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Full-Text Articles in Biological Engineering
Extracellular Matrix Protein Orientation By Adsorption On Self-Assembled Monolayers Controls Nonviral Gene Delivery, Beth A. Duensing, Angela K. Pannier
Extracellular Matrix Protein Orientation By Adsorption On Self-Assembled Monolayers Controls Nonviral Gene Delivery, Beth A. Duensing, Angela K. Pannier
Department of Biological Systems Engineering: Conference Presentations and White Papers
To date, most efforts to understand and improve the efficiency of nonviral gene delivery have focused on altering the physicochemical properties of delivery systems and developing new delivery strategies. The importance of the cellular microenvironment in achieving successful nonviral gene transfer has not been thoroughly examined, though proteins of the extracellular matrix (ECM) mediate cell adhesion and serve to regulate cellular behavior that may, in turn, dictate the cellular responsiveness to nonviral gene delivery. Self-assembled monolayers (SAMs) of alkanethiols on gold were used to control adsorption of ECM molecules and thus control the architecture of the extracellular environment presented to …
Effects Of Narrow Grass Hedges On Nutrient Transport From Land Application Areas, John E. Gilley, Bahman Eghball, David B. Marx
Effects Of Narrow Grass Hedges On Nutrient Transport From Land Application Areas, John E. Gilley, Bahman Eghball, David B. Marx
Department of Biological Systems Engineering: Conference Presentations and White Papers
The placement of stiff-stemmed grass hedges on the contour along a hill slope has been shown to decrease runoff nutrient transport. This study was conducted to measure the effectiveness of a narrow grass hedge in reducing runoff nutrient transport from plots with a range of soil nutrient values. Composted beef cattle manure was applied at dry weights of 0, 68, 105, 142, and 178 Mg ha-1 to a silty clay loam soil and then incorporated by disking. Soil samples were collected 243 days later for analysis of water-soluble phosphorus (WSP), Bray and Kurtz No.1 phosphorus (Bray-1 P), NO3 …
Nutrient And Microbial Transport From Feedlot Surfaces, John E. Gilley, Elaine D. Berry, Roger A. Eigenberg, David B. Marx, Bryan L. Woodbury
Nutrient And Microbial Transport From Feedlot Surfaces, John E. Gilley, Elaine D. Berry, Roger A. Eigenberg, David B. Marx, Bryan L. Woodbury
Department of Biological Systems Engineering: Conference Presentations and White Papers
Nutrient and microbial transport by runoff may vary at different locations within a beef cattle feedlot. If the areas making the largest contributions to nutrient and microbial transport can be identified, it may be possible to institute site-specific management practices to reduce runoff nutrient and microbial transport. The objectives of this study were to: a) measure selected feedlot soil properties, and nutrient and microbial transport in runoff from various feedlot locations b) compare the effects of unconsolidated surface materials (USM) (loose manure pack) and consolidated subsurface materials (CSM) (compacted manure and underlying layers) on nutrient and microbial transport, and c) …
Pathogen Best Management Practices (Bmps), Richard K. Koelsch
Pathogen Best Management Practices (Bmps), Richard K. Koelsch
Department of Biological Systems Engineering: Conference Presentations and White Papers
Management practices targeting pathogens focus on establishing multiple barriers between the pathogen source and water, with each barrier designed to lessen the risk of pathogens. The “Agricultural Environmental Management” program developed at Cornell University for dairy producers in New York watersheds promotes a four-barrier approach.
High Temperature Dilute Acid Pretreatment Of Coastal Bermudagrass, Arthur Redding, Deepak R. Keshwani, Ziyu Wang, Jay J. Cheng
High Temperature Dilute Acid Pretreatment Of Coastal Bermudagrass, Arthur Redding, Deepak R. Keshwani, Ziyu Wang, Jay J. Cheng
Department of Biological Systems Engineering: Conference Presentations and White Papers
The conversion of lignocellulosic biomass into ethanol is an encouraging technology in the face of concerns over global warming and finite energy resources. In the southeastern United States, coastal bermudagrass shows potential for use as an energy crop for ethanol production. A review of the literature has shown that research has been done on the dilute sulfuric acid pretreatment of costal bermudagrass at 121ºC prior to enzymatic hydrolysis. This study examined dilute acid concentrations of 0.3%(w/w) to 1.2%(w/w) at temperatures from 120C to 180C over residence times of 5 to 60 minutes in an effort to optimize the pretreatment process …
Alkaline Pretreatment Of Coastal Bermudagrass For Bioethanol Production, Ziyu Wang, Deepak R. Keshwani, Arthur P. Redding, Jay J. Cheng
Alkaline Pretreatment Of Coastal Bermudagrass For Bioethanol Production, Ziyu Wang, Deepak R. Keshwani, Arthur P. Redding, Jay J. Cheng
Department of Biological Systems Engineering: Conference Presentations and White Papers
Lignocellulosic materials are regarded as an alternative energy source for bioethanol production to reduce our reliance on fossil fuels. Pretreatment is important for improving the enzymatic digestibility of lignocelluloses to increase the yield of fermentable sugars. Alkaline (sodium hydroxide and lime (calcium hydroxide)) pretreatment of coastal bermudagrass for enhanced reducing sugars recovery was investigated in this study. The effect of NaOH pretreatment at 121°C using 1%, 2% and 3% (w/v) NaOH for 15, 30, 60 and 90 minutes was evaluated first. Lower NaOH concentrations (0.5% and 0.75%) and lower temperatures (50, 80 and 100°C) were then examined. Lime (0.1 g …