Biological Engineering Commons^™

Ultrasonic Concentration Of Microorganisms, Samuel J. Mullins

Theses and Dissertations--Biosystems and Agricultural Engineering

Concentration of microorganisms from a sample volume would increase the limits of detection of samples used for rapid-detection methods. Rapid detection methods are is advantageous for the food industry to rapidly test for bacteria in order release products on a timely basis. Ultrasonic concentration was considered a promising method for manipulation of microorganisms. An ultrasonic chamber consisting of parallel piezoceramic discs with a reticulated polyurethane foam mesh was used to concentrate Saccharomyces cerevisiae yeast and Escherichia coli bacteria. The concentration of yeast was seen to increase by 200% (from 8.0 x 10⁴ cells mL^-1 to 2.4 x 10 …