgan, Haly L. Neely, Aman Rana, Muthu V. Bagavathiannan, James Henrickson, Ezekiel Bowden, John Valasek, Jeff Olsenholler, Michael P. Bishop, Ryan Sheridan, Eric B. Putman, Sorin Popescu, Travis Burks, Dale Cope, Amir Ibrahim, Billy F. Mccutchen, David D. Baltensperger, Robert V. Avant, Jr., Misty Vidrine, Chenghai Yang

Biological Systems Engineering: Papers and Publications

Advances in automation and data science have led agriculturists to seek real-time, high quality, high-volume crop data to accelerate crop improvement through breeding and to optimize agronomic practices. Breeders have recently gained massive data-collection capability in genome sequencing of plants. Faster phenotypic trait data collection and analysis relative to genetic data leads to faster and better selections in crop improvement. Furthermore, faster and higher-resolution crop data collection leads to greater capability for scientists and growers to improve precision-agriculture practices on increasingly larger farms; e.g., site-specific application of water and nutrients. Unmanned aerial vehicles (UAVs) have recently gained traction as agricultural …

Development And Assessment Of A Groundwater Sustainability Index In Climatically Diverse Groundwater Irrigated Regions In Nebraska, Maria A. Mulet Jalil

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

The aim of this research was to evaluate the impact of regional change in ET on groundwater level changes and the assessment and development of a groundwater sustainability index for climatically diverse regions across Nebraska during 2000-2014. Irrigation in the selected regions is predominantly supplied by groundwater. The hypothesis is that groundwater use can become sustainable if the regional evapotranspiration (ET) is managed so that it equals the ET of vegetation that is native to the region. Site locations were Box Butte, Chase, Dundy, Holt LNNRD and York Counties and 3 ecosystems were evaluated: native vegetation, dryland and irrigated cropping …

High-Throughput Screening Of Clinically Approved Drugs That Prime Polyethylenimine Transfection Reveals Modulation Of Mitochondria Dysfunction Response Improves Gene Transfer Efficiencies, Albert Nguyen, Jared P. Beyersdorf, Jean-Jack Riethoven, Angela K. Pannier

Biological Systems Engineering: Papers and Publications

Nonviral gene delivery methods are advantageous over viral vectors in terms of safety, cost, and flexibility in design and application, but suffer from lower gene transfer efficiency. In addition to modifications to nucleic acid design and nonviral carriers, new tools are sought to enhance transfection. Priming is the pharmacological modulation of transfection efficiency and transgene expression, and has demonstrated transfection increase in several compounds, for example, chloroquine and glucocorticoids. To develop a library of transfection priming compounds, a highthroughput screen was performed of the NIH Clinical Collection (NCC) to identify clinical compounds that prime polyethylenimine (PEI) transfection. HEK293T cells were …

The Experiment And Analysis Of Active Mechanisms For Enhancing Heat And Mass Transfer In Sorption Fluids, Ziqi Shen

Durham School of Architectural Engineering and Construction: Dissertations, Thesis, and Student Research

This project was funded by American Society of Heating, Refrigerating, and Air-Conditioning Engineers (ASHRAE RP-1462). It is a three years’ research, including the literature review, labs construction, experiments and data analysis.

In this thesis, first of all, we conducted literature review of mechanism motion influence on heat and mass transfer and additive effect in absorption chiller. This part helps us understand the basic idea of how mechanism motion affects the heat and mass transfer of sorption fluids and gives us reference on how to select the experiment instrument and the experiment operation range.

In the second part, the instrument selection …

Carbon Nanotubes Affect The Toxicity Of Cuo Nanoparticles To Denitrification In Marine Sediments By Altering Cellular Internalization Of Nanoparticle, Xiong Zheng, Yinglong Su, Yinguang Chen, Rui Wan, Mu Li, Haining Huang, Xu Li

Department of Civil and Environmental Engineering: Faculty Publications

Denitrification is an important pathway for nitrate transformation in marine sediments, and this process has been observed to be negatively affected by engineered nanomaterials. However, previous studies only focused on the potential effect of a certain type of nanomaterial on microbial denitrification. Here we show that the toxicity of CuO nanoparticles (NPs) to denitrification in marine sediments is highly affected by the presence of carbon nanotubes (CNTs). It was found that the removal efficiency of total NO_X⁻-N (NO₃⁻-N and NO₂⁻-N) in the presence of CuO NPs was only 62.3%, but it …

Efficiency Of Chlorophyll In Gross Primary Productivity: A Proof Of Concept And Application In Crops, Anatoly A. Gitelson, Yi Peng, Andrés Viña, Timothy J. Arkebauer, James S. Schepers

Biological Systems Engineering: Papers and Publications

One of the main factors affecting vegetation productivity is absorbed light, which is largely governed by chlorophyll. In this paper, we introduce the concept of chlorophyll efficiency, representing the amount of gross primary production per unit of canopy chlorophyll content (Chl) and incident PAR. We analyzed chlorophyll efficiency in two contrasting crops (soybean and maize). Given that they have different photosynthetic pathways (C3 vs. C4), leaf structures (dicot vs. monocot) and canopy architectures (a heliotrophic leaf angle distribution vs. a spherical leaf angle distribution), they cover a large spectrum of biophysical conditions. Our results show that chlorophyll efficiency in primary …

Water And Energy Use Of Antimicrobial Interventions In A Mid-Size Beef Packing Plant, Rami M. M. Ziara, Shaobin Li, Bruce I. Dvorak, Jeyamkondan Subbiah

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Data regarding the water and energy usage of current antimicrobial interventions in beef packing plants is scarce. The objective of this study was to collect representative water and energy usage data in a beef packing plant, with emphasis on antimicrobial interventions, to provide baseline data for comparison of new intervention technologies developed by researchers. Permanent and portable water flow meters were installed on the plant’s plumbing system to collect water flow data from March 2014 to March 2015. A local utility company was hired to meter electricity at the different subsystems using portable data loggers. The natural gas used in …

Microbial Degradation Of Sulfonamide Antibiotics, Rachel Levine

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Certain microbes can transform antibiotics in the environment. However, little is known about the identity of these microbes and their antibiotic biotransformation processes. The objectives of this study were to (1) isolate bacterial strains capable of transforming antibiotics, (2) determine the biotransformation kinetics of antibiotics, (3) characterize the effects of background carbons on the biotransformation kinetics, and (4) identify biotransformation products under various environmental conditions. Sulfadiazine (SDZ) was used as the model antibiotic in this study due to its frequent occurrence in livestock wastes. Surface soil from a cattle feedlot was collected to enrich potential SDZ degrading bacteria. A mixed …

The Influence Of Sediment Characteristics On The Fate Of Steroidogenic Compounds In Aquatic Systems And The Effects On Progesterone Bioavailability In A Target Organism, Jodi L. Sangster

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

There is growing concern about the biologic effects stemming from steroids in impacted waterways. In aquatic systems, interaction between steroids and sediment influence both contaminant fate as well as subsequent bioavailability to aquatic organisms. The focus of this dissertation research was to gain a better understanding of steroid behavior in aquatic systems based on the physiochemical properties of sediment and to use this knowledge to better understand the biological effects stemming from sediment-associated progesterone exposure. Two natural aquatic sediments, a sand and a silty loam, were selected to represent marked differences in sediments properties. Initially, sorption of 17β-estradiol, estrone, progesterone, …

Micro- And Nanoparticulates For Dna Vaccine Delivery, Eric Farris, Deborah M. Brown, Amanda Ramer-Tait, Angela K. Pannier

Biological Systems Engineering: Papers and Publications

DNA vaccination has emerged as a promising alternative to traditional protein-based vaccines for the induction of protective immune responses. DNA vaccines offer several advantages over traditional vaccines, including increased stability, rapid and inexpensive production, and flexibility to produce vaccines for a wide variety of infectious diseases. However, the immunogenicity of DNA vaccines delivered as naked plasmid DNA is often weak due to degradation of the DNA by nucleases and inefficient delivery to immune cells. Therefore, biomaterial-based delivery systems based on micro- and nanoparticles that encapsulate plasmid DNA represent the most promising strategy for DNA vaccine delivery. Microparticulate delivery systems allow …

Ecosystem Evapotranspiration: Challenges In Measurements, Estimates, And Modeling, D.M. Amatya, Suat Irmak, P. Gowda, G. Sun, J.E. Nettles, K.R. Douglas-Mankin

Biological Systems Engineering: Papers and Publications

Evapotranspiration (ET) processes at the leaf to landscape scales in multiple land uses have important controls and feed backs for local, regional, and global climate and water resource systems. Innovative methods, tools, and technologies for improved understanding and quantification of ET and crop water use are critical for adapting more effective management strategies to cope with increasing demand for freshwater resources under global climate change. This article introduces an ASABE Special Collection of 12 articles on ET monitoring and modeling research for multiple land uses and scales. The collection focuses on recent advances in four critical topical areas: (1) reference …

Glucocorticoid Cell Priming Enhances Transfection Outcomes In Adult Human Mesenchymal Stem Cells, Abby M. Kelly, Sarah A. Plautz, Janos Zempleni, Angela K. Pannier

Biological Systems Engineering: Papers and Publications

Human mesenchymal stem cells (hMSCs) are one of the most widely researched stem cell types with broad applications from basic research to therapeutics, the majority of which require introduction of exogenous DNA. However, safety and scalability issues hinder viral delivery, while poor efficiency hinders nonviral gene delivery, particularly to hMSCs. Here, we present the use of a pharmacologic agent (glucocorticoid) to overcome barriers to hMSC DNA transfer to enhance transfection using three common nonviral vectors. Glucocorticoid priming significantly enhances transfection in hMSCs, demonstrated by a 3-fold increase in efficiency, 4–15-fold increase in transgene expression, and prolonged transgene expression when compared …

A Five-Year Performance Review Of Field-Scale, Slow-Release Permanganate Candles With Recommendations For Second-Generation Improvements, Mark Christenson, Ann Kambhu, James Reece, Steve D. Comfort, Laurie Brunner

Nebraska Water Center: Faculty Publications

In 2009, we identified a TCE plume at an abandoned landfill that was located in a low permeable siltyclay aquifer. To treat the TCE, we manufactured slow-release potassium permanganate cylinders (oxidant candles) that had diameters of either 5.1 or 7.6 cm and were 91.4 cm long. In 2010, we compared two methods of candle installation by inserting equal masses of the oxidant candles (7.6-cm vs 5.1-cm dia). The 5.1-cm dia candles were inserted with direct-push rods while the 7.6-cm candles were housed in screens and lowered into 10 permanent wells. Since installation, the 7.6-cm oxidant candles have been refurbished approximately …

Recent Morphodynamic Evolution Of The Largest Uninhibited Island In The Yangtze (Changjiang) Estuary During 1998-2014: Influence Of The Anthropogenic Interference, Wen Wei, Xuefei Mei, Zhijun Dai, Zhenghong Tang

Community and Regional Planning Program: Faculty Scholarly and Creative Activity

Estuarine geomorphology worldwide has greatly changed in the Anthropocene due to intensive human inferences in river basin and within estuary, which has received increasing global concerns. Here, recent morphodynamic evolution of Jiuduan Shoal (JDS), the largest uninhabited island in the Yangtze (Changjiang) Estuary, and associated controlling factors were analyzed based on unique high-resolution seasonal-surveyed bathymetric data during 1998–2014. It can be indicated that JDS presents novel 12 and 48 months fluctuations though significant accretion was detected on high flats above −2 m. Meanwhile, morphodynamic evolution of JDS during 1998–2014 was divided into three stages: significant siltation on land-ward half of …

Early Life History Of Three Pelagic-Spawning Minnows Macrhybopsis Spp. In The Lower Missouri River, T. A. Starks, M. L. Miller, J. M. Long

US Army Corps of Engineers

Life-history characteristics of age-0 sturgeon chub Macrhybopsis gelida, shoal chub Macrhybopsis hyostoma and sicklefin chub Macrhybopsis meeki were compared using several methods. All Macrhybopsis species consumed mostly midge pupae, but M. meeki had the most general diet (Levins’ index, B=0⋅22) compared with M. hyostoma (B=0⋅02) and M. gelida (B=0⋅09). Morisita’s diet overlap index among species pairs ranged from 0⋅62 to 0⋅97 and was highest between M. hyostoma and M. gelida. Daily ages estimated from lapilli otoliths for each species ranged from 15 to 43 days for M. gelida, 19 to 44 for …

Critical Factors Affecting The Integration Of Biomass Gasification And Syngas Fermentation Technology, Karthikeyan D. Ramachandriya, Dimple K. Kundiyana, Ashokkumar M. Sharma, Ajay Kumar, Hasan K. Atiyeh, Raymond L. Huhnke, Mark R. Wilkins

Biological Systems Engineering: Papers and Publications

Gasification-fermentation is a thermochemical-biological platform for the production of fuels and chemicals. Biomass is gasified at high temperatures to make syngas, a gas composed of CO, CO2, H2, N2 and other minor components. Syngas is then fed to anaerobic microorganisms that convert CO, CO2 and H2 to alcohols by fermentation. This platform offers numerous advantages such as flexibility of feedstock and syngas composition and lower operating temperature and pressure compared to other catalytic syngas conversion processes. In comparison to hydrolysis-fermentation, gasification-fermentation has a major advantage of utilizing all organic components of biomass, including lignin, to yield higher fuel production. Furthermore, …

Inter-Row Robot Navigation Using 1d Ranging Sensors, Tyler A. Troyer, Santosh Pitla, Ethan Nutter

Biological Systems Engineering: Papers and Publications

In this paper a fuzzy logic navigation controller for an inter-row agricultural robot is developed and evaluated in laboratory settings. The controller receives input from one-dimensional (1D) ranging sensors on the robotic platform, and operated on ten fuzzy rules for basic row-following behavior. The control system was implemented on basic hardware for proof of concept and operated on a commonly available microcontroller development platform and open source software libraries. The robot platform used for experimentation was a small tracked vehicle with differential steering control. Fuzzy inferencing and defuzzification, step response and cross track error were obtained from the test conducted …

262. Direct Measure Of Cervical Interbody Forces In Vivo: Load Reversal After Plating, Eric H. Ledet, Josh Peterson, Rebecca A. Wachs, Mary Beth M. Grabowsky, Joseph C. Glennon, Darryl J. Dirisio

Biological Systems Engineering: Papers and Publications

BACKGROUND CONTEXT: Biomechanics play an important role in spine fusion, but the in vivo biomechanics of the cervical spine are not well characterized and the in vivo biomechanics after spinal arthrodesis have never been studied. Load sharing facilitates fusion, but overloading of interbody implants can lead to subsidence and failure. In vitro studies have demonstrated that anterior plating significantly alters mechanical loading in the cervical spine. The instantaneous axis of rotation is shifted anteriorly and loading is reversed relative to an uninstrumented spine; the interbody space is compressed during extension and unloaded during flexion. However, this has never been tested …

Tractor Hydraulic Power Data Acquisition System, J. B.W. Roeber, Santosh Pitla, Michael F. Kocher, Joe D. Luck, Roger M. Hoy

Biological Systems Engineering: Papers and Publications

Tractor hydraulic power is used on a wide range of agricultural implements; however, the availability of operational hydraulic data at points other than full engine throttle position is limited. Operators could utilize this hydraulic data to maximize field efficiency and minimize machinery costs when determining suitable machinery for field operations. A field usable hydraulic test apparatus capable of measuring tractor hydraulic pressure and flow rate data was developed. The goal of this study was to determine if a hydraulic flow and pressure measurement device could be installed on the rear of a tractor to provide implement hydraulic power consumption at …

Analyses, Calibration And Validation Of Evapotranspirationmodels To Predict Grass-Reference Evapotranspiration In Thesenegal River Delta, Koffi Djaman, Hossein Tabari, Alpha B. Balde, Lamine Diop, Koichi Futakuchi, Suat Irmak

Biological Systems Engineering: Papers and Publications

Study region: Grass-reference evapotranspiration estimation by the Penman-Monteithmethod (PM-ETo) requires a number of climate variables which are not always availableat all weather stations. Different alternative ETo equations have been developed and theirutilization for various local climate conditions requires analyses of their accuracy as com-pared to the standardized Penman-Monteith method. There is a significant lack of data andinformation on this topic in the Senegal River Delta (SRD). Study focus: The objective of this study was to evaluate, calibrate and validate six EToequations ((Trabert, Mahringer, Penman1948, Albrecht, Valiantzas1 and Valiantzas2) forthe SRD. Although all six equations showed good agreement with the PM-ETo …

Objective Climatological Analysis Of Extreme Weather Events In Arizona During The North American Monsoon, Jeremy J. Mazon, Christopher L. Castro, David K. Adams, Hsin-I Chang, Carlos M. Carrillo, John J. Brost

Biological Systems Engineering: Papers and Publications

Almost one-half of the annual precipitation in the southwestern United States occurs during the North American monsoon (NAM). Given favorable synoptic-scale conditions, organized monsoon thunderstorms may affect relatively large geographic areas. Through an objective analysis of atmospheric reanalysis and observational data, the dominant synoptic patterns associated with NAM extreme events are determined for the period from 1993 to 2010. Thermodynamically favorable extreme-weather-event days are selected on the basis of atmospheric instability and precipitable water vapor from Tucson, Arizona, rawinsonde data. The atmospheric circulation patterns at 500 hPa associated with the extreme events are objectively characterized using principal component analysis. The …

Calibration Procedure For Fuel Flow Meters At The Nebraska Tractor Test Lab, Michael F. Kocher, Matthew T. Wold, Roger M. Hoy, Austin Lammers, Erin E. Blankenship

Biological Systems Engineering: Papers and Publications

Reports in the literature indicated several factors that can influence the accuracy of Coriolis Effect mass flow meters. A Coriolis Effect mass flow meter is used to verify tractor manufacturer’s fuel consumption claims at the Nebraska Tractor Test Laboratory (NTTL). The accuracy requirement placed on the flow meter by the Organization for Economic Co-operation and Development (OECD) in the Code 2 tractor performance test procedure is not clear, but in the most conservative interpretation is ±0.5% of each flow rate measured. Results showed a dynamic weighing calibration method was not accurate enough to obtain a calibration of the flow meter …

Nebraska Water Center Annual Report 2016, Nebraska Water Center

Literature from The Nebraska Water Center

Contents

Foreword

Director’s Letter

Since 1964: The Nebraska Water Center

Building the future

Nebraska’s Top 10 water challenges

Nebraska Water Center advisory board

Water Resources Advisory Panel: A key to success

Nebraska Water Sciences Laboratory

USGS 104b projects for 2016

Ogallala Aquifer focus of USDA research grant

Graduate research on amphetamines draws attention

NIC hosts water symposium and law conference

Water seminar lectures key on “Water and health”

Four picked for IRES program in Czech Republic

WARI program begins

2016 tour to Colorado’s South Platte basin

45th annual tour visits Platte River basin in Colorado

McCornick new Water for Food …

Nebraska Water Center (Brochure ), Nebraska Water Center

Literature from The Nebraska Water Center

The Nebraska Water Center was established in 1964 as one of 54 Water Resources Research Institutes nationwide with passage of the first Clean Water Act. It facilitates the University of Nebraska-Lincoln’s land grant missions in research, extension and teaching in water science, law and policy to address water quantity and quality issues of priority to Nebraska. NWC helps water researchers by awarding small grants, conducting water-related education and outreach activities for stakeholders, and disseminating research results through media, colloquia, conferences, lectures, and tours. NWC provides state-of-the-art analytical technology to conduct water research and education to scientists and engineers, helping them …

Temporal Dynamics Of Maize Plant Growth, Water Use, And Leaf Water Content Using Automated High Throughput Rgb And Hyperspectral Imaging, Yufeng Ge, Geng Bai, Vincent Stoerger, James C. Schnable

Biological Systems Engineering: Papers and Publications

Automated collection of large scale plant phenotype datasets using high throughput imaging systems has the potential to alleviate current bottlenecks in data-driven plant breeding and crop improvement. In this study, we demonstrate the characterization of temporal dynamics of plant growth and water use, and leaf water content of two maize genotypes under two different water treatments. RGB (Red Green Blue) images are processed to estimate projected plant area, which are correlated with destructively measured plant shoot fresh weight (FW), dry weight (DW) and leaf area. Estimated plant FW and DW, along with pot weights, are used to derive daily plant …

Impact Of Scale/Resolution On Evapotranspiration From Landsat And Modis Images, Vivek Sharma, Ayse Kilic, Suat Irmak

Biological Systems Engineering: Papers and Publications

Understanding the role of landscape heterogeneity and its influence on the scaling behavior of surface fluxes as observed by satellite sensors with different spatial resolutions is a critical need to investigate. In this study, the effects of pixel scales on ETc estimation and other parameters that are used to calculate ETc were investigated over different vegetation surfaces in south central Nebraska, USA. Surface Energy Balance System (SEBS) was used to estimate spatially distributed ETc by combining ground-based meteorological data for Landsat and MODIS imagery. The estimated surface energy fluxes were compared and validated to the measured Bowen Ratio Energy Balance …