Biological Engineering Commons^™

Department Of Biological Systems Engineering Newsletter, Issue 3, Vol. 2, December 2007

BSE Department Magazine

Contents:
New Tractor Test Track Dedication
From the Department Head
Awards
Meet the Faculty
Alumni News
Student News
Summer Graduations
Quarter-scale Tractor Competition
ASABE Student Branch
ASABE International Preprofessional Council
Distinguished Fellowship Awards
John and Louise Skala Fellowships
Researching Plant Sterols
Milton E. Mohr Fellowship in Agriculture
Scholarships and Ice Cream
100 YEARS As a University of Nebraska Department
Alumnus Profile
Career opportunities in food engineering
Congratulations
Comings and Goings

Transfected Cell Arrays For The High-Throughput Analysis Of Transcription Factor Activity, Abigail D. Bellis, Angela K. Pannier, Lonnie D Shea

Department of Biological Systems Engineering: Conference Presentations and White Papers

Transfected cell arrays offer a high-throughput method that allows for the parallel analysis of multiple pathways or genes within a physiological context. We have developed a transfected cell array that employs a dual-plasmid system that gives the ability to normalize for spot-to-spot variation in transfection efficiency. Each spot within the array contains a constitutively active normalization plasmid encoding for renilla luciferase as well as a functional plasmid that contains transcription factor specific binding elements driving the expression of firefly luciferase. Dual bioluminescent imaging provides highly sensitive analysis while limiting post-transfectional processing. Adequate transfection poses a significant challenge in extending this …

Bioluminescence Imaging For Assessment And Normalization In Transfected Cell Arrays, Angela K. Pannier, Eric A. Ariazi, Abigail D. Bellis, Zain Bengali, V. Craig Jordan, Lonnie D. Shea

Biological Systems Engineering: Papers and Publications

Transfected cell arrays (TCAs) represent a high-throughput technique to correlate gene expression with functional cell responses. Despite advances in TCAs, improvements are needed for the widespread application of this technology. We have developed a TCA that combines a two-plasmid system and dual-bioluminescence imaging to quantitatively normalize for variability in transfection and increase sensitivity. The two-plasmids consist of: (i) normalization plasmid present within each spot, and (ii) functional plasmid that varies between spots, responsible for the functional endpoint of the array. Bioluminescence imaging of dual-luciferase reporters (renilla, firefly luciferase) provides sensitive and quantitative detection of cellular response, with minimal post-transfection processing. …

Association Of Odor Measures With Annoyance: An Odor-Monitoring Field Study, Richard R. Stowell, Christopher G. Henry, Richard K. Koelsch, Dennis D. Schulte

Department of Biological Systems Engineering: Conference Presentations and White Papers

Multiple assessments of ambient odor were made by trained individuals in the vicinity of a swine finishing operation in eastern Nebraska during the summers of 2005 and 2006. This paper addresses an analysis of assessor responses in Year 1 of this field study to determine what relationships existed between field odor measurements/ratings and ratings of annoyance potential, and to identify candidate measurement threshold values for odors that are likely to cause an annoyance. The first-year results showed that the likelihood of odor causing annoyance increased as ambient odors became more offensive, more intense, and more concentrated, with r² values …

National Livestock And Poultry Environmental Learning Center, Richard K. Koelsch, Mark Risse, Joe Harrison, Jill Heemstra

Department of Biological Systems Engineering: Conference Presentations and White Papers

This paper describes a new national learning center designed to improve the delivery of science-based information to non-research customers. The vision of the Livestock and Poultry Environmental Learning Center is to provide individuals involved in public policy issues, animal production, and delivery of technical services for confined animal systems with on-demand access to the nation’s best science-based resources that is responsive to priority and emerging environmental issues associated with animal agriculture. This project will test innovative approaches such as a web cast seminar series to connect national experts with those individuals and organizations that influence animal producer decisions on manure …

Software Tool For Integrating Feed Management Into Nutrient Planning, Richard K. Koelsch, Ray Massey, Galen E. Erickson, Virgil R. Bremer

Department of Biological Systems Engineering: Conference Presentations and White Papers

The introduction of ASABE Standard D384.2, Manure Production and Characteristics, has created the opportunity to integrate feed management decisions and animal performance measures into nutrient planning processes. This paper introduces a software tool that integrates estimates of nutrient excretion based on the new standard with estimates of land need, labor and equipment time allocation, and economic cost and benefit for manure application. This tool will be used to evaluate the economic implications for two scenarios using beef cattle examples as a means of illustrating tool application. The first scenario will look at the impact of feeding ethanol co-products on the …

Nitrogen And Phosphorus Concentrations Of Runoff As Affected By Moldboard Plowing, John E. Gilley, Bahman Eghball, David B. Marx

Biological Systems Engineering: Papers and Publications

The excessive application of manure on cropland areas can cause nutrients to accumulate near the soil surface. This study was conducted to measure the effects of moldboard plowing on the redistribution of nutrients within the soil profile and nutrient transport by overland flow. Composted beef cattle manure was applied at dry weights of 0, 68, 105, 142, and 178 Mg ha^-1 to a silty clay loam soil and then incorporated by disking. Selected plots were moldboard plowed 244 days later to a depth of approximately 23 cm. Soil samples for analysis of water‐soluble phosphorus, Bray and Kurtz No. 1 …

Nutrient Concentrations Of Runoff During The Year Following Manure Application, John E. Gilley, Bahman Eghball, D. B. Marx

Biological Systems Engineering: Papers and Publications

Little information is currently available concerning temporal changes in nutrient transport following the addition of manure to cropland areas. This study was conducted to measure nutrient transport in runoff as affected by tillage and time following the application of beef cattle or swine manure to a site on which corn (Zea mays L.) was grown. Rainfall simulation tests were initiated 4, 32, 62, 123, and 354 days following land application. Three 30 min simulated rainfall events, separated by 24 h intervals, were conducted at an intensity of approximately 70 mm h-1. Dissolved phosphorus (DP), particulate phosphorus (PP), total phosphorus …

Water Erosion Prediction Project (Wepp): Development History, Model Capabilities, And Future Enhancements, Dennis C. Flanagan, John E. Gilley, Thomas G. Franti

Biological Systems Engineering: Papers and Publications

The Water Erosion Prediction Project (WEPP) was initiated in August 1985 to develop new‐generation water erosion prediction technology for use by federal action agencies involved in soil and water conservation and environmental planning and assessment. Developed by the USDA‐ARS as a replacement for empirically based erosion prediction technologies, the WEPP model simulates many of the physical processes important in soil erosion, including infiltration, runoff, raindrop and flow detachment, sediment transport, deposition, plant growth, and residue decomposition. The WEPP project included an extensive field experimental program conducted on cropland, rangeland, and disturbed forest sites to obtain data required to parameterize and …

Microwave Pretreatment Of Switchgrass To Enhance Enzymatic Hydrolysis, Deepak R. Keshwani, Jay J. Cheng, Joseph C. Burns, Laigeng Li, Vincent Chiang

Department of Biological Systems Engineering: Conference Presentations and White Papers

Switchgrass is a promising lignocellulosic biomass for fuel-ethanol production. However, pretreatment of lignocellulosic materials is necessary to improve its susceptibility to enzymatic hydrolysis. The objectives of this study were to examine the feasibility of microwave pretreatment to enhance enzymatic hydrolysis of switchgrass and to determine the optimal pretreatment conditions. Switchgrass samples immersed in water, dilute sulfuric acid and dilute sodium hydroxide solutions were exposed to microwave radiation at varying levels of radiation power and residence time. Pretreated solids were enzymatically hydrolyzed and reducing sugars in the hydrolysate were analyzed. Microwave radiation of switchgrass at lower power levels resulted in more …

Moldboard Plowing Following Compost Application Significantly Reduces Nutrient Transport, John E. Gilley, Bahman Eghball, David B. Marx

Department of Biological Systems Engineering: Conference Presentations and White Papers

The excessive application of manure on cropland areas can cause nutrients to accumulate near the soil surface and increase nutrient transport by overland flow. Inverting soils with high surface nutrient content could reduce runoff nutrient transport. This study was conducted to measure the effects of moldboard plowing on the redistribution of nutrients within the soil profile and nutrient transport by overland flow. Composted beef cattle manure was applied at dry weights of 0, 68, 105, 142, and 178 Mg ha^-1 to a silty clay loam soil and then incorporated by disking. Selected plots were moldboard plowed 244 days later …

Runoff Nutrient Concentrations Following Manure Application As Affected By Crop Residue, John E. Gilley, Jeffrey Nicolaisen, Eghball Bahman, David B. Marx

Department of Biological Systems Engineering: Conference Presentations and White Papers

Manure is applied to cropland areas managed under diverse conditions resulting in varying amounts of residue cover. The objective of this study was to measure the effects of crop residue on nutrient concentrations in runoff from areas where beef cattle or swine manure were recently applied but not incorporated. Plots 0.75 m wide by 2 m long were established at the study site. Existing residue materials were removed, and corn, soybean, or winter wheat residue was added at rates of 2, 4, or 8 Mg ha-1. Manure was applied at rates required to meet estimated annual nitrogen requirements for corn. …

Temporal Changes In Nutrient Transport Following Land Application Of Manure, John E. Gilley, Eghball Bahman, D. B. Marx

Department of Biological Systems Engineering: Conference Presentations and White Papers

Little information is currently available concerning temporal changes in nutrient transport following the addition of manure to cropland areas. This study was conducted to measure nutrient transport in runoff as affected by tillage and time following the application of beef cattle or swine manure to a site on which corn [Zea mays (L.)] was grown. Rainfall simulation tests were initiated 4, 32, 62, 123, and 354 days following land application. Three 30-min simulated rainfall events, separated by 24-hour intervals, were conducted at an intensity of approximately 70 mm hr-1. Dissolved phosphorus (DP), particulate phosphorus (PP), total phosphorus (TP), NO3-N, …

Effect Of Overland Flow Rate On Nutrient Transport From Land Application Areas, John E. Gilley, William F. Sabatka, Bahman Eghball, David B. Marx

Department of Biological Systems Engineering: Conference Presentations and White Papers

Little information is currently available concerning the effects of varying flow rate on nutrient transport by overland flow. The objective of this study was to measure the effects of overland flow rate on nutrient transport following the application of beef cattle or swine manure to plots containing 0, 2, 4, or 8 Mg ha^-1 of corn residue. After addition of residue materials to 0.75 m wide x 2.0 m long plots, beef cattle or swine manure was added and the plots were then either disked or maintained in a no-till condition. Three 30-min simulated rainfall events, separated by 24-hour …

Department Of Biological Systems Engineering Newsletter, Vol. 3, Issue 1, June 2007

BSE Department Magazine

Contents:
Award Winning Academic Advisor
Hall of Fame 2007 Leslie W. Jochens, P.E. Leader in the Irrigation Industry
From the Department Head
John Deere Dealership Management Banquet
Alumni Profiles: Mike Henry
The Incredible, Edible Vehicle Competition
Graduation
December 2006 Graduates
May 2007 Graduates
Undergraduate Creative Activities and Research Experiences—UCARE
Honors Convocation
In Memoriam
Alumni News
Faculty News
Comings and Goings

Co-Production Of Chemical And Energy Products From Distillers Grains Using Supercritical Fluid Extraction And Thermochemical Conversion Technologies, Lijun Wang, Ajay Kumar, Curtis L. Weller, David D. Jones, Milford A. Hanna

Department of Biological Systems Engineering: Conference Presentations and White Papers

Dry-grind technology has become the dominant method for ethanol production. During drygrind ethanol production, roughly one-third of the dry grain mass resides in the dried distillers grains with solubles (DDGS) as by-products (i.e., ∼0.8 kg/L ethanol). The energy content residing in the 0.8 kg DDGS is about 20 MJ, compared to the consumption of 1 MJ electricity and 10 MJ thermal energy for production of each liter of ethanol. A sequential supercritical fluid process with solvents including carbon dioxide, water and ethanol was used to recover high-value chemicals from DDGS. Thermochemical conversion methods were used to convert the DDGS residue …

Early Investment In Soil Conservation Research Continues To Provide Dividends, John E. Gilley, D. C. Flanagan

Biological Systems Engineering: Papers and Publications

Current soil conservation programs are built upon an established research legacy. Hugh H. Bennett, chief of the USDA Soil Conservation Service from 1935 to 1951, was instrumental in the establishment of a network of 35 soil conservation experiment stations (SCES). Research projects were initiated at the SCES in the 1930s to investigate the principal factors causing erosion and to identify the most effective and practical methods of controlling soil loss from agricultural areas. Information obtained from the SCES, and selected other locations, was assembled at the National Runoff and Soil Loss Data Center (NRSLDC) established on the campus of Purdue …

Characteristics Of Manure Harvested From Beef Cattle Feedlots, William F. Kissinger, Richard K. Koelsch, Galen E. Erickson, Terry J. Klopfenstein

Biological Systems Engineering: Papers and Publications

Open lot cattle production systems present unique challenges for nutrient planning processes. Previous estimates of quantities and characteristics of harvested manure from this type of facility are based upon data from the early 1970s. In addition, harvested manure is impacted by weather, feeding program, season, and pen management decisions. The objectives of this study are to characterize under commercial conditions for open lot beef systems: 1) harvested manure quantities and characteristics; 2) impact of factors such as feeding program, season, and management on harvested manure; and 3) mass balance for nitrogen (N) and phosphorus (P). Data from six commercial feedlots …

Potential Of Agricultural Residues And Hay For Bioethanol Production, Ye Chen, Ratna R. Sharma-Shivappa, Deepak R. Keshwani, Chengci Chen

Biological Systems Engineering: Papers and Publications

Production of bioethanol from agricultural residues and hays (wheat, barley, and triticale straws, and barley, triticale, pearl millet, and sweet sorghum hays) through a series of chemical pretreatment, enzymatic hydrolysis, and fermentation processes was investigated in this study. Composition analysis suggested that the agricultural straws and hays studied contained approximately 28.62–38.58% glucan, 11.19–20.78% xylan, and 22.01–27.57% lignin, making them good candidates for bioethanol production. Chemical pretreatment with sulfuric acid or sodium hydroxide at concentrations of 0.5, 1.0, and 2.0% indicated that concentration and treatment agent play a significant role during pretreatment. After 2.0% sulfuric acid pretreatment at 121°C/15 psi for …

Crop Residue Effects On Runoff Nutrient Concentrations Following Manure Application, Jeffrey E. Nicolaisen, John E. Gilley, Bahman Eghball, David B. Marx

Biological Systems Engineering: Papers and Publications

Manure is applied to cropland areas managed under diverse conditions, resulting in varying amounts of residue cover. The objective of this study was to measure the effects of crop residue on nutrient concentrations in runoff from areas where beef cattle or swine manure were recently applied but not incorporated. Plots 0.75 m wide by 2 m long were established at the study site. Existing residue materials were removed, and corn, soybean, or winter wheat residue was added at rates of 2, 4, or 8 Mg ha⁻¹. Manure was then applied at rates required to meet estimated annual nitrogen …

Energy And Environmental Contributions Of Corn-Ethanol, Adam Liska, Kenneth Cassman, Haishun Yang

Adam Liska Papers

Rapid development of regulatory mechanisms to mitigate greenhouse gas (GHG) emissions requires that the biofuel industry employ standard methods to evaluate biofuel systems comprising both crop production systems and biorefineries. Biofuel systems associated with a variety of organic feedstocks have a range of performance capabilities, and recent life-cycle assessment studies of these different systems have used inconsistent methods, leading to confusion about biofuel energy efficiency and GHG mitigation. Hence, there is a critical need for well documented life-cycle metrics for consistent biofuel evaluation that are established and supported by a national or international governing body. Standardization of net energy and …

A “Sprinkler” Vegetative Treatment System, Jason Gross, Chris G. Henry

Department of Biological Systems Engineering: Conference Presentations and White Papers

Vegetative Treatment Systems have historically been a practice for the treatment and utilization of open lot livestock wastes. Use of these systems has been rejuvenated in recent years. A “sprinkler” vegetative treatment area (VTA) has been designed and developed as a proof of concept for addressing challenges experienced with small and medium Animal Feeding Operations. The focus of this paper is to report on the design, economics, challenges and the lessons learned on the development of this system. Cost data shows the cost of a “sprinkler” VTS to range between $31 to $63/head, compared to $51 to $170/head for a …

Sharing Distance Education Courses Among Universities, Leon Schmacher, Thomas Brumm, John Slocombe, Jack L. Schinstock

Department of Biological Systems Engineering: Conference Presentations and White Papers

Four universities collaborated to develop mechanized systems management (agricultural systems management, agricultural systems technology, etc.) courses to be shared via distance education. Funded by a USDA Higher Education Challenge Grant, these undergraduate and graduate courses were initially offered to students at Iowa State University, Kansas State University, the University of Missouri, and the University of Nebraska-Lincoln. This effort is affiliated with the Great Plains Interactive Distance Education Alliance (Great Plains IDEA).

The objectives of this cooperative effort were to: (1) develop a collaborative, multi-state educational effort to add depth and breadth to each university’s instructional program in mechanized systems management; …