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2022

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Full-Text Articles in Engineering

Mechanics Modeling Of Non-Rigid Origami: From Qualitative To Quantitative Accuracy, Jiayue Tao Dec 2022

Mechanics Modeling Of Non-Rigid Origami: From Qualitative To Quantitative Accuracy, Jiayue Tao

All Dissertations

Origami, the ancient art of paper folding, has recently evolved into a design and fabrication framework for various engineering systems at vastly different scales: from large-scale deployable airframes to mesoscale biomedical devices to small-scale DNA machines. The increasingly diverse applications of origami require a better understanding of the fundamental mechanics and dynamics induced by folding. Therefore, formulating a high-fidelity simulation model for origami is crucial, especially when large amplitude deformation/rotation exists during folding.

The currently available origami simulation models can be categorized into three branches: rigid-facet models, bar-hinge models, and finite element models. The first branch of models assumes that …


Multi-Criteria Performance Evaluation And Control In Power And Energy Systems, Payam Ramezani Badr Dec 2022

Multi-Criteria Performance Evaluation And Control In Power And Energy Systems, Payam Ramezani Badr

All Dissertations

The role of intuition and human preferences are often overlooked in autonomous control of power and energy systems. However, the growing operational diversity of many systems such as microgrids, electric/hybrid-electric vehicles and maritime vessels has created a need for more flexible control and optimization methods. In order to develop such flexible control methods, the role of human decision makers and their desired performance metrics must be studied in power and energy systems. This dissertation investigates the concept of multi-criteria decision making as a gateway to integrate human decision makers and their opinions into complex mathematical control laws. There are two …


Implementation Of Sic Power Electronics For Green Energy Based Electrification Of Transportation, Naireeta Deb Dec 2022

Implementation Of Sic Power Electronics For Green Energy Based Electrification Of Transportation, Naireeta Deb

All Dissertations

Increase in greenhouse gas emission poses a threat to the quality of air thus threatening the future of living beings on earth. A large part of the emission is produced by transport vehicles. Electric vehicles (EVs) are a great solution to this threat. They will completely replace the high usage of hydrocarbons in the transport sector. Energy efficiency and reduced local pollution can also be expected with full implementation of electrification of transportation. However, the current grid is not prepared to take the power load of EV charging if it were to happen readily. Moreover, critics are doubtful about the …


Multiple Heat Exchanger Cooling System For Automotive Applications – Design, Mathematical Modeling, And Experimental Observations, Zaker Syed Dec 2022

Multiple Heat Exchanger Cooling System For Automotive Applications – Design, Mathematical Modeling, And Experimental Observations, Zaker Syed

All Dissertations

The design of the automotive cooling systems has slowly evolved from engine-driven mechanical to computer-controlled electro-mechanical components. With the addition of computer-controlled variable speed actuators, cooling system architectures have been updated to maximize performance and efficiency. By switching from one large radiator to multiple smaller radiators with individual flow control valves, the heat rejection requirements may be precisely adjusted. The combination of computer regulated thermal management system should reduce power consumption while satisfying temperature control objectives. This research focuses on developing and analyzing a multi-radiator system architecture for implementation in ground transportation applications. The premise is to use a single …


Infusing Kirigami Principles Into Design Of Mechanical Properties, Hesameddin Khosravi Dec 2022

Infusing Kirigami Principles Into Design Of Mechanical Properties, Hesameddin Khosravi

All Dissertations

The emergence of mechanical metamaterials — which derive their properties primarily from the underlying architecture rather than the constituent material — has unleashed a new era of material design and functionalities. To fully materialize the promising potentials of metamaterials, it is crucial to develop versatile, scalable, and easy-to-fabricate methods that can both generate and tailor the underlying periodic architecture. To this end, we propose the use of kirigami — a popular recreational art of cutting and manipulating paper — as a platform to create periodicity and super-stretchability. Kirigami has become a design and fabrication framework for constructing metamaterials, robotic tools, …


Life Cycle Assessment Of Novel Electrochemical Struvite Recovery In Municipal Wastewater Treatment At The Plant And U.S. Watershed Scales, Karla Morrissey Dec 2022

Life Cycle Assessment Of Novel Electrochemical Struvite Recovery In Municipal Wastewater Treatment At The Plant And U.S. Watershed Scales, Karla Morrissey

Graduate Theses and Dissertations

As the supply of phosphorus decreases, nutrient recovery in municipal wastewater treatment has become an important area of research. Recovery methods for phosphorus-containing fertilizers, such as struvite, and other products are currently being developed and assessed for their efficiency and economic feasibility. Potential environmental impacts from nutrient recovery are also being assessed as design for the environment is important, especially with regards to the eutrophying potential of phosphorus in phosphorus-limited freshwater aquatic systems. Life cycle assessment (LCA) is a tool that can be used to estimate potential environmental impacts of a product or service focusing on its entire life cycle. …


A Multi-Criteria Ranking System For Prioritizing Maintenance Of Levee Systems In Arkansas, Nguyen Danh Phan Dec 2022

A Multi-Criteria Ranking System For Prioritizing Maintenance Of Levee Systems In Arkansas, Nguyen Danh Phan

Graduate Theses and Dissertations

There are 208,009 properties in Arkansas that have more than a 26% chance of being severely affected by flooding over the next 30 years, which represents 13% of all properties in the state. A levee system is designed to reduce the flooding risk for urban and rural communities; however, most of the state's levees have been significantly outdated or built with engineering standards less rigorous than current best practices. The Levee Safety Action Classification (LSAC), as recorded in the National Levee Database (NLD), communicates the risk associated with living behind a particular levee and assists local, state, and federal stakeholders …


Surface-Driven Instabilities At Oil-Water Interfaces, Anelisse Claros Mendieta Dec 2022

Surface-Driven Instabilities At Oil-Water Interfaces, Anelisse Claros Mendieta

Graduate Theses and Dissertations

Fluid-fluid interactions are such a wide area and can be observed in different scenarios such as geothermal systems, waterflooding of reservoirs, oil recovery, and water treatment processes. This thesis aims to deepen the understanding of fluid-fluid interactions which include Marangoni flows, charge related instabilities, and surface activity. This thesis studied the interactions of micrometer-sized droplets of an oil (toluene) with surfactant Di-(2-ethylhexyl) phosphoric acid (DEHPA) in the aqueous phase. One of the main goals is to understand several surface-driven instabilities and how those can be manipulated for future applications.Furthermore, this thesis aims to use these oil-surfactant droplets as a medium …


Hierarchical Structure And Material Integration For Electrocatalytic Co2 Reduction, Hamed Mehrabi Dec 2022

Hierarchical Structure And Material Integration For Electrocatalytic Co2 Reduction, Hamed Mehrabi

Graduate Theses and Dissertations

CO2 released by the combustion of fossil fuels is driving significant changes to the earth’sclimate. The natural cycle for removing CO2 from the atmosphere, namely photosynthesis, cannot keep up with the rate at which it is being added. Developing engineering approaches to remove CO2 from the atmosphere is becoming essential to reduce these effects. Removal leads to further issues of carbon sequestration and favorable CO2 reuse strategies, including the electrochemical transformation of recovered CO2 to useful products such as fuels and materials. Copper is an important electrocatalyst for the CO2 reduction reaction (CO2RR) because of its unique capability for producing …


Improved Compact Modeling Of High Voltage Gallium Nitride High Electron Mobility Transistors, Md Maksudul Hossain Dec 2022

Improved Compact Modeling Of High Voltage Gallium Nitride High Electron Mobility Transistors, Md Maksudul Hossain

Graduate Theses and Dissertations

Since power converters shape a wide range of power electronic applications, the 'heart' of these conversions, such as solid-state switches, is crucial for achieving higher power density, lower loss, and higher efficiency operations. Although the limitations imposed by silicon-based power devices have been reduced by introducing novel devices such as IGBTs and super junction MOSFETs to allow for broader applications, solutions beyond silicon have presented a continuous challenge to the device engineers. Driving these converters at a higher switching frequency allows for higher power density. However, to support a higher frequency and operating temperature, superior materials, e.g., wide bandgap materials, …


A Study On Interactions Between Metal-Organic Frameworks And Biological Materials, Josh Phipps Dec 2022

A Study On Interactions Between Metal-Organic Frameworks And Biological Materials, Josh Phipps

Graduate Theses and Dissertations

Metal-organic frameworks or MOFs are an extremely useful tool in many areas of applications. Their popularity in recent years has arisen from their high efficiency in catalytic chemical reactions. This is made possible due to their porous interior and the ability of the MOFs components to be functionalized. These same traits make MOFs excellent for use in protein encapsulation or immobilization and have the potential to become excellent drug carriers. Their development in this utilization has been limited dramatically compared to MOFs chemical applications. This is due in part to the nature of biological processes taking longer to study, but …


Using Copper Oxide And Zwitterions To Prevent Membrane Fouling And Optimizing Ultrafiltration Membrane Fabrication With Green Solvents, Cannon Hackett Dec 2022

Using Copper Oxide And Zwitterions To Prevent Membrane Fouling And Optimizing Ultrafiltration Membrane Fabrication With Green Solvents, Cannon Hackett

Graduate Theses and Dissertations

Membrane fouling and sustainable production of membranes are two of the most important challenges in membrane science. Chapter 1 of this dissertation introduces these challenges and covers previous research that has been done in those areas. Additionally, the objectives of this dissertation are stated. In chapter 2, a study was done in which polyethersulfone ultrafiltration membranes were functionalized with copper oxide nanoparticles and zwitterions for the purpose of reducing fouling on the membrane surface. Characterization confirmed the attachment of each component to the membranes. Dead-end filtration was performed with water and bovine serum albumin solution and showed that the functionalization …


Using Molecular Dynamics Simulations To Decipher Mechanistic Details Of Biomolecular Processes Of Biology And Biotechnology Oriented Applications, Adithya Polasa Dec 2022

Using Molecular Dynamics Simulations To Decipher Mechanistic Details Of Biomolecular Processes Of Biology And Biotechnology Oriented Applications, Adithya Polasa

Graduate Theses and Dissertations

Researchers in chemistry and biology often utilize computer simulations, in conjunction with experimental data, to model and predict the structures, energies, kinetics, processes, and functions of the systems that are their focus of study, ranging from single molecules to whole viruses. Here, we use molecular dynamics (MD) techniques to gain a deeper understanding of biomolecular processes in biology and biotechnology-oriented applications. Using a mixture of equilibrium and non-equilibrium MD simulations, this work describes the insertion process of YidC at the atomic level. In order to better comprehend the insertion process, several docking models of YidC-Pf3 in the lipid bilayer were …


Characterizing Climb And Propeller Performance For A Fixed Pitch, Single-Engine, Electric Aircraft, Austin Lawrence Andwan Dec 2022

Characterizing Climb And Propeller Performance For A Fixed Pitch, Single-Engine, Electric Aircraft, Austin Lawrence Andwan

Theses and Dissertations

Electric aircraft are on the horizon. Both industry and regulators have an abundance of flight tests ahead to ensure safe operation of these new aircraft. Conventional performance standards regaurding edurance, climb performance and cruise conditions are largely not applicable to assessing an electrically propulsed aircraft. While battery technologies are the current barrier for electric aircraft, there also hasn’t been much flight testing to reveal their unique performance trends. This experiment focus’s on characterizing some of these performance trends obtained through a level flight and climb test in the Pipistrel Velis Electro. The rate-of-climb did not exhibit a decrease when the …


Deep Learning Approach To Speech Recognition: A Signal Extractor & Producer For Artificial General Intelligence, Ahmad Zuhair S. Hasanain Dec 2022

Deep Learning Approach To Speech Recognition: A Signal Extractor & Producer For Artificial General Intelligence, Ahmad Zuhair S. Hasanain

Theses and Dissertations

The efficient use of a communication bandwidth starts with the data source. The features of the speech signals can be extracted and reconstructed to lower the Internet traffic of the acoustic artificial agents and to increase the quality of the automatic speech recognition systems. The Speech Quefrency Transform (SQT) is hereby introduced in the work to enrich the communication space between the artificial agents and mankind. We describe the motivation, methodology, and deep learning approach in detail as we apply the SQT technology to several applications: sharp pitch track extraction, real-time speech communications, and emotion recognition. The results were excellent. …


The Effect Of Zonation, Biofouling, And Corrosion Products On Cathodic Protection Design For Offshore Monopiles, Caglar Erdogan Dec 2022

The Effect Of Zonation, Biofouling, And Corrosion Products On Cathodic Protection Design For Offshore Monopiles, Caglar Erdogan

Theses and Dissertations

Cathodic protection (CP) is required to prevent corrosion of the submerged steel for offshore wind monopile foundations in the marine environment. The design of these systems depends on the environmental conditions, including temperature, salinity, pH, dissolved oxygen, depth, micro- and macro-organisms, and water flow rates. The standards utilized to design cathodic protection systems consider only some of these conditions. This research identified the gaps in knowledge for the design of corrosion prevention systems for offshore wind monopile foundations and investigated the changes in cathodic current densities due to macro-galvanic cells, fouling communities and corrosion products established on uncoated vertical steel …


Evaluation Of Metaverse Integration Of Freight Fluidity Measurement Alternatives Using Fuzzy Dombi Edas Model, Muhammet Deveci, Ilgin Gokasar, Oscar Castillo, Tugrul Daim Dec 2022

Evaluation Of Metaverse Integration Of Freight Fluidity Measurement Alternatives Using Fuzzy Dombi Edas Model, Muhammet Deveci, Ilgin Gokasar, Oscar Castillo, Tugrul Daim

Engineering and Technology Management Faculty Publications and Presentations

Developments in transportation systems, changes in consumerism trends, and conditions such as COVID-19 have increased both the demand and the load on freight transportation. Since various companies are transporting goods all over the world to evaluate the sustainability, speed, and resiliency of freight transportation systems, data and freight fluidity measurement systems are needed. In this study, an integrated decision-making model is proposed to advantage prioritize the freight fluidity measurement alternatives. The proposed model is composed of two main stages. In the first stage, the Dombi norms based Logarithmic Methodology of Additive Weights (LMAW) is used to find the weights of …


Ambient Air-Processed Cspbi3-Based Perovskite Solar Cell Via Semi-Sputtering Deposition And Numerical Studies Of Cs3sb2i9-Based Perovskite Solar Cell, Shamik Datta Dec 2022

Ambient Air-Processed Cspbi3-Based Perovskite Solar Cell Via Semi-Sputtering Deposition And Numerical Studies Of Cs3sb2i9-Based Perovskite Solar Cell, Shamik Datta

Theses and Dissertations

Commercialization of Perovskite solar cells as a clean energy source can augment the reduction of carbon footprint. CsPbI3, a well-known perovskite material, has been synthesized in ambient air and high-humidity conditions and utilized as the active layer in a perovskite solar cell. The rest of the solar cell layers have been sputter-deposited. This solar cell with a p-i-n configuration ITO/NiOx/CsPbI3/ZnO/Au exhibited a champion efficiency of 3.7%. Nonetheless, lead incorporation in the conventional Pb-cation-based perovskites poses an environmental threat. The physical proximity of living organisms to lead point sources exhibits a wide spectrum of negative consequences. In this study, we have …


Fostering Collaboration In Emerging Three-Tiered Spectrum Markets, Anindo Mahmood Dec 2022

Fostering Collaboration In Emerging Three-Tiered Spectrum Markets, Anindo Mahmood

Theses and Dissertations

Ensuring optimum spectral efficiency is a critical requirement for current wireless networks to cope with the ever-growing flow of wireless data traffic, using limited spectral resources. As such, spectrum sharing, which allows different grades of users, as well as multiple networking standards to co-exist and utilize in the same frequency band, has become a topic of great intrigue. Due to the inherent advantages of these schemes, the US government has opened up vast amounts of federal spectrum that supports spectrum sharing. The Citizens Broadband Radio Service (CBRS) proposed by the Federal Communications Commission (FCC) is one of them. A tiered …


Seeing The Big Picture: System Architecture Trends In Endoscopy And Led-Based Hyperspectral Subsystem Intergration, Craig M. Browning Dec 2022

Seeing The Big Picture: System Architecture Trends In Endoscopy And Led-Based Hyperspectral Subsystem Intergration, Craig M. Browning

Graduate Theses and Dissertations (2019 - present)

Early-stage colorectal lesions remain difficult to detect. Early development of neoplasia tends to be small (less than 10 mm) and flat and difficult to distinguish from surrounding mucosa. Additionally, optical diagnosis of neoplasia as benign or malignant is problematic. Low rates of detection of these lesions allow for continued growth in the colorectum and increased risk of cancer formation. Therefore, it is crucial to detect neoplasia and other non-neoplastic lesions to determine risk and guide future treatment. Technology for detection needs to enhance contrast of subtle tissue differences in the colorectum and track multiple biomarkers simultaneously. This work implements one …


Building A Frameworks For Apron Planning, Design, Optimization, Future Proofing And Expansion, Adam K. Wing Dec 2022

Building A Frameworks For Apron Planning, Design, Optimization, Future Proofing And Expansion, Adam K. Wing

Graduate Theses and Dissertations (2019 - present)

Airports are a significant economic driver that impact local and national interests. As such, in an ever connected world, these critical components of infrastructure face a growing number of influences which contribute to systems complexity and frequently impede further development. The point of this dissertation is to discuss and highlight the benefit of systematic thinking as planners approach airport planning challenges and update the aging aviation infrastructure in many regions of the world.

This dissertation looks at a series of three papers that, examine the impact and influences of technology, distinguishes the effects of social and procedural changes, and offers …


Stuck-At-Fault Immunity Enhancement Of Memristor-Based Edge Ai Systems, Md. Oli-Uz-Zaman Dec 2022

Stuck-At-Fault Immunity Enhancement Of Memristor-Based Edge Ai Systems, Md. Oli-Uz-Zaman

Graduate Theses and Dissertations (2019 - present)

Deep Neural Networks (DNN) are widely used in edge AI. But the complex perception and decision-making demand the overlarge computation and make the DNN architecture very sophisticated. Memristors have multilevel resistance property that enables faster in-memory DNN computation to remove the bottleneck caused by the von Neumann architecture and CMOS technology. However, the Stuck-At-Fault (SAF) defect of memristor generated from immature fabrication and heavy device utilization makes the memristor-based edge AI commercially unavailable. To mitigate this problem, an Adaptive Mapping Method (AMM) is proposed in this project. Based on the analysis for the VGG8 model with CIFAR10 dataset, the experiment …


Development Of Ultra-High Performance Engineered Geopolymer Composites (Uhp-Egcs), Hassan Noorvand, Miladin Radovic, Marwa Hassan, Svetlana Sukhishvili, Gabriel Arce, Ruwa Abufarsakh, Adriana A. Alvarado, Oscar Huang, Sang Zhen Dec 2022

Development Of Ultra-High Performance Engineered Geopolymer Composites (Uhp-Egcs), Hassan Noorvand, Miladin Radovic, Marwa Hassan, Svetlana Sukhishvili, Gabriel Arce, Ruwa Abufarsakh, Adriana A. Alvarado, Oscar Huang, Sang Zhen

Publications

This study investigated the possibility of developing novel UHP-EGC materials for the repair and new construction of transportation infrastructure in Region 6 by utilizing locally available resources. To this end, the geopolymers (GPs) in this study were synthesized by activating metakaolin (MK) with potassium silicate and sodium silicate solutions. The solutions were manufactured in the laboratory by dissolving silica fume and potassium hydroxide (KOH) or sodium hydroxide (NaOH) in deionized water. MK-based GP binders, mortars, and fiber-reinforced composites were manufactured and evaluated to determine their density, compressive strength, tensile properties, and slant shear bond strength to Portland cement concrete (PCC). …


Machine Learning For Early Detection Of Pediatric Sepsis, Glory Manson-Endeboh Dec 2022

Machine Learning For Early Detection Of Pediatric Sepsis, Glory Manson-Endeboh

Graduate Theses and Dissertations

Sepsis is a host response to infection in both adults and children. It contributes to approximately 1.7 million cases annually with nearly 270,000 annual deaths in the United States. In the United States, the financial burden of sepsis on survivors and their families including the hospitals is over $4.8 billion, at approximately $64,280 per hospitalization. The first goal of this thesis research is to develop efficient machine learning models to predict pediatric sepsis accurately for inpatients. The second objective is to develop machine learning methods to determine how early sepsis can be detected to mitigate mortality. We examine data collected …


Hybrid Multilevel Converters With Internal Cascaded/Paralleled Structures For Mv Electric Aircraft Applications, Fei Diao Dec 2022

Hybrid Multilevel Converters With Internal Cascaded/Paralleled Structures For Mv Electric Aircraft Applications, Fei Diao

Graduate Theses and Dissertations

Using on-board medium voltage (MV) dc distribution system has been a megatrend for next-generation electric aircraft systems due to its ability to enable a significant system mass reduction. In addition, it makes electric propulsion more feasible using MV power electronic converters. To develop high-performance high-density MV power converters, the emerging silicon carbide (SiC) devices are more attractive than their silicon (Si) counterparts, since the fast switch frequency brought by the SiC can effectively reduce the volume and weight of the filter components and thus increase the converter power density. From the converter topology perspective, with the MV dc distribution, the …


Development And Characterization Of Forcespinning® Mesquite Gum Nanofibers, Cristobal Rodriguez, Victoria Padilla, Karen Lozano, Alexa Villarreal, Luis A. Materon, Robert Gilkerson Dec 2022

Development And Characterization Of Forcespinning® Mesquite Gum Nanofibers, Cristobal Rodriguez, Victoria Padilla, Karen Lozano, Alexa Villarreal, Luis A. Materon, Robert Gilkerson

Mechanical Engineering Faculty Publications

In this study, nanofibers incorporating Prosopis glandulosa mesquite gum (MG) exudate combined with biodegradable polymers, pullulan (PL) and chitosan (CH), were produced via the Forcespinning® technique. The nonwoven composite membranes were characterized via scanning electron microscopy followed by thermogravimetric and Fourier-transform infrared spectroscopy analysis. MG nanofiber composites comprised long, continuous fibers with an average fiber diameter of 523 ± 180 nm and 760 ± 225 nm for 18.1 and 28 wt% of MG, respectively. These composite membranes were water-stable after crosslinking via heat treatment with a ∼3 % water absorption capacity, and demonstrated a thermal capability with an increasing …


Review Of Vegetable Nanolubricants For Tribological Applications, Laura Peña-Parás, Martha Rodríguez-Villalobos, Demófilo Maldonado-Cortés, José Andrés González, Ricardo De Jesús Durán, Javier A. Ortega Dec 2022

Review Of Vegetable Nanolubricants For Tribological Applications, Laura Peña-Parás, Martha Rodríguez-Villalobos, Demófilo Maldonado-Cortés, José Andrés González, Ricardo De Jesús Durán, Javier A. Ortega

Mechanical Engineering Faculty Publications

About 40 million tons of lubricants are used every year for various industry purposes and applications, where most of them are petroleum based oils. These oils are difficult and expensive to dispose of, have low biodegradability and are contamination risks. Recent efforts have been focused on reducing the environmental impact of petroleum based lubricants through the use of vegetable oils since they are biodegradable and have good lubricity. A drawback of vegetable oils is their poor thermal stability and oxidation, which causes them to decrease their properties at higher loads. Nanoparticle (NP) additives have been explored for improving the tribological …


Carbon Fibers From Bio-Based Precursors Derived From Renewable Sources, Sagar Kanhere Dec 2022

Carbon Fibers From Bio-Based Precursors Derived From Renewable Sources, Sagar Kanhere

All Dissertations

Carbon fibers have the highest strength and modulus among all known fibers and are used as reinforcements in high-performance composites [1]. Carbon fibers also have a very low density relative to metals. Therefore, carbon fibers possess ultrahigh specific strength and modulus, which make them desirable for high-performance light-weight composites. A vast majority of commercial carbon fibers are produced from PAN precursors that are expensive, which limits the use of PAN-derived carbon fibers to aerospace applications (e.g., airplanes). However, for costsensitive applications, there is a need for low-cost, moderate performance carbon fibers. Lignin is a low-cost by-product of pulping and biorefining …


Development Of Enzyme-Responsive Polymersomes As A Drug Delivery System For Gm1 Gangliosidosis, Bipin Chakravarthy Paruchuri Dec 2022

Development Of Enzyme-Responsive Polymersomes As A Drug Delivery System For Gm1 Gangliosidosis, Bipin Chakravarthy Paruchuri

All Dissertations

GM1 gangliosidosis is a lysosomal storage disorder caused by deficiency of β-galactosidase (βgal) and subsequent accumulation of GM1 ganglioside in lysosomes. The rare nature of this disorder presents challenges with prognosis and treatment. Enzyme replacement therapy (ERT) can effectively treat systemic deficiencies, but the intravenously administered enzyme cannot cross the blood-brain barrier (BBB) to treat the central nervous system. A delivery system to encapsulate and deliver the enzyme can facilitate enzyme transport across the BBB with suitable surface modifications. One of the pathological aspects of GM1-affected cells is the upregulation of the lysosomal enzyme hexosaminidase A (HexA). In this dissertation, …


Profiling And Engineering The Unfolded Protein Response In Therapeutic Protein-Producing Cho Cell Lines, Dyllan Rives Dec 2022

Profiling And Engineering The Unfolded Protein Response In Therapeutic Protein-Producing Cho Cell Lines, Dyllan Rives

All Dissertations

Industrial Chinese hamster ovary (CHO) production of therapeutics requires cell lines with high secretory capacity to avoid an accumulation of improperly folded proteins, or endoplasmic reticulum (ER) stress. This presents a challenging engineering bottleneck. The unfolded protein response (UPR) is initiated to overcome ER stress and reestablish homeostasis. In this dissertation, the impacts of ER stress and the UPR on protein production in mammalian cells are detailed, and both selection- and rational-based strategies for enhancing the ER stress response in productive CHO cell lines are reviewed. This project aims to expand on recent research efforts for engineering ER stress-related responses …