Open Access. Powered by Scholars. Published by Universities.®
- Institution
- Keyword
-
- 4-β-xylanase (1)
- Anaerobic (1)
- Bacteria (1)
- Bioenergy (1)
- Biotechnology (1)
-
- Cellulolytic (1)
- Cellulosome (1)
- Characterization (1)
- Clostridium thermocellum (1)
- Deletion (1)
- Endo-1 (1)
- Enzyme (1)
- Gene (1)
- Genetic engineering; college student viewpoints on genetic engineering; college students; assisted reproductive technology; mass media; ethics; Rhode Island College; Weber’s value-rational action theory; Bourdieu’s theory of capital; (1)
- Glycosidases (1)
- Glycosylases (1)
- Gram positive (1)
- Hydrolases (1)
- Lignocellulose utilization (1)
- Plasmid (1)
- Public policy (1)
- Rolling circle (1)
- Science (1)
- Social research (1)
- Sociology (1)
- Target (1)
- Targeting (1)
- Temperature sensitive (1)
- Thermoanaerobacterium (1)
- Thermophile (1)
- Publication
Articles 1 - 4 of 4
Full-Text Articles in Biological Engineering
Characterization Of Xylan Utilization And Discovery Of A New Endoxylanase In Thermoanaerobacterium Saccharolyticum Through Targeted Gene Deletions, Kara K. Podkaminer, Adam M. Guss, Heather L. Trajano, David A. Hogsett, Lee R. Lynd
Characterization Of Xylan Utilization And Discovery Of A New Endoxylanase In Thermoanaerobacterium Saccharolyticum Through Targeted Gene Deletions, Kara K. Podkaminer, Adam M. Guss, Heather L. Trajano, David A. Hogsett, Lee R. Lynd
Dartmouth Scholarship
The economical production of fuels and commodity chemicals from lignocellulose requires the utilization of both the cellulose and hemicellulose fractions. Xylanase enzymes allow greater utilization of hemicellulose while also increasing cellulose hydrolysis. Recent metabolic engineering efforts have resulted in a strain of Thermoanaerobacterium saccharolyticum that can convert C5 and C6 sugars, as well as insoluble xylan, into ethanol at high yield. To better understand the process of xylan solubilization in this organism, a series of targeted deletions were constructed in the homoethanologenic T. saccharolyticum strain M0355 to characterize xylan hydrolysis and xylose utilization in this organism. While the deletion of …
Computational Design And Characterization Of A Temperature-Sensitive Plasmid Replicon For Gram Positive Thermophiles, Daniel G. Olson, Lee R. Lynd
Computational Design And Characterization Of A Temperature-Sensitive Plasmid Replicon For Gram Positive Thermophiles, Daniel G. Olson, Lee R. Lynd
Dartmouth Scholarship
Temperature-sensitive (Ts) plasmids are useful tools for genetic engineering, but there are currently none compatible with the gram positive, thermophilic, obligate anaerobe, Clostridium thermocellum. Traditional mutagenesis techniques yield Ts mutants at a low frequency, and therefore requires the development of high-throughput screening protocols, which are also not available for this organism. Recently there has been progress in the development of computer algorithms which can predict Ts mutations. Most plasmids currently used for genetic modification of C. thermocellum are based on the replicon of plasmid pNW33N, which replicates using the RepB replication protein. To address this problem, we set out …
Living In An Age Of Technology: College Students' Perception Of The Role Of Genetic Engineering And Reproductive Technology In Today's Society, Kayla Quadros
Honors Projects
This study examines attitudes about prevalent issues in genetics and reproduction among the college population. Eighteen interviews were conducted with students at Rhode Island College. Respondents were asked questions about their moral standpoints concerning utilizing genetic engineering for disease control and aesthetic purposes, employing assisted reproductive technologies (ARTs), regulation of such procedures, and also about media sources for which they have heard of these topics. Findings suggest that, generally, participants felt that genetic engineering for health issues is permissible, yet reprehensible for the purpose of aesthetically "designing" a baby, though four students (three of which were female) found the enhancement …
Complete Genome Sequence Of Clostridium Clariflavum Dsm 19732, Javier A. Izquierdo, Lynne Goodwin, Karen W. Davenport, Hazuki Teshima
Complete Genome Sequence Of Clostridium Clariflavum Dsm 19732, Javier A. Izquierdo, Lynne Goodwin, Karen W. Davenport, Hazuki Teshima
Dartmouth Scholarship
Clostridium clariflavum is a Cluster III Clostridium within the family Clostridiaceae isolated from thermophilic anaerobic sludge (Shiratori et al, 2009). This species is of interest because of its similarity to the model cellulolytic organism Clostridium thermocellum and for the ability of environmental isolates to break down cellulose and hemicellulose. Here we describe features of the 4,897,678 bp long genome and its annotation, consisting of 4,131 protein-coding and 98 RNA genes, for the type strain DSM 19732.