Environmental Engineering Commons^™

Microbial Degradation Of Sulfonamide Antibiotics, Rachel Levine

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Certain microbes can transform antibiotics in the environment. However, little is known about the identity of these microbes and their antibiotic biotransformation processes. The objectives of this study were to (1) isolate bacterial strains capable of transforming antibiotics, (2) determine the biotransformation kinetics of antibiotics, (3) characterize the effects of background carbons on the biotransformation kinetics, and (4) identify biotransformation products under various environmental conditions. Sulfadiazine (SDZ) was used as the model antibiotic in this study due to its frequent occurrence in livestock wastes. Surface soil from a cattle feedlot was collected to enrich potential SDZ degrading bacteria. A mixed …