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Full-Text Articles in Environmental Engineering

Lucidpipe™ Power System, Purdue Ect Team Dec 2016

Lucidpipe™ Power System, Purdue Ect Team

ECT Fact Sheets

In most gravity fed water transmission pipelines, it is desired to reduce excess pressure head to prevent undue strain on a pipeline and lower the incidence of leaks. Normally this is done with pressure reducing valves that essentially burn off this excess pressure as heat. The LucidPipe™ system converts it to low cost electricity thereby removing unwanted pressure and generating energy at the same time - energy that can be put used behind the grid or put back on the grid. The LucidPipe™ system extracts a small percentage of pressure head providing nearly invisible operation allowing water operators to fulfill …


Modeling Streambank Erosion On Composite Streambanks On A Watershed Scale, A. R. Mittelstet, D. E. Storm, G. A. Fox, P. M. Allen Dec 2016

Modeling Streambank Erosion On Composite Streambanks On A Watershed Scale, A. R. Mittelstet, D. E. Storm, G. A. Fox, P. M. Allen

Department of Biological Systems Engineering: Papers and Publications

Streambanks can be a significant source of sediment and phosphorus to aquatic ecosystems. Although the streambank-erosion routine in the Soil and Water Assessment Tool (SWAT) has improved in recent versions, the recently developed routine in SWAT 2012 has undergone limited testing, and the lack of site or watershed specific streambank data increases the uncertainty in the streambank-erosion predictions. There were two primary objectives of this research: (1) modify and test the 2012 SWAT streambank-erosion routine on composite streambanks, and (2) compare SWAT default and field-measured channel parameters and assess their influence on predicted streambank erosion. Three modifications were made to …


Quantifying An Aquifer Nitrate Budget And Future Nitrate Discharge Using Field Data From Streambeds And Well Nests, Troy E. Gilmore, David P. Genereux, Kathleen M. Farrell, Helena Mitasova Nov 2016

Quantifying An Aquifer Nitrate Budget And Future Nitrate Discharge Using Field Data From Streambeds And Well Nests, Troy E. Gilmore, David P. Genereux, Kathleen M. Farrell, Helena Mitasova

Department of Biological Systems Engineering: Papers and Publications

Novel groundwater sampling (age, flux, and nitrate) carried out beneath a streambed and in wells was used to estimate (1) the current rate of change of nitrate storage, dSNO3 /dt, in a contaminated unconfined aquifer, and (2) future [NO3]FWM (the flow-weighted mean nitrate concentration in groundwater discharge) and fNO3 (the nitrate flux from aquifer to stream). Estimates of dSNO3 /dt suggested that at the time of sampling (2013) the nitrate storage in the aquifer was decreasing at an annual rate (mean = –9 mmol/m2yr) equal …


Effect Of Rainfall Timing And Tillage On The Transport Of Steroidhormones In Runoff From Manure Amended Row Crop Fields, Sagor Biswas, William L. Kranz, Charles A. Shapiro, Daniel D. Snow, Shannon L. Bartelt-Hunt, Mitiku Mamo, David D. Tarkalson, Tian C. Zhang, David P. Shelton, Simon J. Van Donk, Terry L. Mader Nov 2016

Effect Of Rainfall Timing And Tillage On The Transport Of Steroidhormones In Runoff From Manure Amended Row Crop Fields, Sagor Biswas, William L. Kranz, Charles A. Shapiro, Daniel D. Snow, Shannon L. Bartelt-Hunt, Mitiku Mamo, David D. Tarkalson, Tian C. Zhang, David P. Shelton, Simon J. Van Donk, Terry L. Mader

Department of Biological Systems Engineering: Papers and Publications

Runoff generated from livestock manure amended row crop fields is one of the major pathways of hormone transport to the aquatic environment. The study determined the effects of manure handling, tillage methods, and rainfall timing on the occurrence and transport of steroid hormones in runoff from the row crop field. Stockpiled and composted manure from hormone treated and untreated animals were applied to test plots and subjected to two rainfall simulation events 30 days apart. During the two rainfall simulation events, detection of any steroid hormone or metabolites was identified in 8–86% of runoff samples from any tillage and manure …


Wetlands And Coastal Systems: Protecting And Restoring Valuable Ecosystems, C. T. Agouridis, K. R. Douglas-Mankin, A. C. Linhoss, A. R. Mittelstet Sep 2016

Wetlands And Coastal Systems: Protecting And Restoring Valuable Ecosystems, C. T. Agouridis, K. R. Douglas-Mankin, A. C. Linhoss, A. R. Mittelstet

Department of Biological Systems Engineering: Papers and Publications

Wetlands and coastal systems are unique, highly productive, and often threatened landscapes that provide a host of services to both humans and the environment. This article introduces a five-article Wetlands and Coastal Systems Special Collection that evolved from a featured session at the 2015 ASABE Annual International Meeting in New Orleans, Louisiana. The Collection provides perspectives on tools and techniques for enhancing the protection and restoration of wetlands and coastal systems with emphasis on vegetation, hydrology, water quality, and planning. Topics span the Florida Everglades (two articles) and Virginia floodplain (one article) wetland systems and include remote sensing (one article) …


University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 10 - Play 3 - 2016/06/15), David Hanson Aug 2016

University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 10 - Play 3 - 2016/06/15), David Hanson

Energize New Mexico (NSF EPSCoR Track 1 IV)

Collect the photosynthetic rate of poplar plants under different isotopic compositions of CO2 with the TDL machine and with the Licor equipment.


University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 1/2 - Play 3 - 2016/06/12), David Hanson Aug 2016

University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 1/2 - Play 3 - 2016/06/12), David Hanson

Energize New Mexico (NSF EPSCoR Track 1 IV)

Collect the photosynthetic rate of poplar plants under different isotopic compositions of CO2 with the TDL machine and with the Licor equipment.


University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 1 - Play 2 - 2016/06/07), David Hanson Aug 2016

University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 1 - Play 2 - 2016/06/07), David Hanson

Energize New Mexico (NSF EPSCoR Track 1 IV)

Collect the photosynthetic rate of poplar plants under different isotopic compositions of CO2 with the TDL machine and with the Licor equipment.


University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 1/3 - Play 1 - 2016/06/08), David Hanson Aug 2016

University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 1/3 - Play 1 - 2016/06/08), David Hanson

Energize New Mexico (NSF EPSCoR Track 1 IV)

Collect the photosynthetic rate of poplar plants under different isotopic compositions of CO2 with the TDL machine and with the Licor equipment.


University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 10 - Play 2 - 2016/06/14), David Hanson Aug 2016

University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 10 - Play 2 - 2016/06/14), David Hanson

Energize New Mexico (NSF EPSCoR Track 1 IV)

Collect the photosynthetic rate of poplar plants under different isotopic compositions of CO2 with the TDL machine and with the Licor equipment.


University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 1/3 - Play 2 - 2016/06/08), David Hanson Aug 2016

University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 1/3 - Play 2 - 2016/06/08), David Hanson

Energize New Mexico (NSF EPSCoR Track 1 IV)

Collect the photosynthetic rate of poplar plants under different isotopic compositions of CO2 with the TDL machine and with the Licor equipment.


University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 10 - Play 1 - 2016/06/10), David Hanson Aug 2016

University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 10 - Play 1 - 2016/06/10), David Hanson

Energize New Mexico (NSF EPSCoR Track 1 IV)

Collect the photosynthetic rate of poplar plants under different isotopic compositions of CO2 with the TDL machine and with the Licor equipment.


University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 1/2 - Play 3 - 2016/06/12), David Hanson Aug 2016

University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 1/2 - Play 3 - 2016/06/12), David Hanson

Energize New Mexico (NSF EPSCoR Track 1 IV)

Collect the photosynthetic rate of poplar plants under different isotopic compositions of CO2 with the TDL machine and with the Licor equipment.


University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 12 - Play 2 - 2016/06/15), David Hanson Aug 2016

University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 12 - Play 2 - 2016/06/15), David Hanson

Energize New Mexico (NSF EPSCoR Track 1 IV)

Collect the photosynthetic rate of poplar plants under different isotopic compositions of CO2 with the TDL machine and with the Licor equipment. To observe the difference in the assimilation of both isotopes of Carbon Dioxide.


University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 12 - Play 3 - 2016/06/16), David Hanson Aug 2016

University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 12 - Play 3 - 2016/06/16), David Hanson

Energize New Mexico (NSF EPSCoR Track 1 IV)

Collect the photosynthetic rate of poplar plants under different isotopic compositions of CO2 with the TDL machine and with the Licor equipment. To observe the difference in the assimilation of both isotopes of Carbon Dioxide.


University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 11 - Play 2 - 2016/06/15), David Hanson Aug 2016

University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 11 - Play 2 - 2016/06/15), David Hanson

Energize New Mexico (NSF EPSCoR Track 1 IV)

Collect the photosynthetic rate of poplar plants under different isotopic compositions of CO2 with the TDL machine and with the Licor equipment. To observe the difference in the assimilation of both isotopes of Carbon Dioxide.


University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 16 - Play 1 - 2016/06/20), David Hanson Aug 2016

University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 16 - Play 1 - 2016/06/20), David Hanson

Energize New Mexico (NSF EPSCoR Track 1 IV)

Collect the photosynthetic rate of poplar plants under different isotopic compositions of CO2 with the TDL machine and with the Licor equipment. To observe the difference in the assimilation of both isotopes of Carbon Dioxide.


University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 11 - Play 1 - 2016/06/15), David Hanson Aug 2016

University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 11 - Play 1 - 2016/06/15), David Hanson

Energize New Mexico (NSF EPSCoR Track 1 IV)

Collect the photosynthetic rate of poplar plants under different isotopic compositions of CO2 with the TDL machine and with the Licor equipment. To observe the difference in the assimilation of both isotopes of Carbon Dioxide.


University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 10/11 - Play 4 - 2016/06/21), David Hanson Aug 2016

University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 10/11 - Play 4 - 2016/06/21), David Hanson

Energize New Mexico (NSF EPSCoR Track 1 IV)

Collect the photosynthetic rate of poplar plants under different isotopic compositions of CO2 with the TDL machine and with the Licor equipment.


University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 10/11 - Play 4 - 2016/06/21), David Hanson Aug 2016

University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 10/11 - Play 4 - 2016/06/21), David Hanson

Energize New Mexico (NSF EPSCoR Track 1 IV)

Collect the photosynthetic rate of poplar plants under different isotopic compositions of CO2 with the TDL machine and with the Licor equipment.


University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 12 - Play 1 - 2016/06/15), David Hanson Aug 2016

University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 12 - Play 1 - 2016/06/15), David Hanson

Energize New Mexico (NSF EPSCoR Track 1 IV)

Collect the photosynthetic rate of poplar plants under different isotopic compositions of CO2 with the TDL machine and with the Licor equipment. To observe the difference in the assimilation of both isotopes of Carbon Dioxide.


University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 16 - Play 2 - 2016/06/20), David Hanson Aug 2016

University Of New Mexico Bioalgal Energy, Photosynthesis Rates Of Poplar Plants - Measurements With Tdl Machine (Enriched Co2 - Enriched-Exposed - Tray 16 - Play 2 - 2016/06/20), David Hanson

Energize New Mexico (NSF EPSCoR Track 1 IV)

Collect the photosynthetic rate of poplar plants under different isotopic compositions of CO2 with the TDL machine and with the Licor equipment. To observe the difference in the assimilation of both isotopes of Carbon Dioxide.


Miss Lonesome: Old Boats Past Their Prime, Garth Woodruff Aug 2016

Miss Lonesome: Old Boats Past Their Prime, Garth Woodruff

Faculty Publications

No abstract provided.


Unmanned Aerial Vehicles For High-Throughput Phenotyping And Agronomic Research, Yeyin Shi, J. Alex Thomasson, Seth C. Murray, N. Ace Pugh, William L. Rooney, Sanaz Shafian, Nithya Rajan, Gregory Rouze, Cristine L. S. Morgan, Haly L. Neely, Aman Rana, Muthu V. Bagavathiannan, James Henrickson, Ezekiel Bowden, John Valasek, Jeff Olsenholler, Michael P. Bishop, Ryan Sheridan, Eric B. Putman, Sorin Popescu, Travis Burks, Dale Cope, Amir Ibrahim, Billy F. Mccutchen, David D. Baltensperger, Robert V. Avant, Jr., Misty Vidrine, Chenghai Yang Jul 2016

Unmanned Aerial Vehicles For High-Throughput Phenotyping And Agronomic Research, Yeyin Shi, J. Alex Thomasson, Seth C. Murray, N. Ace Pugh, William L. Rooney, Sanaz Shafian, Nithya Rajan, Gregory Rouze, Cristine L. S. Morgan, Haly L. Neely, Aman Rana, Muthu V. Bagavathiannan, James Henrickson, Ezekiel Bowden, John Valasek, Jeff Olsenholler, Michael P. Bishop, Ryan Sheridan, Eric B. Putman, Sorin Popescu, Travis Burks, Dale Cope, Amir Ibrahim, Billy F. Mccutchen, David D. Baltensperger, Robert V. Avant, Jr., Misty Vidrine, Chenghai Yang

Department of Biological Systems Engineering: Papers and Publications

Advances in automation and data science have led agriculturists to seek real-time, high quality, high-volume crop data to accelerate crop improvement through breeding and to optimize agronomic practices. Breeders have recently gained massive data-collection capability in genome sequencing of plants. Faster phenotypic trait data collection and analysis relative to genetic data leads to faster and better selections in crop improvement. Furthermore, faster and higher-resolution crop data collection leads to greater capability for scientists and growers to improve precision-agriculture practices on increasingly larger farms; e.g., site-specific application of water and nutrients. Unmanned aerial vehicles (UAVs) have recently gained traction as agricultural …


High-Throughput Screening Of Clinically Approved Drugs That Prime Polyethylenimine Transfection Reveals Modulation Of Mitochondria Dysfunction Response Improves Gene Transfer Efficiencies, Albert Nguyen, Jared P. Beyersdorf, Jean-Jack Riethoven, Angela K. Pannier Jul 2016

High-Throughput Screening Of Clinically Approved Drugs That Prime Polyethylenimine Transfection Reveals Modulation Of Mitochondria Dysfunction Response Improves Gene Transfer Efficiencies, Albert Nguyen, Jared P. Beyersdorf, Jean-Jack Riethoven, Angela K. Pannier

Department of Biological Systems Engineering: Papers and Publications

Nonviral gene delivery methods are advantageous over viral vectors in terms of safety, cost, and flexibility in design and application, but suffer from lower gene transfer efficiency. In addition to modifications to nucleic acid design and nonviral carriers, new tools are sought to enhance transfection. Priming is the pharmacological modulation of transfection efficiency and transgene expression, and has demonstrated transfection increase in several compounds, for example, chloroquine and glucocorticoids. To develop a library of transfection priming compounds, a highthroughput screen was performed of the NIH Clinical Collection (NCC) to identify clinical compounds that prime polyethylenimine (PEI) transfection. HEK293T cells were …


Efficiency Of Chlorophyll In Gross Primary Productivity: A Proof Of Concept And Application In Crops, Anatoly A. Gitelson, Yi Peng, Andrés Viña, Timothy J. Arkebauer, James S. Schepers Jun 2016

Efficiency Of Chlorophyll In Gross Primary Productivity: A Proof Of Concept And Application In Crops, Anatoly A. Gitelson, Yi Peng, Andrés Viña, Timothy J. Arkebauer, James S. Schepers

Department of Biological Systems Engineering: Papers and Publications

One of the main factors affecting vegetation productivity is absorbed light, which is largely governed by chlorophyll. In this paper, we introduce the concept of chlorophyll efficiency, representing the amount of gross primary production per unit of canopy chlorophyll content (Chl) and incident PAR. We analyzed chlorophyll efficiency in two contrasting crops (soybean and maize). Given that they have different photosynthetic pathways (C3 vs. C4), leaf structures (dicot vs. monocot) and canopy architectures (a heliotrophic leaf angle distribution vs. a spherical leaf angle distribution), they cover a large spectrum of biophysical conditions. Our results show that chlorophyll efficiency in primary …


Micro- And Nanoparticulates For Dna Vaccine Delivery, Eric Farris, Deborah M. Brown, Amanda Ramer-Tait, Angela K. Pannier Apr 2016

Micro- And Nanoparticulates For Dna Vaccine Delivery, Eric Farris, Deborah M. Brown, Amanda Ramer-Tait, Angela K. Pannier

Department of Biological Systems Engineering: Papers and Publications

DNA vaccination has emerged as a promising alternative to traditional protein-based vaccines for the induction of protective immune responses. DNA vaccines offer several advantages over traditional vaccines, including increased stability, rapid and inexpensive production, and flexibility to produce vaccines for a wide variety of infectious diseases. However, the immunogenicity of DNA vaccines delivered as naked plasmid DNA is often weak due to degradation of the DNA by nucleases and inefficient delivery to immune cells. Therefore, biomaterial-based delivery systems based on micro- and nanoparticles that encapsulate plasmid DNA represent the most promising strategy for DNA vaccine delivery. Microparticulate delivery systems allow …


A Five-Year Performance Review Of Field-Scale, Slow-Release Permanganate Candles With Recommendations For Second-Generation Improvements, Mark Christenson, Ann Kambhu, James Reece, Steve D. Comfort, Laurie Brunner Feb 2016

A Five-Year Performance Review Of Field-Scale, Slow-Release Permanganate Candles With Recommendations For Second-Generation Improvements, Mark Christenson, Ann Kambhu, James Reece, Steve D. Comfort, Laurie Brunner

Nebraska Water Center: Faculty Publications

In 2009, we identified a TCE plume at an abandoned landfill that was located in a low permeable siltyclay aquifer. To treat the TCE, we manufactured slow-release potassium permanganate cylinders (oxidant candles) that had diameters of either 5.1 or 7.6 cm and were 91.4 cm long. In 2010, we compared two methods of candle installation by inserting equal masses of the oxidant candles (7.6-cm vs 5.1-cm dia). The 5.1-cm dia candles were inserted with direct-push rods while the 7.6-cm candles were housed in screens and lowered into 10 permanent wells. Since installation, the 7.6-cm oxidant candles have been refurbished approximately …


Ecosystem Evapotranspiration: Challenges In Measurements, Estimates, And Modeling, D.M. Amatya, Suat Irmak, P. Gowda, G. Sun, J.E. Nettles, K.R. Douglas-Mankin Feb 2016

Ecosystem Evapotranspiration: Challenges In Measurements, Estimates, And Modeling, D.M. Amatya, Suat Irmak, P. Gowda, G. Sun, J.E. Nettles, K.R. Douglas-Mankin

Department of Biological Systems Engineering: Papers and Publications

Evapotranspiration (ET) processes at the leaf to landscape scales in multiple land uses have important controls and feed backs for local, regional, and global climate and water resource systems. Innovative methods, tools, and technologies for improved understanding and quantification of ET and crop water use are critical for adapting more effective management strategies to cope with increasing demand for freshwater resources under global climate change. This article introduces an ASABE Special Collection of 12 articles on ET monitoring and modeling research for multiple land uses and scales. The collection focuses on recent advances in four critical topical areas: (1) reference …


Glucocorticoid Cell Priming Enhances Transfection Outcomes In Adult Human Mesenchymal Stem Cells, Abby M. Kelly, Sarah A. Plautz, Janos Zempleni, Angela K. Pannier Feb 2016

Glucocorticoid Cell Priming Enhances Transfection Outcomes In Adult Human Mesenchymal Stem Cells, Abby M. Kelly, Sarah A. Plautz, Janos Zempleni, Angela K. Pannier

Department of Biological Systems Engineering: Papers and Publications

Human mesenchymal stem cells (hMSCs) are one of the most widely researched stem cell types with broad applications from basic research to therapeutics, the majority of which require introduction of exogenous DNA. However, safety and scalability issues hinder viral delivery, while poor efficiency hinders nonviral gene delivery, particularly to hMSCs. Here, we present the use of a pharmacologic agent (glucocorticoid) to overcome barriers to hMSC DNA transfer to enhance transfection using three common nonviral vectors. Glucocorticoid priming significantly enhances transfection in hMSCs, demonstrated by a 3-fold increase in efficiency, 4–15-fold increase in transgene expression, and prolonged transgene expression when compared …