Biological Engineering Commons^™

A Modified Surface Energy Balance For Modeling Evapotranspiration And Canopy Resistance, Luis O. Lagos

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

A modified surface energy balance (SEB) model based on the Shuttleworth-Wallace and Choudhury-Monteith methods was developed to estimate evaporation from soil covered by crop residue, and transpiration from crop canopies. The model describes the energy balance and flux resistances for partially-vegetated and residue-covered surfaces. Physical and biochemical energy storage terms and lateral fluxes are neglected in the model. Net radiation is one of the inputs in the SEB model and provides the energy needed for soil evaporation, crop transpiration and heat transfer through the canopy, soil/residue surfaces and the atmosphere.

A sensitivity analysis of the SEB model parameters showed that …

Department Of Biological Systems Engineering Newsletter, Vol. 4 No. 2, December 2008

BSE Department Magazine

Environmental Management Research
From the Department Head
Meet the Faculty
Alumni News
Comings and Goings
Alumna Profile
In Memoriam
Museum Hand-picked for HELP
Urban Water Quality
Summer Graduates
Distinguished Fellowship Awards
Partners in Pollution Prevention Interns
Quarter-scale Tractor Competition
Scholarships and Ice Cream
BSE faculty develop Odor Footprint Tool By Crystal Powers
Ethanol Co-product Storage and Utilization Conference By John Hay
Biological Systems Engineering Department Hall of Fame: Don Eret and Ned Meier
International Symposium
Faculty & Staff News
University Service Awards
ASABE Annual Meeting

Monitoring Tissue Engineering Using Magnetic Resonance Imaging, Huihui Xu, Shadi F. Othman, Richard L. Magin

Biological Systems Engineering: Papers and Publications

Assessment of tissue regeneration is essential to optimize the stages of tissue engineering (cell proliferation, tissue development and implantation). Optical and X-ray imaging have been used in tissue engineering to provide useful information, but each has limitations: for example, poor depth penetration and radiation damage. Magnetic resonance imaging (MRI) largely overcomes these restrictions, exhibits high resolution (approximately 100 μm) and can be applied both in vitro and in vivo. Recently, MRI has been used in tissue engineering to generate spatial maps of tissue relaxation times (T₁, T₂), water diffusion coefficients, and the stiffness (shear moduli) of …

Extracellular Matrix Protein Orientation By Adsorption On Self-Assembled Monolayers Controls Nonviral Gene Delivery, Beth A. Duensing, Angela K. Pannier

Department of Biological Systems Engineering: Conference Presentations and White Papers

To date, most efforts to understand and improve the efficiency of nonviral gene delivery have focused on altering the physicochemical properties of delivery systems and developing new delivery strategies. The importance of the cellular microenvironment in achieving successful nonviral gene transfer has not been thoroughly examined, though proteins of the extracellular matrix (ECM) mediate cell adhesion and serve to regulate cellular behavior that may, in turn, dictate the cellular responsiveness to nonviral gene delivery. Self-assembled monolayers (SAMs) of alkanethiols on gold were used to control adsorption of ECM molecules and thus control the architecture of the extracellular environment presented to …

Metabolic Engineering Of A Thermophilic Bacterium To Produce Ethanol At High Yield, A. Joe Shaw, Kara K. Podkaminer, Sunil G. Desai, John S. Bardsley, Stephen R. Rogers, Philip G. Thorne, David A. Hogsett, Lee R. Lynd

Dartmouth Scholarship

We report engineering Thermoanaerobacterium saccharolyticum, a thermophilic anaerobic bacterium that ferments xylan and biomass-derived sugars, to produce ethanol at high yield. Knockout of genes involved in organic acid formation (acetate kinase, phosphate acetyltransferase, and L-lactate dehydrogenase) resulted in a strain able to produce ethanol as the only detectable organic product and substantial changes in electron flow relative to the wild type. Ethanol formation in the engineered strain (ALK2) utilizes pyruvate:ferredoxin oxidoreductase with electrons transferred from ferredoxin to NAD(P), a pathway different from that in previously described microbes with a homoethanol fermentation. The homoethanologenic phenotype was stable for >150 generations …

Department Of Biological Systems Engineering Newsletter, Vol. 4 No. 1, August 2008

BSE Department Magazine

Contents:
New Biomedical Lab Makes Debut
From the Department Head
The Dean's List
Meet the Faculty
Alumni News
Student News
UCARE
Graduates
John Miller: A Success Story
Discover Your Career Mechanized Systems Management
Alumnus Profile
Faculty and Staff News
Comings and Goings
Spreading the word about No-till

A Design Aid For Determining Width Of Filter Strips, Michael G. Dosskey, M. J. Helmers, Dean E. Eisenhauer

Biological Systems Engineering: Papers and Publications

Watershed planners need a tool for determining width of filter strips that is accurate enough for developing cost-effective site designs and easy enough to use for making quick determinations on a large number and variety of sites. This study employed the process-based Vegetative Filter Strip Model to evaluate the relationship between filter strip width and trap¬ping efficiency for sediment and water and to produce a design aid for use where specific water quality targets must be met. Model simulations illustrate that relatively narrow filter strips can have high impact in some situations, while in others even a modest impact cannot …

Preparing, Characterizing, On-Line Digital Image Processing Of Residence Time Distribution And Modeling Of Mechanical Properties Of Nanocomposite Foams, Siew Yoong Lee

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

The objectives of this research were to prepare, characterize and to study the effects of organoclay and extrusion variables on the physical, mechanical, structural, thermal and functional properties of tapioca starch (TS)/poly(lactic acid) (PLA) nanocomposite foams. On-line digital imaging processing was used to determine residence time distribution (RTD). Adaptive neuro-fuzzy inference system (ANFIS) was used to model the mechanical properties of nanocomposite foams.

Four different organoclays (Cloisite 10A, 25A, 93A, 15A) were used to produce nanocomposite foams by melt-intercalation. The properties were characterized using Xray diffraction, scanning electron microscopy, differential scanning calorimetric, and Instron universal testing machine. The properties were …

Effects Of Narrow Grass Hedges On Nutrient Transport From Land Application Areas, John E. Gilley, Bahman Eghball, David B. Marx

Department of Biological Systems Engineering: Conference Presentations and White Papers

The placement of stiff-stemmed grass hedges on the contour along a hill slope has been shown to decrease runoff nutrient transport. This study was conducted to measure the effectiveness of a narrow grass hedge in reducing runoff nutrient transport from plots with a range of soil nutrient values. Composted beef cattle manure was applied at dry weights of 0, 68, 105, 142, and 178 Mg ha^-1 to a silty clay loam soil and then incorporated by disking. Soil samples were collected 243 days later for analysis of water-soluble phosphorus (WSP), Bray and Kurtz No.1 phosphorus (Bray-1 P), NO_{3 …}

Nutrient And Microbial Transport From Feedlot Surfaces, John E. Gilley, Elaine D. Berry, Roger A. Eigenberg, David B. Marx, Bryan L. Woodbury

Department of Biological Systems Engineering: Conference Presentations and White Papers

Nutrient and microbial transport by runoff may vary at different locations within a beef cattle feedlot. If the areas making the largest contributions to nutrient and microbial transport can be identified, it may be possible to institute site-specific management practices to reduce runoff nutrient and microbial transport. The objectives of this study were to: a) measure selected feedlot soil properties, and nutrient and microbial transport in runoff from various feedlot locations b) compare the effects of unconsolidated surface materials (USM) (loose manure pack) and consolidated subsurface materials (CSM) (compacted manure and underlying layers) on nutrient and microbial transport, and c) …

Methods To Prioritize Placement Of Riparian Buffers For Improved Water Quality, M. D. Tomer, Michael G. Dosskey, Michael R. Burkart, Matthew J. Helmers, Dean E. Eisenhauer

Biological Systems Engineering: Papers and Publications

Agroforestry buffers in riparian zones can improve stream water quality, provided they intercept and remove contaminants from surface runoff and/or shallow groundwater. Soils, topography, surficial geology, and hydrology determine the capability of forest buffers to intercept and treat these flows. This paper describes two landscape analysis techniques for identifying and mapping locations where agroforestry buffers can effectively improve water quality. One technique employs soil survey information to rank soil map units for how effectively a buffer, when sited on them, would trap sediment from adjacent cropped fields. Results allow soil map units to be compared for relative effectiveness of buffers …

Cellular Arrays (Us Patent Application), Angela K. Pannier, Eric A. Ariazi, V. Craig Jordan, Lonnie D. Shea

Biological Systems Engineering: Papers and Publications

The present invention relates to characterizing transcription within cells. In particular, the present invention provides transfected cell arrays (e.g., two-dimensional and/or three-dimensional arrays) and systems, kits and methods utilizing the same (e.g., for transcriptional activity characterization). Compositions and methods of the present invention find use in, among other things, research, drug discovery and clinical (e.g., diagnostic, preventative and therapeutic) applications.

Narrow Grass Hedge Effects On Nutrient Transport Following Compost Application, John E. Gilley, Bahman Eghball, David B. Marx

Biological Systems Engineering: Papers and Publications

The placement of stiff‐stemmed grass hedges on the contour along a hillslope has been shown to decrease nutrient transport in runoff. This study was conducted to measure the effectiveness of a narrow grass hedge in reducing runoff nutrient transport from plots with a range of soil nutrient values. Composted beef cattle manure was applied at dry weights of 0, 68, 105, 142, and 178 Mg ha^-1 to a silty clay loam soil and then incorporated by disking. Soil samples were collected 243 days later for analysis of water‐soluble phosphorus (WSP), Bray and Kurtz No. 1 phosphorus (Bray‐1 P), NO …

Artificial Neural Network Estimation Of Soil Erosion And Nutrient Concentrations In Runoff From Land Application Areas, Minyoung Kim, John E. Gilley

Biological Systems Engineering: Papers and Publications

The transport of sediment and nutrients from land application areas is an environmental concern. New methods are needed for estimating soil and nutrient concentrations of runoff from cropland areas on which manure is applied. Artificial Neural Networks (ANNs) trained with a backpropagation (BP) algorithm were used to estimate soil erosion, dissolved P (DP) and NH₄–N concentrations of runoff from a land application site near Lincoln, Nebraska, USA. Simulation results from ANN-derived models showed that the amount of soil eroded is positively correlated with rainfall and runoff. In addition, concentrations of DP and NH₄–N in overland flow …

Developing Lcfs For Biofuels: Getting It Right For Corn Ethanol, Kenneth Cassman, Adam Liska

Adam Liska Papers

• Corn ethanol will be first to test the newly developed LCFS assessment methods; substantial amounts of other biofuels will come several years later

• Accurate valuation of direct-effect GHG emissions from corn ethanol is the foundation of the LCFS process; these affects vary with ethanol biorefinery type and corn feedstock supply

• Different reference GHG emissions values are needed for each major class of ethanol plants

• The BESS model provides the most up-to-date, scientifically sound estimate of corn-ethanol GHG emissions; can BESS and GREET reach agreement?

• Certification and compliance tools are also needed

An Adaptive Neuro-Fuzzy Inference System For Modeling Mechanical Properties Of Tapioca Starch-Poly(Lactic Acid) Nanocomposite Foams, Siew-Yoong Lee, Milford Hanna, David D. Jones

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Tapioca starch, poly(lactic acid) and Cloisite 30B nanocomposites foams, with clay contents of 1, 3, 5 and 7% (w/w), were prepared by a meltintercalation method. Multiple inputs single output models were developed to predict radial expansion ratio, unit density, bulk compressibility and spring index of the nanocomposite foams. An individual ANFIS model was developed for each mechanical property using clay content, temperature, pressure and torque as input parameters. All models preformed well with R² values > 0.71. All models had very low root mean squared error values.

Spatial Variations In Nutrient And Microbial Transport From Feedlot Surfaces, John E. Gilley, Elaine D. Berry, Roger A. Eigenberg, David B. Marx, Bryan L. Woodbury

Biological Systems Engineering: Papers and Publications

Nutrient and microbial transport by runoff may vary at different locations within a beef cattle feedlot. If the areas making the largest contributions to nutrient and microbial transport can be identified, it may be possible to institute site‐specific management practices to reduce runoff nutrient and microbial transport. The objectives of this study were to: (1) measure selected feedlot soil properties and nutrient and microbial transport in runoff from various feedlot locations, (2) compare the effects of unconsolidated surface materials (USM) (loose manure pack) and consolidated subsurface materials (CSM) (compacted manure and underlying layers) on nutrient and microbial transport, and (3) …

Preparation And Characterization Of Tapioca Starch–Poly(Lactic Acid) Nanocomposite Foams By Melt Intercalation Based On Clay Type, Siew-Yoong Lee, Han Chen, Milford Hanna

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Abstract Tapioca starch (TS), poly(lactic acid) (PLA), and four different organoclays (Cloisite 10A, Cloisite 25A, Cloisite 93A and Cloisite 15A) were used to produce nanocomposite foams by melt-intercalation. Structural, thermal, physical and mechanical properties were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), differential scanning calorimetry (DSC), and an Instron universal testing machine, respectively. The first XRD peaks for all four nanocomposite foams, were observed to shift to lower angles, indicating that intercalation occurred. The extent of intercalation depended on the type of organoclay and was exhibited in the sequence of Cloisite 10A > 25A > 93A > 15A. Glass transition temperatures …

Pyrene Fate Affected By Humic Acid Amendment In Soil Slurry Systems, Y. Liang, D. Sorensen, Ronald C. Sims, Joan Mclean

Biological Engineering Faculty Publications

Background

Humic acid (HA) has been found to affect the solubility, mineralization, and bound residue formation of polycyclic aromatic hydrocarbons (PAHs). However, most of the studies on the interaction between HA and PAH concentrated on one or two of the three phases. Few studies have provided a simple protocol to demonstrate the overall effects of HA on PAH distribution in soil systems for all three phases.

Methods

In this study, three doses of standard Elliott soil HA (ESHA), 15, 187.5, and 1,875 μg ESHA/g soil slurry, were amended to soil slurry systems. ¹⁴C-pyrene was added to the systems along …

Characterization Of Radio Frequency Heating Of Fresh Fruits Influenced By Dielectric Properties, Sohan Birla, S. Wang, Juming Tang, G. Tiwari

Biological Systems Engineering: Papers and Publications

Because of its fast and volumetric nature, radio frequency (RF) heating has been looked upon as a way to overcome the problems associated with conventional heating methods used for disinfestation of fruits. But non-uniform heating within fruits is a major obstacle in adaptation of this technology. In this study, RF heating patterns influenced by dielectric properties (DPs) of fruits were investigated both experimentally and mathematically. A computer simulation model was developed using FEMLAB 3.4, a commercial software for solving Maxwell’s electromagnetic and Fourier’s heat transfer equations. Orange, apple, grapefruit, peach, and avocado fruits, selected for these studies were subjected to …

Preparation And Characterization Of Tapioca Starch-Poly(Lactic Acid)-Cloisite Na+ Nanocomposite Foams, Siew-Yoong Lee, Milford Hanna

Biological Systems Engineering: Papers and Publications

Tapioca starch, poly(lactic acid), and Cloisite NA⁺ nanocomposite foams, with four clay contents (1, 3, 5, 7, wt %), were prepared by melt-intercalation method. Selected structural, thermal, physical, and mechanical properties were characterized using X-ray diffraction (XRD), scanning electron microscopy, differential scanning calorimetry, and an Instron universal testing machine, respectively. XRD results indicated that the 1 wt % nanocomposite foam did not show the characteristic basal reflection of the nanoclay. The 3, 5, and 7 wt % nanocomposite foams produced a mixture of intercalated and tactoid structures. The d₀₀₁-spacing of 3, 5, 7 wt % nanocomposite foams …

Nutrient Transport As Affected By Rate Of Overland Flow, John E. Gilley, W. F. Sabatka, B. Eghball, David B. Marx

Biological Systems Engineering: Papers and Publications

Little information is currently available concerning the effects of varying flow rate on nutrient transport by overland flow. The objective of this study was to measure the effects of overland flow rate on nutrient transport following the application of beef cattle or swine manure to plots containing 0, 2, 4, or 8 Mg ha^-1 of corn residue. After addition of residue materials to 0.75 m wide by 2.0 m long plots, beef cattle or swine manure was added and the plots were then either disked or maintained in a no-till condition. Three 30 min simulated rainfall events, separated by …

Rule-Based Mamdani-Type Fuzzy Modeling Of Skin Permeability, Deepak R. Keshwani, David D. Jones, George E. Meyer, Rhonda M. Brand

Biological Systems Engineering: Papers and Publications

Two Mamdani type fuzzy models (three inputs–one output and two inputs–one output) were developed to predict the permeability of compounds through human skin. The models were derived from multiple data sources including laboratory data, published data bases, published statistical models, and expert opinion. The inputs to the model include information about the compound (molecular weight and octonal–H₂O partition coefficient) and the application temperature. One model included all three parameters as inputs and the other model only included information about the compound. The values for mole molecular weight ranged from 30 to 600 Da. The values for the log …

Comparison Of Lipid Extraction Methods Of Food-Grade Sorghum (Sorghum Bicolor) Using Hexane, K. L. Christiansen, Curtis L. Weller, V. L. Schlegel, I. M. Dweikat

Biological Systems Engineering: Papers and Publications

Much has been reported concerning sorghum phytochemicals, such as policosanols, but little literature exists on how various extraction methods might be used to optimize the recovery of such phytochemicals. The purpose of this study was to compare three extraction methods for recovery of different sorghum lipids. Lipids were extracted from whole kernel and ground Macia, a food-grade sorghum hybrid, with hexane by a Soxtec method, a refluxing method (RB), and a bench-scale recirculated solvent (RC) method. Comparisons of total lipid yield and lipid class yields extracted from the grain by each method were made. Total lipid yield for the three …

Field Slope Effects On Uniformity Of Corn Seed Spacing For Three Precision Planter Metering Systems, Corey L. Searle, Michael F. Kocher, John A. Smith, Erin E. Blankenship

Biological Systems Engineering: Papers and Publications

The effects of field slope on planter seed spacing uniformity were evaluated for three different seed metering units (cell plate, finger pick‐up, and flat plate) operating with medium round corn seed in a laboratory using the University of Nebraska planter test stand with an opto‐electronic seed spacing sensor system. The metering units included a John Deere MaxEmerge™ Plus VacuMeter row unit with the standard cell corn plate, a John Deere MaxEmerge™ Plus VacuMeter row unit with the flat plate, and a John Deere MaxEmerge™ Plus row unit with the finger pick‐up metering system. Planter seed spacing uniformity was measured using …

Surface Polyethylene Glycol Enhances Substrate-Mediated Gene Delivery By Nonspecifically Immobilized Complexes, Angela K. Pannier, Julie A. Wieland, Lonnie D Shea

Biological Systems Engineering: Papers and Publications

Substrate-mediated gene delivery describes the immobilization of gene therapy vectors to a biomaterial, which enhances gene transfer by exposing adhered cells to elevated DNA concentrations within the local microenvironment. Surface chemistry has been shown to affect transfection by nonspecifically immobilized complexes using self-assembled monolayers (SAMs) of alkanethiols on gold. In this report, SAMs were again used to provide a controlled surface to investigate whether the presence of oligo(ethylene glycol) (EG) groups in a SAM could affect complex morphology and enhance transfection. EG groups were included at percentages that did not affect cell adhesion. Nonspecific complex immobilization to SAMs containing combinations …

Visible/Near-Infrared Hyperspectral Imaging For Beef Tenderness Prediction, Govindarajan Konda Naganathan, Lauren M. Grimes, Jeyamkondan Subbiah, Chris R. Calkins, Ashok Samal, George E. Meyer

Biological Systems Engineering: Papers and Publications

Beef tenderness is an important quality attribute for consumer satisfaction. The current beef quality grading system does not incorporate a direct measure of tenderness because there is currently no accurate, rapid, nondestructive method for predicting tenderness available to the beef industry. The objective of this study was to develop and test a visible/nearinfrared hyperspectral imaging system to predict tenderness of 14-day aged, cooked beef from hyperspectral images of fresh ribeye steaks acquired at 14-day post-mortem. A push-broom hyperspectral imaging system (wavelength range: 400-1000 nm) with a diffuse-flood lighting system was developed and calibrated. Hyperspectral images of beef-steak (n = 111) …

Construction, Installation, And Performance Of Two Repacked Weighing Lysimeters, José O. Payero, Suat Irmak

Biological Systems Engineering: Papers and Publications

Weighing lysimeters are the standard method for directly measuring evapotranspiration (ET). This paper discusses the construction, installation, and performance of two (1.52 m × 1.52 m × 2.13-m deep) repacked weighing lysimeters for measuring ET of corn and soybean in West Central Nebraska. The cost of constructing and installing each lysimeter was approximately US $12,500, which could vary depending on the availability and cost of equipment and labor. The resolution of the lysimeters was 0.0001 mV V^-1, which was limited by the data processing and storage resolution of the datalogger. This resolution was equivalent to 0.064 and 0.078 …

Pathogen Best Management Practices (Bmps), Richard K. Koelsch

Department of Biological Systems Engineering: Conference Presentations and White Papers

Management practices targeting pathogens focus on establishing multiple barriers between the pathogen source and water, with each barrier designed to lessen the risk of pathogens. The “Agricultural Environmental Management” program developed at Cornell University for dairy producers in New York watersheds promotes a four-barrier approach.

High Temperature Dilute Acid Pretreatment Of Coastal Bermudagrass, Arthur Redding, Deepak R. Keshwani, Ziyu Wang, Jay J. Cheng

Department of Biological Systems Engineering: Conference Presentations and White Papers

The conversion of lignocellulosic biomass into ethanol is an encouraging technology in the face of concerns over global warming and finite energy resources. In the southeastern United States, coastal bermudagrass shows potential for use as an energy crop for ethanol production. A review of the literature has shown that research has been done on the dilute sulfuric acid pretreatment of costal bermudagrass at 121ºC prior to enzymatic hydrolysis. This study examined dilute acid concentrations of 0.3%(w/w) to 1.2%(w/w) at temperatures from 120C to 180C over residence times of 5 to 60 minutes in an effort to optimize the pretreatment process …